>VHA801 (VHA801Q) /CSM/VH/VHA8-A/VHA801Q.Seq.d/
AAGTGGTATGAGTCATGATGAAGATCAATTAATTCCAAATCTTTATCGTTACATTCAACC
ATGGGAACAAGAAATTAAAGATAGTCAACGTGTTTGGGCAGAATATGCAATCAAATATGA
AGAGGCTAAATCACAAAATAAGAATCTTACATTGGAGGATCTTGAAGATAGTTGGGATCG
TGGATACCACGTATCAATACATTATTCCAAAAGAGTCGTCATACTTTAGCTTATGATAAA
GGTTGGAGAGTAAGAACCGATTGGAAACAATATCAAGTCCTAAAGAATAATCCATTCTGG
TGGACTAATCAACGTCATGATGGTAAACTTTGGAATCTTAATAATTATCGTACAGATATC
ATTCAAGCATTGGGTGGTGTTGAAGGTATTCTAGAGCATACTCTATTCAAAGGTACTTAT
TTCCCAACTTGGGAAGGTTTATTTTGGGAGAAAGCATCAGGTTTCGAAGAGTCTATGAAA
TATAAAAAACTTACACACGCTCAACGTTCAGGTCTTAATCAAATTCCAAATCGTCGTTTC
ACACTTTGGTGGTCTCCAACTATCAATCGTAAGAATGTTTATGTTXXXXXXXXXXGCTAA
AGAAATTGGTGGTTTCACTTATGTTTTCCCAAAGAATATCTTAAAGAAATTCATTACCAT
TGCCGATCTTCGTACTCAAATCATGGGTTATTGTTATGGTATCTCACCACCCGATAATCC
ATCAGTAAAAGAAATTCGTTGTATTGTAATGCCACCACAATGGGGTACACCAGTTCATGT
AACCGTACCAAATCAATTACCAGAACATGAATATCTCAAAGATTTAGAACCATTGGGTTG
GATTCACACTCAACCAACTGAATTACCACAACTATCACCACAAGATGTTATCACTCATTC
AAAGATTATGTCTGACAATAAGAGTTGGGATGGTGAGAAAACTGTTATCATCTCTGTCTC
TGTGGCTTGGCCTTGTACTCTAACTGCTTACCATTTGACTCCAAGTGGTTTTGAATGGGG
TAAAAATAATAAAGATTCTCTCAATTATCAAGGTTATCAACCACAATTCTATGAGAAGGT
TCAAATGTTATTATCCGATCGTTTCCTTGGTTTCTATATGGTACCTGATCGTGGTTCTTG
GAATTATAATTTCATGGGTGTTAAACATTCCACAAATATGACCTATGGTTTGAAATTAGA
TTATCCTAAAAACTTTTATGATGAATCTCATAGACCTGCTCATTTCCAAAATTGGACTCA
AATGGCTCCTTCAGCAAATGATGATGAAGAAAATCAACCNAAAANAAAATTTATTTNAAT
AATTATAAAAATAATAATTTTCTTTTAAAAACATTTAATAAATAAATAAAAAAA

kwyes**rsinskslslhstmgtrn*r*stclgricnqi*rg*itk*esyiggs*r*LGS
WIPRINTLFQKSRHTLAYDKGWRVRTDWKQYQVLKNNPFWWTNQRHDGKLWNLNNYRTDI
IQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTHAQRSGLNQIPNRRF
TLWWSPTINRKNVYV---

---AKEIGGFTYVFPKNILKKFITIADLRTQIMGYCYGISPPDNPSVKEIRCIVMPPQWG
TPVHVTVPNQLPEHEYLKDLEPLGWIHTQPTELPQLSPQDVITHSKIMSDNKSWDGEKTV
IISVSVAWPCTLTAYHLTPSGFEWGKNNKDSLNYQGYQPQFYEKVQMLLSDRFLGFYMVP
DRGSWNYNFMGVKHSTNMTYGLKLDYPKNFYDESHRPAHFQNWTQMAPSANDDEENQPKX
KFIXIIIKIIIFF*khlink*k

Frame A:
kwyes**rsinskslslhstmgtrn*r*stclgricnqi*rg*itk*esyiggs*r*LGS
WIPRINTLFQKSRHTLAYDKGWRVRTDWKQYQVLKNNPFWWTNQRHDGKLWNLNNYRTDI
IQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTHAQRSGLNQIPNRRF
TLWWSPTINRKNVYV---

---AKEIGGFTYVFPKNILKKFITIADLRTQIMGYCYGISPPDNPSVKEIRCIVMPPQWG
TPVHVTVPNQLPEHEYLKDLEPLGWIHTQPTELPQLSPQDVITHSKIMSDNKSWDGEKTV
IISVSVAWPCTLTAYHLTPSGFEWGKNNKDSLNYQGYQPQFYEKVQMLLSDRFLGFYMVP
DRGSWNYNFMGVKHSTNMTYGLKLDYPKNFYDESHRPAHFQNWTQMAPSANDDEENQPKX
KFIXIIIKIIIFF*khlink*k

Frame B:
sgmshdedqlipnlyryiqpweqeikdsqrvwaeyaikyeeaksqnknltledledswdr
gyhvsihyskrvvil*lmikvge*epignniks*riihsgglinvmmvnfgiliiivqis
fkhwvvlkvf*silyskvlisqlgkvyfgrkhqvsksl*niknlhtlnvqvlikfqivvs
hfgglqlsivrmfm---

---lkklvvslmfsqris*rnslplpifvlkswvivmvshhpiihq*kkfvvl*chhngv
hqfm*pyqinyqnmniski*nhwvgftlnqlnyhnyhhkmlsliqrlcltirvgmvrkll
sslslwlglvl*llti*lqvvlngvkiikilsiikvinhnsmrrfkcyypivslvsiwyl
ivvlgiiiswvlnipqi*pmv*n*iilktfmmnlidlliskiglkwllqqmmmkkinxkx
nlfx*l*k**fsfkni**inkk

Frame C:
vv*vmmkin*fqifivtfnhgnkklkivnvfgqnmqsnmkrlnhkirilhwrilkivgiv
dttyqyiipkessyfsl**rlesknrletisspke*silvd*sts*w*tles**lsyryh
ssigwc*rysraysiqrylfpnlgrfilgesirfrrvyei*ktytrstfrs*snskssfh
tlvvsnyqs*eclc---

---*rnwwfhlcfpkeylkeihyhcrssysnhglllwylttr*siskrnslycnattmgy
tsscnrtksitrt*isqrfrtigldshstn*itttittrcyhsfkdyv*q*elgw*ency
hlclcglalysnclpfdskwf*mg*k**rfsqlsrlsttil*egsnviirsfpwflygt*
swflel*fhgc*tfhkydlwfeirls*kll**is*tcsfpkldsngsfsk***rkstxxk
iyxnnyknnnfllktfnk*ikk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA801 (VHA801Q) /CSM/VH/VHA8-A/VHA801Q.Seq.d/                       2516   0.0
AHF452 (AHF452Q) /CSM/AH/AHF4-C/AHF452Q.Seq.d/                       1348   0.0
AHN642 (AHN642Q) /CSM/AH/AHN6-B/AHN642Q.Seq.d/                       1195   0.0
FC-AI07 (FC-AI07Q) /CSM/FC/FC-AI/FC-AI07Q.Seq.d/                     1191   0.0
AHI237 (AHI237Q) /CSM/AH/AHI2-B/AHI237Q.Seq.d/                       1068   0.0
FC-BT07 (FC-BT07Q) /CSM/FC/FC-BT/FC-BT07Q.Seq.d/                      940   0.0
CFD164 (CFD164Q) /CSM/CF/CFD1-C/CFD164Q.Seq.d/                        737   0.0
SSL142 (SSL142Q) /CSM/SS/SSL1-B/SSL142Q.Seq.d/                         38   0.35
SLE396 (SLE396Q) /CSM/SL/SLE3-D/SLE396Q.Seq.d/                         38   0.35
VSF593 (VSF593Q) /CSM/VS/VSF5-D/VSF593Q.Seq.d/                         36   1.4

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1364   0.0    1
DY893369|DY893369.1 CeleSEQ12690 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ12690, mRNA sequence.   218   3e-83  3
CZ529473|CZ529473.1 SRAA-aac61d08.g1 Strongyloides ratti whole genome shotgun library (SRAAGSS 004) Strongyloides ratti genomic, genomic survey sequence.   111   6e-57  4
DT614605| CG8877-PA [Drosophila melanogaster], mRNA sequence.   159   1e-48  3
AF397148|AF397148.1 Paramecium tetraurelia macronuclear pre-mRNA processing factor 8 gene, complete cds.   119   3e-43  10
CA217231|CA217231.1 SCSFAD1125C07.g AD1 Saccharum officinarum cDNA clone SCSFAD1125C07 5', mRNA sequence.   180   6e-42  2
DY887341|DY887341.1 CeleSEQ3300 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ3300, mRNA sequence.   117   8e-42  2
CA098022|CA098022.1 SCMCCL6053A12.g CL6 Saccharum officinarum cDNA clone SCMCCL6053A12 5', mRNA sequence.   180   7e-41  1
AC190756|AC190756.3 Zea mays chromosome 9 clone CH201-223D9; ZMMBBc0223D09, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.   178   8e-41  7
AC190756|AC190756.3 Zea mays chromosome 9 clone CH201-223D9; ZMMBBc0223D09, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.   178   9e-41  7

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8T295) RecName: Full=Pre-mRNA-processing-splicing factor 8 hom...   508   e-142
AB023482_13(AB023482|pid:none) Oryza sativa Japonica Group genom...   308   e-101
AK099780_1(AK099780|pid:none) Oryza sativa Japonica Group cDNA c...   308   e-101
AE013599_1462(AE013599|pid:none) Drosophila melanogaster chromos...   317   e-100
AL591496_12(AL591496|pid:none) Mouse DNA sequence from clone RP2...   322   e-100
(Q99PV0) RecName: Full=Pre-mRNA-processing-splicing factor 8; Al...   322   e-100
BC064370_1(BC064370|pid:none) Homo sapiens PRP8 pre-mRNA process...   322   e-100
BC045266_1(BC045266|pid:none) Xenopus laevis pre-mRNA processing...   320   e-100
BC034648_1(BC034648|pid:none) Mus musculus pre-mRNA processing f...   322   e-100
AK296344_1(AK296344|pid:none) Homo sapiens cDNA FLJ57882 complet...   318   e-100

 psg: 0.69  gvh: 0.37  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 24.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHA801 is mit

>VHA801F.Seq
AAGTGGTATGAGTCATGATGAAGATCAATTAATTCCAAATCTTTATCGTTACATTCAACC
ATGGGAACAAGAAATTAAAGATAGTCAACGTGTTTGGGCAGAATATGCAATCAAATATGA
AGAGGCTAAATCACAAAATAAGAATCTTACATTGGAGGATCTTGAAGATAGTTGGGATCG
TGGATACCACGTATCAATACATTATTCCAAAAGAGTCGTCATACTTTAGCTTATGATAAA
GGTTGGAGAGTAAGAACCGATTGGAAACAATATCAAGTCCTAAAGAATAATCCATTCTGG
TGGACTAATCAACGTCATGATGGTAAACTTTGGAATCTTAATAATTATCGTACAGATATC
ATTCAAGCATTGGGTGGTGTTGAAGGTATTCTAGAGCATACTCTATTCAAAGGTACTTAT
TTCCCAACTTGGGAAGGTTTATTTTGGGAGAAAGCATCAGGTTTCGAAGAGTCTATGAAA
TATAAAAAACTTACACACGCTCAACGTTCAGGTCTTAATCAAATTCCAAATCGTCGTTTC
ACACTTTGGTGGTCTCCAACTATCAATCGTAAGAATGTTTATGTTNNNNNNNNNN

>VHA801Z.Seq
NNNNNNNNNNGCTAAAGAAATTGGTGGTTTCACTTATGTTTTCCCAAAGAATATCTTAAA
GAAATTCATTACCATTGCCGATCTTCGTACTCAAATCATGGGTTATTGTTATGGTATCTC
ACCACCCGATAATCCATCAGTAAAAGAAATTCGTTGTATTGTAATGCCACCACAATGGGG
TACACCAGTTCATGTAACCGTACCAAATCAATTACCAGAACATGAATATCTCAAAGATTT
AGAACCATTGGGTTGGATTCACACTCAACCAACTGAATTACCACAACTATCACCACAAGA
TGTTATCACTCATTCAAAGATTATGTCTGACAATAAGAGTTGGGATGGTGAGAAAACTGT
TATCATCTCTGTCTCTGTGGCTTGGCCTTGTACTCTAACTGCTTACCATTTGACTCCAAG
TGGTTTTGAATGGGGTAAAAATAATAAAGATTCTCTCAATTATCAAGGTTATCAACCACA
ATTCTATGAGAAGGTTCAAATGTTATTATCCGATCGTTTCCTTGGTTTCTATATGGTACC
TGATCGTGGTTCTTGGAATTATAATTTCATGGGTGTTAAACATTCCACAAATATGACCTA
TGGTTTGAAATTAGATTATCCTAAAAACTTTTATGATGAATCTCATAGACCTGCTCATTT
CCAAAATTGGACTCAAATGGCTCCTTCAGCAAATGATGATGAAGAAAATCAACCNAAAAN
AAAATTTATTTNAATAATTATAAAAATAATAATTTTCTTTTAAAAACATTTAATAAATAA
ATAAAAAAA

>VHA801P.Seq
AAGTGGTATGAGTCATGATGAAGATCAATTAATTCCAAATCTTTATCGTTACATTCAACC
ATGGGAACAAGAAATTAAAGATAGTCAACGTGTTTGGGCAGAATATGCAATCAAATATGA
AGAGGCTAAATCACAAAATAAGAATCTTACATTGGAGGATCTTGAAGATAGTTGGGATCG
TGGATACCACGTATCAATACATTATTCCAAAAGAGTCGTCATACTTTAGCTTATGATAAA
GGTTGGAGAGTAAGAACCGATTGGAAACAATATCAAGTCCTAAAGAATAATCCATTCTGG
TGGACTAATCAACGTCATGATGGTAAACTTTGGAATCTTAATAATTATCGTACAGATATC
ATTCAAGCATTGGGTGGTGTTGAAGGTATTCTAGAGCATACTCTATTCAAAGGTACTTAT
TTCCCAACTTGGGAAGGTTTATTTTGGGAGAAAGCATCAGGTTTCGAAGAGTCTATGAAA
TATAAAAAACTTACACACGCTCAACGTTCAGGTCTTAATCAAATTCCAAATCGTCGTTTC
ACACTTTGGTGGTCTCCAACTATCAATCGTAAGAATGTTTATGTT----------GCTAA
AGAAATTGGTGGTTTCACTTATGTTTTCCCAAAGAATATCTTAAAGAAATTCATTACCAT
TGCCGATCTTCGTACTCAAATCATGGGTTATTGTTATGGTATCTCACCACCCGATAATCC
ATCAGTAAAAGAAATTCGTTGTATTGTAATGCCACCACAATGGGGTACACCAGTTCATGT
AACCGTACCAAATCAATTACCAGAACATGAATATCTCAAAGATTTAGAACCATTGGGTTG
GATTCACACTCAACCAACTGAATTACCACAACTATCACCACAAGATGTTATCACTCATTC
AAAGATTATGTCTGACAATAAGAGTTGGGATGGTGAGAAAACTGTTATCATCTCTGTCTC
TGTGGCTTGGCCTTGTACTCTAACTGCTTACCATTTGACTCCAAGTGGTTTTGAATGGGG
TAAAAATAATAAAGATTCTCTCAATTATCAAGGTTATCAACCACAATTCTATGAGAAGGT
TCAAATGTTATTATCCGATCGTTTCCTTGGTTTCTATATGGTACCTGATCGTGGTTCTTG
GAATTATAATTTCATGGGTGTTAAACATTCCACAAATATGACCTATGGTTTGAAATTAGA
TTATCCTAAAAACTTTTATGATGAATCTCATAGACCTGCTCATTTCCAAAATTGGACTCA
AATGGCTCCTTCAGCAAATGATGATGAAGAAAATCAACCNAAAANAAAATTTATTTNAAT
AATTATAAAAATAATAATTTTCTTTTAAAAACATTTAATAAATAAATAAAAAAA