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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB253
Library VH
(Link to library)
Clone ID VHB253
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15722-1
Original site URL
Representative seq. ID VHB253P
(Link to Original site)
Representative DNA sequence 
>VHB253 (VHB253Q) /CSM/VH/VHB2-C/VHB253Q.Seq.d/
CAATTTAAAAAAAATAATAGGAAAAAACACACCAAAATTAAATAAGTAANAATAAAAATG
AAATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAA
AAGTATTGCACTTATAGTGGTGNTGGAGAAGATTATAATATCAAATTTGATGTCTTACAA
ACATATGGTGTTGGTTTNCATTACTCATATTNAAGTGTTATTTTTACAACTTGTNATTAT
ACTATTCCTTGTGGTANTAATAGTNNTAATGTATNGCGTGGATCATATTCAATTGTTCCA
CGCACAGTATCTTTCATTGGGATTNGTTAATGGCAGXXXXXXXXXXATTGTTNGACACCA
AATGGTNCTTGTGATAATNATACTGGNGATTGTACATGCCATAATGAACATGTTGGTAAT
GGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGATATT
AATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAAATTT
ATTGAATGTAAACATNAATGTTCAACCAAACATGGAATATGTGATAATGATAGTGGTAAT
TGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTCATTGAA
TCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGGAAAT
NCAACATCATTATTAACAATTAAAATTGGTNAATCACAATGTAAAAATATCAAAGTATTC
AATGAAACANCATTACAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTACAAT
CATTGATGATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCTCNATGAAAAAAC
AAAATATCTCAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGGTTGTGTTGTTG
CTGTTTCATTACTTGGTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTTAATC
TTTTCTTCTAAAAACNAAAATAAAATTAACCATT
sequence update 2002.10.25
Translated Amino Acid sequence 
nlkkiigkntpklnk*x*k*niy*yiytyyyfvy*RSIAKSIALIVVXEKIIISNLMSYK
HMVLVXITHIXVLFLQLVIILFLVVXIVXMYXVDHIQLFHAQYLSLGXVNG---

---CXTPNGXCDNXTGDCTCHNEHVGNGCEFTQCPLYCSTPNGTCDINSGICTCDNEHIG
NGCEIKFIECKHXCSTKHGICDNDSGNCKCDTQTKGLTCEESRLFIESLDSINSKGGTIN
IIGYFGNXTSLLTIKIGXSQCKNIKVFNETXLQM*yw*r*rys*yynh***pfvhsnq*v
pisx*knkisqkyrevqllvlllvvlllfhylvlllfiiitkikrlifssknxnkinh
Translated Amino Acid sequence (All Frames) 
Frame A:
qfkknnrkkhtkik*vxikmkyllvylhillfcllkinsqkyctysgxgedynikfdvlq
tygvgxhysyxsvifttcxytipcgxnsxnvxrgsysivprtvsfigix*wq---

---ivxhqmvlvixilxivhaimnmlvmvvnslnahsivqhqmvlvilivvfvhvimnil
vmvaklnllnvnxnvqpnmeyvimivvivnvilkqkd*rvknqdyslnh*tqsiqkvvq*
i*lvilexqhhy*qlklvnhnvkiskysmkxhykcdigegkgihnitiidddlsftaink
fqylxektkylkniercncwyccwlccccfitwfycfllssqk*kg*sfllktkiklti

Frame B:
nlkkiigkntpklnk*x*k*niy*yiytyyyfvy*RSIAKSIALIVVXEKIIISNLMSYK
HMVLVXITHIXVLFLQLVIILFLVVXIVXMYXVDHIQLFHAQYLSLGXVNG---

---lxdtkwxl**xywxlymp**tcw*wl*ihsmptllfntkwyl*y**wylym***tyw
*wlrn*iy*m*txmfnqtwnm****w*l*m*ysnkrinv*rikiih*iirlnqfkrwynk
ynwlfwkxniiinn*nwxitm*kyqsiq*nxitnvilvkvkvfiilqslmmtfrsqqsis
snismkkqniskisrgaiagivvgcvvavsllgsiafyyhhknkkvnlff*kxk*n*p

Frame C:
i*kk**ekthqn*isxnkneifisifthitilfikdq*pkvlhl*wxwrrl*yqi*cltn
iwcwfxllixkcyfynlxlyyslwx**x*cxawiifncsthsifhwdxlma---

