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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHA513
Library VH
(Link to library)
Clone ID VHA513
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11202-1
Original site URL
Representative seq. ID VHA513P
(Link to Original site)
Representative DNA sequence 
>VHA513 (VHA513Q) /CSM/VH/VHA5-A/VHA513Q.Seq.d/
ATTTAAATTTTATATAAAAACAAAGATGAGTAAACCATCAAACACACCACCTCTTCCAAA
AGATGGGGAGAGAAATATTTTAATCACATCAGCTTTACCATACGTAAATAATGTTCCACA
TCTTGGTAATATTATTGGTTGTGTATTAAGTGCAGATGTATATGCAAGATATTGTCGTTT
AAGAAATTATAATTGTATTTATATTTGTGGTACAGATGAATATGGTACAGCAACAGAAAC
AAAAGCATTATCAGAAGGATGTACACCAAAAGAGATTTGTGATAAATATCATGAAATTCA
TAAAGAGATTTATGAATGGTTTAATATTTCATTTGATAAATTTGGAAGAACCTCAACCAA
TAGTCAAACTGAGATTGCTCAAGATATTTTCAACAAGATTAAAGATAACGGATATACATT
AACTCAAGAGATTGAACAACTTTATTGTGAACAAACTTGTAAGATGTTTTTAGCAGATCG
TTTTGTGGAGGGAACATGTCCACATTGTAAATTTGAGGATGCAAGAGGTGATCAATGTGA
TGGTTGTTCCAAATTATTGAATCCAACCGAATTGATCAACCCACGTTGCAAAGTXXXXXX
XXXXAATCCTCATCAATTTAATCAAACTTTTATCAACCCTCTTAGAACCATACATCCCAT
CACTCACCGATAAAGTCCATCAACAATTAAATGTTGAACCAACTAAATATTCCACCCATT
TCGACATCAATGCAATTCCAGCAGGTCATGAAATTTCAAAGGAAATCTTACCATTGGTAA
AGAAAATCGAAGCCGATGACCTCAAGAAATGGCGTACAAAATTCTCTGGAATCGGACCAG
AATTCCCAATCGATATGAAAATTGCCACAGTCTTGGAGGTAAATGATCATCCATCCGCAG
AGAATTTATATGTAATTAAATTAAGTTTAGGTGGTGATCAAACTAAAACCGCAGTTTCAG
GTATTAAAGCAAACTTTGAAAAATCCCAACTCATTGGTAAGAAATTGGCTGTAGTATTAA
ATTTGAAACCTTCAAAATTCAAAGGTGTTCTCTCTGAAGCAATGATTCTTGTTGCTGATG
ATGGTCAAGCCACTAAAGAAAGTTTATCATTCTTGGTACCATCAAATCCACAATCAATTG
AAGCTGGTTCAAAAATTGCCGGTAAAGGTATGTCAATTAAACCAAAACCAACAATTGATT
ATCAAAAAGAATTCCTTCATTATGATCTCACTTGTATTGATAAAATTATTAGTTATAATA
AATCTCAATTATTTATCAATCAAAATGAACCTTTAGTTTC
sequence update 2002.10.25
Translated Amino Acid sequence 
FKFYIKTKMSKPSNTPPLPKDGERNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRL
RNYNCIYICGTDEYGTATETKALSEGCTPKEICDKYHEIHKEIYEWFNISFDKFGRTSTN
SQTEIAQDIFNKIKDNGYTLTQEIEQLYCEQTCKMFLADRFVEGTCPHCKFEDARGDQCD
GCSKLLNPTELINPRCK---

---ILINLIKLLSTLLEPYIPSLTDKVHQQLNVEPTKYSTHFDINAIPAGHEISKEILPL
VKKIEADDLKKWRTKFSGIGPEFPIDMKIATVLEVNDHPSAENLYVIKLSLGGDQTKTAV
SGIKANFEKSQLIGKKLAVVLNLKPSKFKGVLSEAMILVADDGQATKESLSFLVPSNPQS
IEAGSKIAGKGMSIKPKPTIDYQKEFLHYDLTCIDKIISYNKSQLFINQNEPLV
Translated Amino Acid sequence (All Frames) 
Frame A:
i*ilyknkde*tikhttsskrwgekyfnhisftirk*cstsw*yywlcikcrcickilsf
kkl*lylylwyr*iwysnrnksiirrmytkrdl**is*ns*rdl*mv*yfi**iwknlnq
*sn*dcsryfqqd*r*riyinsrd*ttll*tnl*dvfsrsfcggnmstl*i*gckr*sm*
wlfqiiesnridqptlqs---

