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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB178
Library VH
(Link to library)
Clone ID VHB178
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHB178P
(Link to Original site)
Representative DNA sequence 
>VHB178 (VHB178Q) /CSM/VH/VHB1-D/VHB178Q.Seq.d/
CTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGC
CATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGG
TAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTC
TGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAA
ATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTG
TACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCATTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGATAAGGAATTCGGTGATGTCACTGTATCCCCAGXXXXXXXXXXTNGTAAAGGTATTGT
TATTGGTGAANAACGTTGNATCGGTTCCCCATTATTTTNTGTCAAAGNTCATTTACCAGT
ATTANAATCCTTCGGTTTCNCCGNTGATTTACGTTNCCNCACTGCCGGTCAAGCTTTCCC
NCAATGNGTTTTCGATCATTGGGNTTCAATTGGTGTTGTCAACAAANATAANAAAGCCNC
TGAAGTNGCTTTAGCCNCCANAAAAANAAAAGGTTTAGCCCCANAAATCCCANATNTTGA
CAAATTCCNCGAAAAACTNTAAACNATTTAAGTCGCCCTTTGAGTTTTC
sequence update 2002.10.25
Translated Amino Acid sequence 
SSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKVS
GDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFSS
EVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDHFLLELQLNTEE
AYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---XKGIVIGEXRXIGSPLFXVKXHLPVLXSFGFXXDLRXXTAGQAFPQXVFDHWXSIGV
VNKXXKAXEVALAXXKXKGLAPXIPXXDKFXEKL*ti*val*vf
Translated Amino Acid sequence (All Frames) 
Frame A:
leckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*sf
w*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fqf
rsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*r
slslfp*sy*irqchcw*h*r*gir*chcip---

---x*rycyw*xtlxrfpiixcqxsftsixilrfxr*ftxphcrssfpxmxfrslgfnwc
cqqx*xsx*sxfsxxkxkrfspxnpxx*qiprktxnxlsrplsf

Frame B:
SSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKVS
GDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFSS
EVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDHFLLELQLNTEE
AYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---XKGIVIGEXRXIGSPLFXVKXHLPVLXSFGFXXDLRXXTAGQAFPQXVFDHWXSIGV
VNKXXKAXEVALAXXKXKGLAPXIPXXDKFXEKL*ti*val*vf

Frame C:
rvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpikfl
vi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlisvq
k*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksiisy*nsn*tlkk
lislsvellnpsmsllvtlkirnsvmslypq---

---vkvlllvxnvxsvphyfxskxiyqyxnpsvspxiyvxxlpvklsxnxfsiigxqlvl
stxixkplkxl*ppxkxkv*pxksxxltnsxknxkxfkspfef
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB178 (VHB178Q) /CSM/VH/VHB1-D/VHB178Q.Seq.d/                       1576   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1219   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1219   0.0
VHQ438 (VHQ438Q) /CSM/VH/VHQ4-B/VHQ438Q.Seq.d/                       1219   0.0
VHQ395 (VHQ395Q) /CSM/VH/VHQ3-D/VHQ395Q.Seq.d/                       1219   0.0
VHQ369 (VHQ369Q) /CSM/VH/VHQ3-C/VHQ369Q.Seq.d/                       1219   0.0
VHQ311 (VHQ311Q) /CSM/VH/VHQ3-A/VHQ311Q.Seq.d/                       1219   0.0
VHQ288 (VHQ288Q) /CSM/VH/VHQ2-D/VHQ288Q.Seq.d/                       1219   0.0
VHQ248 (VHQ248Q) /CSM/VH/VHQ2-B/VHQ248Q.Seq.d/                       1219   0.0
VHQ212 (VHQ212Q) /CSM/VH/VHQ2-A/VHQ212Q.Seq.d/                       1219   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1215   0.0    1
DY889811|DY889811.1 CeleSEQ6761 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6761, mRNA sequence.    84   3e-32  4
DY889772|DY889772.1 CeleSEQ11980 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ11980, mRNA sequence.    84   3e-32  4
DY890935|DY890935.1 CeleSEQ7425 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ7425, mRNA sequence.    84   5e-32  4
DY887441|DY887441.1 CeleSEQ3766 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ3766, mRNA sequence.    84   5e-32  4
CX155337|CX155337.1 TfEST072F03 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    84   7e-32  4
DY888936|DY888936.1 CeleSEQ6366 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6366, mRNA sequence.    84   7e-32  4
CX154324|CX154324.1 TfEST033C12 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    84   1e-31  4
CX154971|CX154971.1 TfEST089A07 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    84   3e-31  4
CX154356|CX154356.1 TfEST029E10 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    84   4e-31  4
dna update 2007. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   271   3e-71
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   266   1e-69
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   265   2e-69
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   264   4e-69
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   264   4e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   264   4e-69
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   263   5e-69
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   263   6e-69
AB095183_1(AB095183|pid:none) Cyanidioschyzon merolae gene for e...   261   2e-68
CU638744_366(CU638744|pid:none) Podospora anserina genomic DNA c...   261   2e-68
protein update 2009. 6.27
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHB178 is cyt
5' end seq. ID VHB178F
5' end seq. 
>VHB178F.Seq
CTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGC
CATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGG
TAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTC
TGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAA
ATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTG
TACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCATTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGATAAGGAATTCGGTGATGTCACTGTATCCCCAGNNNNNNNNNN
Length of 5' end seq. 645
3' end seq. ID VHB178Z
3' end seq. 
>VHB178Z.Seq
NNNNNNNNNNTNGTAAAGGTATTGTTATTGGTGAANAACGTTGNATCGGTTCCCCATTAT
TTTNTGTCAAAGNTCATTTACCAGTATTANAATCCTTCGGTTTCNCCGNTGATTTACGTT
NCCNCACTGCCGGTCAAGCTTTCCCNCAATGNGTTTTCGATCATTGGGNTTCAATTGGTG
TTGTCAACAAANATAANAAAGCCNCTGAAGTNGCTTTAGCCNCCANAAAAANAAAAGGTT
TAGCCCCANAAATCCCANATNTTGACAAATTCCNCGAAAAACTNTAAACNATTTAAGTCG
CCCTTTGAGTTTTC
Length of 3' end seq. 314
Connected seq. ID VHB178P
Connected seq. 
>VHB178P.Seq
CTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGC
CATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGG
TAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTC
TGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAA
ATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTG
TACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCATTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGATAAGGAATTCGGTGATGTCACTGTATCCCCAG----------TNGTAAAGGTATTGT
TATTGGTGAANAACGTTGNATCGGTTCCCCATTATTTTNTGTCAAAGNTCATTTACCAGT
ATTANAATCCTTCGGTTTCNCCGNTGATTTACGTTNCCNCACTGCCGGTCAAGCTTTCCC
NCAATGNGTTTTCGATCATTGGGNTTCAATTGGTGTTGTCAACAAANATAANAAAGCCNC
TGAAGTNGCTTTAGCCNCCANAAAAANAAAAGGTTTAGCCCCANAAATCCCANATNTTGA
CAAATTCCNCGAAAAACTNTAAACNATTTAAGTCGCCCTTTGAGTTTTC
Length of connected seq. 939
Full length Seq ID -
Full length Seq. -
Length of full length seq. -