>VHA622 (VHA622Q) /CSM/VH/VHA6-A/VHA622Q.Seq.d/
ATTATTTTACCACTCATCAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAA
AGAATAAAAAAAATATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTT
TTAAAAGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCA
AAAGATAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAAT
TTCCAACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATT
GATCAAGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAA
AAGGCAAGAATTAGTTGGCCAGATGGTTTTAAGTATTTTTTTGTANATAACCAAGCTGGT
GATTCAGAATCTGCCAAATCTGGTAAAAXXXXXXXXXXCGCCTGTAATGAAGATGAAAAA
NCCATCACCCACGTTCAAGTTACATGTGGTGGTTGTGAAACCTGTTCATACGCTACCGGT
AAATGTGAACCAGATAGTTCACTTTGTAATGATAACAATATTTGTACCATTGATATTTGT
GTCCATGAAGGTATTCTCGATGGTCTACCACAAGGTAATTGTTCAAATACTCCAGTTGAT
TGTGGTGCCAATGATGAAGATAAATGTAAAACTTGGTCTTGTGATCCAACTAAAGGTGGC
TGTCAATCAACCCCAGTCGTTTGTGAAGATAAAGGTAAATGTTTAGTTGGTACTTGTCAA
CCATCCACTGGTCAATGTGAATACTCTGATAGAGTTTGTGATAATGGTGGTGCTTTCTGT
GTTATTGGCCAATGTGATCAAAGACTTGGTTGTTTAGTTTTCGATAGAGTTTGTTCATCT
GATAATAGTAGATGTGAAGAAGGTGTTTGTGTAAATGGTACTGAATCTGAAGAAGGTCAT
TGTAAATCTGTTAAATATGACCCATTACCATTCAATTGTAATACTGGTGCTGTTGTTTCA
ACTGCTGTTATTGCTGGTTCTACTGTCGCTGGTGCTGTTGCTCTTGGTATTTTCCTTTAT
GGTGGTAAAAAAGGTTATGACTATTGGAAAGACTCAAGAAATATTTCAATGGGCTCATCA
AATAGTAATCCATTGTATGAAGAACAACAAACGGAAGAGGTGTCAACCCAATGTANACGA
TCCAGCGCNAATTAAACAAATTAAAC

iilplikkqqiknkkv*nikrikkiytik*KNKMFIQRTFLKVLTLLSIVTVLVHGQTQP
KDKITLKAVIYDQHKFYNPNFQPKNEGEFVLTKGIVKSDIDQEKRIPVLNSMDANDSINK
KARISWPDGFKYFFVXNQAGDSESAKSGK---

---ACNEDEKXITHVQVTCGGCETCSYATGKCEPDSSLCNDNNICTIDICVHEGILDGLP
QGNCSNTPVDCGANDEDKCKTWSCDPTKGGCQSTPVVCEDKGKCLVGTCQPSTGQCEYSD
RVCDNGGAFCVIGQCDQRLGCLVFDRVCSSDNSRCEEGVCVNGTESEEGHCKSVKYDPLP
FNCNTGAVVSTAVIAGSTVAGAVALGIFLYGGKKGYDYWKDSRNISMGSSNSNPLYEEQQ
TEEVSTQCXRSSAN*tn*

Frame A:
iilplikkqqiknkkv*nikrikkiytik*KNKMFIQRTFLKVLTLLSIVTVLVHGQTQP
KDKITLKAVIYDQHKFYNPNFQPKNEGEFVLTKGIVKSDIDQEKRIPVLNSMDANDSINK
KARISWPDGFKYFFVXNQAGDSESAKSGK---

---rl**r*kxhhprssymwwl*nlfiryr*m*tr*ftl***qylyh*ylcp*rysrwst
tr*lfkyss*lwcq**r*m*nlvl*sn*rwlsinpsrl*r*r*mfswylstihwsm*il*
*sl**wwcflcywpm*sktwlfsfr*slfi****m*rrclckwy*i*rrsl*ic*i*pit
iql*ywcccfnccycwfycrwcccswyfplww*krl*llerlkkyfnglik**siv*rtt
ngrgvnpmxtiqrxlnkln

Frame B:
lfyhssknnk*kirkskilke*kkyiq*nkktkcsfkehf*kfllf*v**qfwsmdklnq
kiklh*rp*fmintsfiiqisnqkmrenlf*lrelsnqilikkkefqf*ivwmpmil*ik
rqelvgqmvlsiflxitklviqnlpnlvk---