---nphqfnqtfinplrtihpithr*spstikc*tn*ifhpfrhqcnssrs*nfkgnlti
gkenrsr*pqemaykilwnrtripnryenchslggk*ssirreficn*ikfrw*sn*nrs
fry*skl*kipthw*eigcsikfetfkiqrcsl*sndscc**wssh*rkfiilgtiksti
n*swfkncr*ryvn*tktnn*lskripsl*shly**ny*l**isiiyqsk*tfsf

Frame B:
FKFYIKTKMSKPSNTPPLPKDGERNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRL
RNYNCIYICGTDEYGTATETKALSEGCTPKEICDKYHEIHKEIYEWFNISFDKFGRTSTN
SQTEIAQDIFNKIKDNGYTLTQEIEQLYCEQTCKMFLADRFVEGTCPHCKFEDARGDQCD
GCSKLLNPTELINPRCK---

---ILINLIKLLSTLLEPYIPSLTDKVHQQLNVEPTKYSTHFDINAIPAGHEISKEILPL
VKKIEADDLKKWRTKFSGIGPEFPIDMKIATVLEVNDHPSAENLYVIKLSLGGDQTKTAV
SGIKANFEKSQLIGKKLAVVLNLKPSKFKGVLSEAMILVADDGQATKESLSFLVPSNPQS
IEAGSKIAGKGMSIKPKPTIDYQKEFLHYDLTCIDKIISYNKSQLFINQNEPLV

Frame C:
lnfi*kqr*vnhqthhlfqkmgreif*shqlyht*imfhilvillvvy*vqmymqdivv*
eiiivfifvvqmnmvqqqkqkhyqkdvhqkrfvinimkfikrfmnglifhlinleepqpi
vklrllkifstrlkitdih*lkrlnnfivnklvrcf*qivlwrehvhivnlrmqevinvm
vvpny*iqpn*sthvak---

