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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB245
Library VH
(Link to library)
Clone ID VHB245
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12067-1
Original site URL
Representative seq. ID VHB245P
(Link to Original site)
Representative DNA sequence 
>VHB245 (VHB245Q) /CSM/VH/VHB2-B/VHB245Q.Seq.d/
GCAGGTACAACCGCTCAAGCAAGTCCAATCAAATAATAGTTCTTATAATGCTAAAGATGA
TGATGATGATGAACCAGGTTCAAGTGGTAATGATATTCAAAAGATTTTAGGTAAAGAAAG
ATTAGGTCGTATTCTTTCATCATTGTATATGATGAGATTTGATAATAATTCATCAGTTCG
TCAAAANGNATTATTAATTTGGAAATATATTGXXXXXXXXXXGAAAAGAAACCAAAAGTT
GANCAAGCTACACTCGATAAAGCAATCGCCACCATTGTGGACTTCCTTTATCAACNTTCC
GATGATCTCAGATCAATGGTCGCTCAAACTATTGGTGCCTCTTCAAAATGTTTTACTTCT
CTCACTGAATTGAATCAATTCATTAAAACCAATCTCATCTCACCTTCTCAATCAGTACTC
TCTCGTTATGGTAAATCATTGGCATTGGGTGAAATTTTCAAAGCATCTGGTAAGAATTTA
ATCGATTCTCAATCACCAAATATGCCAACCATCATTAAAATCATTCAAACCGATTGTCGT
GATGAAAAAGGTCCAATTCGTGAATCCTCTGCTTATTTGGCTGAAGCTATTTTAGTTGCT
TCGCCTTTAACCTATGCAAAGGATTTAGTACCTTCCATTTGTCATCTCATTGGTGATCAA
TCATCTTCCGTATCAATTAGTGCTCTCAATGTTATTAAACGTTTCTGTAAATCAAATCAA
CAATTATCAAGACAATATTTACGTGATATTGTCGTACCAACTATGAATAGATTAAAAGAA
AGAACTAATTTACCTCTAAAATTAGCTGCTGAAAGAACTTTAGTTCATTCACTTCAAATC
TTTAAAGAATCCATTGTAATGGATGATTTAATTAAACAATTAGAATTATCTGGTGATTCA
TCAATGGCAAATTCTTTAATTGATTATCATAAAAGAGTTTTAATAAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
QVQPLKQVQSNNSSYNAKDDDDDEPGSSGNDIQKILGKERLGRILSSLYMMRFDNNSSVR
QXXLLIWKYI---

---EKKPKVXQATLDKAIATIVDFLYQXSDDLRSMVAQTIGASSKCFTSLTELNQFIKTN
LISPSQSVLSRYGKSLALGEIFKASGKNLIDSQSPNMPTIIKIIQTDCRDEKGPIRESSA
YLAEAILVASPLTYAKDLVPSICHLIGDQSSSVSISALNVIKRFCKSNQQLSRQYLRDIV
VPTMNRLKERTNLPLKLAAERTLVHSLQIFKESIVMDDLIKQLELSGDSSMANSLIDYHK
RVLIK
Translated Amino Acid sequence (All Frames) 
Frame A:
agttaqaspik**fl*c*r*****trfkw**yskdfr*rkirsysfiivydei***fiss
skxiinleiy---

---EKKPKVXQATLDKAIATIVDFLYQXSDDLRSMVAQTIGASSKCFTSLTELNQFIKTN
LISPSQSVLSRYGKSLALGEIFKASGKNLIDSQSPNMPTIIKIIQTDCRDEKGPIRESSA
YLAEAILVASPLTYAKDLVPSICHLIGDQSSSVSISALNVIKRFCKSNQQLSRQYLRDIV
VPTMNRLKERTNLPLKLAAERTLVHSLQIFKESIVMDDLIKQLELSGDSSMANSLIDYHK
RVLIK

Frame B:
QVQPLKQVQSNNSSYNAKDDDDDEPGSSGNDIQKILGKERLGRILSSLYMMRFDNNSSVR
QXXLLIWKYI---

---krnqklxklhsikqspplwtsfinxpmisdqwslkllvplqnvlllsln*inslkpi
sshllnqyslvmvnhwhwvkfskhlvri*silnhqicqpslksfkpivvmkkvqfvnpll
iwlklf*llrl*pmqri*ylpfvislvinhlpyqlvlsmllnvsvnqinnyqdniyvils
yql*id*kkeliyl*n*llkel*fihfkslknpl*wmi*lnn*nylvihqwqil*liiik
ef**n

