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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB170
Library VH
(Link to library)
Clone ID VHB170
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16315-1
Original site URL
Representative seq. ID VHB170P
(Link to Original site)
Representative DNA sequence 
>VHB170 (VHB170Q) /CSM/VH/VHB1-C/VHB170Q.Seq.d/
CTGCGGAATTCCTCGAGNCACTAGCATNCGAGTCGGCCTTGTTGGCAAAATAATAAAAAA
CTGTTATCACAAAAGTTATATTATATTTTTATTTATTTCAATACCTTTGTATAAGCACTC
CATATATATATATATAATACATAATCATATTTGTAATTAGTGCTATTATTATTACTATCG
AAATAGTTATTCAATTTTTGGGATTTTAAAATTAAAATAAAAAAAAATAACTATAAAAAT
TGTAAAATAGATTAACAAACGCAAANGGAATATTTTAAAAATTAACCTCTTTTTTATTCA
AATATAATAAATCACCCATATATAGGCAAATATGTATAACATGAGTTTTTGGCGTAATTT
TGGATTTCAACCAATATCACATATTGAAAGTATTTTGGATAGAGATTTCTTCACATTAGA
AGACTTATTCGATGAAGATGAACTCTTGCAAGAATGTAAAACACAAAACAAAAGATTAAT
CGAATTTTTAATTAAGCCAGAATCATTAAATGGAATGTTTGAATTTATCACAAAAGAAGX
XXXXXXXXXTCAAATCCATCAAATTTGTCAAATTATTTTATATAGTGAAAATGATGAACT
TAAAATGTCACTTTTAAAGGATGCCAAACTCCTTGATAGAATCATTGAAGCCGATCAAGC
CAACACCAACGAATTAAAACAAGTTAAAGGTATTAGATATGGTTTTATGGGTTTCTTAAA
TAGTATTGCCCAATCTATTTGTGATGTTTCAAAGAATTCACCAGAAATCAAACAAATTGT
TGATGAAAATCCAAAATGGGGAGTATTTGTTACTGAATCGTTAGAACCTGTTTTAAATAT
TGAAAATGAACCATTAGGTGAATTTAAACCAAGTTTAATGACTGCACCAGAAAGTGCTGA
AGAAGATGACGATCAATATGATAATGATGATAATGGTTATGATAACTACGATAGTGTTGG
CGATTATGTTAGTGCCGACCAATATCAAGATCAAATGGATTATAGCGATGAAGATGATTA
TGAAGGTGGTCAAAAACCTATCAATTATATTGAAGAGGAAGATGATGAAGAGGAAGATGA
TGACATACGATTTCAACAACAACAACAACAACAACAACAATTAAATCACCACGACAATCA
AGATGAATTAAATGAACAACCACAACAACCACAACCACAACAAGACGACAAAACTACTCA
AGACACTGAAGCAGTTGTAGAAACTCCAAAAGAATCATCTGTTGACCAAGAATCAACAGA
AGCAAAAACTGAATCTTCATAAACTTTAAAATAATACCAATAACAACAACAAAATCACC
sequence update 2002.10.25
Translated Amino Acid sequence 
cgiprxtsxrvglvgkiikncyhksyiiflfisiplykhsiyiyiihnhicn*cyyyyyr
nsysifgilklk*kkitikivk*inkrkxnilkinlffiqi**ithi*ANMYNMSFWRNF
GFQPISHIESILDRDFFTLEDLFDEDELLQECKTQNKRLIEFLIKPESLNGMFEFITKE-
--

---QIHQICQIILYSENDELKMSLLKDAKLLDRIIEADQANTNELKQVKGIRYGFMGFLN
SIAQSICDVSKNSPEIKQIVDENPKWGVFVTESLEPVLNIENEPLGEFKPSLMTAPESAE
EDDDQYDNDDNGYDNYDSVGDYVSADQYQDQMDYSDEDDYEGGQKPINYIEEEDDEEEDD
DIRFQQQQQQQQQLNHHDNQDELNEQPQQPQPQQDDKTTQDTEAVVETPKESSVDQESTE
AKTESS*tlk*yq*qqqnh
Translated Amino Acid sequence (All Frames) 
Frame A:
lrnssxh*hxsrpcwqnnkkllsqklyyifiyfntfv*alhiyiynt*syl*lvllllls
k*lfnfwdfkikikknnyknckid*qtqxeyfkn*plfysniinhpyigkyv*hefla*f
wistnity*kyfg*rflhirrlir*r*tlarm*ntkqkinrifn*ariikwnv*iyhkr-
--

