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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHA466
Library VH
(Link to library)
Clone ID VHA466
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHA466P
(Link to Original site)
Representative DNA sequence 
>VHA466 (VHA466Q) /CSM/VH/VHA4-C/VHA466Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTACCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAXXXXXXXXXXGATTTANGTTNCCNCNNTGCCGGTCAAGNTTTNCCNCAAN
GNGTTTTNGATCANTGGGNTTCAANTGGNGTTGTCAACAAAAATAAAAAANCCNCTGAAG
TTGCTTTANCCCCCAAAAAAAAAAAANGTTTAGCCCCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---DLXXXXAGQXXPQXVXDXWXSXGVVNKNKKXXEVALXPKKKKXLAP
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kkltslsvellnpsmsllvtlki---

---DLXXXXAGQXXPQXVXDXWXSXGVVNKNKKXXEVALXPKKKKXLAP

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslplfp*sy*irqchcw*h*r*---

---ixvxxxpvkxxxxxfxixgxqxxlstkikxplkllxppkkkxv*pq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---fxxpxcrsxfxxxxfxsxgfxwxcqqk*kxx*scfxpqkkkxfsp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA466 (VHA466Q) /CSM/VH/VHA4-C/VHA466Q.Seq.d/                       1195   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1162   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1162   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1162   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1162   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1162   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1162   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1162   0.0
VHQ438 (VHQ438Q) /CSM/VH/VHQ4-B/VHQ438Q.Seq.d/                       1162   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1160   0.0    1
CX155337|CX155337.1 TfEST072F03 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   5e-34  4
CX154324|CX154324.1 TfEST033C12 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   8e-34  4
CX154971|CX154971.1 TfEST089A07 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   2e-33  4
CX154356|CX154356.1 TfEST029E10 Tf30924 cDNA Library Tritrichomonas foetus cDNA 5', mRNA sequence.    90   3e-33  4
DY889811|DY889811.1 CeleSEQ6761 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6761, mRNA sequence.    84   1e-32  4
DY889772|DY889772.1 CeleSEQ11980 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ11980, mRNA sequence.    84   1e-32  4
DY890935|DY890935.1 CeleSEQ7425 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ7425, mRNA sequence.    84   2e-32  4
DY887441|DY887441.1 CeleSEQ3766 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ3766, mRNA sequence.    84   2e-32  4
DY888936|DY888936.1 CeleSEQ6366 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ6366, mRNA sequence.    84   3e-32  4
dna update 2007. 3.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   265   1e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   260   3e-68
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   259   6e-68
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   259   8e-68
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   2e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   257   3e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   7e-67
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   256   7e-67
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   256   7e-67
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   255   9e-67
protein update 2009. 6.26
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHA466 is cyt
5' end seq. ID VHA466F
5' end seq. 
>VHA466F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTACCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAANNNNNNNNNN
Length of 5' end seq. 620
3' end seq. ID VHA466Z
3' end seq. 
>VHA466Z.Seq
NNNNNNNNNNGATTTANGTTNCCNCNNTGCCGGTCAAGNTTTNCCNCAANGNGTTTTNGA
TCANTGGGNTTCAANTGGNGTTGTCAACAAAAATAAAAAANCCNCTGAAGTTGCTTTANC
CCCCAAAAAAAAAAAANGTTTAGCCCCAA
Length of 3' end seq. 149
Connected seq. ID VHA466P
Connected seq. 
>VHA466P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTACCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAA----------GATTTANGTTNCCNCNNTGCCGGTCAAGNTTTNCCNCAAN
GNGTTTTNGATCANTGGGNTTCAANTGGNGTTGTCAACAAAAATAAAAAANCCNCTGAAG
TTGCTTTANCCCCCAAAAAAAAAAAANGTTTAGCCCCAA
Length of connected seq. 749
Full length Seq ID -
Full length Seq. -
Length of full length seq. -