Frame C:
rynrssksnqiivlimlkmmmmmnqvqvvmifkrf*vkkd*vvffhhci**dliiihqfv
kxxy*fgnil---

---ketks*xsytr*snrhhcglplstfr*sqingrsnywclfkmfyfsh*iesih*nqs
hltfsistlslw*iigig*nfqsiw*efnrfsitkyanhh*nhsnrls**krsns*ilcl
fg*syfscfafnlckgfstfhlsshw*siifrin*csqcy*tfl*ikstiiktift*ycr
tnye*ikrkn*ftskisc*knfssftsnl*rihcng*fn*tiriiw*fingkffn*ls*k
sfnki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB245 (VHB245Q) /CSM/VH/VHB2-B/VHB245Q.Seq.d/                       1657   0.0
VHC675 (VHC675Q) /CSM/VH/VHC6-D/VHC675Q.Seq.d/                       1374   0.0
VHA441 (VHA441Q) /CSM/VH/VHA4-B/VHA441Q.Seq.d/                       1374   0.0
VHI374 (VHI374Q) /CSM/VH/VHI3-D/VHI374Q.Seq.d/                       1352   0.0
SHF505 (SHF505Q) /CSM/SH/SHF5-A/SHF505Q.Seq.d/                       1352   0.0
VHO589 (VHO589Q) /CSM/VH/VHO5-D/VHO589Q.Seq.d/                       1261   0.0
VHK689 (VHK689Q) /CSM/VH/VHK6-D/VHK689Q.Seq.d/                       1261   0.0
VHA755 (VHA755Q) /CSM/VH/VHA7-C/VHA755Q.Seq.d/                       1251   0.0
VHL277 (VHL277Q) /CSM/VH/VHL2-D/VHL277Q.Seq.d/                       1215   0.0
VHF215 (VHF215Q) /CSM/VH/VHF2-A/VHF215Q.Seq.d/                        957   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC119982|AC119982.6 Mus musculus chromosome 19, clone RP24-498L9, complete sequence.    56   0.002  1
AC118724|AC118724.18 Mus musculus, clone RP24-160H20, complete sequence.    56   0.002  1
BH167468|BH167468.1 ENTSH94TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.11   1
BH163191|BH163191.1 ENTTE33TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.11   1
AZ531303|AZ531303.1 ENTCH17TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50   0.11   1
ED460729|ED460729.1 AUAC-aao05g05.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    50   0.11   1
ED450392|ED450392.1 AUAC-aan56c12.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    50   0.11   1
ED393242|ED393242.1 AUAC-aai25f10.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    50   0.11   1
ED186094|ED186094.1 AUAC-aah66a04.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    50   0.11   1
AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    36   1.3    9
dna update 2007. 3.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54WR2) RecName: Full=Translational activator gcn1; AltName: Fu...   457   e-127
AC006193_10(AC006193|pid:none) Arabidopsis thaliana chromosome I...    66   2e-09
AP008209_2460(AP008209|pid:none) Oryza sativa (japonica cultivar...    61   5e-08
AC146936_30(AC146936|pid:none) Oryza sativa (japonica cultivar-g...    61   5e-08
(P33892) RecName: Full=Translational activator GCN1;  &A48126(A4...    50   1e-04
(Q10105) RecName: Full=Translational activator gcn1;  &CU329670_...    44   0.006
AM920428_489(AM920428|pid:none) Penicillium chrysogenum Wisconsi...    44   0.010
AL954693_2(AL954693|pid:none) Zebrafish DNA sequence from clone ...    44   0.010
AF377946_1(AF377946|pid:none) Oryza sativa (japonica cultivar-gr...    43   0.018
CU633454_76(CU633454|pid:none) Podospora anserina genomic DNA ch...    43   0.018
protein update 2009. 6.27
PSORT 