---ACNEDEKXITHVQVTCGGCETCSYATGKCEPDSSLCNDNNICTIDICVHEGILDGLP
QGNCSNTPVDCGANDEDKCKTWSCDPTKGGCQSTPVVCEDKGKCLVGTCQPSTGQCEYSD
RVCDNGGAFCVIGQCDQRLGCLVFDRVCSSDNSRCEEGVCVNGTESEEGHCKSVKYDPLP
FNCNTGAVVSTAVIAGSTVAGAVALGIFLYGGKKGYDYWKDSRNISMGSSNSNPLYEEQQ
TEEVSTQCXRSSAN*tn*

Frame C:
yftthqkttnkk*eslky*knkkniynkikkqnvhsknifkssysfeysnsfgpwtnstk
r*nyiegrnl*stqvl*skfptkk*gricfn*gncqiry*srkknssfk*ygcq*fyk*k
gkn*larwf*vffcx*psw*fricqiw*---

---pvmkmkxpsptfklhvvvvkpvhtlpvnvnqivhfvmitifvplifvsmkvfsmvyh
kvivqilqlivvpmmkinvklglviqlkvavnqpqsfvkikvnv*lvlvnhplvnvntli
efvimvvlsvllanvikdlvv*fsiefvhliivdvkkvfv*mvlnlkkvivnllnmthyh
sivilvllfqlllllvllslvllllvfsfmvvkkvmtigktqeifqwahqivihcmknnk
rkrcqpnvxdpaxikqik

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA622 (VHA622Q) /CSM/VH/VHA6-A/VHA622Q.Seq.d/                       2107   0.0
VHQ393 (VHQ393Q) /CSM/VH/VHQ3-D/VHQ393Q.Seq.d/                       1505   0.0
VHN636 (VHN636Q) /CSM/VH/VHN6-B/VHN636Q.Seq.d/                       1487   0.0
VHP613 (VHP613Q) /CSM/VH/VHP6-A/VHP613Q.Seq.d/                       1483   0.0
VHA321 (VHA321Q) /CSM/VH/VHA3-A/VHA321Q.Seq.d/                       1473   0.0
VHH724 (VHH724Q) /CSM/VH/VHH7-A/VHH724Q.Seq.d/                       1459   0.0
VHC809 (VHC809Q) /CSM/VH/VHC8-A/VHC809Q.Seq.d/                       1431   0.0
AHL481 (AHL481Q) /CSM/AH/AHL4-D/AHL481Q.Seq.d/                       1419   0.0
VHI218 (VHI218Q) /CSM/VH/VHI2-A/VHI218Q.Seq.d/                       1409   0.0
VHC841 (VHC841Q) /CSM/VH/VHC8-B/VHC841Q.Seq.d/                       1407   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U67940|U67940.1 Dictyostelium discoideum ORFveg106 mRNA, complete cds.   206   1e-48  1
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    92   3e-19  2
EJ898976|EJ898976.1 1093018479520 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061002740139 3', genomic survey sequence.    38   2.3    2
CT851768|CT851768.1 Oryza sativa (indica cultivar-group) EST sequence:OSIGCSN079I08.    46   2.3    1
CI344920|CI344920.1 Oryza sativa (japonica cultivar-group) cDNA, clone: J02B3034O04M3, 3'end of J023034O04.    46   2.3    1
BI982983|BI982983.1 fu57e12.x1 zebrafish adult brain Danio rerio cDNA clone 5333495 3', mRNA sequence.    46   2.3    1
BI428715|BI428715.1 fr72c02.x1 zebrafish adult brain Danio rerio cDNA clone 4966251 3', mRNA sequence.    46   2.3    1
BI536739|BI536739.1 396094 MARC 4BOV Bos taurus cDNA 5', mRNA sequence.    46   2.3    1
AP008213|AP008213.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 7.    46   2.3    1
CT828696|CT828696.1 Oryza sativa (indica cultivar-group) cDNA clone:OSIGCSN079I08, full  insert sequence.    46   2.3    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                519   e-146
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   255   2e-66
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   252   2e-65
(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                210   9e-53
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   205   2e-51
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                147   1e-33
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                134   9e-30
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    89   4e-16
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    88   5e-16
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...    86   2e-15

 psg: 0.84  gvh: 0.70  alm: 0.27  top: 0.37  tms: 0.13  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: mitochondrial
	 32.0 %: endoplasmic reticulum
	 12.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for VHA622 is mit

