>VHB205 (VHB205Q) /CSM/VH/VHB2-A/VHB205Q.Seq.d/
ATTTAGAATATTTTAGATTAAAAAACAAAATGAGAGTTTTGTTACTTTTAGTTTTCCTCG
TATTTTGTATCATTCCAGCAGCTCTCTCATTCAGAGACACCAACTGGAGCAAATACAACA
GAGCTGGTAAAAAAGAAACTGTAGATTTCCGTATTGCACTCAATCAAAGAAATTTAGATG
TCTTAGAAAATACTTTATTGGATGTTTCTAACCCAGAATCACCAAATTATGGTAAACATT
GGACTTCTGAACAAGTTGCTGATTTAGTTGCACCAGAACCATACGTTTCAAAGAAAGTTG
CAAATTATTTAAAAAAGAATGGTTGTCACAATGTTGAAAATCATCGTGATTACATTAAAG
CAACTGGTCCAGTTGATTCAGTTGAAAAGATTTTCTCAGTTGAAATGTACAATTACAAAC
ATAATATTAAATCCGATCAAGCTATCATTCGTTCATCAGAAACCTATCAAGTTCCATTTG
AAATCTCTCAATATGTTCACATTGTTTCTGGTATCTCTGAACTCCCACATCTCAAGAGTG
GTCCAAAATCACAAAGATTAAGACCAAATCCAAAGGATTTCCAAGCTACTCCAGCCCCAG
CTGGTCCAGCTGATCCAGGTATGGTCGTTCCACAAACCATCCAACXXXXXXXXXXTCAGG
TGATCAAGGTGCCCCAGGTGATGGTAATCCATCATGTACCTCAAGAGATCAACCAATCTC
AACCATTTTCCCAGGTGCCTCCCCATGGGTTACCTCTGTTGGTGCTACCATGTTAGCTGC
CCCATCCACCAGCAATCCAAATCCAATCGATACTTCTGATGCCCCAGTCTGTGGTATCCA
CGCTTGTGCTACCTCAACTCAAGAAGTTACCTGTTCATCTCCATCCGCTTTGATCACTAC
TGGTGGTGGTTTCTCCAACTACTCCCCACAACCAGCATGGCAATCAAAAGCTGTCAATGC
TTACCTCAACTCTGGTGTTGTCTTACCAAACAAAAAATTCTTTAACGCTTCAAACCGTGG
TTTCCCAGATGTCTCTGGTTTAGGTCACAACTACTACATTGTTGCCGCCGATCAAATGAA
CTTAGTCGATGGTACCTCTTGTTCATCACCAGTCATTGGTGGTATTGTTGCCTTATTAAA
CAGCTATCGTTTAGATAATGGTAAATCAACCCTCGGTTTCATTAACCCAGTCTTATATGC
TGCTTACGCCTCTAACCCAGCTGCCTTCAATGATATCACCGTTGGTAACAATTATTGTAC
TGAAAGCTGTTGTGCTACCACTGGTTTCGTTGCCACCAAAGGTTGGGATGCTGTCACTGG
TTTAGGTTCACCAAATTTCAAACCATTA

LEYFRLKNKMRVLLLLVFLVFCIIPAALSFRDTNWSKYNRAGKKETVDFRIALNQRNLDV
LENTLLDVSNPESPNYGKHWTSEQVADLVAPEPYVSKKVANYLKKNGCHNVENHRDYIKA
TGPVDSVEKIFSVEMYNYKHNIKSDQAIIRSSETYQVPFEISQYVHIVSGISELPHLKSG
PKSQRLRPNPKDFQATPAPAGPADPGMVVPQTIQ---

---SGDQGAPGDGNPSCTSRDQPISTIFPGASPWVTSVGATMLAAPSTSNPNPIDTSDAP
VCGIHACATSTQEVTCSSPSALITTGGGFSNYSPQPAWQSKAVNAYLNSGVVLPNKKFFN
ASNRGFPDVSGLGHNYYIVAADQMNLVDGTSCSSPVIGGIVALLNSYRLDNGKSTLGFIN
PVLYAAYASNPAAFNDITVGNNYCTESCCATTGFVATKGWDAVTGLGSPNFKPL

