>VHA647 (VHA647Q) /CSM/VH/VHA6-B/VHA647Q.Seq.d/
AAATTATTTTCTCCAAATTTATTTTTAATATACAAATAATATATATATAAAATGGCTACA
CCAGTCCAAGCTTCTACTGTAATTCGTGTTAAACCACATCATTATATTCATGTATTAGAT
AATAATACAAATGTTACAAGAGTTGAAGTTGGACCACAAACATTTACTAGACAAGATCAT
GAACGTGTATTAAATCCAACTCCATTACCAATGATTTTGATCCCACCAAGACAATATTGT
ATTATTGAAAACCCAATTATTAGAGATAAAGAAGGTAAATTAGTTGTTGATAAATTCAAT
CAACCAAAACTCCGTCATGGTGATCAAGAAATTCGTTTCTCTCAAGATCCATTCCCATTA
TATCCAGGTGAAGTTGTCATTGCTCAACCAGTTCCATTACAAGTCATCCAAACCAATAGT
GCACTTCGTTTGAAATGTCTTCGTGATTTCGTTGAAGTCAAAGCTGACAAAACCACAGTC
AGTCACATTGCTGGTGATGAATGGTTATTCGAAGGTCCAGGTACCTATTATCCACGTGGT
TGAAGTTCAAGTTGTTXXXXXXXXXX

klfspnlfliyk*YIYKMATPVQASTVIRVKPHHYIHVLDNNTNVTRVEVGPQTFTRQDH
ERVLNPTPLPMILIPPRQYCIIENPIIRDKEGKLVVDKFNQPKLRHGDQEIRFSQDPFPL
YPGEVVIAQPVPLQVIQTNSALRLKCLRDFVEVKADKTTVSHIAGDEWLFEGPGTYYPRG
*sssc---

Frame A:
klfspnlfliyk*YIYKMATPVQASTVIRVKPHHYIHVLDNNTNVTRVEVGPQTFTRQDH
ERVLNPTPLPMILIPPRQYCIIENPIIRDKEGKLVVDKFNQPKLRHGDQEIRFSQDPFPL
YPGEVVIAQPVPLQVIQTNSALRLKCLRDFVEVKADKTTVSHIAGDEWLFEGPGTYYPRG
*sssc---


Frame B:
nyflqiyf*ytnniyikwlhqsklll*fvlnhiiifmy*iiiqmlqelkldhkhlldkim
nvy*iqlhyq*f*shqdnivllktqlleikkvn*llinsinqnsvmvikkfvslkihshy
iqvklsllnqfhyksskpivhfv*nvfvislkskltkpqsvtllvmngyskvqvpiihvv
evqv---


