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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHB101
Library VH
(Link to library)
Clone ID VHB101
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16299-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHB101 (VHB101Q) /CSM/VH/VHB1-A/VHB101Q.Seq.d/
XXXXXXXXXXTTCTATGAAAGAGCTGGTCGTGTATCATGTATTGGTCATCCAACTCGTAT
TGGTTCAGTTACAATTGTCGGTGCAGTCTCTCCCCCAGGTGGAGATTTTGCTGATCCAGT
CACTGCTGCCACTTTAGGTATCGTCCAAGTATTCTGGGGTTTAGATAAAAAATTAGCACA
ACGTAAACATTTCCCATCCATCAATTGGTTAATTTCTTTCTCCAAATATATGCAAGCTCT
TGATACTCATTATGATCAAATGGATCCAGAATTCGTTCCACTCCGTACCAGAGCCAAGGA
AATTCTTCAAATGGAAGAAGATCTCTCTGAAATCGTCCAATTGGTCGGTCAAGATTCTTT
AGGTGAATCTGAAAAGATTACCATTGAAGTCGCTCGTATTATTCGTGATGATTTCCTTCA
ACAAAATGGTTTCTCTCCATACGATAAATGTTGTCCATTCTTCAAGACCGTTTGGATGTT
AAAGAATATGATGACTTTNTACAATTTAGCACAAAAAGCTGTTGAATCAAGTACCGCTGA
TAATAAAGTTACTTGGAATCAAATTAAAAATGAATTAAAAGAAATCATTCACAGAATTAC
TTCAATGAAATTCCAAGATCCAACTGACGGTGAACAAACTTTAACTGCTCATTTCTCAAC
TCTCAATGAAGATATCATTACTGCTTTCAGAAACTTTAGTNATTTAGTTTAA
sequence update 2002. 9.10
Translated Amino Acid sequence 
---YERAGRVSCIGHPTRIGSVTIVGAVSPPGGDFADPVTAATLGIVQVFWGLDKKLAQR
KHFPSINWLISFSKYMQALDTHYDQMDPEFVPLRTRAKEILQMEEDLSEIVQLVGQDSLG
ESEKITIEVARIIRDDFLQQNGFSPYDKCCPFFKTVWMLKNMMTXYNLAQKAVESSTADN
KVTWNQIKNELKEIIHRITSMKFQDPTDGEQTLTAHFSTLNEDIITAFRNFSXLV*
Translated Amino Acid sequence (All Frames) 
Frame A:
---smkelvvyhvlviqlvlvqlqlsvqslpqveilliqsllpl*vsskysgv*ikn*hn
vnishpsig*flspnickllilimikwiqnsfhsvpeprkffkwkkislkssnwsvkil*
vnlkrlplkslvlfvmisfnkmvslhtinvvhssrpfgc*ri**lxti*hkkllnqvpli
ikllgiklkmn*kksftellq*nskiqltvnkl*llisqlsmkislllsetlvi*f


Frame B:
---l*kswscimywssnsywfsyncrcslsprwrfc*sshcchfryrpsilgfr*kistt
*tfpihqlvnfflqiyass*ysl*sngsrirstpyqsqgnssngrrsl*nrpigrsrffr
*i*kdyh*srsyys**fpstkwflsir*mlsilqdrldvkeyddfxqfstksc*ikyr**
*sylesn*k*ikrnhsqnyfneiprsn*r*tnfncsflnsq*ryhycfqkl*xfsl


