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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB142
Library VH
(Link to library)
Clone ID VHB142
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16439-1
Original site URL
Representative seq. ID VHB142P
(Link to Original site)
Representative DNA sequence 
>VHB142 (VHB142Q) /CSM/VH/VHB1-B/VHB142Q.Seq.d/
GAACCAAAAATGGATGATGCTTTATATAGCAGACAACTTTATGCATTATCTCATGAAACA
ATGAAAAAAATTACATCAACTTCAGTTTTAGTNGAAGGTTTACAAGGNCXXXXXXXXXXC
AAAGAAGGTTAAGATTCAAACCTNTGAAAATGAACCAGCTCCATCTTCAAACACTCAACA
AGCTGGTGGTGATGCTGAAGATGATCAATGTGATNCCATTTTATCACANCTTCCACAACC
AAGTGAAATGGCTGGTTACANAATTAACTCTATTCNATTTGAAAAGGATGATGATACCAA
TCATCATATCGATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAG
TCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTAC
CACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAA
AGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGT
TGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGA
TCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAA
ACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCAC
TGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCT
CTCAAAGAAACC
sequence update 2002.10.25
Translated Amino Acid sequence 
EPKMDDALYSRQLYALSHETMKKITSTSVLVEGLQG---

---KKVKIQTXENEPAPSSNTQQAGGDAEDDQCDXILSXLPQPSEMAGYXINSIXFEKDD
DTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVI
QNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLF
EKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKK
Translated Amino Acid sequence (All Frames) 
Frame A:
EPKMDDALYSRQLYALSHETMKKITSTSVLVEGLQG---

---qrrlrfkpxkmnqlhlqtlnklvvmlkminvxpfyhxfhnqvkwlvtxltlfxlkrm
mipiiisislplpqi*elptmqsvqlinirpkvspvksfql*lpllp*llvsfvsn*lkl
fkiklwrnirapl*i*vshslvslnqlppqrtkfvkaglghfgivsmlmvislsrsf*is
lkrnmvwifqcslvklhfymlsslirklkrkd*r*kshnsmkpsqrn

Frame B:
nqkwmmlyiadnfmhylmkq*kklhqlqf*xkvykx---

---keg*dsnl*k*tssifkhstsww*c*r*sm*xhfitxsttk*ngwlxn*lysi*kg*
*yqssyrfhyrylkfksyqlcnqss**t*dqryrr*nhssfsyhycrscwfrlyrid*sy
sk*sfgei*ehlyefrypilwfr*tncrpkeqns*rldldtlgsfrc*w*yhsqgvfrsl
*ketwfgyfnall*sytficslh**en*rgkikdenlttl*nplket