---CXTPNGXCDNXTGDCTCHNEHVGNGCEFTQCPLYCSTPNGTCDINSGICTCDNEHIG
NGCEIKFIECKHXCSTKHGICDNDSGNCKCDTQTKGLTCEESRLFIESLDSINSKGGTIN
IIGYFGNXTSLLTIKIGXSQCKNIKVFNETXLQM*yw*r*rys*yynh***pfvhsnq*v
pisx*knkisqkyrevqllvlllvvlllfhylvlllfiiitkikrlifssknxnkinh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB253 (VHB253Q) /CSM/VH/VHB2-C/VHB253Q.Seq.d/                       1691   0.0
VHQ484 (VHQ484Q) /CSM/VH/VHQ4-D/VHQ484Q.Seq.d/                       1181   0.0
VHQ244 (VHQ244Q) /CSM/VH/VHQ2-B/VHQ244Q.Seq.d/                       1181   0.0
VHQ143 (VHQ143Q) /CSM/VH/VHQ1-B/VHQ143Q.Seq.d/                       1181   0.0
VHP829 (VHP829Q) /CSM/VH/VHP8-B/VHP829Q.Seq.d/                       1181   0.0
VHP821 (VHP821Q) /CSM/VH/VHP8-A/VHP821Q.Seq.d/                       1181   0.0
VHP763 (VHP763Q) /CSM/VH/VHP7-C/VHP763Q.Seq.d/                       1181   0.0
VHP673 (VHP673Q) /CSM/VH/VHP6-D/VHP673Q.Seq.d/                       1181   0.0
VHP548 (VHP548Q) /CSM/VH/VHP5-B/VHP548Q.Seq.d/                       1181   0.0
VHP522 (VHP522Q) /CSM/VH/VHP5-A/VHP522Q.Seq.d/                       1181   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116924|AC116924.3 Dictyostelium discoideum chromosome 2 map 6357117-6445670 strain AX4, complete sequence.    82   1e-33  6
AC115592|AC115592.2 Dictyostelium discoideum chromosome 2 map 1-125959 strain AX4, complete sequence.    76   4e-30  6
AC117076|AC117076.3 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    94   9e-15  1
AY392441|AY392441.1 Dictyostelium discoideum possible blackjack gene, complete cds.    72   3e-08  1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    48   3e-05  2
AP007175|AP007175.1 Aspergillus oryzae RIB40 genomic DNA, SC010.    62   3e-05  1
AC115683|AC115683.2 Dictyostelium discoideum chromosome 2 map complement(6135149-6105997) strain AX4, complete sequence.    46   0.003  4
AP006986|AP006986.1 Saccharum edule chloroplast DNA, intergenic region between trnT and trnL, strain: COL/PAPUA N.G/TARC/E12, clone: EPN-46kb.    46   0.021  2
AP006985|AP006985.1 Saccharum edule chloroplast DNA, intergenic region between trnT and trnL, strain: IN95-009, clone: EIN-46kb.    46   0.021  2
AP006984|AP006984.1 Saccharum sinense chloroplast DNA, intergenic region between trnT and trnL, strain: Ooshima, clone: SiO-46kb.    46   0.021  2
dna update 2007. 3.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117075_64(AC117075|pid:none) Dictyostelium discoideum chromoso...    86   2e-21
AC116100_9(AC116100|pid:none) Dictyostelium discoideum chromosom...    91   3e-19
AC116963_42(AC116963|pid:none) Dictyostelium discoideum chromoso...    89   1e-18
AC116920_20(AC116920|pid:none) Dictyostelium discoideum chromoso...    97   1e-18
AC116920_24(AC116920|pid:none) Dictyostelium discoideum chromoso...    94   1e-17
AC116967_7(AC116967|pid:none) Dictyostelium discoideum chromosom...    92   2e-17
AC116967_8(AC116967|pid:none) Dictyostelium discoideum chromosom...    90   2e-16
AC116956_34(AC116956|pid:none) Dictyostelium discoideum chromoso...    84   2e-16
AC116920_25(AC116920|pid:none) Dictyostelium discoideum chromoso...    85   5e-15
AC117081_39(AC117081|pid:none) Dictyostelium discoideum chromoso...    84   1e-14
protein update 2009. 6.27
PSORT 