Frame A:
i*nild*ktk*efcyf*fssyfvsfqqlshsetptganttelvkkkl*isvlhsikei*m
s*kilywmfltqnhqimvnigllnklli*lhqnhtfqrklqii*krmvvtmlkiivitlk
qlvqliqlkrfsqlkctitniilnpiklsfvhqkpikfhlkslnmftlflvslnshisrv
vqnhkd*dqiqriskllqpqlvqliqvwsfhkpsn---

---SGDQGAPGDGNPSCTSRDQPISTIFPGASPWVTSVGATMLAAPSTSNPNPIDTSDAP
VCGIHACATSTQEVTCSSPSALITTGGGFSNYSPQPAWQSKAVNAYLNSGVVLPNKKFFN
ASNRGFPDVSGLGHNYYIVAADQMNLVDGTSCSSPVIGGIVALLNSYRLDNGKSTLGFIN
PVLYAAYASNPAAFNDITVGNNYCTESCCATTGFVATKGWDAVTGLGSPNFKPL

Frame B:
frif*ikkqnesfvtfsfprilyhssssliqrhqleqiqqsw*krncrfpyctqskkfrc
lrkyfigcf*pritklw*tldf*tsc*fsctrtirfkescklfkkewlsqc*kss*lh*s
nwss*fs*kdfls*nvqlqt*y*irssyhsfirnlsssi*nlsicshcfwyl*tptsqew
skitkiktkskgfpsysspswss*srygrstnhp---

---qvikvpqvmvihhvpqeinqsqpfsqvpphglpllvlpc*lphppaiqiqsillmpq
svvstlvlpqlkklpvhlhpl*sllvvvspttphnqhgnqklsmltstlvlsyqtknslt
lqtvvsqmslv*vttttllppik*t*smvplvhhqslvvllpy*taiv*imvnqpsvslt
qsymlltpltqlpsmisplvtiivlkavvlplvslppkvgmlslv*vhqisnh

Frame C:
LEYFRLKNKMRVLLLLVFLVFCIIPAALSFRDTNWSKYNRAGKKETVDFRIALNQRNLDV
LENTLLDVSNPESPNYGKHWTSEQVADLVAPEPYVSKKVANYLKKNGCHNVENHRDYIKA
TGPVDSVEKIFSVEMYNYKHNIKSDQAIIRSSETYQVPFEISQYVHIVSGISELPHLKSG
PKSQRLRPNPKDFQATPAPAGPADPGMVVPQTIQ---

---r*srcpr*w*simylkrstnlnhfprclpmgylcwcyhvscpihqqsksnryf*cps
lwyprlcylnsrsylfisirfdhywwwflqllpttsmaikscqclpqlwccltkqkil*r
fkpwfprclwfrsqllhccrrsnelsrwyllfitshwwycclikqlsfr*w*inprfh*p
slicclrl*psclq*yhrw*qlly*kllcyhwfrchqrlgcchwfrftkfqti

