>VHA842 (VHA842Q) /CSM/VH/VHA8-B/VHA842Q.Seq.d/
XXXXXXXXXXTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACATT
CAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCA
TCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGT
CGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATT
TTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCA
CATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAA
TATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATT
CAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATT
TATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACT
ATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAA
TTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAAC

---QPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGR
GVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQY
QGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTI
FSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKN

Frame A:
---stnqw*lctcecycxhsrs*siitihhch**kswmcivkrwsigyddassyfer*ws
wcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefsi
srclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfny
lfnnynhfcn*nefnwcskiikfikikmenc*w*k


Frame B:
---QPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGR
GVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQY
QGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTI
FSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKN


Frame C:
---nqpvvimyl*mllxtfkilinhynsplslievvdvhr*emvnwi**civvl*kmmvv
vlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigilni
kvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifqls
fqqlqsllqlk*i*lvfknyqiyqd*ngkllmvk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1259   0.0
VHP841 (VHP841Q) /CSM/VH/VHP8-B/VHP841Q.Seq.d/                       1259   0.0
VHP823 (VHP823Q) /CSM/VH/VHP8-A/VHP823Q.Seq.d/                       1259   0.0
VHP767 (VHP767Q) /CSM/VH/VHP7-C/VHP767Q.Seq.d/                       1259   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1259   0.0
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                       1259   0.0
VHO880 (VHO880Q) /CSM/VH/VHO8-D/VHO880Q.Seq.d/                       1259   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1259   0.0
VHN718 (VHN718Q) /CSM/VH/VHN7-A/VHN718Q.Seq.d/                       1259   0.0
VHN593 (VHN593Q) /CSM/VH/VHN5-D/VHN593Q.Seq.d/                       1259   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1235   0.0    1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   0.072  8
AP007341|AP007341.1 Lotus japonicus genomic DNA, chromosome 2, clone:LjT35G04, TM0425.    40   0.10   2
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   1.2    1
AM025232|AM025232.1 Bos indicus 3' EST, clone C0005915l21.         34   3.1    2
BH703301|BH703301.1 BOMNG55TR BO_2_3_KB Brassica oleracea genomic clone BOMNG55, DNA sequence.    40   3.2    2
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   3.8    2
EH243429|EH243429.1 JGI_ACAC13551.fwd ACAC Phakopsora pachyrhizi TW72-1 germinating urediniospores on water surface (20C/16 hours/dark) Phakopsora pachyrhizi cDNA clone ACAC13551 5', mRNA sequence.    44   4.7    1
AV262947|AV262947.1 Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930432P20, 3' end partial sequence.    44   4.7    1
EH220383|EH220383.1 JGI_ABWZ1277.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA clone ABWZ1277 3', mRNA sequence.    44   4.7    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   358   1e-97
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   358   1e-97
BT036325_1(BT036325|pid:none) Zea mays full-length cDNA clone ZM...    92   2e-17
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...    91   4e-17
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...    89   1e-16
EF146940_1(EF146940|pid:none) Populus trichocarpa clone WS01225_...    87   4e-16
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...    86   7e-16
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...    86   1e-15
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...    86   1e-15
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...    84   4e-15

 psg: 0.82  gvh: 0.45  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHA842 is cyt

>VHA842Z.Seq
NNNNNNNNNNTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCNTACATT
CAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCA
TCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGT
CGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATT
TTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCA
CATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAA
TATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATT
CAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATT
TATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACT
ATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAA
TTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAAC