>VHA621 (VHA621Q) /CSM/VH/VHA6-A/VHA621Q.Seq.d/
AATTTTTCTTTCTACCAAAAAAAATAGATAACAAAATTAAAAATAAAATGACAATAACAA
GTCCAGTAATTAAAACGCCACCATTAAATTCAGAGATTAGACTTGAATCAAATTTAACAG
TTGAATCAGGTGATATTGAAATTAATGAAAAAGATATTGTAAATGCATCGGAAGTAATAG
TAATTTTAGATGCAGGATCGCAATATAGTAAAGTGATTGATAGAAGAGTTAGAGAATTAA
ATGTTGCATCAGAAATTCATCCATTAAATATAGATTTATTAGAATTAATTAAGATTAAAT
CAAAATCAGGATCAACTATTAAAGGTATAATTATCTCTGGTGGTCCAGAGAGTGTCTATG
GTGAGAATGCACCAAAATTCGATAAATCATTATTCTCTGAGAAATTAAATTTACCAATAT
TTGGTATTTGTTATGGTATGCAATTAATGAATTATATATTTGGTGGAAAGGTTGAAAGTA
ATTCACAACGTGAAGATGGTGTGCACAATATTGAGATTTTAAAAGATGAAAATCAACAAC
TTGTTTCAAAACTATTTAAAAATTTAAATCAAACCGAGCAAGTTTTATTAACACATGGTG
ATAGTGTTACAAAGATXXXXXXXXXXAGCAATTCGTATCATTTGTGCTGATGAACCTTAT
CTAGTGAATTATGATTTTACAAATAATGTCGTGCAATATTTAGTAACCGGTGAAGCTTCC
TCAGAGTTGGAATCAGAAGTTAAAATTAAAATTGATAAACAATTAACAGAGATGAAATGT
AAACGTCAAGATAAAATTACAATTAAACCAGTTTTATTACCAATTCAAACTGTTGGTGTT
CAAGGTGATGGTAGAACTTATTCTTATTTATTAGGTCTTTATAGTTCAGAGAATTCAACA
ATCGACCAAATTCCTTGGTCTTATATTTTCAATTTAGCTAGAACTATACCAAAGATTTGT
CATAATATAAATAGAGTGGTTTTCATTTTCTCTCAAAATGCTACTAAACATACAAATATT
AAAGTTTCAAATGAACCAGTTAAACATATCACACCAACTCGTTTAACTCCTGATGTTATT
AAACAACTTCAACATGCTGATTCAATAGTTTCAGAACAACTCTATAAATATAATTTAATT
AAATCACTATCCCAAGTTCCCGTTGTATCATTACCAATAGATTTCGGTGTTACAGGTAAT
AGATCAATCGCAATTCGTACTTTTATTACAAATGATTTCATGACTGGTGTTCCTGCGATT
CCTGGTAAAAATATTAGTTTCGATTGTTTACAAGAAATTACAAATAATATTTTAACTTCT
GTAAAGGTATTTCAAAAGTTTTATTNATGACTTCAAAACCACCGGTACTAC

FFFLPKKIDNKIKNKMTITSPVIKTPPLNSEIRLESNLTVESGDIEINEKDIVNASEVIV
ILDAGSQYSKVIDRRVRELNVASEIHPLNIDLLELIKIKSKSGSTIKGIIISGGPESVYG
ENAPKFDKSLFSEKLNLPIFGICYGMQLMNYIFGGKVESNSQREDGVHNIEILKDENQQL
VSKLFKNLNQTEQVLLTHGDSVTK---

---AIRIICADEPYLVNYDFTNNVVQYLVTGEASSELESEVKIKIDKQLTEMKCKRQDKI
TIKPVLLPIQTVGVQGDGRTYSYLLGLYSSENSTIDQIPWSYIFNLARTIPKICHNINRV
VFIFSQNATKHTNIKVSNEPVKHITPTRLTPDVIKQLQHADSIVSEQLYKYNLIKSLSQV
PVVSLPIDFGVTGNRSIAIRTFITNDFMTGVPAIPGKNISFDCLQEITNNILTSVKVFQK
FYX*lqnhryy