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB205 (VHB205Q) /CSM/VH/VHB2-A/VHB205Q.Seq.d/                       2613   0.0
VSD867 (VSD867Q) /CSM/VS/VSD8-C/VSD867Q.Seq.d/                       1374   0.0
VHK260 (VHK260Q) /CSM/VH/VHK2-C/VHK260Q.Seq.d/                       1374   0.0
VHH845 (VHH845Q) /CSM/VH/VHH8-B/VHH845Q.Seq.d/                       1374   0.0
FC-BB24 (FC-BB24Q) /CSM/FC/FC-BB/FC-BB24Q.Seq.d/                     1374   0.0
VHB560 (VHB560Q) /CSM/VH/VHB5-C/VHB560Q.Seq.d/                       1368   0.0
VHQ391 (VHQ391Q) /CSM/VH/VHQ3-D/VHQ391Q.Seq.d/                       1366   0.0
VHA629 (VHA629Q) /CSM/VH/VHA6-B/VHA629Q.Seq.d/                       1364   0.0
VHB640 (VHB640Q) /CSM/VH/VHB6-B/VHB640Q.Seq.d/                       1360   0.0
VHH776 (VHH776Q) /CSM/VH/VHH7-D/VHH776Q.Seq.d/                       1326   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EC761285|EC761285.1 PSE00007347 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    66   6e-35  6
EC762663|EC762663.1 PSE00007726 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    66   3e-22  3
EC761294|EC761294.1 PSE00003790 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    66   5e-12  2
EC130556|EC130556.1 HVE00010321 Hartmannella vermiformis Normalized long fraction Hartmannella vermiformis cDNA, mRNA sequence.    46   9e-09  3
EC854298|EC854298.1 HDE00000178 Hyperamoeba dachnaya Non-normalized (Amplicon Express) Hyperamoeba dachnaya cDNA, mRNA sequence.    48   5e-08  3
EC854617|EC854617.1 HDE00000592 Hyperamoeba dachnaya Non-normalized (Amplicon Express) Hyperamoeba dachnaya cDNA, mRNA sequence.    48   6e-08  3
U27540|U27540.1 Dictyostelium discoideum vegetative stage specific V4-7 mRNA, partial cds.    40   2e-04  4
CZ858702|CZ858702.1 OC__Ba0250N03.f OC__Ba Oryza coarctata genomic clone OC__Ba0250N03 5', genomic survey sequence.    38   0.039  3
CZ712823|CZ712823.1 OC__Ba0035D04.r OC__Ba Oryza coarctata genomic clone OC__Ba0035D04 3', genomic survey sequence.    38   0.039  3
EJ786387|EJ786387.1 1093017318380 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061002743994 5', genomic survey sequence.    36   0.27   3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8MZS4) RecName: Full=Physarolisin;          EC=3.4.21.103; Alt...   285   3e-75
DQ242648_1(DQ242648|pid:none) Phanerochaete chrysosporium family...   157   1e-36
BC135587_1(BC135587|pid:none) Xenopus tropicalis hypothetical pr...   142   4e-32
AC116960_13(AC116960|pid:none) Dictyostelium discoideum chromoso...   135   3e-30
BC068900_1(BC068900|pid:none) Xenopus laevis hypothetical protei...   134   6e-30
(Q9EQV6) RecName: Full=Tripeptidyl-peptidase 1;          Short=T...   134   7e-30
DQ100344_1(DQ100344|pid:none) Canis familiaris breed Tibetan Ter...   134   7e-30
(Q9XSB8) RecName: Full=Tripeptidyl-peptidase 1;          Short=T...   134   1e-29
AY268890_1(AY268890|pid:none) Homo sapiens growth-inhibiting pro...   133   1e-29
AF017456_1(AF017456|pid:none) Homo sapiens lysosomal pepstatin i...   133   1e-29

 psg: 1.00  gvh: 0.75  alm: 0.42  top: 0.47  tms: 0.00  mit: 0.30  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: extracellular, including cell wall
	 24.0 %: mitochondrial
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	 12.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for VHB205 is exc

>VHB205F.Seq
ATTTAGAATATTTTAGATTAAAAAACAAAATGAGAGTTTTGTTACTTTTAGTTTTCCTCG
TATTTTGTATCATTCCAGCAGCTCTCTCATTCAGAGACACCAACTGGAGCAAATACAACA
GAGCTGGTAAAAAAGAAACTGTAGATTTCCGTATTGCACTCAATCAAAGAAATTTAGATG
TCTTAGAAAATACTTTATTGGATGTTTCTAACCCAGAATCACCAAATTATGGTAAACATT
GGACTTCTGAACAAGTTGCTGATTTAGTTGCACCAGAACCATACGTTTCAAAGAAAGTTG
CAAATTATTTAAAAAAGAATGGTTGTCACAATGTTGAAAATCATCGTGATTACATTAAAG
CAACTGGTCCAGTTGATTCAGTTGAAAAGATTTTCTCAGTTGAAATGTACAATTACAAAC
ATAATATTAAATCCGATCAAGCTATCATTCGTTCATCAGAAACCTATCAAGTTCCATTTG
AAATCTCTCAATATGTTCACATTGTTTCTGGTATCTCTGAACTCCCACATCTCAAGAGTG
GTCCAAAATCACAAAGATTAAGACCAAATCCAAAGGATTTCCAAGCTACTCCAGCCCCAG
CTGGTCCAGCTGATCCAGGTATGGTCGTTCCACAAACCATCCAACNNNNNNNNNN

