>VHB117 (VHB117Q) /CSM/VH/VHB1-A/VHB117Q.Seq.d/
GTTATTATTTGTTTGAATTTGGAAATAGCATAATGCAAATTTTCATTAAGACCCTTACTG
GTAAGACCATCACCCTCGAGGTTGAAGGTTCTGATAACATTGAAAATGTTAAAGCCAAAA
TCCAAGATAAAGAAGGTATCCCACCAGATCAACAAAGATTAATCTTTGCCGGTAAACAAT
TAGAAGACGGTCGTACTCTTTCCGATTACAATATCCAAAAAGAATCAACTCTCCACTTAG
TCTTAAGATTAAGAGGTGGTGGTGGTAAAAAGAAGAAGAAGAAGACCTACGCTACCCCAA
AGGTTCTCAAGAGAAAATTAAGAAAAGTTAAATTAGCCGTCCTCAAATACTACAAATTCG
ACGAAAACGGTAAAATCAAACGTGTCTTAAGAGAATGTCCAGCTGAAACCTGTGGTGCTG
GTGTCTTCATGGCTCAACATGCTAATCGTCAATACTGTGGTAAATGTCACTCAACTTTAG
TTAAAAAATCCAAATAAACAATTTTCTTGAAAATATCATAATGCTTATCTAGTATGTGTT
TTTCATGATTAGAGGACGTACTATGCCATACCCAATTAAAACXXXXXXXXXXGTTATTAT
TTGTTTGAATTTGNAAATAGCATAATGCNAATTTTCNNTAAGACCNTTANTGGTAAGACC
ATCACCCTNNAGGTTGAAGGTTCTGATAACATTGAAAATGTTAAAGCCAAAATCCAAGAT
AAANAAGGTATCCCNCCAGATCAACAAAGATTAATCNTNGCCGGTAAACAATTAGAAGAC
GGTCGTACTCTTTCCGATTACAATATCCANAAAGAATCNACTCTCCACTTAGTNTTAAGA
TTAAGAGGTGGTGGTGGTAAAAANA

llfv*iwk*hnanfh*dpyw*dhhprg*rf**h*kc*sqnpr*rryptrstkinlcr*ti
rrrsysfrlqypkrinsplslkikrwww*keeeedlrypkgsqekikks*isrpqilqir
rkr*nqtclkrmss*nlwcwclhgstc*ssilw*mslnfs*kiqinnfleniimli*YVF
FMIRGRTMPYPIK---

---YYLFEFXNSIMXIFXKTXXGKTITLXVEGSDNIENVKAKIQDKXGIPPDQQRLIXAG
KQLEDGRTLSDYNIXKESTLHLVLRLRGGGGKX

Frame A:
viiclnleia*ckfslrpllvrpspsrlkvlitlkmlkpkskikkvshqinkd*slpvnn
*ktvvlfpitiskknqlst*s*d*evvvvkrrrrrptlpqrfsren*ekln*pssnttns
tktvksnvs*envqlkpvvlvsswlnmlivntvvnvtql*lknpnkqfs*kyhnaylvcv
fhd*rtyyaipn*n---

---viiclnlxia*cxfsxrplxvrpspxrlkvlitlkmlkpkskixkvsxqinkd*sxp
vnn*ktvvlfpitisxknxlst*x*d*evvvvkx

Frame B:
llfv*iwk*hnanfh*dpyw*dhhprg*rf**h*kc*sqnpr*rryptrstkinlcr*ti
rrrsysfrlqypkrinsplslkikrwww*keeeedlrypkgsqekikks*isrpqilqir
rkr*nqtclkrmss*nlwcwclhgstc*ssilw*mslnfs*kiqinnfleniimli*YVF
FMIRGRTMPYPIK---

---llfv*ixk*hnanfx*dxxw*dhhpxg*rf**h*kc*sqnpr*xrypxrstkinxxr
*tirrrsysfrlqypxrixsplsxkikrwww*k