Frame A:
nfsfyqkk*itklkik*q*qvq*lkrhh*iqrldlnqi*qlnqvilklmkkil*mhrk**
*f*mqdrnivk*lieelen*mlhqkfih*i*iy*n*lrlnqnqdqllkv*lslvvqrvsm
vrmhqnsinhyslrn*iyqylvfvmvcn**iiylverlkvihnvkmvctilrf*kmkinn
lfqnylki*ikpskfy*hmvivlqr---

---snsyhlc**tlssel*fyk*craifsnr*sflrvgirs*n*n**tinrdem*tsr*n
yn*tsfitnsncwcsr*w*nlflfirsl*frefnnrpnslvlyfqfs*nytkdls*yk*s
gfhflskcy*tyky*sfk*ts*tyhtnsfns*cy*ttstc*fnsfrttl*i*fn*itips
srciitnrfrcyr**inrnsyfyyk*fhdwcscdsw*ky*frlftrnyk*yfnfckgisk
vlxmtskppvl

Frame B:
iflstkknr*qn*k*ndnnkssn*natikfrd*t*ikfns*ir*y*n**kryckcigsns
nfrcriai**sd**ks*rikccirnssikyrfirin*d*ikiriny*rynylwwsreclw
*ectkir*iiil*eikftniwyllwyainelyiwwkg*k*ftt*rwcaqy*dfkr*kstt
cfkti*kfksnrasfintw**cykd---

---AIRIICADEPYLVNYDFTNNVVQYLVTGEASSELESEVKIKIDKQLTEMKCKRQDKI
TIKPVLLPIQTVGVQGDGRTYSYLLGLYSSENSTIDQIPWSYIFNLARTIPKICHNINRV
VFIFSQNATKHTNIKVSNEPVKHITPTRLTPDVIKQLQHADSIVSEQLYKYNLIKSLSQV
PVVSLPIDFGVTGNRSIAIRTFITNDFMTGVPAIPGKNISFDCLQEITNNILTSVKVFQK
FYX*lqnhryy

Frame C:
FFFLPKKIDNKIKNKMTITSPVIKTPPLNSEIRLESNLTVESGDIEINEKDIVNASEVIV
ILDAGSQYSKVIDRRVRELNVASEIHPLNIDLLELIKIKSKSGSTIKGIIISGGPESVYG
ENAPKFDKSLFSEKLNLPIFGICYGMQLMNYIFGGKVESNSQREDGVHNIEILKDENQQL
VSKLFKNLNQTEQVLLTHGDSVTK---

---qfvsfvlmnli**imilqimscni**pvklpqswnqklklklinn*qr*nvnvkikl
qlnqfyyqfkllvfkvmveliliy*vfivqriqqstkflglifsi*lelyqrfvii*iew
fsfslkmllniqilkfqmnqlnishqlv*llmllnnfnmliq*fqnnsinii*lnhypkf
plyhyq*isvlqvidqsqfvlllqmis*lvflrflvkilvsivykklqiif*ll*ryfks
fixdfkttgt