---snpsnlsnyfi**k**t*nvtfkgcqtp**nh*srssqhqrikts*ry*iwfygflk
*ycpiyl*cfkeftrnqtnc**kskmgsicy*ivrtcfky*k*tir*i*tkfndctrkc*
rr*rsi*****wl**lr*cwrlc*crpisrsngl*r*r*l*rwsktyqly*rgr**rgr*
*htisttttttttiksprqsr*ik*tttttttttrrqnysrh*sscrnskriic*prinr
skn*ifinfkiipitttks

Frame B:
cgiprxtsxrvglvgkiikncyhksyiiflfisiplykhsiyiyiihnhicn*cyyyyyr
nsysifgilklk*kkitikivk*inkrkxnilkinlffiqi**ithi*ANMYNMSFWRNF
GFQPISHIESILDRDFFTLEDLFDEDELLQECKTQNKRLIEFLIKPESLNGMFEFITKE-
--

---QIHQICQIILYSENDELKMSLLKDAKLLDRIIEADQANTNELKQVKGIRYGFMGFLN
SIAQSICDVSKNSPEIKQIVDENPKWGVFVTESLEPVLNIENEPLGEFKPSLMTAPESAE
EDDDQYDNDDNGYDNYDSVGDYVSADQYQDQMDYSDEDDYEGGQKPINYIEEEDDEEEDD
DIRFQQQQQQQQQLNHHDNQDELNEQPQQPQPQQDDKTTQDTEAVVETPKESSVDQESTE
AKTESS*tlk*yq*qqqnh

Frame C:
aeflexlaxesallak**ktvitkvilyfylfqylcistpyiyi*yiiifvisaiiitie
iviqflgf*n*nkkk*l*kl*nrltnaxgif*kltsflfkynkspiyrqicit*vfgvil
dfnqyhilkvfwieissh*ktysmkmnscknvkhktkd*snf*lsqnh*meclnlsqkk-
--

