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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB221
Library VH
(Link to library)
Clone ID VHB221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11964-1
Original site URL
Representative seq. ID VHB221P
(Link to Original site)
Representative DNA sequence 
>VHB221 (VHB221Q) /CSM/VH/VHB2-A/VHB221Q.Seq.d/
CATTTTAGTAACAATCAAATATAAGATTTCTTATATTGGATACGTGGTGTACTTTTGTGT
TTGGGTGGATTTTATTATTTATTTATTTTAATCATCATTTTTATTTGTAATATATTTCAT
TTTTATCACACAAAACACACACAAAACACACACTCACAAAAACACACACTCACACATAAA
TAAATACACAATAATAAAAGAAGAAAGAAAATGGAGAAAAGAAAGAGTATATTTTTTGCA
AATGATAGTGAAGATAATACATCATCAGGAGATTTAAGTAGTTTTACAAAACGTGGTACA
ATTACACCAGGTAAGATTGCAAATGCACGTGAAGGAGAGTTATCACAAATGATTGATGTT
GAATCGGAACAGAAATATCAAGAAATTATTGAACTTTTAGTTACAGGTGGTTATTTTAGA
GCACGTATTTCAGGTTTATCACCATTTGATAAAGTTGTTGGTGGCATGGCTTGGTCAATC
ACTGCTTCAAATGTTGATGTTGACGCTGATTTATTCTTTCAAGAAAATTCAAATATTAAA
CAAAAAGTATCATTATCAGAAGAATTAATTAAAGCATTAAATCGTATGAAATGTCCATTT
XXXXXXXXXXACAAGCATTCAATGAACGTATCATTAGAGAGACAGAGAAATTGGATGCAT
TGGAAACACCAGAGAATACTAAAGCATTACAAGCACTACGTTCATTGGTACTATTGAATG
AAACATTGATTGCTCAAGAGGAACTTTTCAAACAATCTTGTAAACGTCAAATGGTAGAAT
ATAAACAAAAGATCGAACAACTCTTATCACAAGATGCCTCTACTCAAGAGGATCTTCAAG
ACAGTGATAGACAACAACAAATTCAACAAGCATTCGATAGTGATATTGGTAAATTAAAGA
AATTGAAATTATTACTCAATAAAAAGAATAGAGATATCTCTTTGATTCAACGTTATCTCG
ATGAATTACCATCTCGTGCCGAACTATTACAATATCAAAGACAATTTGTTGAATTATCCG
AACAAAGTAGTTCGAAATTAACCGAAACCAGACAATACTATACAACCTACAATACCTTTG
AAGATAAGAAAACACTTTTAGAAAATGAACTCTCAATTCTCAATTCAATTCAATCCAAAT
ATAGCATAGCTATGTCTTCAACAAATAATAAAGAATTATTCATTAAATCAATGGATCAAA
TCATTGATGGTATTCAACAAGTTTTAGATAAATCTGAAAATAAATGGAATGTTGAAAAAG
TTCGTCATGATACTTTATCTGATCAATATATGACTTTATTAGATCAAGAGAGAAATTATT
ATAAATTAACAAAAGACTTGAAGAAGAATGTAGAAAGAAGAATTATTAATTAAAAAATTA
AANAATTACAATAAATAAATAAATAAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
hfsnnqi*dflywirgvllclggfyylfiliiificnifhfyhtkhtqnthsqkhtlthk
*IHNNKRRKKMEKRKSIFFANDSEDNTSSGDLSSFTKRGTITPGKIANAREGELSQMIDV
ESEQKYQEIIELLVTGGYFRARISGLSPFDKVVGGMAWSITASNVDVDADLFFQENSNIK
QKVSLSEELIKALNRMKCPF---

---QAFNERIIRETEKLDALETPENTKALQALRSLVLLNETLIAQEELFKQSCKRQMVEY
KQKIEQLLSQDASTQEDLQDSDRQQQIQQAFDSDIGKLKKLKLLLNKKNRDISLIQRYLD
ELPSRAELLQYQRQFVELSEQSSSKLTETRQYYTTYNTFEDKKTLLENELSILNSIQSKY
SIAMSSTNNKELFIKSMDQIIDGIQQVLDKSENKWNVEKVRHDTLSDQYMTLLDQERNYY
KLTKDLKKNVERRIIN*kikxlq*ink*i
Translated Amino Acid sequence (All Frames) 
Frame A:
hfsnnqi*dflywirgvllclggfyylfiliiificnifhfyhtkhtqnthsqkhtlthk
*IHNNKRRKKMEKRKSIFFANDSEDNTSSGDLSSFTKRGTITPGKIANAREGELSQMIDV
ESEQKYQEIIELLVTGGYFRARISGLSPFDKVVGGMAWSITASNVDVDADLFFQENSNIK
QKVSLSEELIKALNRMKCPF---

