>VHB106 (VHB106Q) /CSM/VH/VHB1-A/VHB106Q.Seq.d/
XXXXXXXXXXTTTAGAAACTGCTGGTGGTGTTATGACAACTCTTATTCCAAGAAATACAA
CAATTCCATGTAAGAAAACTCAAACCTTCTCTACATACTCTGATAATCAACCAGGTGTAT
TAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAAT
TTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTG
ATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAAC
AAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGG
TTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAA
AGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTG
CAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCA
AATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCT
TAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAG
GTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAG
ACGAATT

---LETAGGVMTTLIPRNTTIPCKKTQTFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKF
ELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMV
ADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLK
WLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVD
E

Frame A:
---LETAGGVMTTLIPRNTTIPCKKTQTFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKF
ELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMV
ADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLK
WLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVD
E


Frame B:
---*kllvvl*qllfqeiqqfhvrklkpslhtliinqvy*fkfmkvnvl*pkiiiywvnl
nflvfhqhqevyhklklhlmlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkwl
lmqrnsnnkmnnkkivlnqrinwkimhsllkiqlkmkklqpkfqiqinqllnlklkvysn
g*nqikpqkrmnmkir*kp*kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*t
n


Frame C:
---rncwwcydnsyskkynnsm*ensnllyil**strcinssl*r*tcydqr**fig*i*
tfwyststkrctts*syi*c*ckwylkcis*r*inw*qtkdynyq**rssfkrrn*kngc
*creiqttr*ttkrsc*ike*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqm
vriksnrrkg*i*r*deslrsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrr
i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB106 (VHB106Q) /CSM/VH/VHB1-A/VHB106Q.Seq.d/                       1421   0.0
SHE551 (SHE551Q) /CSM/SH/SHE5-C/SHE551Q.Seq.d/                       1421   0.0
SFG805 (SFG805Q) /CSM/SF/SFG8-A/SFG805Q.Seq.d/                       1421   0.0
CHL528 (CHL528Q) /CSM/CH/CHL5-B/CHL528Q.Seq.d/                       1421   0.0
VHP224 (VHP224Q) /CSM/VH/VHP2-A/VHP224Q.Seq.d/                       1415   0.0
CHQ192 (CHQ192Q) /CSM/CH/CHQ1-D/CHQ192Q.Seq.d/                       1415   0.0
SFC313 (SFC313Q) /CSM/SF/SFC3-A/SFC313Q.Seq.d/                       1413   0.0
SFB318 (SFB318Q) /CSM/SF/SFB3-A/SFB318Q.Seq.d/                       1413   0.0
AFE717 (AFE717Q) /CSM/AF/AFE7-A/AFE717Q.Seq.d/                       1413   0.0
VHH751 (VHH751Q) /CSM/VH/VHH7-C/VHH751Q.Seq.d/                       1409   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1421   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1413   0.0    1
EH009913|EH009913.1 USDA-FP_183064 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt016_A01 5', mRNA sequence.   123   2e-61  5
EH011027|EH011027.1 USDA-FP_182007 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt003_D12 5', mRNA sequence.   123   4e-59  5
EH013087|EH013087.1 USDA-FP_185922 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt054_F03 5', mRNA sequence.   123   4e-59  5
EH016245|EH016245.1 USDA-FP_188766 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt091_A11 5', mRNA sequence.   123   4e-59  5
DW218863|DW218863.1 EST35121 Larval Stage 1 Aedes aegypti cDNA clone AEGR-LS1-091-A08-U.AB1 5', mRNA sequence.   194   1e-56  3
EH015809|EH015809.1 USDA-FP_188373 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt085_E08 5', mRNA sequence.   123   6e-56  5
EC823455|EC823455.1 SME00003319 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.   188   2e-55  5
DQ060429|DQ060429.1 Cryptosporidium baileyi strain sbcb heat shock protein 70-like gene, partial sequence.    96   1e-52  5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   408   e-113
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   408   e-113
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   308   8e-83
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   308   8e-83
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   307   2e-82
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   307   2e-82
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   306   5e-82
EF506932_1(EF506932|pid:none) Fasciola hepatica heat shock prote...   305   1e-81
(P22953) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   305   1e-81
S46302(S46302;S41402;JA0169;S36623) dnaK-type molecular chaperon...   305   1e-81

 psg: 0.75  gvh: 0.29  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for VHB106 is nuc

>VHB106Z.Seq
NNNNNNNNNNTTTAGAAACTGCTGGTGGTGTTATGACAACTCTTATTCCAAGAAATACAA
CAATTCCATGTAAGAAAACTCAAACCTTCTCTACATACTCTGATAATCAACCAGGTGTAT
TAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAAT
TTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTG
ATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAAC
AAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGG
TTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAA
AGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTG
CAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCA
AATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCT
TAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAG
GTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAG
ACGAATT