Frame C:
yylfefgnsimqifiktltgktitlevegsdnienvkakiqdkegippdqqrlifagkql
edgrtlsdyniqkestlhlvlrlrggggkkkkkktyatpkvlkrklrkvklavlkyykfd
engkikrvlrecpaetcgagvfmaqhanrqycgkchstlvkksk*tiflkis*clssmcf
s*ledvlchtqlk---

---YYLFEFXNSIMXIFXKTXXGKTITLXVEGSDNIENVKAKIQDKXGIPPDQQRLIXAG
KQLEDGRTLSDYNIXKESTLHLVLRLRGGGGKX

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB117 (VHB117Q) /CSM/VH/VHB1-A/VHB117Q.Seq.d/                       1562   0.0
VHJ458 (VHJ458Q) /CSM/VH/VHJ4-C/VHJ458Q.Seq.d/                       1154   0.0
VFK618 (VFK618Q) /CSM/VF/VFK6-A/VFK618Q.Seq.d/                       1154   0.0
VHD646 (VHD646Q) /CSM/VH/VHD6-B/VHD646Q.Seq.d/                       1134   0.0
VHF225 (VHF225Q) /CSM/VH/VHF2-B/VHF225Q.Seq.d/                       1132   0.0
VHE381 (VHE381Q) /CSM/VH/VHE3-D/VHE381Q.Seq.d/                       1130   0.0
FC-AJ24 (FC-AJ24Q) /CSM/FC/FC-AJ/FC-AJ24Q.Seq.d/                     1122   0.0
VSG649 (VSG649Q) /CSM/VS/VSG6-C/VSG649Q.Seq.d/                       1120   0.0
VSH239 (VSH239Q) /CSM/VS/VSH2-B/VSH239Q.Seq.d/                       1116   0.0
FC-AT06 (FC-AT06Q) /CSM/FC/FC-AT/FC-AT06Q.Seq.d/                     1114   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M23750|M23750.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUBV20.  1086   0.0    1
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.  1037   0.0    2
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   262   e-126  2
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   278   e-125  2
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   262   e-120  3
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   254   e-111  3
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   254   e-108  3
CF181895|CF181895.1 ISO3A12R Iso cDNA Isotricha sp. BBF-2003 cDNA similar to PfpUB polyubiquitin 3D7, mRNA sequence.   131   e-100  5
CF181874|CF181874.1 ISO1H6R Iso cDNA Isotricha sp. BBF-2003 cDNA similar to PfpUB polyubiquitin 3D7, mRNA sequence.   131   e-100  5
CF181989|CF181989.1 ISO4G3F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.   131   e-100  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115584_9(AC115584|pid:none) Dictyostelium discoideum chromosom...   243   5e-63
M23750_1(M23750|pid:none) D.discoideum ubiquitin mRNA, complete ...   241   2e-62
AY752793_1(AY752793|pid:none) Culicoides sonorensis clone CsARU ...   204   4e-51
EU139195_1(EU139195|pid:none) Flustra foliacea ubiquinated putat...   200   6e-50
AY552058_1(AY552058|pid:none) Aedes aegypti ribosomal protein S2...   198   2e-49
EU558350_1(EU558350|pid:none) Phoronis muelleri putative ubiquit...   197   4e-49
AE014134_1952(AE014134|pid:none) Drosophila melanogaster chromos...   196   6e-49
AE016820_243(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...   196   6e-49
EU558303_1(EU558303|pid:none) Novocrania anomala putative ubiqui...   196   6e-49
EF633956_1(EF633956|pid:none) Ornithodoros parkeri clone OP-73 u...   196   1e-48

 psg: 0.72  gvh: 0.42  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: mitochondrial