---sssi*snfyqps*nhtshhspiksinn*mlnqlnippistsmqfqqvmkfqrksyhw
*rkskpmtsrngvqnslesdqnsqsi*klpqswr*miihpqriym*ln*v*vviklkpqf
qvlkqtlknpnslvrnwl*y*i*nlqnskvfslkq*flllmmvkplkkvyhswyhqihnq
lklvqklpvkvcqlnqnqqliikknsfimislvlikllviinlnylsikmnl*f
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA513 (VHA513Q) /CSM/VH/VHA5-A/VHA513Q.Seq.d/                       2518   0.0
VHK475 (VHK475Q) /CSM/VH/VHK4-D/VHK475Q.Seq.d/                       1364   0.0
VHC493 (VHC493Q) /CSM/VH/VHC4-D/VHC493Q.Seq.d/                       1364   0.0
VHC720 (VHC720Q) /CSM/VH/VHC7-A/VHC720Q.Seq.d/                       1358   0.0
AHB519 (AHB519Q) /CSM/AH/AHB5-A/AHB519Q.Seq.d/                       1354   0.0
VHJ847 (VHJ847Q) /CSM/VH/VHJ8-B/VHJ847Q.Seq.d/                       1342   0.0
VHM434 (VHM434Q) /CSM/VH/VHM4-B/VHM434Q.Seq.d/                       1312   0.0
AHD434 (AHD434Q) /CSM/AH/AHD4-B/AHD434Q.Seq.d/                       1294   0.0
VHM654 (VHM654Q) /CSM/VH/VHM6-C/VHM654Q.Seq.d/                       1289   0.0
VHM217 (VHM217Q) /CSM/VH/VHM2-A/VHM217Q.Seq.d/                       1255   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EE261858|EE261858.1 F09_F09ff2k18_pDNRf_513965 Myzus persicae, line F001, whole female aphid library Myzus persicae cDNA clone F09_F09ff2k18_pDNRf_513965, mRNA sequence.    72   8e-34  3
EC756229|EC756229.1 PPE00003887 Agencourt Biosciences Agen-0020 Non-normalized library cloned into Not I - Eco RV sites of pAGEN-1 Physarum polycephalum cDNA, mRNA sequence.    88   1e-32  4
CN761937|CN761937.1 ID0AAA3BE05RM1 ApMS Acyrthosiphon pisum cDNA clone ID0AAA3BE05 5', mRNA sequence.    80   4e-32  4
DY447875|SYM_HUMAN Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS) >, mRNA sequence.    66   2e-20  5
CN560372|CN560372.1 tag32c12.x1 Hydra EST -Kiel 2 Hydra magnipapillata cDNA 3' similar to SW:SYM_HUMAN P56192 METHIONYL-TRNA SYNTHETASE ;, mRNA sequence.    68   2e-19  5
AR549033|AR549033.1 Sequence 4164 from patent US 6747137.          46   2e-18  6
EL477374|EL477374.1 CHUL5577.b1_A04.ab1 CHU(LMS) puzzle sunflower Helianthus paradoxus cDNA clone CHUL5577, mRNA sequence.    62   3e-17  4
AX489310|AX489310.1 Sequence 6610 from Patent WO02053728.          46   2e-16  6
CN560008|CN560008.1 tag38h07.x1 Hydra EST -Kiel 2 Hydra magnipapillata cDNA 3' similar to SW:SYM_HUMAN P56192 METHIONYL-TRNA SYNTHETASE ;, mRNA sequence.    68   7e-16  3
EH431684|EH431684.1 NPE00000653 Neocallimastix patriciarum ZAP II cDNA library Neocallimastix patriciarum cDNA, mRNA sequence.    46   1e-13  3
dna update 2007. 3.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X95) RecName: Full=Probable methionyl-tRNA synthetase, cytop...   432   e-119
(Q9SVN5) RecName: Full=Probable methionyl-tRNA synthetase;      ...   298   2e-79
CR382139_865(CR382139|pid:none) Debaryomyces hansenii strain CBS...   294   5e-78
CU928166_373(CU928166|pid:none) Kluyveromyces thermotolerans str...   293   7e-78
CP000496_359(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   290   8e-77
CR382132_1215(CR382132|pid:none) Yarrowia lipolytica strain CLIB...   289   1e-76
AP008212_1204(AP008212|pid:none) Oryza sativa (japonica cultivar...   289   2e-76
AF040700_1(AF040700|pid:none) Oryza sativa methionyl-tRNA synthe...   289   2e-76
AE016820_701(AE016820|pid:none) Ashbya gossypii (= Eremothecium ...   288   3e-76
BC077353_1(BC077353|pid:none) Xenopus laevis methionine-tRNA syn...   285   3e-75
protein update 2009. 6.26
PSORT 