---ksikfvklfyivkmmnlkchf*rmpnslieslkpikptptn*nklkvldmvlwvs*i
vlpnlfvmfqrihqksnkllmkiqngeylllnr*nlf*ilkmnh*vnlnqv**lhqkvlk
kmtinmimmimvmittivlaimlvptnikikwiiamkmimkvvknlsiilkrkmmkrkmm
tydfnnnnnnnnn*itttikmn*mnnhnnhnhnkttkllktlkql*klqknhlltknqqk
qklnlhkl*nntnnnnkit
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB170 (VHB170Q) /CSM/VH/VHB1-C/VHB170Q.Seq.d/                       1612   0.0
VHP518 (VHP518Q) /CSM/VH/VHP5-A/VHP518Q.Seq.d/                       1078   0.0
VHA160 (VHA160Q) /CSM/VH/VHA1-C/VHA160Q.Seq.d/                       1078   0.0
SFE787 (SFE787Q) /CSM/SF/SFE7-D/SFE787Q.Seq.d/                       1078   0.0
AHD563 (AHD563Q) /CSM/AH/AHD5-C/AHD563Q.Seq.d/                       1078   0.0
AHM222 (AHM222Q) /CSM/AH/AHM2-A/AHM222Q.Seq.d/                       1074   0.0
AFL457 (AFL457Q) /CSM/AF/AFL4-C/AFL457Q.Seq.d/                       1072   0.0
VHJ164 (VHJ164Q) /CSM/VH/VHJ1-C/VHJ164Q.Seq.d/                       1070   0.0
VHP592 (VHP592Q) /CSM/VH/VHP5-D/VHP592Q.Seq.d/                       1068   0.0
AHH323 (AHH323Q) /CSM/AH/AHH3-A/AHH323Q.Seq.d/                       1065   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL031745|AL031745.8 Plasmodium falciparum DNA from MAL1P2.         40   9e-06  3
AU087361|AU087361.2 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn4281.    40   1e-05  3
CT663588|CT663588.2 Danio rerio EST, clone ZF_mu_122c05 5'.        48   2e-04  2
CT627387|CT627387.1 Danio rerio EST, clone ZF_mu_66e19 3'.         48   2e-04  2
CN021875|CN021875.1 AGENCOURT_20189303 NIH_ZGC_15 Danio rerio cDNA clone IMAGE:7226395 5', mRNA sequence.    48   2e-04  2
EG583548|EG583548.1 AGENCOURT_88167008 NIH_ZGC_35 Danio rerio cDNA clone IMAGE:8754312 5', mRNA sequence.    48   2e-04  2
CT610992|CT610992.2 Danio rerio EST, clone ZF_mu_123l14 5'.        48   2e-04  2
CK697119|CK697119.1 ZF101-P00075-DEPE-F3_D05 GISZF001_ra Danio rerio cDNA clone IMAGE:7162519 5', mRNA sequence.    48   2e-04  2
CT609043|CT609043.2 Danio rerio EST, clone ZF_mu_176n03 5'.        48   2e-04  2
DT059453|DT059453.1 AGENCOURT_56130024 NIH_ZGC_10 Danio rerio cDNA clone IMAGE:8004417 5', mRNA sequence.    48   2e-04  2
dna update 2007. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE017347_191(AE017347|pid:none) Cryptococcus neoformans var. neo...    50   2e-04
(O74511) RecName: Full=Extragenic suppressor of kinetochore prot...    50   2e-04
AL031532_17(AL031532|pid:none) S.pombe chromosome III cosmid c777.     50   2e-04
(Q6NRI0) RecName: Full=Serine/threonine-protein phosphatase 6 re...    46   0.003
BC121957_1(BC121957|pid:none) Xenopus tropicalis hypothetical pr...    46   0.003
(Q5F471) RecName: Full=Serine/threonine-protein phosphatase 6 re...    44   0.010
CR749815_1(CR749815|pid:none) Homo sapiens mRNA; cDNA DKFZp781E1...    44   0.010
(Q922D4) RecName: Full=Serine/threonine-protein phosphatase 6 re...    44   0.010
AF264780_1(AF264780|pid:none) Homo sapiens sporulation-induced t...    44   0.010
AK018652_1(AK018652|pid:none) Mus musculus adult male cecum cDNA...    44   0.010
protein update 2009. 2.23
PSORT 