>VHA622F.Seq
ATTATTTTACCACTCATCAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAA
AGAATAAAAAAAATATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTT
TTAAAAGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCA
AAAGATAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAAT
TTCCAACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATT
GATCAAGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAA
AAGGCAAGAATTAGTTGGCCAGATGGTTTTAAGTATTTTTTTGTANATAACCAAGCTGGT
GATTCAGAATCTGCCAAATCTGGTAAAANNNNNNNNNN

>VHA622Z.Seq
NNNNNNNNNNCGCCTGTAATGAAGATGAAAAANCCATCACCCACGTTCAAGTTACATGTG
GTGGTTGTGAAACCTGTTCATACGCTACCGGTAAATGTGAACCAGATAGTTCACTTTGTA
ATGATAACAATATTTGTACCATTGATATTTGTGTCCATGAAGGTATTCTCGATGGTCTAC
CACAAGGTAATTGTTCAAATACTCCAGTTGATTGTGGTGCCAATGATGAAGATAAATGTA
AAACTTGGTCTTGTGATCCAACTAAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGAAG
ATAAAGGTAAATGTTTAGTTGGTACTTGTCAACCATCCACTGGTCAATGTGAATACTCTG
ATAGAGTTTGTGATAATGGTGGTGCTTTCTGTGTTATTGGCCAATGTGATCAAAGACTTG
GTTGTTTAGTTTTCGATAGAGTTTGTTCATCTGATAATAGTAGATGTGAAGAAGGTGTTT
GTGTAAATGGTACTGAATCTGAAGAAGGTCATTGTAAATCTGTTAAATATGACCCATTAC
CATTCAATTGTAATACTGGTGCTGTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGTCG
CTGGTGCTGTTGCTCTTGGTATTTTCCTTTATGGTGGTAAAAAAGGTTATGACTATTGGA
AAGACTCAAGAAATATTTCAATGGGCTCATCAAATAGTAATCCATTGTATGAAGAACAAC
AAACGGAAGAGGTGTCAACCCAATGTANACGATCCAGCGCNAATTAAACAAATTAAAC

>VHA622P.Seq
ATTATTTTACCACTCATCAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAA
AGAATAAAAAAAATATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTT
TTAAAAGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCA
AAAGATAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAAT
TTCCAACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATT
GATCAAGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAA
AAGGCAAGAATTAGTTGGCCAGATGGTTTTAAGTATTTTTTTGTANATAACCAAGCTGGT
GATTCAGAATCTGCCAAATCTGGTAAAA----------CGCCTGTAATGAAGATGAAAAA
NCCATCACCCACGTTCAAGTTACATGTGGTGGTTGTGAAACCTGTTCATACGCTACCGGT
AAATGTGAACCAGATAGTTCACTTTGTAATGATAACAATATTTGTACCATTGATATTTGT
GTCCATGAAGGTATTCTCGATGGTCTACCACAAGGTAATTGTTCAAATACTCCAGTTGAT
TGTGGTGCCAATGATGAAGATAAATGTAAAACTTGGTCTTGTGATCCAACTAAAGGTGGC
TGTCAATCAACCCCAGTCGTTTGTGAAGATAAAGGTAAATGTTTAGTTGGTACTTGTCAA
CCATCCACTGGTCAATGTGAATACTCTGATAGAGTTTGTGATAATGGTGGTGCTTTCTGT
GTTATTGGCCAATGTGATCAAAGACTTGGTTGTTTAGTTTTCGATAGAGTTTGTTCATCT
GATAATAGTAGATGTGAAGAAGGTGTTTGTGTAAATGGTACTGAATCTGAAGAAGGTCAT
TGTAAATCTGTTAAATATGACCCATTACCATTCAATTGTAATACTGGTGCTGTTGTTTCA
ACTGCTGTTATTGCTGGTTCTACTGTCGCTGGTGCTGTTGCTCTTGGTATTTTCCTTTAT
GGTGGTAAAAAAGGTTATGACTATTGGAAAGACTCAAGAAATATTTCAATGGGCTCATCA
AATAGTAATCCATTGTATGAAGAACAACAAACGGAAGAGGTGTCAACCCAATGTANACGA
TCCAGCGCNAATTAAACAAATTAAAC