>VHB285 (VHB285Q) /CSM/VH/VHB2-D/VHB285Q.Seq.d/
AAAGATATTGATTTCACCAATTAGAAGAATAAAGGATGAATTTAAATTTCAATTCTACCC
AATTAATGAATGAGAATTTCAATGATGGAGATTATAGCGGATACAATAATGGAGGTATGA
TGATGAATAATAATAATAATAATAATCATAATAATCATAATAATAATCATAATAGTGGAA
TGAATGATATGAGAAATCAAATTGAGGATAGAATATTTGAATTAGAGAAAATTGAATATA
ATAAAGATGGTATCACATCAATTGTAGTAAATAATGGTAAAATGGCAATGACAACAAGAA
ATAATTATATCATTCGTTTGGATTTAGCTGATTCTATACCCTATGAAATTAAAATGAATA
GTGATATTTATAAAATATTTTGTGATCCTACAGGTAATCATATGGTTATTTGTATGACAA
ATGAAGAGATTTATTATTTACATACACAATCAACATCAAGAAAACCAAAACAATTAAAGA
TTAAAGTCGATTTAATTGAATCAATTGCATGGGATCCAGCTGGTGATAAAAATAATGTTC
AAACAATTTTAATTGGTACAAATAAAGGTAAAGTCTATGAAACTGTTGTTAGTGGTGTTG
AAAAAGGXXXXXXXXXX

ry*fhqlee*RMNLNFNSTQLMNENFNDGDYSGYNNGGMMMNNNNNNNHNNHNNNHNSGM
NDMRNQIEDRIFELEKIEYNKDGITSIVVNNGKMAMTTRNNYIIRLDLADSIPYEIKMNS
DIYKIFCDPTGNHMVICMTNEEIYYLHTQSTSRKPKQLKIKVDLIESIAWDPAGDKNNVQ
TILIGTNKGKVYETVVSGVEK---

Frame A:
kdidftn*knkg*i*isilpn**mrismmeiiadtimev***iiiiiiiiiiiiiiiive
*mi*eiklrieyln*rklniikmvshql**imvkwq*qqeiiisfvwi*lilypmklk*i
vifikyfvilqviiwlfv*qmkrfiiyihnqhqenqnn*rlksi*lnqlhgiqlvikimf
kqf*lvqikvksmklllvvlkk---


Frame B:
kilispirrikdefkfqfypine*efq*wrl*riq*wrydde******s**s***s**wn
e*yeksn*g*ni*iren*i**rwyhincsk*w*ngndnkk*lyhsfgfs*fytl*n*ne*
*yl*nil*syr*sygylydk*rdllftytinikktktikd*srfn*incmgssw**k*cs
nnfnwyk*r*sl*ncc*wc*k---