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA621 (VHA621Q) /CSM/VH/VHA6-A/VHA621Q.Seq.d/                       2605   0.0
CHE611 (CHE611Q) /CSM/CH/CHE6-A/CHE611Q.Seq.d/                       1469   0.0
CHF324 (CHF324Q) /CSM/CH/CHF3-A/CHF324Q.Seq.d/                       1439   0.0
VHJ185 (VHJ185Q) /CSM/VH/VHJ1-D/VHJ185Q.Seq.d/                       1396   0.0
SFL409 (SFL409Q) /CSM/SF/SFL4-A/SFL409Q.Seq.d/                       1294   0.0
CFJ333 (CFJ333Q) /CSM/CF/CFJ3-B/CFJ333Q.Seq.d/                       1203   0.0
VFL146 (VFL146Q) /CSM/VF/VFL1-B/VFL146Q.Seq.d/                       1187   0.0
CHE516 (CHE516Q) /CSM/CH/CHE5-A/CHE516Q.Seq.d/                       1174   0.0
VFK690 (VFK690Q) /CSM/VF/VFK6-D/VFK690Q.Seq.d/                       1172   0.0
AFL647 (AFL647Q) /CSM/AF/AFL6-B/AFL647Q.Seq.d/                       1172   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M64282|M64282.1 Dictyostelium discoideum GMP synthetase mRNA, complete cds.  1378   0.0    3
DN295855|DN295855.1 PL04001X1E07 cDNA from sexually mature hermaphodites Schmidtea mediterranea cDNA 5', mRNA sequence.    52   2e-09  2
EK359462|EK359462.1 1095468896421 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005852693 3', genomic survey sequence.    56   2e-05  2
AC159447|AC159447.1 Trypanosoma brucei chromosome 7 clone RPCI93-43M14, complete sequence.    62   4e-05  1
AC159433|AC159433.1 Trypanosoma brucei chromosome 7 clone RPCI93-28I8, complete sequence.    62   4e-05  1
AC159406|AC159406.1 Trypanosoma brucei chromosome 7 clone RPCI93-11L3, complete sequence.    62   4e-05  1
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    36   5e-04  12
EJ336321|EJ336321.1 1092963481199 Global-Ocean-Sampling_GS-28-01-01-1P6-2P0KB marine metagenome genomic clone 1061002318952 3', genomic survey sequence.    52   7e-04  3
EE262750|EE262750.1 H01_H01fm1h1_pDNRf_520626 Myzus persicae, line F001, whole male aphid library Myzus persicae cDNA clone H01_H01fm1h1_pDNRf_520626, mRNA sequence.    42   0.001  2
AF404306|AF404306.1 Rhizophydium sp. 136 mitochondrion, complete genome.    34   0.002  9

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM502240_11(AM502240|pid:none) Leishmania infantum chromosome 22.     143   2e-32
AK049755_1(AK049755|pid:none) Mus musculus 12 days embryo spinal...   143   2e-32
(Q3THK7) RecName: Full=GMP synthase [glutamine-hydrolyzing];    ...   143   2e-32
AK168239_1(AK168239|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   143   2e-32
BC111273_1(BC111273|pid:none) Bos taurus guanine monphosphate sy...   142   3e-32
AK302148_1(AK302148|pid:none) Homo sapiens cDNA FLJ57661 complet...   142   4e-32
AK223399_1(AK223399|pid:none) Homo sapiens mRNA for guanine mono...   141   5e-32
CU570794_4(CU570794|pid:none) Zebrafish DNA sequence from clone ...   141   6e-32
AK146654_1(AK146654|pid:none) Mus musculus 16 days embryo heart ...   139   2e-31
AJ720923_1(AJ720923|pid:none) Gallus gallus mRNA for hypothetica...   135   4e-30

 psg: 0.65  gvh: 0.37  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHA621 is cyt

>VHA621F.Seq
AATTTTTCTTTCTACCAAAAAAAATAGATAACAAAATTAAAAATAAAATGACAATAACAA
GTCCAGTAATTAAAACGCCACCATTAAATTCAGAGATTAGACTTGAATCAAATTTAACAG
TTGAATCAGGTGATATTGAAATTAATGAAAAAGATATTGTAAATGCATCGGAAGTAATAG
TAATTTTAGATGCAGGATCGCAATATAGTAAAGTGATTGATAGAAGAGTTAGAGAATTAA
ATGTTGCATCAGAAATTCATCCATTAAATATAGATTTATTAGAATTAATTAAGATTAAAT
CAAAATCAGGATCAACTATTAAAGGTATAATTATCTCTGGTGGTCCAGAGAGTGTCTATG
GTGAGAATGCACCAAAATTCGATAAATCATTATTCTCTGAGAAATTAAATTTACCAATAT
TTGGTATTTGTTATGGTATGCAATTAATGAATTATATATTTGGTGGAAAGGTTGAAAGTA
ATTCACAACGTGAAGATGGTGTGCACAATATTGAGATTTTAAAAGATGAAAATCAACAAC
TTGTTTCAAAACTATTTAAAAATTTAAATCAAACCGAGCAAGTTTTATTAACACATGGTG
ATAGTGTTACAAAGATNNNNNNNNNN

>VHA621Z.Seq
NNNNNNNNNNAGCAATTCGTATCATTTGTGCTGATGAACCTTATCTAGTGAATTATGATT
TTACAAATAATGTCGTGCAATATTTAGTAACCGGTGAAGCTTCCTCAGAGTTGGAATCAG
AAGTTAAAATTAAAATTGATAAACAATTAACAGAGATGAAATGTAAACGTCAAGATAAAA
TTACAATTAAACCAGTTTTATTACCAATTCAAACTGTTGGTGTTCAAGGTGATGGTAGAA
CTTATTCTTATTTATTAGGTCTTTATAGTTCAGAGAATTCAACAATCGACCAAATTCCTT
GGTCTTATATTTTCAATTTAGCTAGAACTATACCAAAGATTTGTCATAATATAAATAGAG
TGGTTTTCATTTTCTCTCAAAATGCTACTAAACATACAAATATTAAAGTTTCAAATGAAC
CAGTTAAACATATCACACCAACTCGTTTAACTCCTGATGTTATTAAACAACTTCAACATG
CTGATTCAATAGTTTCAGAACAACTCTATAAATATAATTTAATTAAATCACTATCCCAAG
TTCCCGTTGTATCATTACCAATAGATTTCGGTGTTACAGGTAATAGATCAATCGCAATTC
GTACTTTTATTACAAATGATTTCATGACTGGTGTTCCTGCGATTCCTGGTAAAAATATTA
GTTTCGATTGTTTACAAGAAATTACAAATAATATTTTAACTTCTGTAAAGGTATTTCAAA
AGTTTTATTNATGACTTCAAAACCACCGGTACTAC

>VHA621P.Seq
AATTTTTCTTTCTACCAAAAAAAATAGATAACAAAATTAAAAATAAAATGACAATAACAA
GTCCAGTAATTAAAACGCCACCATTAAATTCAGAGATTAGACTTGAATCAAATTTAACAG
TTGAATCAGGTGATATTGAAATTAATGAAAAAGATATTGTAAATGCATCGGAAGTAATAG
TAATTTTAGATGCAGGATCGCAATATAGTAAAGTGATTGATAGAAGAGTTAGAGAATTAA
ATGTTGCATCAGAAATTCATCCATTAAATATAGATTTATTAGAATTAATTAAGATTAAAT
CAAAATCAGGATCAACTATTAAAGGTATAATTATCTCTGGTGGTCCAGAGAGTGTCTATG
GTGAGAATGCACCAAAATTCGATAAATCATTATTCTCTGAGAAATTAAATTTACCAATAT
TTGGTATTTGTTATGGTATGCAATTAATGAATTATATATTTGGTGGAAAGGTTGAAAGTA
ATTCACAACGTGAAGATGGTGTGCACAATATTGAGATTTTAAAAGATGAAAATCAACAAC
TTGTTTCAAAACTATTTAAAAATTTAAATCAAACCGAGCAAGTTTTATTAACACATGGTG
ATAGTGTTACAAAGAT----------AGCAATTCGTATCATTTGTGCTGATGAACCTTAT
CTAGTGAATTATGATTTTACAAATAATGTCGTGCAATATTTAGTAACCGGTGAAGCTTCC
TCAGAGTTGGAATCAGAAGTTAAAATTAAAATTGATAAACAATTAACAGAGATGAAATGT
AAACGTCAAGATAAAATTACAATTAAACCAGTTTTATTACCAATTCAAACTGTTGGTGTT
CAAGGTGATGGTAGAACTTATTCTTATTTATTAGGTCTTTATAGTTCAGAGAATTCAACA
ATCGACCAAATTCCTTGGTCTTATATTTTCAATTTAGCTAGAACTATACCAAAGATTTGT
CATAATATAAATAGAGTGGTTTTCATTTTCTCTCAAAATGCTACTAAACATACAAATATT
AAAGTTTCAAATGAACCAGTTAAACATATCACACCAACTCGTTTAACTCCTGATGTTATT
AAACAACTTCAACATGCTGATTCAATAGTTTCAGAACAACTCTATAAATATAATTTAATT
AAATCACTATCCCAAGTTCCCGTTGTATCATTACCAATAGATTTCGGTGTTACAGGTAAT
AGATCAATCGCAATTCGTACTTTTATTACAAATGATTTCATGACTGGTGTTCCTGCGATT
CCTGGTAAAAATATTAGTTTCGATTGTTTACAAGAAATTACAAATAATATTTTAACTTCT
GTAAAGGTATTTCAAAAGTTTTATTNATGACTTCAAAACCACCGGTACTAC