>VHB152 (VHB152Q) /CSM/VH/VHB1-C/VHB152Q.Seq.d/
ATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTTGAA
AAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCGGATAAT
ATCGTACCAAGTAAATACGATCTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGC
GGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCA
ATTGACATTGAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTAC
TATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAAT
ACAATTCTCTCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGT
TCAAAATATGTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACT
GATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATT
AAAATCACTATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTGTAATT
GAAAATAATGATGGTACTAAAACCTTTATTTTTGAACAAACTCCAAAGATGAGTACTTAT
ATTGTTGCATTTATAGTTGGTGAATTCGATCATATTGAGTCACATACTAAAGAAGXXXXX
XXXXXTCAGATACCCTGTTAAGAGATAAAGTAAACACTAAATTGGGTATGTTGGGTGATG
CTGATATCGTTGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAGACTCTTCAAGTT
TACCATCAGATATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATGGTGGTGAAGCTG
ATCAACAAATCATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTGAAAAGAATTCTT
ACCTTTTGGTACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAGCCCTCAATTTCG
CTTTATCTCCATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTACCCTCCCAAATC
GTGTTCGTCAATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAATCAACGAAACAT
TTAAAAACTCTCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCTCTAAAATGGATG
ATTCTCAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGCTGATC
GTTCAATTAAACAAGATTTAGAAATGGTTACAATTAATTCAAATTGGTTCAAAGCTTTTA
ATCAAGATTTATCAAATTGGATTCAATCAAAATAAAAATAAAATCTCTTACAATGAAAGA
TCTTTGTTTTAATAATNATAGGATAAAAAAAAANAGACGAAAACTAAAATAAACATCAAA
TTAT

IFIISLVYFNFIHINNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVFS
GKVDITINIVKPTKKIIIHSIDIEIQSVKILNQQATSVTYYEPEEVAILEFQDELPVTEN
TILSIDFTGILNDKLKGFYRSKYVVNGEDRYIGTTQFEATDARRAFPCFDEPALKSFFNI
KITISSHLTALSNMDTTSVIENNDGTKTFIFEQTPKMSTYIVAFIVGEFDHIESHTKE--
-

---SDTLLRDKVNTKLGMLGDADIVAECKKRFEAFKADSSSLPSDIRSTVLATIVKNGGE
ADQQIIIDQYLKSSLVAEKNSYLLVLALAPKEELVEKALNFALSPSVRSQDSYMVFFTLP
NRVRQFAWAYLTKNFNQINETFKNSPLFGRIIGSCLTSKMDDSQYNEVVNFFKDHPVPIA
DRSIKQDLEMVTINSNWFKAFNQDLSNWIQSK*k*nllq*kifvlixig*kkxden*nkh
qi

Frame A:
IFIISLVYFNFIHINNNIVEKMCDHKHLVDKVDRLVLPDNIVPSKYDLHLRPNLKDFVFS
GKVDITINIVKPTKKIIIHSIDIEIQSVKILNQQATSVTYYEPEEVAILEFQDELPVTEN
TILSIDFTGILNDKLKGFYRSKYVVNGEDRYIGTTQFEATDARRAFPCFDEPALKSFFNI
KITISSHLTALSNMDTTSVIENNDGTKTFIFEQTPKMSTYIVAFIVGEFDHIESHTKE--
-

---SDTLLRDKVNTKLGMLGDADIVAECKKRFEAFKADSSSLPSDIRSTVLATIVKNGGE
ADQQIIIDQYLKSSLVAEKNSYLLVLALAPKEELVEKALNFALSPSVRSQDSYMVFFTLP
NRVRQFAWAYLTKNFNQINETFKNSPLFGRIIGSCLTSKMDDSQYNEVVNFFKDHPVPIA
DRSIKQDLEMVTINSNWFKAFNQDLSNWIQSK*k*nllq*kifvlixig*kkxden*nkh
qi

Frame B:
fl*fhlfililyi*iii*lkkcviini*ltk*id*yyriisyqvntiyildqi*rilysa
akltlqsillnqpkkslsiqltlkfnq*rs*inkllplptmnqkklqf*nskmnyqlqki
qfsplislvf*mtn*kvsivqnmlsmvkivilvplnskplmlvvlshvlmnll*nlslil
kslfhli*llfqiwiqll*lkimmvlkplflnklqr*vlillhl*lvnsiilshilkk--
-

---qipc*eik*tlnwvcwvmlisllnvrkdsklskqtlqvyhqifvlqyllqs*rmvvk
linksslinilnll*slkriltfwyllwlqkrnwlkkpsislylhplevkiviwfslpsq
ivfvnslgltspktlikstkhlktllclvvslvvvsplkwmilntmksstfskiiqfqll
ivqlnki*kwlqliqigskllikiyqigfnqnknkisynerslf**x*dkkkxtktkini
kl