Frame C:
ry*fhqlee*RMNLNFNSTQLMNENFNDGDYSGYNNGGMMMNNNNNNNHNNHNNNHNSGM
NDMRNQIEDRIFELEKIEYNKDGITSIVVNNGKMAMTTRNNYIIRLDLADSIPYEIKMNS
DIYKIFCDPTGNHMVICMTNEEIYYLHTQSTSRKPKQLKIKVDLIESIAWDPAGDKNNVQ
TILIGTNKGKVYETVVSGVEK---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB285 (VHB285Q) /CSM/VH/VHB2-D/VHB285Q.Seq.d/                        858   0.0
SHA758 (SHA758Q) /CSM/SH/SHA7-C/SHA758Q.Seq.d/                        852   0.0
AHA312 (AHA312Q) /CSM/AH/AHA3-A/AHA312Q.Seq.d/                        846   0.0
VHC229 (VHC229Q) /CSM/VH/VHC2-B/VHC229Q.Seq.d/                        819   0.0
AHB810 (AHB810Q) /CSM/AH/AHB8-A/AHB810Q.Seq.d/                        815   0.0
VHC879 (VHC879Q) /CSM/VH/VHC8-D/VHC879Q.Seq.d/                        809   0.0
SHH467 (SHH467Q) /CSM/SH/SHH4-C/SHH467Q.Seq.d/                        803   0.0
SHL443 (SHL443Q) /CSM/SH/SHL4-B/SHL443Q.Seq.d/                        767   0.0
SHE811 (SHE811Q) /CSM/SH/SHE8-A/SHE811Q.Seq.d/                        765   0.0
SHG126 (SHG126Q) /CSM/SH/SHG1-B/SHG126Q.Seq.d/                        763   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CU234203|CU234203.1 Pig DNA sequence *** SEQUENCING IN PROGRESS *** from clone  CH242-258K16.    50   0.073  1
BX538353|BX538353.1 Cryptosporidium parvum chromosome 6, complete sequence; segment 4/4.    36   0.11   6
AE009951|AE009951.2 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, complete genome.    36   0.22   16
CP000102|CP000102.1 Methanosphaera stadtmanae DSM 3091, complete genome.    38   0.33   15
CR027367|CR027367.1 Reverse strand read from insert in 3'HPRT insertion targeting and chromosome engineering clone MHPP372h24.    42   0.63   2
AJ010592|AJ010592.2 Guillardia theta DNA for complete sequence of nucleomorph chromosome 2.    32   0.66   8
AF260826|AF260826.1 Cochliomyia hominivorax mitochondrion, complete genome.    40   0.69   2
ED062876|ED062876.1 AUAC-aab20d11.b1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    34   0.71   3
EJ298900|EJ298900.1 1095388065160 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061005534940 5', genomic survey sequence.    46   1.1    1
AG304851|AG304851.1 Mus musculus molossinus DNA, clone:MSMg01-085D04.T7, genomic survey sequence.    46   1.1    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM920436_1753(AM920436|pid:none) Penicillium chrysogenum Wiscons...    74   3e-12
BC154756_1(BC154756|pid:none) Danio rerio vacuolar protein sorti...    74   3e-12
AF506204_1(AF506204|pid:none) Danio rerio vacuolar protein sorti...    74   3e-12
(P59015) RecName: Full=Vacuolar protein sorting-associated prote...    74   3e-12
BC150096_1(BC150096|pid:none) Bos taurus vacuolar protein sortin...    73   6e-12
(Q8R307) RecName: Full=Vacuolar protein sorting-associated prote...    72   1e-11
BC169083_1(BC169083|pid:none) Rattus norvegicus vacuolar protein...    72   1e-11
AK029109_1(AK029109|pid:none) Mus musculus 10 days neonate skin ...    72   1e-11
BC039043_1(BC039043|pid:none) Mus musculus vacuolar protein sort...    72   1e-11
AK044821_1(AK044821|pid:none) Mus musculus 9.5 days embryo parth...    72   1e-11

 psg: 0.73  gvh: 0.07  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for VHB285 is nuc

>VHB285F.Seq
AAAGATATTGATTTCACCAATTAGAAGAATAAAGGATGAATTTAAATTTCAATTCTACCC
AATTAATGAATGAGAATTTCAATGATGGAGATTATAGCGGATACAATAATGGAGGTATGA
TGATGAATAATAATAATAATAATAATCATAATAATCATAATAATAATCATAATAGTGGAA
TGAATGATATGAGAAATCAAATTGAGGATAGAATATTTGAATTAGAGAAAATTGAATATA
ATAAAGATGGTATCACATCAATTGTAGTAAATAATGGTAAAATGGCAATGACAACAAGAA
ATAATTATATCATTCGTTTGGATTTAGCTGATTCTATACCCTATGAAATTAAAATGAATA
GTGATATTTATAAAATATTTTGTGATCCTACAGGTAATCATATGGTTATTTGTATGACAA
ATGAAGAGATTTATTATTTACATACACAATCAACATCAAGAAAACCAAAACAATTAAAGA
TTAAAGTCGATTTAATTGAATCAATTGCATGGGATCCAGCTGGTGATAAAAATAATGTTC
AAACAATTTTAATTGGTACAAATAAAGGTAAAGTCTATGAAACTGTTGTTAGTGGTGTTG
AAAAAGGNNNNNNNNNN