>VHB205Z.Seq
NNNNNNNNNNTCAGGTGATCAAGGTGCCCCAGGTGATGGTAATCCATCATGTACCTCAAG
AGATCAACCAATCTCAACCATTTTCCCAGGTGCCTCCCCATGGGTTACCTCTGTTGGTGC
TACCATGTTAGCTGCCCCATCCACCAGCAATCCAAATCCAATCGATACTTCTGATGCCCC
AGTCTGTGGTATCCACGCTTGTGCTACCTCAACTCAAGAAGTTACCTGTTCATCTCCATC
CGCTTTGATCACTACTGGTGGTGGTTTCTCCAACTACTCCCCACAACCAGCATGGCAATC
AAAAGCTGTCAATGCTTACCTCAACTCTGGTGTTGTCTTACCAAACAAAAAATTCTTTAA
CGCTTCAAACCGTGGTTTCCCAGATGTCTCTGGTTTAGGTCACAACTACTACATTGTTGC
CGCCGATCAAATGAACTTAGTCGATGGTACCTCTTGTTCATCACCAGTCATTGGTGGTAT
TGTTGCCTTATTAAACAGCTATCGTTTAGATAATGGTAAATCAACCCTCGGTTTCATTAA
CCCAGTCTTATATGCTGCTTACGCCTCTAACCCAGCTGCCTTCAATGATATCACCGTTGG
TAACAATTATTGTACTGAAAGCTGTTGTGCTACCACTGGTTTCGTTGCCACCAAAGGTTG
GGATGCTGTCACTGGTTTAGGTTCACCAAATTTCAAACCATTA

>VHB205P.Seq
ATTTAGAATATTTTAGATTAAAAAACAAAATGAGAGTTTTGTTACTTTTAGTTTTCCTCG
TATTTTGTATCATTCCAGCAGCTCTCTCATTCAGAGACACCAACTGGAGCAAATACAACA
GAGCTGGTAAAAAAGAAACTGTAGATTTCCGTATTGCACTCAATCAAAGAAATTTAGATG
TCTTAGAAAATACTTTATTGGATGTTTCTAACCCAGAATCACCAAATTATGGTAAACATT
GGACTTCTGAACAAGTTGCTGATTTAGTTGCACCAGAACCATACGTTTCAAAGAAAGTTG
CAAATTATTTAAAAAAGAATGGTTGTCACAATGTTGAAAATCATCGTGATTACATTAAAG
CAACTGGTCCAGTTGATTCAGTTGAAAAGATTTTCTCAGTTGAAATGTACAATTACAAAC
ATAATATTAAATCCGATCAAGCTATCATTCGTTCATCAGAAACCTATCAAGTTCCATTTG
AAATCTCTCAATATGTTCACATTGTTTCTGGTATCTCTGAACTCCCACATCTCAAGAGTG
GTCCAAAATCACAAAGATTAAGACCAAATCCAAAGGATTTCCAAGCTACTCCAGCCCCAG
CTGGTCCAGCTGATCCAGGTATGGTCGTTCCACAAACCATCCAAC----------TCAGG
TGATCAAGGTGCCCCAGGTGATGGTAATCCATCATGTACCTCAAGAGATCAACCAATCTC
AACCATTTTCCCAGGTGCCTCCCCATGGGTTACCTCTGTTGGTGCTACCATGTTAGCTGC
CCCATCCACCAGCAATCCAAATCCAATCGATACTTCTGATGCCCCAGTCTGTGGTATCCA
CGCTTGTGCTACCTCAACTCAAGAAGTTACCTGTTCATCTCCATCCGCTTTGATCACTAC
TGGTGGTGGTTTCTCCAACTACTCCCCACAACCAGCATGGCAATCAAAAGCTGTCAATGC
TTACCTCAACTCTGGTGTTGTCTTACCAAACAAAAAATTCTTTAACGCTTCAAACCGTGG
TTTCCCAGATGTCTCTGGTTTAGGTCACAACTACTACATTGTTGCCGCCGATCAAATGAA
CTTAGTCGATGGTACCTCTTGTTCATCACCAGTCATTGGTGGTATTGTTGCCTTATTAAA
CAGCTATCGTTTAGATAATGGTAAATCAACCCTCGGTTTCATTAACCCAGTCTTATATGC
TGCTTACGCCTCTAACCCAGCTGCCTTCAATGATATCACCGTTGGTAACAATTATTGTAC
TGAAAGCTGTTGTGCTACCACTGGTTTCGTTGCCACCAAAGGTTGGGATGCTGTCACTGG
TTTAGGTTCACCAAATTTCAAACCATTA