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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHA468
Library VH
(Link to library)
Clone ID VHA468
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U13534-1|Contig-U15800-1
Original site URL
Representative seq. ID VHA468P
(Link to Original site)
Representative DNA sequence 
>VHA468 (VHA468Q) /CSM/VH/VHA4-C/VHA468Q.Seq.d/
CCTACTGGATTTTAAAATGTCAACATTTAATATTAGTGATATTGATTGCGGAGCTACTTT
CATCTTATGAAAAAGCAATAGGAGAACAAATACCAATTGAGGATGAAGATCATAGTCCAA
TTGAACAAAAAGCTTATCAAAATATTAAACCAATTGATATTAAAAATGGACCCATCAAAT
TAATATTAGTTGGTGATAGTGGTGTTGGTAAGACTACATTTTTAAAACGTCATATAATAG
GCGAATTTCAAAAAAGCCATATTCCTACGGTTGGAGTAGAACTAAGTAATCTAATTCTTT
ACACTAATATCGGCAATGTTAATTTTGATATTTGGGATACTTGTGGTGATGAAAAATTTG
AAGTATTTAGACATTTATATTATGTTGGAAGTCAATGTGCAATTATTATGTTTGATGTTT
CTTCAAGAGTTTCATATAGGAATGTACCAAGTTGGTTTAATGAATTAACAAAGGAATTAG
GTAATAATATACCAATTATTTTAGTTGGTAATAAAGTTGATATCAAAGATCGTAAAGTTA
AAGCAAAGGAAATTAATTTTCATCGTAGTAAGAATATTCACTATTGTXXXXXXXXXXGGA
TCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCA
TCATCATCATCATCATCATCATCATCATCATCATCAACAGCATCACTATTTGAATTTGGA
CCACCACCAACAGCACCACCATTTGATTTTGGAACACCAAAAACAACAACAGCAGCATCG
CCATCAAGTGCGCAATTTTCATTGGGATTATCATCATCAAAAAATAAACCATCATTACCA
GTAGTTAAAAAATTTGACAATACTAAAGAAATTAAATTAGTATTAATTGGAGATGGTGGA
GTTGGTAAATCCACCTATATAAATAGACTTTTAACAGGTGAATTTGAAACTCAATATGTT
GCAACTTTTGGTTGTAGCGTTCATAAATTTAATTTTAAAACAACTATTGGTGATGTTAAT
TTCAGTATTTGGGATATGGCTTTTACTAGATTACCTGGAGCTATGAGAGATGATTATATG
ACTGGAGCGGAATGTGCAATTATTATGTTTGATGTTTGTTCAAGAGAATCATATAGAAGC
GTTAACGATTGGTTTAATGAATTATTAAGAATAACAGGTAAGGTGGTTCCAATGGTTTTA
GTTGGTAATAAAGTAGAATCAATGGGTAAAAGAATTAACCCAAGTCAGATTATATTTCAT
CGTAAAAAGAATATTTCATATTTTGAACTATCGGTTAGAACAAATTTTAATCTTGAAAAA
CCATTCCTCAAATTAATCAGACCTTTAAGTGGTAATGATAATGTTGACTTAATTCCATCA
CCAGATTATCATTTTCATTTAACANCCAAAGCACAAGAT
sequence update 2002.10.25
Translated Amino Acid sequence 
YWILKCQHLILVILIAELLSSYEKAIGEQIPIEDEDHSPIEQKAYQNIKPIDIKNGPIKL
ILVGDSGVGKTTFLKRHIIGEFQKSHIPTVGVELSNLILYTNIGNVNFDIWDTCGDEKFE
VFRHLYYVGSQCAIIMFDVSSRVSYRNVPSWFNELTKELGNNIPIILVGNKVDIKDRKVK
AKEINFHRSKNIHYC---

---GSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTASLFEFGPPPTAPPFDFGTPKTT
TAASPSSAQFSLGLSSSKNKPSLPVVKKFDNTKEIKLVLIGDGGVGKSTYINRLLTGEFE
TQYVATFGCSVHKFNFKTTIGDVNFSIWDMAFTRLPGAMRDDYMTGAECAIIMFDVCSRE
SYRSVNDWFNELLRITGKVVPMVLVGNKVESMGKRINPSQIIFHRKKNISYFELSVRTNF
NLEKPFLKLIRPLSGNDNVDLIPSPDYHFHLTXKAQD
Translated Amino Acid sequence (All Frames) 
Frame A:
ptgf*nvni*y**y*lrsyfhlmkkq*enkyqlrmkiivqlnkklikilnqlilkmdpsn
*y*lvivvlvrlhf*nvi**anfkkaiflrle*n*vi*fftlisamlilifgilvvmknl
kyldiyimlevnvqllclmflqefhigmyqvglmn*qrn*viiyqlf*lvikliskivkl
kqrklifivvrifti---

---GSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTASLFEFGPPPTAPPFDFGTPKTT
TAASPSSAQFSLGLSSSKNKPSLPVVKKFDNTKEIKLVLIGDGGVGKSTYINRLLTGEFE
TQYVATFGCSVHKFNFKTTIGDVNFSIWDMAFTRLPGAMRDDYMTGAECAIIMFDVCSRE
SYRSVNDWFNELLRITGKVVPMVLVGNKVESMGKRINPSQIIFHRKKNISYFELSVRTNF
NLEKPFLKLIRPLSGNDNVDLIPSPDYHFHLTXKAQD

Frame B:
lldfkmstfnisdidcgatfil*ksnrrtntn*g*rs*sn*tkslsky*tn*y*kwthqi
nisw**wcw*dyifktsynrriskkpysygwsrtk*snslh*yrqc*f*ylgylw**ki*
si*tfilcwksmcnyyv*cffksfi*ectklv**inkgir**ytnyfsw**s*yqrs*s*
skgn*fss**eysll---

---dhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhqqhhylnldhhqqhhhlilehqkqq
qqhrhqvrnfhwdyhhqkinhhyq*lknltilkkln*y*lemvelvnppi*idf*qvnlk
lnmlqllvvafinlilkqllvmlisvfgiwllldylel*emii*lernvqllclmfvqen
hiealtiglmny*e*qvrwfqwf*lvik*nqwvkeltqvrlyfivkrifhilnyrleqil
ilknhssn*sdl*vvmimlt*fhhqiiifi*xpkhk

Frame C:
YWILKCQHLILVILIAELLSSYEKAIGEQIPIEDEDHSPIEQKAYQNIKPIDIKNGPIKL
ILVGDSGVGKTTFLKRHIIGEFQKSHIPTVGVELSNLILYTNIGNVNFDIWDTCGDEKFE
VFRHLYYVGSQCAIIMFDVSSRVSYRNVPSWFNELTKELGNNIPIILVGNKVDIKDRKVK
AKEINFHRSKNIHYC---

---iiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiinsiti*iwtttnstti*fwntknnn
ssiaikcaifigiiiikk*tiitss*ki*qy*rn*isinwrwwsw*ihlyk*tfnr*i*n
siccnfwl*rs*i*f*nnyw*c*fqylgygfy*itwsyer*lydwsgmcnyyv*clfkri
i*kr*rlv**iiknnr*ggsngfsw**sring*kn*pksdyiss*keyfif*tig*nkf*
s*ktipqinqtfkw***c*lnsitrlsfsfnxqstr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA468 (VHA468Q) /CSM/VH/VHA4-C/VHA468Q.Seq.d/                       1427   0.0
VHB702 (VHB702Q) /CSM/VH/VHB7-A/VHB702Q.Seq.d/                       1394   0.0
VSI638 (VSI638Q) /CSM/VS/VSI6-B/VSI638Q.Seq.d/                         52   2e-05
VSI212 (VSI212Q) /CSM/VS/VSI2-A/VSI212Q.Seq.d/                         52   2e-05
VSH423 (VSH423Q) /CSM/VS/VSH4-A/VSH423Q.Seq.d/                         52   2e-05
VSG678 (VSG678Q) /CSM/VS/VSG6-D/VSG678Q.Seq.d/                         52   2e-05
VSG423 (VSG423Q) /CSM/VS/VSG4-A/VSG423Q.Seq.d/                         52   2e-05
VSG244 (VSG244Q) /CSM/VS/VSG2-B/VSG244Q.Seq.d/                         52   2e-05
VSG164 (VSG164Q) /CSM/VS/VSG1-C/VSG164Q.Seq.d/                         52   2e-05
VSF607 (VSF607Q) /CSM/VS/VSF6-A/VSF607Q.Seq.d/                         52   2e-05
own update 2002.12.20
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   454   0.0    11
EH014080|EH014080.1 USDA-FP_186816 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt065_F09 5', mRNA sequence.    62   5e-19  4
EH013163|EH013163.1 USDA-FP_185990 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt055_D06 5', mRNA sequence.    62   6e-19  4
EC825570|EC825570.1 SME00006960 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    56   8e-19  5
EC825841|EC825841.1 SME00002495 esmbsro2 Sawyeria marylandensis cDNA, mRNA sequence.    56   1e-16  5
BP189262|BP189262.1 Dugesia japonica cDNA, clone: 06265_HH, expressed in the head.    74   5e-12  2
BP189128|BP189128.1 Dugesia japonica cDNA, clone: 06086_HH, expressed in the head.    74   5e-12  2
BP187150|BP187150.1 Dugesia japonica cDNA, clone: 00666_HH, expressed in the head.    74   5e-12  2
EH015049|EH015049.1 USDA-FP_187688 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt078_A03 5', mRNA sequence.    62   8e-12  3
EG726098|EG726098.1 GTE00017227 Guillardia theta non-normalised Guillardia theta cDNA, mRNA sequence.    46   2e-11  3
dna update 2007. 3.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116957_29(AC116957|pid:none) Dictyostelium discoideum chromoso...   408   e-112
(P32836) RecName: Full=GTP-binding nuclear protein GSP2/CNR2;  &...   182   3e-44
(Q74ZA9) RecName: Full=GTP-binding nuclear protein GSP1/Ran;  &A...   181   6e-44
CR382121_184(CR382121|pid:none) Kluyveromyces lactis strain NRRL...   181   6e-44
JC1455(JC1455;S20712)GTP-binding protein Ran/TC4 - dog                181   6e-44
(P33519) RecName: Full=GTP-binding nuclear protein Ran; AltName:...   180   1e-43
DQ414544_1(DQ414544|pid:none) Suberites domuncula Ran gene, comp...   179   2e-43
(Q6FR65) RecName: Full=GTP-binding nuclear protein GSP1/Ran;  &C...   179   2e-43
EF103373_1(EF103373|pid:none) Haliotis discus discus Ran-1-prov ...   179   2e-43
(P38545) RecName: Full=GTP-binding nuclear protein Ran; AltName:...   178   4e-43
protein update 2009. 6.26
PSORT 

