>VHB224 (VHB224Q) /CSM/VH/VHB2-A/VHB224Q.Seq.d/
XXXXXXXXXXTGGTATGAATATGGTNATGGTTCATTGGTTCAATCAGTTGGTACCCATCN
ANCTNTAAATGCTCCANTNGATGTAATCAACGTTCATANGCGTGGTGGTAATATCATTCC
AACTCAACCAACCTCCTCATATGTTACACCAGTTGATGGTATTCCAATTNCCACTAAAAT
CTCTAGACCTTTACCATTTGAATTGATTATTGCCTNGGATTNTTCATTACAAGCAACTGG
TCAATTATTNNTGGATGATGGTGAATCAATTCAAACCTATGTTGATAATAAATACTCTTT
CATTCAATTCGATGTTGTGGTCCTCACCATCTTCATCTGCNTACAAATTACAATCAACCA
TTCTCAATAACAATTATAATGGTACCGCTTCTTTAATCATTAATTCTATCCAAATGTACG
GTTCGCCATCAGTTCNACAAGTCATTGTTAATGGTAGCCCAATCAATTCATTTAATGCTG
TCTCNGATTCAACTCTCTCTGTTTCAAATNTACAACTTGCTTTAGATGAATCCT

---YEYGXGSLVQSVGTHXXXNAPXDVINVHXRGGNIIPTQPTSSYVTPVDGIPIXTKIS
RPLPFELIIAXDXSLQATGQLXXDDGESIQTYVDNKYSFIQFDVVVLTIFICXQITINHS
Q*ql*wyrffnh*fypnvrfaisstshc*w*pnqfi*cclxfnslcfkxttcfr*i

Frame A:
---gmnmvmvhwfnqlvpixl*mlxxm*stfixvvvisfqlnqpphmlhqlmvfqxplks
ldlyhln*llpxixhykqlvnyxwmmvnqfkpmliintlsfnsmlwsspsssayklqsti
lnnnyngtasliinsiqmygspsvxqvivngspinsfnavsdstlsvsnxqlaldes


Frame B:
---v*iwxwfigsiswypsxxkcsxxcnqrsxaww*yhsnstnllicyts*wysnxh*nl
*tfti*idyclgxfitsnwsiixg*w*insnlc***ilfhsirccgphhlhlxtnynqpf
sitiimvpll*slilskctvrhqfxksllmvaqsihlmlsxiqlslfqxynll*mnp


Frame C:
---YEYGXGSLVQSVGTHXXXNAPXDVINVHXRGGNIIPTQPTSSYVTPVDGIPIXTKIS
RPLPFELIIAXDXSLQATGQLXXDDGESIQTYVDNKYSFIQFDVVVLTIFICXQITINHS
Q*ql*wyrffnh*fypnvrfaisstshc*w*pnqfi*cclxfnslcfkxttcfr*i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB224 (VHB224Q) /CSM/VH/VHB2-A/VHB224Q.Seq.d/                        944   0.0
CHG312 (CHG312Q) /CSM/CH/CHG3-A/CHG312Q.Seq.d/                        898   0.0
VHA108 (VHA108Q) /CSM/VH/VHA1-A/VHA108Q.Seq.d/                        896   0.0
SHC731 (SHC731Q) /CSM/SH/SHC7-B/SHC731Q.Seq.d/                        896   0.0
CHS185 (CHS185Q) /CSM/CH/CHS1-D/CHS185Q.Seq.d/                        896   0.0
CHR858 (CHR858Q) /CSM/CH/CHR8-C/CHR858Q.Seq.d/                        896   0.0
CHR857 (CHR857Q) /CSM/CH/CHR8-C/CHR857Q.Seq.d/                        896   0.0
CHM276 (CHM276Q) /CSM/CH/CHM2-D/CHM276Q.Seq.d/                        896   0.0
CHH383 (CHH383Q) /CSM/CH/CHH3-D/CHH383Q.Seq.d/                        896   0.0
AHR217 (AHR217Q) /CSM/AH/AHR2-A/AHR217Q.Seq.d/                        896   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EC761186|EC761186.1 PSE00006759 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    54   0.004  1
AR544895|AR544895.1 Sequence 26 from patent US 6747137.            40   1.7    2
AJ013037|AJ013037.1 Entamoeba histolytica EST EhEST329.            44   3.9    1
EK112904|EK112904.1 1092963138958 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061002065041 3', genomic survey sequence.    44   3.9    1
AC098296|AC098296.7 Rattus norvegicus clone CH230-1I14, WORKING DRAFT SEQUENCE, 4 unordered pieces.    38   4.7    3
AR547126|AR547126.1 Sequence 2257 from patent US 6747137.          40   5.5    2
AF082188|AF082188.1 Candida albicans glucoamylase (GCA1) gene, complete cds.    40   7.2    2
DY301183|DY301183.1 KN0AAQ12YG10RM1 CitNFL Citrus clementina cDNA 5', mRNA sequence.    40   9.8    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L25926_1(L25926|pid:none) Rat sucrase-isomaltase (SI) mRNA, comp...    69   2e-11
(P23739) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    69   2e-11
EU937530_1(EU937530|pid:none) Mus musculus sucrase-isomaltase mR...    69   2e-11
AB000967_1(AB000967|pid:none) Coturnix japonica GAAI mRNA for ac...    68   1e-10
BC115034_1(BC115034|pid:none) Homo sapiens sucrase-isomaltase (a...    66   5e-10
(P14410) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    66   5e-10
BC116452_1(BC116452|pid:none) Homo sapiens sucrase-isomaltase (a...    66   5e-10
UUHU(S36082;A27326;S24329;A61136) sucrose alpha-glucosidase (EC ...    66   5e-10
AB428422_1(AB428422|pid:none) Felis catus SI mRNA for sucrase-is...    61   2e-08
AF016833_1(AF016833|pid:none) Homo sapiens maltase-glucoamylase ...    60   3e-08

 psg: 0.75  gvh: 0.40  alm: 0.29  top: 0.63  tms: 0.07  mit: 0.16  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHB224 is cyt

>VHB224Z.Seq
NNNNNNNNNNTGGTATGAATATGGTNATGGTTCATTGGTTCAATCAGTTGGTACCCATCN
ANCTNTAAATGCTCCANTNGATGTAATCAACGTTCATANGCGTGGTGGTAATATCATTCC
AACTCAACCAACCTCCTCATATGTTACACCAGTTGATGGTATTCCAATTNCCACTAAAAT
CTCTAGACCTTTACCATTTGAATTGATTATTGCCTNGGATTNTTCATTACAAGCAACTGG
TCAATTATTNNTGGATGATGGTGAATCAATTCAAACCTATGTTGATAATAAATACTCTTT
CATTCAATTCGATGTTGTGGTCCTCACCATCTTCATCTGCNTACAAATTACAATCAACCA
TTCTCAATAACAATTATAATGGTACCGCTTCTTTAATCATTAATTCTATCCAAATGTACG
GTTCGCCATCAGTTCNACAAGTCATTGTTAATGGTAGCCCAATCAATTCATTTAATGCTG
TCTCNGATTCAACTCTCTCTGTTTCAAATNTACAACTTGCTTTAGATGAATCCT