Frame C:
fynftclf*fytyk**ys*knv*s*tfs*qsr*isitg*yrtk*irsts*tkfkgfciqr
qs*hynqyc*tnqknhypfn*h*nsiskdlkstsyfryll*trrscnfripr*itsyrky
nslh*fhwyfk*qikrflsfkiccqw*rslywyhsirsh*csscfpmf**tcfkifl*y*
nhyfisfncsfkygynfcn*k**wy*nlyf*tnskdeylycciysw*irsy*vty*r---

---rypvkr*skh*igyvg*c*yrc*m*ekirsfqsrlfkftirysfystcynrkeww*s
*stnhh*sis*iffsr*keflpfgtcfgskrgig*kspqfrfisir*ksr*lygflyppk
scssirlglphqkl*snqrni*klsfvwsyhw*lshl*ng*fsiq*srqlfqrssssnc*
sfn*trfrngyn*fklvqsf*srfikldsikikiksltmkdlcfnnxrikkxrrklk*ts
ny

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB152 (VHB152Q) /CSM/VH/VHB1-C/VHB152Q.Seq.d/                       2690   0.0
VHQ305 (VHQ305Q) /CSM/VH/VHQ3-A/VHQ305Q.Seq.d/                       2599   0.0
VHN747 (VHN747Q) /CSM/VH/VHN7-B/VHN747Q.Seq.d/                       1346   0.0
VHB329 (VHB329Q) /CSM/VH/VHB3-B/VHB329Q.Seq.d/                       1346   0.0
AHE191 (AHE191Q) /CSM/AH/AHE1-D/AHE191Q.Seq.d/                       1346   0.0
AHD767 (AHD767Q) /CSM/AH/AHD7-C/AHD767Q.Seq.d/                       1346   0.0
AHM214 (AHM214Q) /CSM/AH/AHM2-A/AHM214Q.Seq.d/                       1344   0.0
AHK544 (AHK544Q) /CSM/AH/AHK5-B/AHK544Q.Seq.d/                       1344   0.0
VHO659 (VHO659Q) /CSM/VH/VHO6-C/VHO659Q.Seq.d/                       1340   0.0
AHH858 (AHH858Q) /CSM/AH/AHH8-C/AHH858Q.Seq.d/                       1340   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY631393|AY631393.1 Dictyostelium discoideum puromycin-sensitive aminopeptidase-like protein (psa) mRNA, partial cds.    80   5e-21  4
CR382136|CR382136.1 Debaryomyces hansenii chromosome D of strain CBS767 of Debaryomyces hansenii.    56   0.003  1
CP000498|CP000498.1 Pichia stipitis CBS 6054 chromosome 4, complete sequence.    38   0.007  2
CR382123|CR382123.1 Kluyveromyces lactis strain NRRL Y-1140 chromosome C of strain NRRL  Y-1140 of Kluyveromyces lactis.    54   0.011  1
DN815134|AF148323_1 aminopeptidase PILS [Rattus norvegicus], mRNA sequence.    52   0.015  2
BP514490|BP514490.1 Hydra magnipapillata cDNA, clone:hydmg018bw_14.    52   0.044  1
AJ808281|AJ808281.1 Antirrhinum majus EST, clone 018_6_09_c24.     50   0.17   1
CV966447|CV966447.1 PC007G7 infected tomato, center of lesion 3 dpi Phytophthora infestans cDNA, mRNA sequence.    36   0.50   2
CB564189|CB564189.1 AGENCOURT_13329805 NICHD_XGC_Tad2 Xenopus laevis cDNA clone IMAGE:6875222 3', mRNA sequence.    48   0.69   1
CL085130|CL085130.1 ISB1-4I13_T7.1 ISB1 Xenopus tropicalis genomic clone ISB1-4I13, genomic survey sequence.    48   0.69   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY631393_1(AY631393|pid:none) Dictyostelium discoideum puromycin...   221   4e-56
AF327435_1(AF327435|pid:none) Drosophila melanogaster puromycin-...   180   1e-43
AE014296_282(AE014296|pid:none) Drosophila melanogaster chromoso...   179   2e-43
AE014296_281(AE014296|pid:none) Drosophila melanogaster chromoso...   179   2e-43
AM270075_67(AM270075|pid:none) Aspergillus niger contig An04c014...   160   1e-37
AP007157_574(AP007157|pid:none) Aspergillus oryzae RIB40 genomic...   159   3e-37
AM431949_6(AM431949|pid:none) Vitis vinifera contig VV78X111726....   158   5e-37
EF678434_1(EF678434|pid:none) Picea sitchensis clone WS02921_A14...   157   1e-36
AF350242_1(AF350242|pid:none) Pichia angusta aminopeptidase gene...   156   2e-36
EF676179_1(EF676179|pid:none) Picea sitchensis clone WS02717_G19...   155   3e-36

 psg: 0.95  gvh: 0.36  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for VHB152 is cyt

