>VHA467 (VHA467Q) /CSM/VH/VHA4-C/VHA467Q.Seq.d/
ATCATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTC
ATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCG
AAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCAAATT
ATGTTCACXXXXXXXXXXCAATCGATGAAGCCGTTGAATTGGCTCAATCATCAGATGTTG
TTGTCGTTGTAATTGGTGAACTTCCCAGAAGCTGAAACTCCAGGTGATATTTATGATCTC
TCTATGGATCCAAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTA
GTTTTAATTTTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGT
GCTGCAGTTTTAATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATT
CTCATGGGTAATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGT
GATATCGGTGTTCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTC
CAATTTGGTGATGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAAT
TGTAAACCAATCTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACT
TTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATAT
CTCTCTGATCTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAA
GTTGATCTTATGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTC
TCCTTTATTGGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTAGGTGGA
ATCAACAATTAGGGTTTA

HFNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKP
RTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITT
LTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIVIE
GSPNKIPMIYGLDSVHGANYVH---

---IDEAVELAQSSDVVVVVIGELPRS*nsr*yl*slygsk*stlittig*hw*tssfnf
g*sssknfttrsrlqlccsfnglspr**rw*tnrqhshg*cksiwsssfnlsryyw*yrc
silpqiq*kwchhstlpiw*wsflyhf*lyqsrl*ql*tnlwsiw*lhwcfrsilyfhch
chkqw*rsr*rlcslisl*slgssysrs*nvkrfpks*syas*istnlfypqcl*illyw
cr**nyfrigqfiirwnqqlgf

Frame A:
iismni*lnsilyiyikr*kllkvyfychc***iy**vqhmevqle*allevkklkslkn
hvlleikensn*sivkfiqrnn*ikkilisclpeilllii*cqkcqslkklvk*lnwilq
h*hhqiqlqlmkqh*himlkliildhi*ihqyqvv*qvifiilivvfg*i*lilfkplls
kvvqirfq*fmv*iqymvqimf---

---qsmkplnwlnhqmllsl*lvnfpeaetpgdiydlsmdpnevlllqqlvdtgkpvvli
lvearprilppdlvyscaavlmaylpgseggkpianilmgnvnpsgrlpltypgttgdig
vpyyhkysengvttplfqfgdglsyttfnytnlacsnckpisgqsgnytgvlgqsytftv
tvtnngnvqgkdsvllylsdlwaqvtpevkmlrgfqkvdlmpaksqqisftlnayefsfi
gvdnkitlesvnsslgginn*gl

Frame B:
sfq*ifn*iqfyiyi*kdeny*kfiftvivdsefinkfniwkfn*skhcwr*rs*sh*kt
tyfwk*krtqirv*snlsketiksrky*fhvcqryfc**fnvknvnh*knwsndsigyyn
initkynyn**nnisilc*nllywiifkftsirwfsr*yssy***clvryd*yysnhcyr
r*sk*dsndlwfrfstwcklcs---

---nr*sr*igsiircccrcnw*tsqklklqvifmislwiqmkysyynnwltlvnq*f*f
wlklvqefyhqisstvvlqf*wlisqvvkvvnqsptfswvm*ihlvvfl*liqvllvisv
fhittntvkmvsplhssnlvmvfliplltipispvaivnqslvnlvttlvf*vnpilsls
lsqtmvtfkvktlfsyislifglkllqklkc*evskklilcqlnlnksllpsmpmnspll
vsiikll*nrsihh*vestirv

Frame C:
HFNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKP
RTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITT
LTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIVIE
GSPNKIPMIYGLDSVHGANYVH---