 psg: 0.98  gvh: 0.64  alm: 0.28  top: 0.23  tms: 0.07  mit: 0.26  mip: 0.00
 nuc: 0.02  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 1.67  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 1.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: Golgi
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for VHA468 is end
5' end seq. ID VHA468F
5' end seq. 
>VHA468F.Seq
CCTACTGGATTTTAAAATGTCAACATTTAATATTAGTGATATTGATTGCGGAGCTACTTT
CATCTTATGAAAAAGCAATAGGAGAACAAATACCAATTGAGGATGAAGATCATAGTCCAA
TTGAACAAAAAGCTTATCAAAATATTAAACCAATTGATATTAAAAATGGACCCATCAAAT
TAATATTAGTTGGTGATAGTGGTGTTGGTAAGACTACATTTTTAAAACGTCATATAATAG
GCGAATTTCAAAAAAGCCATATTCCTACGGTTGGAGTAGAACTAAGTAATCTAATTCTTT
ACACTAATATCGGCAATGTTAATTTTGATATTTGGGATACTTGTGGTGATGAAAAATTTG
AAGTATTTAGACATTTATATTATGTTGGAAGTCAATGTGCAATTATTATGTTTGATGTTT
CTTCAAGAGTTTCATATAGGAATGTACCAAGTTGGTTTAATGAATTAACAAAGGAATTAG
GTAATAATATACCAATTATTTTAGTTGGTAATAAAGTTGATATCAAAGATCGTAAAGTTA
AAGCAAAGGAAATTAATTTTCATCGTAGTAAGAATATTCACTATTGTNNNNNNNNNN
Length of 5' end seq. 597
3' end seq. ID VHA468Z
3' end seq. 
>VHA468Z.Seq
NNNNNNNNNNGGATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATC
ATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCAACAGCATCACT
ATTTGAATTTGGACCACCACCAACAGCACCACCATTTGATTTTGGAACACCAAAAACAAC
AACAGCAGCATCGCCATCAAGTGCGCAATTTTCATTGGGATTATCATCATCAAAAAATAA
ACCATCATTACCAGTAGTTAAAAAATTTGACAATACTAAAGAAATTAAATTAGTATTAAT
TGGAGATGGTGGAGTTGGTAAATCCACCTATATAAATAGACTTTTAACAGGTGAATTTGA
AACTCAATATGTTGCAACTTTTGGTTGTAGCGTTCATAAATTTAATTTTAAAACAACTAT
TGGTGATGTTAATTTCAGTATTTGGGATATGGCTTTTACTAGATTACCTGGAGCTATGAG
AGATGATTATATGACTGGAGCGGAATGTGCAATTATTATGTTTGATGTTTGTTCAAGAGA
ATCATATAGAAGCGTTAACGATTGGTTTAATGAATTATTAAGAATAACAGGTAAGGTGGT
TCCAATGGTTTTAGTTGGTAATAAAGTAGAATCAATGGGTAAAAGAATTAACCCAAGTCA
GATTATATTTCATCGTAAAAAGAATATTTCATATTTTGAACTATCGGTTAGAACAAATTT
TAATCTTGAAAAACCATTCCTCAAATTAATCAGACCTTTAAGTGGTAATGATAATGTTGA
CTTAATTCCATCACCAGATTATCATTTTCATTTAACANCCAAAGCACAAGAT
Length of 3' end seq. 832
Connected seq. ID VHA468P
Connected seq. 
>VHA468P.Seq
CCTACTGGATTTTAAAATGTCAACATTTAATATTAGTGATATTGATTGCGGAGCTACTTT
CATCTTATGAAAAAGCAATAGGAGAACAAATACCAATTGAGGATGAAGATCATAGTCCAA
TTGAACAAAAAGCTTATCAAAATATTAAACCAATTGATATTAAAAATGGACCCATCAAAT
TAATATTAGTTGGTGATAGTGGTGTTGGTAAGACTACATTTTTAAAACGTCATATAATAG
GCGAATTTCAAAAAAGCCATATTCCTACGGTTGGAGTAGAACTAAGTAATCTAATTCTTT
ACACTAATATCGGCAATGTTAATTTTGATATTTGGGATACTTGTGGTGATGAAAAATTTG
AAGTATTTAGACATTTATATTATGTTGGAAGTCAATGTGCAATTATTATGTTTGATGTTT
CTTCAAGAGTTTCATATAGGAATGTACCAAGTTGGTTTAATGAATTAACAAAGGAATTAG
GTAATAATATACCAATTATTTTAGTTGGTAATAAAGTTGATATCAAAGATCGTAAAGTTA
AAGCAAAGGAAATTAATTTTCATCGTAGTAAGAATATTCACTATTGT----------GGA
TCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCATCA
TCATCATCATCATCATCATCATCATCATCATCATCAACAGCATCACTATTTGAATTTGGA
CCACCACCAACAGCACCACCATTTGATTTTGGAACACCAAAAACAACAACAGCAGCATCG
CCATCAAGTGCGCAATTTTCATTGGGATTATCATCATCAAAAAATAAACCATCATTACCA
GTAGTTAAAAAATTTGACAATACTAAAGAAATTAAATTAGTATTAATTGGAGATGGTGGA
GTTGGTAAATCCACCTATATAAATAGACTTTTAACAGGTGAATTTGAAACTCAATATGTT
GCAACTTTTGGTTGTAGCGTTCATAAATTTAATTTTAAAACAACTATTGGTGATGTTAAT
TTCAGTATTTGGGATATGGCTTTTACTAGATTACCTGGAGCTATGAGAGATGATTATATG
ACTGGAGCGGAATGTGCAATTATTATGTTTGATGTTTGTTCAAGAGAATCATATAGAAGC
GTTAACGATTGGTTTAATGAATTATTAAGAATAACAGGTAAGGTGGTTCCAATGGTTTTA
GTTGGTAATAAAGTAGAATCAATGGGTAAAAGAATTAACCCAAGTCAGATTATATTTCAT
CGTAAAAAGAATATTTCATATTTTGAACTATCGGTTAGAACAAATTTTAATCTTGAAAAA
CCATTCCTCAAATTAATCAGACCTTTAAGTGGTAATGATAATGTTGACTTAATTCCATCA
CCAGATTATCATTTTCATTTAACANCCAAAGCACAAGAT
Length of connected seq. 1409
Full length Seq ID -
Full length Seq. -
Length of full length seq. -