>VHB152F.Seq
ATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTTGAA
AAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCGGATAAT
ATCGTACCAAGTAAATACGATCTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGC
GGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCA
ATTGACATTGAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTAC
TATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAAT
ACAATTCTCTCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGT
TCAAAATATGTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACT
GATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATT
AAAATCACTATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTGTAATT
GAAAATAATGATGGTACTAAAACCTTTATTTTTGAACAAACTCCAAAGATGAGTACTTAT
ATTGTTGCATTTATAGTTGGTGAATTCGATCATATTGAGTCACATACTAAAGAAGNNNNN
NNNNN

>VHB152Z.Seq
NNNNNNNNNNTCAGATACCCTGTTAAGAGATAAAGTAAACACTAAATTGGGTATGTTGGG
TGATGCTGATATCGTTGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAGACTCTTC
AAGTTTACCATCAGATATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATGGTGGTGA
AGCTGATCAACAAATCATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTGAAAAGAA
TTCTTACCTTTTGGTACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAGCCCTCAA
TTTCGCTTTATCTCCATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTACCCTCCC
AAATCGTGTTCGTCAATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAATCAACGA
AACATTTAAAAACTCTCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCTCTAAAAT
GGATGATTCTCAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGC
TGATCGTTCAATTAAACAAGATTTAGAAATGGTTACAATTAATTCAAATTGGTTCAAAGC
TTTTAATCAAGATTTATCAAATTGGATTCAATCAAAATAAAAATAAAATCTCTTACAATG
AAAGATCTTTGTTTTAATAATNATAGGATAAAAAAAAANAGACGAAAACTAAAATAAACA
TCAAATTAT

>VHB152P.Seq
ATTTTTATAATTTCACTTGTTTATTTTAATTTTATACATATAAATAATAATATAGTTGAA
AAAATGTGTGATCATAAACATTTAGTTGACAAAGTAGATAGATTAGTATTACCGGATAAT
ATCGTACCAAGTAAATACGATCTACATCTTAGACCAAATTTAAAGGATTTTGTATTCAGC
GGCAAAGTTGACATTACAATCAATATTGTTAAACCAACCAAAAAAATCATTATCCATTCA
ATTGACATTGAAATTCAATCAGTAAAGATCTTAAATCAACAAGCTACTTCCGTTACCTAC
TATGAACCAGAAGAAGTTGCAATTTTAGAATTCCAAGATGAATTACCAGTTACAGAAAAT
ACAATTCTCTCCATTGATTTCACTGGTATTTTAAATGACAAATTAAAAGGTTTCTATCGT
TCAAAATATGTTGTCAATGGTGAAGATCGTTATATTGGTACCACTCAATTCGAAGCCACT
GATGCTCGTCGTGCTTTCCCATGTTTTGATGAACCTGCTTTAAAATCTTTCTTTAATATT
AAAATCACTATTTCATCTCATTTAACTGCTCTTTCAAATATGGATACAACTTCTGTAATT
GAAAATAATGATGGTACTAAAACCTTTATTTTTGAACAAACTCCAAAGATGAGTACTTAT
ATTGTTGCATTTATAGTTGGTGAATTCGATCATATTGAGTCACATACTAAAGAAG-----
-----TCAGATACCCTGTTAAGAGATAAAGTAAACACTAAATTGGGTATGTTGGGTGATG
CTGATATCGTTGCTGAATGTAAGAAAAGATTCGAAGCTTTCAAAGCAGACTCTTCAAGTT
TACCATCAGATATTCGTTCTACAGTACTTGCTACAATCGTAAAGAATGGTGGTGAAGCTG
ATCAACAAATCATCATTGATCAATATCTTAAATCTTCTTTAGTCGCTGAAAAGAATTCTT
ACCTTTTGGTACTTGCTTTGGCTCCAAAAGAGGAATTGGTTGAAAAAGCCCTCAATTTCG
CTTTATCTCCATCCGTTAGAAGTCAAGATAGTTATATGGTTTTCTTTACCCTCCCAAATC
GTGTTCGTCAATTCGCTTGGGCTTACCTCACCAAAAACTTTAATCAAATCAACGAAACAT
TTAAAAACTCTCCTTTGTTTGGTCGTATCATTGGTAGTTGTCTCACCTCTAAAATGGATG
ATTCTCAATACAATGAAGTCGTCAACTTTTTCAAAGATCATCCAGTTCCAATTGCTGATC
GTTCAATTAAACAAGATTTAGAAATGGTTACAATTAATTCAAATTGGTTCAAAGCTTTTA
ATCAAGATTTATCAAATTGGATTCAATCAAAATAAAAATAAAATCTCTTACAATGAAAGA
TCTTTGTTTTAATAATNATAGGATAAAAAAAAANAGACGAAAACTAAAATAAACATCAAA
TTAT