---tsiq*tyh*rdreigcigntrey*sitsttfigtie*nidcsrgtfqtil*tsngri
*tkdrttlitrclysrgssrq**tttnstsir**yw*ikeieiitq*ke*rylfdstlsr
*itiscrtitisktic*iirtk*feinrnqtilynlqyl*r*entfrk*tlnsqfnsiqi
*hsyvfnk**riih*ingsnh*wystsfr*i*k*mec*ksss*yfi*siydfirsrekll
*inkrleeecrkkny*lkn*xitink*in

Frame B:
ilvtikykisyigyvvyfcvwvdfiiylf*ssflfviyfifitqnthkththknthshin
kytiikeerkwrkervyflqmivkiihhqei*vvlqnvvqlhqvrlqmhvkesyhk*lml
nrnrnikkllnf*lqvvilehvfqvyhhlikllvawlgqsllqmlmltliysfkkiqiln
kkyhyqkn*lkh*iv*nvh---

---QAFNERIIRETEKLDALETPENTKALQALRSLVLLNETLIAQEELFKQSCKRQMVEY
KQKIEQLLSQDASTQEDLQDSDRQQQIQQAFDSDIGKLKKLKLLLNKKNRDISLIQRYLD
ELPSRAELLQYQRQFVELSEQSSSKLTETRQYYTTYNTFEDKKTLLENELSILNSIQSKY
SIAMSSTNNKELFIKSMDQIIDGIQQVLDKSENKWNVEKVRHDTLSDQYMTLLDQERNYY
KLTKDLKKNVERRIIN*kikxlq*ink*i

Frame C:
f**qsnirflildtwctfvfgwillfiyfnhhfyl*yisflshkthtkhtltkththt*i
ntq**kkkengekkeyifck***r*yiirrfk*fyktwynytr*dckct*rrvitnd*c*
igteisrny*tfsyrwlf*styfrfiti**scwwhglvnhcfkc*c*r*filsrkfky*t
ksiiirrin*siksyemsi---

---khsmnvslerqrnwmhwkhqrilkhykhyvhwyy*mkh*llkrnfsnnlvnvkw*ni
nkrsnnsyhkmpllkrifktvidnnkfnkhsivilvn*rn*nyysikrieisl*fnvism
nyhlvpnyynikdnllnypnkvvrn*pkpdntiqptiplkirkhf*kmnsqfsiqfnpni
a*lclqqiiknyslnqwikslmvfnkf*inlkingmlkkfvmilylini*ly*ikreiii
n*qkt*rrm*keellikklxnynk*ink
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB221 (VHB221Q) /CSM/VH/VHB2-A/VHB221Q.Seq.d/                       2187   0.0
VHA391 (VHA391Q) /CSM/VH/VHA3-D/VHA391Q.Seq.d/                       1467   0.0
SFC233 (SFC233Q) /CSM/SF/SFC2-B/SFC233Q.Seq.d/                       1429   0.0
VHO250 (VHO250Q) /CSM/VH/VHO2-C/VHO250Q.Seq.d/                       1326   0.0
VFH882 (VFH882Q) /CSM/VF/VFH8-D/VFH882Q.Seq.d/                       1285   0.0
VHB531 (VHB531Q) /CSM/VH/VHB5-B/VHB531Q.Seq.d/                       1176   0.0
VHJ120 (VHJ120Q) /CSM/VH/VHJ1-A/VHJ120Q.Seq.d/                        878   0.0
VHH305 (VHH305Q) /CSM/VH/VHH3-A/VHH305Q.Seq.d/                        741   0.0
VHN779 (VHN779Q) /CSM/VH/VHN7-D/VHN779Q.Seq.d/                        656   0.0
VSH550 (VSH550Q) /CSM/VS/VSH5-C/VSH550Q.Seq.d/                        636   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