 psg: 0.80  gvh: 0.33  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VHB170 is nuc
5' end seq. ID VHB170F
5' end seq. 
>VHB170F.Seq
CTGCGGAATTCCTCGAGNCACTAGCATNCGAGTCGGCCTTGTTGGCAAAATAATAAAAAA
CTGTTATCACAAAAGTTATATTATATTTTTATTTATTTCAATACCTTTGTATAAGCACTC
CATATATATATATATAATACATAATCATATTTGTAATTAGTGCTATTATTATTACTATCG
AAATAGTTATTCAATTTTTGGGATTTTAAAATTAAAATAAAAAAAAATAACTATAAAAAT
TGTAAAATAGATTAACAAACGCAAANGGAATATTTTAAAAATTAACCTCTTTTTTATTCA
AATATAATAAATCACCCATATATAGGCAAATATGTATAACATGAGTTTTTGGCGTAATTT
TGGATTTCAACCAATATCACATATTGAAAGTATTTTGGATAGAGATTTCTTCACATTAGA
AGACTTATTCGATGAAGATGAACTCTTGCAAGAATGTAAAACACAAAACAAAAGATTAAT
CGAATTTTTAATTAAGCCAGAATCATTAAATGGAATGTTTGAATTTATCACAAAAGAAGN
NNNNNNNNN
Length of 5' end seq. 549
3' end seq. ID VHB170Z
3' end seq. 
>VHB170Z.Seq
NNNNNNNNNNTCAAATCCATCAAATTTGTCAAATTATTTTATATAGTGAAAATGATGAAC
TTAAAATGTCACTTTTAAAGGATGCCAAACTCCTTGATAGAATCATTGAAGCCGATCAAG
CCAACACCAACGAATTAAAACAAGTTAAAGGTATTAGATATGGTTTTATGGGTTTCTTAA
ATAGTATTGCCCAATCTATTTGTGATGTTTCAAAGAATTCACCAGAAATCAAACAAATTG
TTGATGAAAATCCAAAATGGGGAGTATTTGTTACTGAATCGTTAGAACCTGTTTTAAATA
TTGAAAATGAACCATTAGGTGAATTTAAACCAAGTTTAATGACTGCACCAGAAAGTGCTG
AAGAAGATGACGATCAATATGATAATGATGATAATGGTTATGATAACTACGATAGTGTTG
GCGATTATGTTAGTGCCGACCAATATCAAGATCAAATGGATTATAGCGATGAAGATGATT
ATGAAGGTGGTCAAAAACCTATCAATTATATTGAAGAGGAAGATGATGAAGAGGAAGATG
ATGACATACGATTTCAACAACAACAACAACAACAACAACAATTAAATCACCACGACAATC
AAGATGAATTAAATGAACAACCACAACAACCACAACCACAACAAGACGACAAAACTACTC
AAGACACTGAAGCAGTTGTAGAAACTCCAAAAGAATCATCTGTTGACCAAGAATCAACAG
AAGCAAAAACTGAATCTTCATAAACTTTAAAATAATACCAATAACAACAACAAAATCACC
Length of 3' end seq. 780
Connected seq. ID VHB170P
Connected seq. 
>VHB170P.Seq
CTGCGGAATTCCTCGAGNCACTAGCATNCGAGTCGGCCTTGTTGGCAAAATAATAAAAAA
CTGTTATCACAAAAGTTATATTATATTTTTATTTATTTCAATACCTTTGTATAAGCACTC
CATATATATATATATAATACATAATCATATTTGTAATTAGTGCTATTATTATTACTATCG
AAATAGTTATTCAATTTTTGGGATTTTAAAATTAAAATAAAAAAAAATAACTATAAAAAT
TGTAAAATAGATTAACAAACGCAAANGGAATATTTTAAAAATTAACCTCTTTTTTATTCA
AATATAATAAATCACCCATATATAGGCAAATATGTATAACATGAGTTTTTGGCGTAATTT
TGGATTTCAACCAATATCACATATTGAAAGTATTTTGGATAGAGATTTCTTCACATTAGA
AGACTTATTCGATGAAGATGAACTCTTGCAAGAATGTAAAACACAAAACAAAAGATTAAT
CGAATTTTTAATTAAGCCAGAATCATTAAATGGAATGTTTGAATTTATCACAAAAGAAG-
---------TCAAATCCATCAAATTTGTCAAATTATTTTATATAGTGAAAATGATGAACT
TAAAATGTCACTTTTAAAGGATGCCAAACTCCTTGATAGAATCATTGAAGCCGATCAAGC
CAACACCAACGAATTAAAACAAGTTAAAGGTATTAGATATGGTTTTATGGGTTTCTTAAA
TAGTATTGCCCAATCTATTTGTGATGTTTCAAAGAATTCACCAGAAATCAAACAAATTGT
TGATGAAAATCCAAAATGGGGAGTATTTGTTACTGAATCGTTAGAACCTGTTTTAAATAT
TGAAAATGAACCATTAGGTGAATTTAAACCAAGTTTAATGACTGCACCAGAAAGTGCTGA
AGAAGATGACGATCAATATGATAATGATGATAATGGTTATGATAACTACGATAGTGTTGG
CGATTATGTTAGTGCCGACCAATATCAAGATCAAATGGATTATAGCGATGAAGATGATTA
TGAAGGTGGTCAAAAACCTATCAATTATATTGAAGAGGAAGATGATGAAGAGGAAGATGA
TGACATACGATTTCAACAACAACAACAACAACAACAACAATTAAATCACCACGACAATCA
AGATGAATTAAATGAACAACCACAACAACCACAACCACAACAAGACGACAAAACTACTCA
AGACACTGAAGCAGTTGTAGAAACTCCAAAAGAATCATCTGTTGACCAAGAATCAACAGA
AGCAAAAACTGAATCTTCATAAACTTTAAAATAATACCAATAACAACAACAAAATCACC
Length of connected seq. 1309
Full length Seq ID -
Full length Seq. -
Length of full length seq. -