 psg: 0.74  gvh: 0.59  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHA513 is cyt
5' end seq. ID VHA513F
5' end seq. 
>VHA513F.Seq
ATTTAAATTTTATATAAAAACAAAGATGAGTAAACCATCAAACACACCACCTCTTCCAAA
AGATGGGGAGAGAAATATTTTAATCACATCAGCTTTACCATACGTAAATAATGTTCCACA
TCTTGGTAATATTATTGGTTGTGTATTAAGTGCAGATGTATATGCAAGATATTGTCGTTT
AAGAAATTATAATTGTATTTATATTTGTGGTACAGATGAATATGGTACAGCAACAGAAAC
AAAAGCATTATCAGAAGGATGTACACCAAAAGAGATTTGTGATAAATATCATGAAATTCA
TAAAGAGATTTATGAATGGTTTAATATTTCATTTGATAAATTTGGAAGAACCTCAACCAA
TAGTCAAACTGAGATTGCTCAAGATATTTTCAACAAGATTAAAGATAACGGATATACATT
AACTCAAGAGATTGAACAACTTTATTGTGAACAAACTTGTAAGATGTTTTTAGCAGATCG
TTTTGTGGAGGGAACATGTCCACATTGTAAATTTGAGGATGCAAGAGGTGATCAATGTGA
TGGTTGTTCCAAATTATTGAATCCAACCGAATTGATCAACCCACGTTGCAAAGTNNNNNN
NNNN
Length of 5' end seq. 604
3' end seq. ID VHA513Z
3' end seq. 
>VHA513Z.Seq
NNNNNNNNNNAATCCTCATCAATTTAATCAAACTTTTATCAACCCTCTTAGAACCATACA
TCCCATCACTCACCGATAAAGTCCATCAACAATTAAATGTTGAACCAACTAAATATTCCA
CCCATTTCGACATCAATGCAATTCCAGCAGGTCATGAAATTTCAAAGGAAATCTTACCAT
TGGTAAAGAAAATCGAAGCCGATGACCTCAAGAAATGGCGTACAAAATTCTCTGGAATCG
GACCAGAATTCCCAATCGATATGAAAATTGCCACAGTCTTGGAGGTAAATGATCATCCAT
CCGCAGAGAATTTATATGTAATTAAATTAAGTTTAGGTGGTGATCAAACTAAAACCGCAG
TTTCAGGTATTAAAGCAAACTTTGAAAAATCCCAACTCATTGGTAAGAAATTGGCTGTAG
TATTAAATTTGAAACCTTCAAAATTCAAAGGTGTTCTCTCTGAAGCAATGATTCTTGTTG
CTGATGATGGTCAAGCCACTAAAGAAAGTTTATCATTCTTGGTACCATCAAATCCACAAT
CAATTGAAGCTGGTTCAAAAATTGCCGGTAAAGGTATGTCAATTAAACCAAAACCAACAA
TTGATTATCAAAAAGAATTCCTTCATTATGATCTCACTTGTATTGATAAAATTATTAGTT
ATAATAAATCTCAATTATTTATCAATCAAAATGAACCTTTAGTTTC
Length of 3' end seq. 706
Connected seq. ID VHA513P
Connected seq. 
>VHA513P.Seq
ATTTAAATTTTATATAAAAACAAAGATGAGTAAACCATCAAACACACCACCTCTTCCAAA
AGATGGGGAGAGAAATATTTTAATCACATCAGCTTTACCATACGTAAATAATGTTCCACA
TCTTGGTAATATTATTGGTTGTGTATTAAGTGCAGATGTATATGCAAGATATTGTCGTTT
AAGAAATTATAATTGTATTTATATTTGTGGTACAGATGAATATGGTACAGCAACAGAAAC
AAAAGCATTATCAGAAGGATGTACACCAAAAGAGATTTGTGATAAATATCATGAAATTCA
TAAAGAGATTTATGAATGGTTTAATATTTCATTTGATAAATTTGGAAGAACCTCAACCAA
TAGTCAAACTGAGATTGCTCAAGATATTTTCAACAAGATTAAAGATAACGGATATACATT
AACTCAAGAGATTGAACAACTTTATTGTGAACAAACTTGTAAGATGTTTTTAGCAGATCG
TTTTGTGGAGGGAACATGTCCACATTGTAAATTTGAGGATGCAAGAGGTGATCAATGTGA
TGGTTGTTCCAAATTATTGAATCCAACCGAATTGATCAACCCACGTTGCAAAGT------
----AATCCTCATCAATTTAATCAAACTTTTATCAACCCTCTTAGAACCATACATCCCAT
CACTCACCGATAAAGTCCATCAACAATTAAATGTTGAACCAACTAAATATTCCACCCATT
TCGACATCAATGCAATTCCAGCAGGTCATGAAATTTCAAAGGAAATCTTACCATTGGTAA
AGAAAATCGAAGCCGATGACCTCAAGAAATGGCGTACAAAATTCTCTGGAATCGGACCAG
AATTCCCAATCGATATGAAAATTGCCACAGTCTTGGAGGTAAATGATCATCCATCCGCAG
AGAATTTATATGTAATTAAATTAAGTTTAGGTGGTGATCAAACTAAAACCGCAGTTTCAG
GTATTAAAGCAAACTTTGAAAAATCCCAACTCATTGGTAAGAAATTGGCTGTAGTATTAA
ATTTGAAACCTTCAAAATTCAAAGGTGTTCTCTCTGAAGCAATGATTCTTGTTGCTGATG
ATGGTCAAGCCACTAAAGAAAGTTTATCATTCTTGGTACCATCAAATCCACAATCAATTG
AAGCTGGTTCAAAAATTGCCGGTAAAGGTATGTCAATTAAACCAAAACCAACAATTGATT
ATCAAAAAGAATTCCTTCATTATGATCTCACTTGTATTGATAAAATTATTAGTTATAATA
AATCTCAATTATTTATCAATCAAAATGAACCTTTAGTTTC
Length of connected seq. 1290
Full length Seq ID -
Full length Seq. -
Length of full length seq. -