DT607214|DT607214.1 ACAG-aaa53a08.g1 Hydra_EST_UCI-9 Hydra magnipapillata cDNA 5'mRNA sequence.    48   1e-05  2
DN604085| unnamed protein product [Mus musculus], mRNA sequence.    48   1e-05  2
EH632656|EH632656.1 EST3763 LK04 Laupala kohalensis cDNA clone 1061021785633 5', mRNA sequence.    50   5e-05  2
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    36   0.043  13
CE632682|CE632682.1 tigr-gss-dog-17000366722551 Dog Library Canis familiaris genomic, genomic survey sequence.    52   0.043  1
AC092430|AC092430.1 Homo sapiens chromosome 2 clone RP11-71H2, WORKING DRAFT SEQUENCE, 12 unordered pieces.    34   0.17   11
EJ122536|EJ122536.1 1092343379815 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001408531 5', genomic survey sequence.    50   0.17   1
CR962127|CR962127.1 Medicago truncatula chromosome 5 clone mth2-181n9, COMPLETE  SEQUENCE.    50   0.17   1
CR759958|CR759958.7 Zebrafish DNA sequence from clone DKEY-50G2 in linkage group 17.    50   0.17   1
CR388413|CR388413.16 Zebrafish DNA sequence from clone DKEY-270B7 in linkage group 4.    42   0.38   4
dna update 2007. 3.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54CV3) RecName: Full=Coiled-coil domain-containing protein 93 ...   397   e-109
BC115269_1(BC115269|pid:none) Danio rerio coiled-coil domain con...   167   6e-40
AM393188_1(AM393188|pid:none) Synthetic construct Homo sapiens c...   164   9e-39
AB384355_1(AB384355|pid:none) Synthetic construct DNA, clone: pF...   164   9e-39
BC134535_1(BC134535|pid:none) Bos taurus hypothetical LOC616839,...   163   1e-38
(Q640U7) RecName: Full=Coiled-coil domain-containing protein 93;...   163   2e-38
AK159415_1(AK159415|pid:none) Mus musculus osteoclast-like cell ...   163   2e-38
AK085701_1(AK085701|pid:none) Mus musculus 10 days lactation, ad...   163   2e-38
BC024674_1(BC024674|pid:none) Mus musculus coiled-coil domain co...   163   2e-38
(Q5BJT7) RecName: Full=Coiled-coil domain-containing protein 93;...   162   4e-38
protein update 2009. 6.27
PSORT 