>> prediction for VHB117 is nuc

>VHB117F.Seq
GTTATTATTTGTTTGAATTTGGAAATAGCATAATGCAAATTTTCATTAAGACCCTTACTG
GTAAGACCATCACCCTCGAGGTTGAAGGTTCTGATAACATTGAAAATGTTAAAGCCAAAA
TCCAAGATAAAGAAGGTATCCCACCAGATCAACAAAGATTAATCTTTGCCGGTAAACAAT
TAGAAGACGGTCGTACTCTTTCCGATTACAATATCCAAAAAGAATCAACTCTCCACTTAG
TCTTAAGATTAAGAGGTGGTGGTGGTAAAAAGAAGAAGAAGAAGACCTACGCTACCCCAA
AGGTTCTCAAGAGAAAATTAAGAAAAGTTAAATTAGCCGTCCTCAAATACTACAAATTCG
ACGAAAACGGTAAAATCAAACGTGTCTTAAGAGAATGTCCAGCTGAAACCTGTGGTGCTG
GTGTCTTCATGGCTCAACATGCTAATCGTCAATACTGTGGTAAATGTCACTCAACTTTAG
TTAAAAAATCCAAATAAACAATTTTCTTGAAAATATCATAATGCTTATCTAGTATGTGTT
TTTCATGATTAGAGGACGTACTATGCCATACCCAATTAAAACNNNNNNNNNN

>VHB117Z.Seq
NNNNNNNNNNGTTATTATTTGTTTGAATTTGNAAATAGCATAATGCNAATTTTCNNTAAG
ACCNTTANTGGTAAGACCATCACCCTNNAGGTTGAAGGTTCTGATAACATTGAAAATGTT
AAAGCCAAAATCCAAGATAAANAAGGTATCCCNCCAGATCAACAAAGATTAATCNTNGCC
GGTAAACAATTAGAAGACGGTCGTACTCTTTCCGATTACAATATCCANAAAGAATCNACT
CTCCACTTAGTNTTAAGATTAAGAGGTGGTGGTGGTAAAAANA

>VHB117P.Seq
GTTATTATTTGTTTGAATTTGGAAATAGCATAATGCAAATTTTCATTAAGACCCTTACTG
GTAAGACCATCACCCTCGAGGTTGAAGGTTCTGATAACATTGAAAATGTTAAAGCCAAAA
TCCAAGATAAAGAAGGTATCCCACCAGATCAACAAAGATTAATCTTTGCCGGTAAACAAT
TAGAAGACGGTCGTACTCTTTCCGATTACAATATCCAAAAAGAATCAACTCTCCACTTAG
TCTTAAGATTAAGAGGTGGTGGTGGTAAAAAGAAGAAGAAGAAGACCTACGCTACCCCAA
AGGTTCTCAAGAGAAAATTAAGAAAAGTTAAATTAGCCGTCCTCAAATACTACAAATTCG
ACGAAAACGGTAAAATCAAACGTGTCTTAAGAGAATGTCCAGCTGAAACCTGTGGTGCTG
GTGTCTTCATGGCTCAACATGCTAATCGTCAATACTGTGGTAAATGTCACTCAACTTTAG
TTAAAAAATCCAAATAAACAATTTTCTTGAAAATATCATAATGCTTATCTAGTATGTGTT
TTTCATGATTAGAGGACGTACTATGCCATACCCAATTAAAAC----------GTTATTAT
TTGTTTGAATTTGNAAATAGCATAATGCNAATTTTCNNTAAGACCNTTANTGGTAAGACC
ATCACCCTNNAGGTTGAAGGTTCTGATAACATTGAAAATGTTAAAGCCAAAATCCAAGAT
AAANAAGGTATCCCNCCAGATCAACAAAGATTAATCNTNGCCGGTAAACAATTAGAAGAC
GGTCGTACTCTTTCCGATTACAATATCCANAAAGAATCNACTCTCCACTTAGTNTTAAGA
TTAAGAGGTGGTGGTGGTAAAAANA