---IDEAVELAQSSDVVVVVIGELPRS*nsr*yl*slygsk*stlittig*hw*tssfnf
g*sssknfttrsrlqlccsfnglspr**rw*tnrqhshg*cksiwsssfnlsryyw*yrc
silpqiq*kwchhstlpiw*wsflyhf*lyqsrl*ql*tnlwsiw*lhwcfrsilyfhch
chkqw*rsr*rlcslisl*slgssysrs*nvkrfpks*syas*istnlfypqcl*illyw
cr**nyfrigqfiirwnqqlgf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA467 (VHA467Q) /CSM/VH/VHA4-C/VHA467Q.Seq.d/                       2617   0.0
VHN466 (VHN466Q) /CSM/VH/VHN4-C/VHN466Q.Seq.d/                       1520   0.0
VHN170 (VHN170Q) /CSM/VH/VHN1-C/VHN170Q.Seq.d/                       1520   0.0
VHJ810 (VHJ810Q) /CSM/VH/VHJ8-A/VHJ810Q.Seq.d/                       1518   0.0
CHL451 (CHL451Q) /CSM/CH/CHL4-C/CHL451Q.Seq.d/                       1518   0.0
VHM890 (VHM890Q) /CSM/VH/VHM8-D/VHM890Q.Seq.d/                       1516   0.0
VHP578 (VHP578Q) /CSM/VH/VHP5-D/VHP578Q.Seq.d/                       1511   0.0
CHR795 (CHR795Q) /CSM/CH/CHR7-D/CHR795Q.Seq.d/                       1509   0.0
VHH715 (VHH715Q) /CSM/VH/VHH7-A/VHH715Q.Seq.d/                       1505   0.0
VHA583 (VHA583Q) /CSM/VH/VHA5-D/VHA583Q.Seq.d/                       1505   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L21014|L21014.1 Dictyostelium discoideum beta-glucosidase mRNA, complete cds.  1110   0.0    2
EH013950|EH013950.1 USDA-FP_186699 Lysiphlebus testaceipes adult whole body Lysiphlebus testaceipes cDNA clone WLt064_C10 5', mRNA sequence.    40   0.043  2
BX293980|BX293980.2 Mycoplasma mycoides subsp. mycoides SC str. PG1, complete genome.    36   0.14   16
BG139849|BG139849.1 EST480291 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP14B10 5' sequence, mRNA sequence.    48   0.15   2
CN215175|CN215175.1 28949 Suspension culture Solanum tuberosum cDNA, mRNA sequence.    40   0.35   3
AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    38   0.63   8
BQ969550|BQ969550.1 QHB38N02.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB38N02, mRNA sequence.    48   0.66   1
AC073286|AC073286.5 Homo sapiens chromosome 3 clone RP11-298M2, *** SEQUENCING IN PROGRESS ***, 47 unordered pieces.    48   0.66   1
AC067915|AC067915.2 Homo sapiens clone RP11-131G15, WORKING DRAFT SEQUENCE, 21 unordered pieces.    48   0.66   1
AC022656|AC022656.3 Homo sapiens clone RP11-28P11, WORKING DRAFT SEQUENCE, 22 unordered pieces.    48   0.66   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   430   e-125
CP000159_2777(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   158   1e-38
CP000383_3519(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   147   9e-34
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...   146   2e-33
CP000383_2243(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   141   5e-32
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   134   6e-30
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...   120   5e-28
CP001251_792(CP001251|pid:none) Dictyoglomus turgidum DSM 6724, ...   118   2e-27
CP000685_3497(CP000685|pid:none) Flavobacterium johnsoniae UW101...   122   3e-27
CP001146_680(CP001146|pid:none) Dictyoglomus thermophilum H-6-12...   119   4e-27

 psg: 0.74  gvh: 0.73  alm: 0.26  top: 0.07  tms: 0.07  mit: 0.34  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.75  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHA467 is cyt

>VHA467F.Seq
ATCATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTC
ATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCG
AAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCAAATT
ATGTTCACNNNNNNNNNN

>VHA467Z.Seq
NNNNNNNNNNCAATCGATGAAGCCGTTGAATTGGCTCAATCATCAGATGTTGTTGTCGTT
GTAATTGGTGAACTTCCCAGAAGCTGAAACTCCAGGTGATATTTATGATCTCTCTATGGA
TCCAAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAAT
TTTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGT
TTTAATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGG
TAATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGG
TGTTCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGG
TGATGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACC
AATCTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGT
CACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGA
TCTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCT
TATGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTAT
TGGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTAGGTGGAATCAACAA
TTAGGGTTTA

>VHA467P.Seq
ATCATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGATATTC
ATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTATCG
AAGGTAGTCCAAATAAGATTCCAATGATTTATGGTTTAGATTCAGTACATGGTGCAAATT
ATGTTCAC----------CAATCGATGAAGCCGTTGAATTGGCTCAATCATCAGATGTTG
TTGTCGTTGTAATTGGTGAACTTCCCAGAAGCTGAAACTCCAGGTGATATTTATGATCTC
TCTATGGATCCAAATGAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTA
GTTTTAATTTTGGTTGAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGT
GCTGCAGTTTTAATGGCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATT
CTCATGGGTAATGTAAATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGT
GATATCGGTGTTCCATATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTC
CAATTTGGTGATGGTCTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAAT
TGTAAACCAATCTCTGGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACT
TTCACTGTCACTGTCACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATAT
CTCTCTGATCTTTGGGCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAA
GTTGATCTTATGCCAGCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTC
TCCTTTATTGGTGTCGATAATAAAATTACTTTAGAATCGGTCAATTCATCATTAGGTGGA
ATCAACAATTAGGGTTTA