Frame C:
tkng*cfi*qttlciis*nneknyinfsfsxrftr---

---KKVKIQTXENEPAPSSNTQQAGGDAEDDQCDXILSXLPQPSEMAGYXINSIXFEKDD
DTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVI
QNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLF
EKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKK
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB142 (VHB142Q) /CSM/VH/VHB1-B/VHB142Q.Seq.d/                       1427   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1304   0.0
SHC572 (SHC572Q) /CSM/SH/SHC5-C/SHC572Q.Seq.d/                       1304   0.0
CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS124Q.Seq.d/                       1304   0.0
VHI150 (VHI150Q) /CSM/VH/VHI1-C/VHI150Q.Seq.d/                       1298   0.0
VHA328 (VHA328Q) /CSM/VH/VHA3-B/VHA328Q.Seq.d/                       1298   0.0
VHA132 (VHA132Q) /CSM/VH/VHA1-B/VHA132Q.Seq.d/                       1298   0.0
SHD220 (SHD220Q) /CSM/SH/SHD2-A/SHD220Q.Seq.d/                       1298   0.0
CHQ706 (CHQ706Q) /CSM/CH/CHQ7-A/CHQ706Q.Seq.d/                       1298   0.0
AHN324 (AHN324Q) /CSM/AH/AHN3-A/AHN324Q.Seq.d/                       1298   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ930776|AZ930776.1 474.dhz58e03.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomycesunisporus genomic clone 474.dhz58e03.s1, genomic survey sequence.    66   8e-13  3
CB933531|CB933531.1 ri31b11.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodi cDNA 5' similar to TR:Q27481 Q27481 C47E12.5 PROTEIN. [1] ;, mRNA sequence.    64   2e-12  2
ED188983|ED188983.1 AUAC-aav62e08.g1 Ascaris suum whole genome shotgun library (PMAJ_4 GSS) Ascaris suum genomic, genomic survey sequence.    60   1e-04  1
BZ303839|BZ303839.1 KD2618.q1 Kluyveromyces delphensis Random Genomic Library Kluyveromyces delphensis genomic clone KD2618, DNA sequence.    46   0.003  2
AL403306|AL403306.1 T3 end of clone AT0AA007G10 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    42   0.003  3
DR028930|DR028930.1 bda010063_A05_11.b1_024.ab1 Antrodia cinnamomea cDNA Library Antrodia cinnamomea cDNA, mRNA sequence.    50   0.005  2
EE674138| ubiquitin activating enzyme [Drosophila melanogaster], mRNA sequence.    54   0.006  1
EE670055| similar to ubiquitin-activating enzyme E1, Chr X [Mus musculus] [Rattus norvegicus], mRNA sequence.    54   0.006  1
DT079054|DT079054.1 AGENCOURT_55716035 NICHD_XGC_FaBN Xenopus laevis cDNA clone IMAGE:8077350 5', mRNA sequence.    40   0.019  2
EE313715|EE313715.1 AGENCOURT_86343377 NICHD_XGC_bone Xenopus laevis cDNA clone IMAGE:8741187 5', mRNA sequence.    40   0.020  2
dna update 2007. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   438   e-122
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   241   3e-62
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   229   6e-59
FN392321_874(FN392321|pid:none) Pichia pastoris GS115 chromosome...   228   2e-58
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   223   4e-57
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   220   4e-56
CR382122_592(CR382122|pid:none) Kluyveromyces lactis strain NRRL...   220   5e-56
AE016819_666(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   219   6e-56
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   219   8e-56
BX908809_46(BX908809|pid:none) Neurospora crassa DNA linkage gro...   219   1e-55
protein update 2009. 6.27
PSORT 

 psg: 0.75  gvh: 0.59  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.12  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	  8.0 %: cytoskeletal
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane

>> prediction for VHB142 is cyt
5' end seq. ID VHB142F
5' end seq. 
>VHB142F.Seq
GAACCAAAAATGGATGATGCTTTATATAGCAGACAACTTTATGCATTATCTCATGAAACA
ATGAAAAAAATTACATCAACTTCAGTTTTAGTNGAAGGTTTACAAGGNCNNNNNNNNNN
Length of 5' end seq. 119
3' end seq. ID VHB142Z
3' end seq. 
>VHB142Z.Seq
NNNNNNNNNNCAAAGAAGGTTAAGATTCAAACCTNTGAAAATGAACCAGCTCCATCTTCA
AACACTCAACAAGCTGGTGGTGATGCTGAAGATGATCAATGTGATNCCATTTTATCACAN
CTTCCACAACCAAGTGAAATGGCTGGTTACANAATTAACTCTATTCNATTTGAAAAGGAT
GATGATACCAATCATCATATCGATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAAC
TATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCA
GCTTTAGTTACCACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTT
ATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGGTATCCCATTC
TTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGG
ACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTC
TTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATAT
GCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTC
TATGAAACCCTCTCAAAGAAACC
Length of 3' end seq. 683
Connected seq. ID VHB142P
Connected seq. 
>VHB142P.Seq
GAACCAAAAATGGATGATGCTTTATATAGCAGACAACTTTATGCATTATCTCATGAAACA
ATGAAAAAAATTACATCAACTTCAGTTTTAGTNGAAGGTTTACAAGGNC----------C
AAAGAAGGTTAAGATTCAAACCTNTGAAAATGAACCAGCTCCATCTTCAAACACTCAACA
AGCTGGTGGTGATGCTGAAGATGATCAATGTGATNCCATTTTATCACANCTTCCACAACC
AAGTGAAATGGCTGGTTACANAATTAACTCTATTCNATTTGAAAAGGATGATGATACCAA
TCATCATATCGATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAG
TCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTAC
CACTACTGCCGTAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAA
AGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGT
TGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGA
TCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAA
ACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCAC
TGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCT
CTCAAAGAAACC
Length of connected seq. 782
Full length Seq ID -
Full length Seq. -
Length of full length seq. -