 psg: 0.65  gvh: 0.24  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB245 is nuc
5' end seq. ID VHB245F
5' end seq. 
>VHB245F.Seq
GCAGGTACAACCGCTCAAGCAAGTCCAATCAAATAATAGTTCTTATAATGCTAAAGATGA
TGATGATGATGAACCAGGTTCAAGTGGTAATGATATTCAAAAGATTTTAGGTAAAGAAAG
ATTAGGTCGTATTCTTTCATCATTGTATATGATGAGATTTGATAATAATTCATCAGTTCG
TCAAAANGNATTATTAATTTGGAAATATATTGNNNNNNNNNN
Length of 5' end seq. 222
3' end seq. ID VHB245Z
3' end seq. 
>VHB245Z.Seq
NNNNNNNNNNGAAAAGAAACCAAAAGTTGANCAAGCTACACTCGATAAAGCAATCGCCAC
CATTGTGGACTTCCTTTATCAACNTTCCGATGATCTCAGATCAATGGTCGCTCAAACTAT
TGGTGCCTCTTCAAAATGTTTTACTTCTCTCACTGAATTGAATCAATTCATTAAAACCAA
TCTCATCTCACCTTCTCAATCAGTACTCTCTCGTTATGGTAAATCATTGGCATTGGGTGA
AATTTTCAAAGCATCTGGTAAGAATTTAATCGATTCTCAATCACCAAATATGCCAACCAT
CATTAAAATCATTCAAACCGATTGTCGTGATGAAAAAGGTCCAATTCGTGAATCCTCTGC
TTATTTGGCTGAAGCTATTTTAGTTGCTTCGCCTTTAACCTATGCAAAGGATTTAGTACC
TTCCATTTGTCATCTCATTGGTGATCAATCATCTTCCGTATCAATTAGTGCTCTCAATGT
TATTAAACGTTTCTGTAAATCAAATCAACAATTATCAAGACAATATTTACGTGATATTGT
CGTACCAACTATGAATAGATTAAAAGAAAGAACTAATTTACCTCTAAAATTAGCTGCTGA
AAGAACTTTAGTTCATTCACTTCAAATCTTTAAAGAATCCATTGTAATGGATGATTTAAT
TAAACAATTAGAATTATCTGGTGATTCATCAATGGCAAATTCTTTAATTGATTATCATAA
AAGAGTTTTAATAAAATT
Length of 3' end seq. 738
Connected seq. ID VHB245P
Connected seq. 
>VHB245P.Seq
GCAGGTACAACCGCTCAAGCAAGTCCAATCAAATAATAGTTCTTATAATGCTAAAGATGA
TGATGATGATGAACCAGGTTCAAGTGGTAATGATATTCAAAAGATTTTAGGTAAAGAAAG
ATTAGGTCGTATTCTTTCATCATTGTATATGATGAGATTTGATAATAATTCATCAGTTCG
TCAAAANGNATTATTAATTTGGAAATATATTG----------GAAAAGAAACCAAAAGTT
GANCAAGCTACACTCGATAAAGCAATCGCCACCATTGTGGACTTCCTTTATCAACNTTCC
GATGATCTCAGATCAATGGTCGCTCAAACTATTGGTGCCTCTTCAAAATGTTTTACTTCT
CTCACTGAATTGAATCAATTCATTAAAACCAATCTCATCTCACCTTCTCAATCAGTACTC
TCTCGTTATGGTAAATCATTGGCATTGGGTGAAATTTTCAAAGCATCTGGTAAGAATTTA
ATCGATTCTCAATCACCAAATATGCCAACCATCATTAAAATCATTCAAACCGATTGTCGT
GATGAAAAAGGTCCAATTCGTGAATCCTCTGCTTATTTGGCTGAAGCTATTTTAGTTGCT
TCGCCTTTAACCTATGCAAAGGATTTAGTACCTTCCATTTGTCATCTCATTGGTGATCAA
TCATCTTCCGTATCAATTAGTGCTCTCAATGTTATTAAACGTTTCTGTAAATCAAATCAA
CAATTATCAAGACAATATTTACGTGATATTGTCGTACCAACTATGAATAGATTAAAAGAA
AGAACTAATTTACCTCTAAAATTAGCTGCTGAAAGAACTTTAGTTCATTCACTTCAAATC
TTTAAAGAATCCATTGTAATGGATGATTTAATTAAACAATTAGAATTATCTGGTGATTCA
TCAATGGCAAATTCTTTAATTGATTATCATAAAAGAGTTTTAATAAAATT
Length of connected seq. 940
Full length Seq ID -
Full length Seq. -
Length of full length seq. -