Frame C:
iifskfifniqiiyi*ngytspsfycnsc*ttslyscir**ykcyks*swttniy*trs*
tciksnsitndfdptktilyy*kpny*r*rr*isc**iqstktpsw*srnsflsrsipii
sr*schcstssitshpnq*ctsfemss*fr*sqs*qnhsqshcw**mvirrsryllstwl
kfkl---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA647 (VHA647Q) /CSM/VH/VHA6-B/VHA647Q.Seq.d/                       1102   0.0
VHJ558 (VHJ558Q) /CSM/VH/VHJ5-C/VHJ558Q.Seq.d/                       1086   0.0
VHI718 (VHI718Q) /CSM/VH/VHI7-A/VHI718Q.Seq.d/                       1086   0.0
AHL354 (AHL354Q) /CSM/AH/AHL3-C/AHL354Q.Seq.d/                       1086   0.0
VHM426 (VHM426Q) /CSM/VH/VHM4-B/VHM426Q.Seq.d/                       1082   0.0
VHG660 (VHG660Q) /CSM/VH/VHG6-C/VHG660Q.Seq.d/                       1076   0.0
SHL153 (SHL153Q) /CSM/SH/SHL1-C/SHL153Q.Seq.d/                       1070   0.0
CHH847 (CHH847Q) /CSM/CH/CHH8-B/CHH847Q.Seq.d/                       1065   0.0
VHG741 (VHG741Q) /CSM/VH/VHG7-B/VHG741Q.Seq.d/                       1059   0.0
CHS384 (CHS384Q) /CSM/CH/CHS3-D/CHS384Q.Seq.d/                       1043   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Z37109|Z37109.1 D.discoideum mRNA for major vault protein B.      957   0.0    2
L08646|L08646.1 Dictyostelium discoideum major vault protein alpha (MVPalpha) mRNA, complete cds.   125   6e-51  3
EC754441|EC754441.1 PPE00010907 Agencourt Biosciences Agen-0020 Non-normalized library cloned into Not I - Eco RV sites of pAGEN-1 Physarum polycephalum cDNA, mRNA sequence.    56   1e-06  2
EC752585|EC752585.1 PPE00010540 Agencourt Biosciences Agen-0020 Non-normalized library cloned into Not I - Eco RV sites of pAGEN-1 Physarum polycephalum cDNA, mRNA sequence.    56   1e-06  2
CV681753|CV681753.1 sjs3-011_F10_sjs3-011F10-T3 SJS Schistosoma japonicum cDNA, mRNA sequence.    48   3e-06  3
CT853294|CT853294.1 Oryza sativa (indica cultivar-group) EST sequence:OSIGCRA201F01.    60   2e-05  2
DN310883|DN310883.1 PL06008B1F06 cDNA from juvenile hermaphodites Schmidtea mediterranea cDNA 5', mRNA sequence.    40   0.002  2
DN310193|DN310193.1 PL06006B2B06 cDNA from juvenile hermaphodites Schmidtea mediterranea cDNA 5', mRNA sequence.    40   0.002  2
DN315660|DN315660.1 PL06021B1E07 cDNA from juvenile hermaphodites Schmidtea mediterranea cDNA 5', mRNA sequence.    40   0.003  2
DN314078|DN314078.1 PL06017A2E02 cDNA from juvenile hermaphodites Schmidtea mediterranea cDNA 5', mRNA sequence.    40   0.003  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54659) RecName: Full=Major vault protein beta;          Short=...   337   1e-91
(Q90405) RecName: Full=Major vault protein; AltName: Full=MVP100...   188   7e-47
BC049344_1(BC049344|pid:none) Danio rerio major vault protein, m...   187   2e-46
(Q62667) RecName: Full=Major vault protein;          Short=MVP; ...   185   6e-46
AK167899_1(AK167899|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   185   8e-46
AK152534_1(AK152534|pid:none) Mus musculus bone marrow macrophag...   185   8e-46
S57723(S57723)lrp protein - human                                     185   8e-46
(Q14764) RecName: Full=Major vault protein;          Short=MVP; ...   185   8e-46
AJ238512_1(AJ238512|pid:none) Homo sapiens MVP gene, partial, ex...   185   8e-46
AK088046_1(AK088046|pid:none) Mus musculus 2 days neonate thymus...   184   1e-45

 psg: 0.83  gvh: 0.28  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHA647 is cyt

>VHA647F.Seq
AAATTATTTTCTCCAAATTTATTTTTAATATACAAATAATATATATATAAAATGGCTACA
CCAGTCCAAGCTTCTACTGTAATTCGTGTTAAACCACATCATTATATTCATGTATTAGAT
AATAATACAAATGTTACAAGAGTTGAAGTTGGACCACAAACATTTACTAGACAAGATCAT
GAACGTGTATTAAATCCAACTCCATTACCAATGATTTTGATCCCACCAAGACAATATTGT
ATTATTGAAAACCCAATTATTAGAGATAAAGAAGGTAAATTAGTTGTTGATAAATTCAAT
CAACCAAAACTCCGTCATGGTGATCAAGAAATTCGTTTCTCTCAAGATCCATTCCCATTA
TATCCAGGTGAAGTTGTCATTGCTCAACCAGTTCCATTACAAGTCATCCAAACCAATAGT
GCACTTCGTTTGAAATGTCTTCGTGATTTCGTTGAAGTCAAAGCTGACAAAACCACAGTC
AGTCACATTGCTGGTGATGAATGGTTATTCGAAGGTCCAGGTACCTATTATCCACGTGGT
TGAAGTTCAAGTTGTTNNNNNNNNNN