 psg: 0.58  gvh: 0.30  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.03
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB221 is nuc
5' end seq. ID VHB221F
5' end seq. 
>VHB221F.Seq
CATTTTAGTAACAATCAAATATAAGATTTCTTATATTGGATACGTGGTGTACTTTTGTGT
TTGGGTGGATTTTATTATTTATTTATTTTAATCATCATTTTTATTTGTAATATATTTCAT
TTTTATCACACAAAACACACACAAAACACACACTCACAAAAACACACACTCACACATAAA
TAAATACACAATAATAAAAGAAGAAAGAAAATGGAGAAAAGAAAGAGTATATTTTTTGCA
AATGATAGTGAAGATAATACATCATCAGGAGATTTAAGTAGTTTTACAAAACGTGGTACA
ATTACACCAGGTAAGATTGCAAATGCACGTGAAGGAGAGTTATCACAAATGATTGATGTT
GAATCGGAACAGAAATATCAAGAAATTATTGAACTTTTAGTTACAGGTGGTTATTTTAGA
GCACGTATTTCAGGTTTATCACCATTTGATAAAGTTGTTGGTGGCATGGCTTGGTCAATC
ACTGCTTCAAATGTTGATGTTGACGCTGATTTATTCTTTCAAGAAAATTCAAATATTAAA
CAAAAAGTATCATTATCAGAAGAATTAATTAAAGCATTAAATCGTATGAAATGTCCATTT
NNNNNNNNNN
Length of 5' end seq. 610
3' end seq. ID VHB221Z
3' end seq. 
>VHB221Z.Seq
NNNNNNNNNNACAAGCATTCAATGAACGTATCATTAGAGAGACAGAGAAATTGGATGCAT
TGGAAACACCAGAGAATACTAAAGCATTACAAGCACTACGTTCATTGGTACTATTGAATG
AAACATTGATTGCTCAAGAGGAACTTTTCAAACAATCTTGTAAACGTCAAATGGTAGAAT
ATAAACAAAAGATCGAACAACTCTTATCACAAGATGCCTCTACTCAAGAGGATCTTCAAG
ACAGTGATAGACAACAACAAATTCAACAAGCATTCGATAGTGATATTGGTAAATTAAAGA
AATTGAAATTATTACTCAATAAAAAGAATAGAGATATCTCTTTGATTCAACGTTATCTCG
ATGAATTACCATCTCGTGCCGAACTATTACAATATCAAAGACAATTTGTTGAATTATCCG
AACAAAGTAGTTCGAAATTAACCGAAACCAGACAATACTATACAACCTACAATACCTTTG
AAGATAAGAAAACACTTTTAGAAAATGAACTCTCAATTCTCAATTCAATTCAATCCAAAT
ATAGCATAGCTATGTCTTCAACAAATAATAAAGAATTATTCATTAAATCAATGGATCAAA
TCATTGATGGTATTCAACAAGTTTTAGATAAATCTGAAAATAAATGGAATGTTGAAAAAG
TTCGTCATGATACTTTATCTGATCAATATATGACTTTATTAGATCAAGAGAGAAATTATT
ATAAATTAACAAAAGACTTGAAGAAGAATGTAGAAAGAAGAATTATTAATTAAAAAATTA
AANAATTACAATAAATAAATAAATAAATA
Length of 3' end seq. 809
Connected seq. ID VHB221P
Connected seq. 
>VHB221P.Seq
CATTTTAGTAACAATCAAATATAAGATTTCTTATATTGGATACGTGGTGTACTTTTGTGT
TTGGGTGGATTTTATTATTTATTTATTTTAATCATCATTTTTATTTGTAATATATTTCAT
TTTTATCACACAAAACACACACAAAACACACACTCACAAAAACACACACTCACACATAAA
TAAATACACAATAATAAAAGAAGAAAGAAAATGGAGAAAAGAAAGAGTATATTTTTTGCA
AATGATAGTGAAGATAATACATCATCAGGAGATTTAAGTAGTTTTACAAAACGTGGTACA
ATTACACCAGGTAAGATTGCAAATGCACGTGAAGGAGAGTTATCACAAATGATTGATGTT
GAATCGGAACAGAAATATCAAGAAATTATTGAACTTTTAGTTACAGGTGGTTATTTTAGA
GCACGTATTTCAGGTTTATCACCATTTGATAAAGTTGTTGGTGGCATGGCTTGGTCAATC
ACTGCTTCAAATGTTGATGTTGACGCTGATTTATTCTTTCAAGAAAATTCAAATATTAAA
CAAAAAGTATCATTATCAGAAGAATTAATTAAAGCATTAAATCGTATGAAATGTCCATTT
----------ACAAGCATTCAATGAACGTATCATTAGAGAGACAGAGAAATTGGATGCAT
TGGAAACACCAGAGAATACTAAAGCATTACAAGCACTACGTTCATTGGTACTATTGAATG
AAACATTGATTGCTCAAGAGGAACTTTTCAAACAATCTTGTAAACGTCAAATGGTAGAAT
ATAAACAAAAGATCGAACAACTCTTATCACAAGATGCCTCTACTCAAGAGGATCTTCAAG
ACAGTGATAGACAACAACAAATTCAACAAGCATTCGATAGTGATATTGGTAAATTAAAGA
AATTGAAATTATTACTCAATAAAAAGAATAGAGATATCTCTTTGATTCAACGTTATCTCG
ATGAATTACCATCTCGTGCCGAACTATTACAATATCAAAGACAATTTGTTGAATTATCCG
AACAAAGTAGTTCGAAATTAACCGAAACCAGACAATACTATACAACCTACAATACCTTTG
AAGATAAGAAAACACTTTTAGAAAATGAACTCTCAATTCTCAATTCAATTCAATCCAAAT
ATAGCATAGCTATGTCTTCAACAAATAATAAAGAATTATTCATTAAATCAATGGATCAAA
TCATTGATGGTATTCAACAAGTTTTAGATAAATCTGAAAATAAATGGAATGTTGAAAAAG
TTCGTCATGATACTTTATCTGATCAATATATGACTTTATTAGATCAAGAGAGAAATTATT
ATAAATTAACAAAAGACTTGAAGAAGAATGTAGAAAGAAGAATTATTAATTAAAAAATTA
AANAATTACAATAAATAAATAAATAAATA
Length of connected seq. 1399
Full length Seq ID -
Full length Seq. -
Length of full length seq. -