Frame C:
---YERAGRVSCIGHPTRIGSVTIVGAVSPPGGDFADPVTAATLGIVQVFWGLDKKLAQR
KHFPSINWLISFSKYMQALDTHYDQMDPEFVPLRTRAKEILQMEEDLSEIVQLVGQDSLG
ESEKITIEVARIIRDDFLQQNGFSPYDKCCPFFKTVWMLKNMMTXYNLAQKAVESSTADN
KVTWNQIKNELKEIIHRITSMKFQDPTDGEQTLTAHFSTLNEDIITAFRNFSXLV*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB101 (VHB101Q) /CSM/VH/VHB1-A/VHB101Q.Seq.d/                       1380   0.0
VHP385 (VHP385Q) /CSM/VH/VHP3-D/VHP385Q.Seq.d/                       1370   0.0
VHN460 (VHN460Q) /CSM/VH/VHN4-C/VHN460Q.Seq.d/                       1360   0.0
SLE162 (SLE162Q) /CSM/SL/SLE1-C/SLE162Q.Seq.d/                       1360   0.0
VFC688 (VFC688Q) /CSM/VF/VFC6-D/VFC688Q.Seq.d/                       1352   0.0
VFC742 (VFC742Q) /CSM/VF/VFC7-B/VFC742Q.Seq.d/                       1350   0.0
VHP748 (VHP748Q) /CSM/VH/VHP7-B/VHP748Q.Seq.d/                       1348   0.0
VHN664 (VHN664Q) /CSM/VH/VHN6-C/VHN664Q.Seq.d/                       1348   0.0
VHH516 (VHH516Q) /CSM/VH/VHH5-A/VHH516Q.Seq.d/                       1348   0.0
VHD171 (VHD171Q) /CSM/VH/VHD1-C/VHD171Q.Seq.d/                       1348   0.0
own update 2004. 8. 1
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U49169|U49169.1 Dictyostelium discoideum V-ATPase A subunit (vatA) mRNA, complete cds.  1380   0.0    1
BI772943|BI772943.1 kq08f06.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to TR:Q9XW92 Q9XW92 Y49A3A.2 PROTEIN ;, mRNA sequence.   121   6e-35  3
BI073337|BI073337.1 kt29f11.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to TR:Q9XW92 Q9XW92 Y49A3A.2 PROTEIN ;, mRNA sequence.   113   1e-25  3
BI773050|BI773050.1 kq33c01.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to TR:Q9XW92 Q9XW92 Y49A3A.2 PROTEIN ;, mRNA sequence.   121   3e-23  1
DY886672|DY886672.1 CeleSEQ2416 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ2416, mRNA sequence.    82   6e-23  2
EE002467|EE002467.1 ROE00003399 Rhizopus oryzae Company Rhizopus oryzae cDNA, mRNA sequence.    54   4e-22  4
EE002706|EE002706.1 ROE00009688 Rhizopus oryzae Company Rhizopus oryzae cDNA, mRNA sequence.    54   4e-22  4
EE002674|EE002674.1 ROE00009784 Rhizopus oryzae Company Rhizopus oryzae cDNA, mRNA sequence.    54   4e-22  4
BI772898|BI772898.1 kp97e10.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to TR:Q9XW92 Q9XW92 Y49A3A.2 PROTEIN ;, mRNA sequence.    80   9e-19  3
BI703941|BI703941.1 kt23d10.y3 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to TR:Q9XW92 Q9XW92 Y49A3A.2 PROTEIN ;, mRNA sequence.   105   2e-18  1
dna update 2007. 3.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54647) RecName: Full=V-type proton ATPase catalytic subunit A;...   468   e-131
(P38607) Vacuolar ATP synthase catalytic subunit A, osteoclast i...   325   8e-88
A56807(A56807) H+-transporting two-sector ATPase (EC 3.6.3.14), ...   324   2e-87
I50715(I50715) A2 isoform of vacuolar H+-ATPase subunit A - chic...   322   9e-87
(Q90647) RecName: Full=V-type proton ATPase catalytic subunit A;...   322   9e-87
AX407287_1(AX407287|pid:none) Sequence 58 from Patent WO0226984.      321   1e-86
(P31404) RecName: Full=V-type proton ATPase catalytic subunit A;...   321   2e-86
M80430_1(M80430|pid:none) Bovine vacuolar H+-ATPase A subunit mR...   321   2e-86
BC105145_1(BC105145|pid:none) Bos taurus ATPase, H+ transporting...   321   2e-86
(P31400) RecName: Full=V-type proton ATPase catalytic subunit A;...   320   3e-86
protein update 2009. 6.27
PSORT 

 psg: 0.83  gvh: 0.39  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHB101 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHB101Z
3' end seq. 
>VHB101Z.Seq
NNNNNNNNNNTTCTATGAAAGAGCTGGTCGTGTATCATGTATTGGTCATCCAACTCGTAT
TGGTTCAGTTACAATTGTCGGTGCAGTCTCTCCCCCAGGTGGAGATTTTGCTGATCCAGT
CACTGCTGCCACTTTAGGTATCGTCCAAGTATTCTGGGGTTTAGATAAAAAATTAGCACA
ACGTAAACATTTCCCATCCATCAATTGGTTAATTTCTTTCTCCAAATATATGCAAGCTCT
TGATACTCATTATGATCAAATGGATCCAGAATTCGTTCCACTCCGTACCAGAGCCAAGGA
AATTCTTCAAATGGAAGAAGATCTCTCTGAAATCGTCCAATTGGTCGGTCAAGATTCTTT
AGGTGAATCTGAAAAGATTACCATTGAAGTCGCTCGTATTATTCGTGATGATTTCCTTCA
ACAAAATGGTTTCTCTCCATACGATAAATGTTGTCCATTCTTCAAGACCGTTTGGATGTT
AAAGAATATGATGACTTTNTACAATTTAGCACAAAAAGCTGTTGAATCAAGTACCGCTGA
TAATAAAGTTACTTGGAATCAAATTAAAAATGAATTAAAAGAAATCATTCACAGAATTAC
TTCAATGAAATTCCAAGATCCAACTGACGGTGAACAAACTTTAACTGCTCATTTCTCAAC
TCTCAATGAAGATATCATTACTGCTTTCAGAAACTTTAGTNATTTAGTTTAA
Length of 3' end seq. 712
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -