>VHA717 (VHA717Q) /CSM/VH/VHA7-A/VHA717Q.Seq.d/
ATTGAAAAAGACATAAATATATAATAAATAATGTCAGAACCAAAACCAACTCAACCAATC
AAAGAGATTGATGAGGAAACTAAAAGAAAGAATGAAGAGAAAAAGAAAGCCAAAGAGGCA
GAGAAAGCAGAGAAATTAGCAAAAATGAAAGAAAAGGAAGCTAAACAAGCAGCAGCAAAA
GAAAAAGAATTACAAAATAAACTTAAAAAAGAACAAGAGAAAAAAGAAAAAGAAGCTGAA
GAAAAAGAAAAACAAGAAGCTGAAGAAAAAATTGTTAGAGAACATATTGAAAAAATTTTA
AAAACCCCAAAGGGTGAAAAGGAAGATGTTTCAAGTTTATTATCATCATATCATCCAACA
GCAGTTGAATCAATTTGGTATGATTATTGGTTAGATAATGGATATTTTTCACCAGAGAAA
CAAATGGAGATTCAACCACATGTAGTTAAAGATAAAAAGTTTGTAATTGTTATTCCACCA
CCAAATGTTACAGGTAGTTTACATTTAGGACATGCATTAACCAATTCAATTCAAGATGCA
GTTGTTCGTTATCATCGTATGAAGGGTGAAGTTTGTTTATGGGTACCAGGTACAGATCAT
GCAGGTATTGCAACACAAGTAGTTGTAGAGAAAAAGATTTGGAAAGAGXXXXXXXXXXAA
AGAAACCCTTTACACTTGTATCGATATTGGTCTTCGTTTATTACATCCATTCATGCCATA
CCTCACCGAGGAGTTGTATCAATCATTACCACGTCGTCCAGAAGATGTCATTCCAACCAT
TATGTTATGTCCATACCCACAATCTCAATCATGTTGGGCCAATTCTGACATTGAGGAAGA
AATGAAACAATGTCAAGATATCATCAAATCAATTCGTTCACTTCGTGCTACCTATACCAT
TCCAGCCTCTAAAAAACTTCACACTTATCTTCACGTTAAAAATCAAGAGAATCTCGAACG
TTTCACTAAAAACTCTTCATTCATTCGTGTTTTAGCTTATGCCTCTGAACTCGAAGTTTC
CATTAGTGATGAATCTCGTCCTGGTTGTATCGTTAATGTTGTCAATGAAAATGTTTCAAC
CCTTTTAGATGTTAGAGGATCTGTTGATTTCAATCTTGAAATTGCTCGTCTCGAAACAAA
GAAACAACAATTGGTCAAAAACTATGAAACTTTAGTTTCAAAAACTACCATACCTTCTTA
TGATAAAGTTCCACAAAATATCAGAGATGATAATACTCAAAAAATTAAAGCTTTAGATGA
AGAAATTAATATTACAAACAAAACCATTGAAAGTTTTACAAAATTAAATATTTAAAATTA
TAAATAATAATTAATAATAATAATAAAAAGTTTNATACTAAATT

iekdini**IMSEPKPTQPIKEIDEETKRKNEEKKKAKEAEKAEKLAKMKEKEAKQAAAK
EKELQNKLKKEQEKKEKEAEEKEKQEAEEKIVREHIEKILKTPKGEKEDVSSLLSSYHPT
AVESIWYDYWLDNGYFSPEKQMEIQPHVVKDKKFVIVIPPPNVTGSLHLGHALTNSIQDA
VVRYHRMKGEVCLWVPGTDHAGIATQVVVEKKIWKE---

---KETLYTCIDIGLRLLHPFMPYLTEELYQSLPRRPEDVIPTIMLCPYPQSQSCWANSD
IEEEMKQCQDIIKSIRSLRATYTIPASKKLHTYLHVKNQENLERFTKNSSFIRVLAYASE
LEVSISDESRPGCIVNVVNENVSTLLDVRGSVDFNLEIARLETKKQQLVKNYETLVSKTT
IPSYDKVPQNIRDDNTQKIKALDEEINITNKTIESFTKLNI*nyk**liiiiksxiln

Frame A:
iekdini**IMSEPKPTQPIKEIDEETKRKNEEKKKAKEAEKAEKLAKMKEKEAKQAAAK
EKELQNKLKKEQEKKEKEAEEKEKQEAEEKIVREHIEKILKTPKGEKEDVSSLLSSYHPT
AVESIWYDYWLDNGYFSPEKQMEIQPHVVKDKKFVIVIPPPNVTGSLHLGHALTNSIQDA
VVRYHRMKGEVCLWVPGTDHAGIATQVVVEKKIWKE---

---KETLYTCIDIGLRLLHPFMPYLTEELYQSLPRRPEDVIPTIMLCPYPQSQSCWANSD
IEEEMKQCQDIIKSIRSLRATYTIPASKKLHTYLHVKNQENLERFTKNSSFIRVLAYASE
LEVSISDESRPGCIVNVVNENVSTLLDVRGSVDFNLEIARLETKKQQLVKNYETLVSKTT
IPSYDKVPQNIRDDNTQKIKALDEEINITNKTIESFTKLNI*nyk**liiiiksxiln

Frame B:
lkkt*iynk*cqnqnqlnqskrlmrklkermkrkrkpkrqrkqrn*qk*kkrklnkqqqk
kknykinlkknkrkkkkklkkkknkklkkkllenilkkf*kpqrvkrkmfqvyyhhiiqq
qlnqfgmiig*imdifhqrnkwrfnhm*lkiksl*llfhhqmlqvvyi*dmh*piqfkmq
lfviiv*rvkfvygyqvqimqvlqhk*l*rkrfgk---

---kkpftlvsilvfvyyihschtsprscinhyhvvqkmsfqplcyvhthnlnhvgpilt
lrkk*nnvkissnqfvhfvlpipfqplknftliftlkikrisnvslktlhsfvf*lmpln
skfplvmnlvlvvslmlsmkmfqpf*mledllisilkllvskqrnnnwsktmkl*fqklp
yllmikfhkisemiilkklkl*mkklilqtkplkvlqn*ifkiinnn*****kvxy*i

Frame C:
*krhkyiinnvrtktnstnqrd**gn*kke*rekesqrgresreisknerkgs*tssskr
kritk*t*krtrekrkrs*rkrktrs*rknc*rty*knfknpkg*kgrcfkfiiiissns
s*inlv*llvr*wifftretngdsttcs*r*kvcncysttkcyr*ftfrtcinqfnsrcs
cslssyeg*slfmgtryrscrycntsscrekdler---

---rnplhlyrywssfitsihaiphrgvvsiittssrrchsnhyvmsiptisimlgqf*h
*grnetmsryhqinsftscylyhssl*ktshlssr*ksresrtfh*klfihscfslcl*t
rsfh***isswlyr*ccq*kcfnpfrc*ric*fqs*ncssrnkettigqkl*nfsfknyh
tfl**sstkyqr**yskn*sfr*rn*yykqnh*kfykikylkl*iiinnnnkkfxtk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA717 (VHA717Q) /CSM/VH/VHA7-A/VHA717Q.Seq.d/                       1976   0.0
SHD476 (SHD476Q) /CSM/SH/SHD4-D/SHD476Q.Seq.d/                       1269   0.0
VHP692 (VHP692Q) /CSM/VH/VHP6-D/VHP692Q.Seq.d/                       1263   0.0
VHL460 (VHL460Q) /CSM/VH/VHL4-C/VHL460Q.Seq.d/                       1253   0.0
SHG276 (SHG276Q) /CSM/SH/SHG2-D/SHG276Q.Seq.d/                       1253   0.0
VHM840 (VHM840Q) /CSM/VH/VHM8-B/VHM840Q.Seq.d/                       1247   0.0
VHL308 (VHL308Q) /CSM/VH/VHL3-A/VHL308Q.Seq.d/                       1247   0.0
SHA541 (SHA541Q) /CSM/SH/SHA5-B/SHA541Q.Seq.d/                       1233   0.0
SHF791 (SHF791Q) /CSM/SH/SHF7-D/SHF791Q.Seq.d/                       1227   0.0
AHC525 (AHC525Q) /CSM/AH/AHC5-B/AHC525Q.Seq.d/                       1187   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.  1197   0.0    1
AB092433|AB092433.1 Entamoeba histolytica mRNA for valyl tRNA synthetase, partial cds.    96   6e-28  2
BH161129|BH161129.1 ENTSE35TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    84   2e-24  2
BH159895|BH159895.1 ENTRU27TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    72   7e-21  2
AZ550882|AZ550882.1 ENTEC77TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    84   2e-20  3
ER385311|ER385311.1 1094420000356 Global-Ocean-Sampling_GS-34-01-01-1P5-1P8KB marine metagenome genomic clone 1061003703946 5', genomic survey sequence.    70   1e-17  2
EJ269346|EJ269346.1 1095353000516 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061005465310 3', genomic survey sequence.    66   9e-17  2
EK366368|EK366368.1 1095469399586 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005857529 3', genomic survey sequence.    70   4e-16  2
EJ196070|EJ196070.1 1092344238471 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001515622 3', genomic survey sequence.    62   3e-13  2
EJ689534|EJ689534.1 1092955394462 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061001892880 5', genomic survey sequence.    68   3e-13  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86KU2) RecName: Full=Probable valyl-tRNA synthetase, cytoplasm...   435   e-120
CR954203_287(CR954203|pid:none) Ostreococcus tauri strain OTTH05...   144   1e-32
BC090426_1(BC090426|pid:none) Danio rerio valyl-tRNA synthetase,...   140   8e-32
BC084762_1(BC084762|pid:none) Xenopus laevis hypothetical LOC495...   135   3e-30
AF506220_1(AF506220|pid:none) Danio rerio valyl-tRNA synthetase ...   133   1e-29
AB092433_1(AB092433|pid:none) Entamoeba histolytica mRNA for val...   133   2e-29
AE013599_1725(AE013599|pid:none) Drosophila melanogaster chromos...   130   8e-29
AB008527_1(AB008527|pid:none) Trichomonas vaginalis gene for Val...   129   2e-28
AB008526_1(AB008526|pid:none) Trichomonas vaginalis gene for Val...   129   2e-28
FN357900_10(FN357900|pid:none) Schistosoma mansoni genome sequen...   129   3e-28

 psg: 0.61  gvh: 0.07  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VHA717 is nuc

>VHA717F.Seq
ATTGAAAAAGACATAAATATATAATAAATAATGTCAGAACCAAAACCAACTCAACCAATC
AAAGAGATTGATGAGGAAACTAAAAGAAAGAATGAAGAGAAAAAGAAAGCCAAAGAGGCA
GAGAAAGCAGAGAAATTAGCAAAAATGAAAGAAAAGGAAGCTAAACAAGCAGCAGCAAAA
GAAAAAGAATTACAAAATAAACTTAAAAAAGAACAAGAGAAAAAAGAAAAAGAAGCTGAA
GAAAAAGAAAAACAAGAAGCTGAAGAAAAAATTGTTAGAGAACATATTGAAAAAATTTTA
AAAACCCCAAAGGGTGAAAAGGAAGATGTTTCAAGTTTATTATCATCATATCATCCAACA
GCAGTTGAATCAATTTGGTATGATTATTGGTTAGATAATGGATATTTTTCACCAGAGAAA
CAAATGGAGATTCAACCACATGTAGTTAAAGATAAAAAGTTTGTAATTGTTATTCCACCA
CCAAATGTTACAGGTAGTTTACATTTAGGACATGCATTAACCAATTCAATTCAAGATGCA
GTTGTTCGTTATCATCGTATGAAGGGTGAAGTTTGTTTATGGGTACCAGGTACAGATCAT
GCAGGTATTGCAACACAAGTAGTTGTAGAGAAAAAGATTTGGAAAGAGNNNNNNNNNN

>VHA717Z.Seq
NNNNNNNNNNAAAGAAACCCTTTACACTTGTATCGATATTGGTCTTCGTTTATTACATCC
ATTCATGCCATACCTCACCGAGGAGTTGTATCAATCATTACCACGTCGTCCAGAAGATGT
CATTCCAACCATTATGTTATGTCCATACCCACAATCTCAATCATGTTGGGCCAATTCTGA
CATTGAGGAAGAAATGAAACAATGTCAAGATATCATCAAATCAATTCGTTCACTTCGTGC
TACCTATACCATTCCAGCCTCTAAAAAACTTCACACTTATCTTCACGTTAAAAATCAAGA
GAATCTCGAACGTTTCACTAAAAACTCTTCATTCATTCGTGTTTTAGCTTATGCCTCTGA
ACTCGAAGTTTCCATTAGTGATGAATCTCGTCCTGGTTGTATCGTTAATGTTGTCAATGA
AAATGTTTCAACCCTTTTAGATGTTAGAGGATCTGTTGATTTCAATCTTGAAATTGCTCG
TCTCGAAACAAAGAAACAACAATTGGTCAAAAACTATGAAACTTTAGTTTCAAAAACTAC
CATACCTTCTTATGATAAAGTTCCACAAAATATCAGAGATGATAATACTCAAAAAATTAA
AGCTTTAGATGAAGAAATTAATATTACAAACAAAACCATTGAAAGTTTTACAAAATTAAA
TATTTAAAATTATAAATAATAATTAATAATAATAATAAAAAGTTTNATACTAAATT

>VHA717P.Seq
ATTGAAAAAGACATAAATATATAATAAATAATGTCAGAACCAAAACCAACTCAACCAATC
AAAGAGATTGATGAGGAAACTAAAAGAAAGAATGAAGAGAAAAAGAAAGCCAAAGAGGCA
GAGAAAGCAGAGAAATTAGCAAAAATGAAAGAAAAGGAAGCTAAACAAGCAGCAGCAAAA
GAAAAAGAATTACAAAATAAACTTAAAAAAGAACAAGAGAAAAAAGAAAAAGAAGCTGAA
GAAAAAGAAAAACAAGAAGCTGAAGAAAAAATTGTTAGAGAACATATTGAAAAAATTTTA
AAAACCCCAAAGGGTGAAAAGGAAGATGTTTCAAGTTTATTATCATCATATCATCCAACA
GCAGTTGAATCAATTTGGTATGATTATTGGTTAGATAATGGATATTTTTCACCAGAGAAA
CAAATGGAGATTCAACCACATGTAGTTAAAGATAAAAAGTTTGTAATTGTTATTCCACCA
CCAAATGTTACAGGTAGTTTACATTTAGGACATGCATTAACCAATTCAATTCAAGATGCA
GTTGTTCGTTATCATCGTATGAAGGGTGAAGTTTGTTTATGGGTACCAGGTACAGATCAT
GCAGGTATTGCAACACAAGTAGTTGTAGAGAAAAAGATTTGGAAAGAG----------AA
AGAAACCCTTTACACTTGTATCGATATTGGTCTTCGTTTATTACATCCATTCATGCCATA
CCTCACCGAGGAGTTGTATCAATCATTACCACGTCGTCCAGAAGATGTCATTCCAACCAT
TATGTTATGTCCATACCCACAATCTCAATCATGTTGGGCCAATTCTGACATTGAGGAAGA
AATGAAACAATGTCAAGATATCATCAAATCAATTCGTTCACTTCGTGCTACCTATACCAT
TCCAGCCTCTAAAAAACTTCACACTTATCTTCACGTTAAAAATCAAGAGAATCTCGAACG
TTTCACTAAAAACTCTTCATTCATTCGTGTTTTAGCTTATGCCTCTGAACTCGAAGTTTC
CATTAGTGATGAATCTCGTCCTGGTTGTATCGTTAATGTTGTCAATGAAAATGTTTCAAC
CCTTTTAGATGTTAGAGGATCTGTTGATTTCAATCTTGAAATTGCTCGTCTCGAAACAAA
GAAACAACAATTGGTCAAAAACTATGAAACTTTAGTTTCAAAAACTACCATACCTTCTTA
TGATAAAGTTCCACAAAATATCAGAGATGATAATACTCAAAAAATTAAAGCTTTAGATGA
AGAAATTAATATTACAAACAAAACCATTGAAAGTTTTACAAAATTAAATATTTAAAATTA
TAAATAATAATTAATAATAATAATAAAAAGTTTNATACTAAATT