 psg: 0.92  gvh: 0.58  alm: 0.10  top: 0.40  tms: 0.07  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.14  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 12.0 %: Golgi
	 12.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for VHB253 is end
5' end seq. ID VHB253F
5' end seq. 
>VHB253F.Seq
CAATTTAAAAAAAATAATAGGAAAAAACACACCAAAATTAAATAAGTAANAATAAAAATG
AAATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAA
AAGTATTGCACTTATAGTGGTGNTGGAGAAGATTATAATATCAAATTTGATGTCTTACAA
ACATATGGTGTTGGTTTNCATTACTCATATTNAAGTGTTATTTTTACAACTTGTNATTAT
ACTATTCCTTGTGGTANTAATAGTNNTAATGTATNGCGTGGATCATATTCAATTGTTCCA
CGCACAGTATCTTTCATTGGGATTNGTTAATGGCAGNNNNNNNNNN
Length of 5' end seq. 346
3' end seq. ID VHB253Z
3' end seq. 
>VHB253Z.Seq
NNNNNNNNNNATTGTTNGACACCAAATGGTNCTTGTGATAATNATACTGGNGATTGTACA
TGCCATAATGAACATGTTGGTAATGGTTGTGAATTCACTCAATGCCCACTCTATTGTTCA
ACACCAAATGGTACTTGTGATATTAATAGTGGTATTTGTACATGTGATAATGAACATATT
GGTAATGGTTGCGAAATTAAATTTATTGAATGTAAACATNAATGTTCAACCAAACATGGA
ATATGTGATAATGATAGTGGTAATTGTAAATGTGATACTCAAACAAAAGGATTAACGTGT
GAAGAATCAAGATTATTCATTGAATCATTAGACTCAATCAATTCAAAAGGTGGTACAATA
AATATAATTGGTTATTTTGGAAATNCAACATCATTATTAACAATTAAAATTGGTNAATCA
CAATGTAAAAATATCAAAGTATTCAATGAAACANCATTACAAATGTGATATTGGTGAAGG
TAAAGGTATTCATAATATTACAATCATTGATGATGACCTTTCGTTCACAGCAATCAATAA
GTTCCAATATCTCNATGAAAAAACAAAATATCTCAAAAATATCGAGAGGTGCAATTGCTG
GTATTGTTGTTGGTTGTGTTGTTGCTGTTTCATTACTTGGTTCTATTGCTTTTTATTATC
ATCACAAAAATAAAAAGGTTAATCTTTTCTTCTAAAAACNAAAATAAAATTAACCATT
Length of 3' end seq. 718
Connected seq. ID VHB253P
Connected seq. 
>VHB253P.Seq
CAATTTAAAAAAAATAATAGGAAAAAACACACCAAAATTAAATAAGTAANAATAAAAATG
AAATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAA
AAGTATTGCACTTATAGTGGTGNTGGAGAAGATTATAATATCAAATTTGATGTCTTACAA
ACATATGGTGTTGGTTTNCATTACTCATATTNAAGTGTTATTTTTACAACTTGTNATTAT
ACTATTCCTTGTGGTANTAATAGTNNTAATGTATNGCGTGGATCATATTCAATTGTTCCA
CGCACAGTATCTTTCATTGGGATTNGTTAATGGCAG----------ATTGTTNGACACCA
AATGGTNCTTGTGATAATNATACTGGNGATTGTACATGCCATAATGAACATGTTGGTAAT
GGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGATATT
AATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAAATTT
ATTGAATGTAAACATNAATGTTCAACCAAACATGGAATATGTGATAATGATAGTGGTAAT
TGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTCATTGAA
TCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGGAAAT
NCAACATCATTATTAACAATTAAAATTGGTNAATCACAATGTAAAAATATCAAAGTATTC
AATGAAACANCATTACAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTACAAT
CATTGATGATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCTCNATGAAAAAAC
AAAATATCTCAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGGTTGTGTTGTTG
CTGTTTCATTACTTGGTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTTAATC
TTTTCTTCTAAAAACNAAAATAAAATTAACCATT
Length of connected seq. 1044
Full length Seq ID -
Full length Seq. -
Length of full length seq. -