>VHB267 (VHB267Q) /CSM/VH/VHB2-C/VHB267Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCXXXXXXXXXXCACGAATGGGATAA
AAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGT
CAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTT
CCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAA
TCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCC
AACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACC
AATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACT
CAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTC
TGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCA
CACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGT
CAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGC
CCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACT
TTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAA

LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVS---

---HEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDE
NMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENA
IGGIYSVLNRRXGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFD
HWASIGVVNKDKKATEVALATRXRKGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kk
f*kk

Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmslyp---

---HEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDE
NMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENA
IGGIYSVLNRRXGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFD
HWASIGVVNKDKKATEVALATRXRKGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kk
f*kk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chci---

---tngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmk
t*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqlx*nqxt**kllpqktl
lvvftlysivvxvlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssi
iglqlvlstkirkplksl*ppexekv*pqksqiltnstknskqfkshfefskivikskkn
fkkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVS---

---rmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**k
herypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnxxrtnxlsrnycprkry
wwyllctqssxrycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrs
lgfnwccqqr*esh*srfshqkxkrfsprnprs*qiprktlnnlsrtlsfqk**snlkki
lkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB267 (VHB267Q) /CSM/VH/VHB2-C/VHB267Q.Seq.d/                       2545   0.0
VHH827 (VHH827Q) /CSM/VH/VHH8-B/VHH827Q.Seq.d/                       1364   0.0
VHE120 (VHE120Q) /CSM/VH/VHE1-A/VHE120Q.Seq.d/                       1364   0.0
VHB630 (VHB630Q) /CSM/VH/VHB6-B/VHB630Q.Seq.d/                       1364   0.0
VHA626 (VHA626Q) /CSM/VH/VHA6-B/VHA626Q.Seq.d/                       1364   0.0
VHA235 (VHA235Q) /CSM/VH/VHA2-B/VHA235Q.Seq.d/                       1364   0.0
CHE746 (CHE746Q) /CSM/CH/CHE7-B/CHE746Q.Seq.d/                       1362   0.0
VHP213 (VHP213Q) /CSM/VH/VHP2-A/VHP213Q.Seq.d/                       1358   0.0
VHO652 (VHO652Q) /CSM/VH/VHO6-C/VHO652Q.Seq.d/                       1358   0.0
VHO565 (VHO565Q) /CSM/VH/VHO5-C/VHO565Q.Seq.d/                       1358   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1219   0.0    1
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   2e-70  9
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   5e-69  9
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    72   7e-69  12
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    70   7e-66  10
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    64   6e-49  8
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   4e-47  6
AY144999|AY144999.1 Saccharomyces kluyveri clone Contig1706 EFT2 gene, complete cds.    78   4e-45  7
AR550596|AR550596.1 Sequence 5727 from patent US 6747137.          78   2e-44  6
EH015431|EH015431.1 USDA-FP_188032 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt081_G08 5', mRNA sequence.    78   6e-43  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   280   9e-74
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   279   2e-73
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   278   4e-73
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   278   4e-73
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   277   7e-73
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   277   7e-73
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   276   1e-72
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   275   4e-72
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   275   4e-72
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   275   4e-72

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHB267 is nuc

>VHB267F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCNNNNNNNNNN

>VHB267Z.Seq
NNNNNNNNNNCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGA
AGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAAT
TAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGA
AAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCA
CAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAAC
TGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGC
TATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACG
TCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGG
TTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGA
TCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGC
CACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAA
ACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAA
ATTTTAAAAAAAAAA

>VHB267P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCC----------CACGAATGGGATAA
AAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGT
CAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTT
CCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAA
TCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCC
AACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACC
AATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACT
CAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTC
TGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCA
CACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGT
CAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGC
CCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACT
TTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAA