>VHA421 (VHA421Q) /CSM/VH/VHA4-A/VHA421Q.Seq.d/
ATNCTAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAAGAATAAAAAAAAT
ATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTTTTAAAAGTTCTTAC
TCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCAAAAGATAAAATTAC
ATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAATTTCCAACCAAAAAA
TGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATTGATCAAGAAAAAAG
AATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAAAAGGCAGGAATTAG
TTGGCCAGATGGTTTTAAGTATTTTTTTGTANATAACCAAGCTGGTGATTCANAATCTGC
CAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAATTGGAATGGAGAAGC
ATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAAGGTTTCAATGATGTAAG
CTAXXXXXXXXXXGTTCATACGCTACCGGTAAATGTGAACCAGATAGTTCACTTTGTAAT
GATAACAATATTTGTACCATTGATATTTGTGTCCATGAAGGTATTCTCGATGGTCTACCA
CAAGGTAATTGTTCAAATACTCCAGTTGATTGTGGTGCCAATGATGAAGATAAATGTAAA
ACTTGGTCTTGTGATCCAACTAAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGAAGAT
AAAGGTAAATGTTTAGTTGGTACTTGTCAACCATCCACTGGTCAATGTGAATACTCTGAT
AGAGTTTGTGATAATGGTGGTGCTTTCTGTGTTATTGGCCAATGTGATCAAAGACTTGGT
TGTTTAGTTTTCGATAGAGTTTGTTCATCTGATAATAGTAGATGTGAAGAAGGTGTTTGT
GTAAATGGTACTGAATCTGAAGAAGGTCATTGTAAATCTGTTAAATATGACCCATTACCA
TTCAATTGTAATACTGGTGCTGTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGTCGCT
GGTGCTGTTGCTCTTGGTATTTTCCTTTATGGTGGTAAAAAAGGTTATGACTATTGGAAA
GACTCAAGAAATATTTCAATGGGCTCATCAAATAGTAATCCATTGTATGAAGAACAACAA
ACGGAAGAGGTGTCAACCCAAT

x*kqqiknkkv*nikrikkiytik*KNKMFIQRTFLKVLTLLSIVTVLVHGQTQPKDKIT
LKAVIYDQHKFYNPNFQPKNEGEFVLTKGIVKSDIDQEKRIPVLNSMDANDSINKKAGIS
WPDGFKYFFVXNQAGDSXSAKSGKNLPIQRDIELNWNGEAYEYSNSNYFPINGQGFNDVS
---

---SYATGKCEPDSSLCNDNNICTIDICVHEGILDGLPQGNCSNTPVDCGANDEDKCKTW
SCDPTKGGCQSTPVVCEDKGKCLVGTCQPSTGQCEYSDRVCDNGGAFCVIGQCDQRLGCL
VFDRVCSSDNSRCEEGVCVNGTESEEGHCKSVKYDPLPFNCNTGAVVSTAVIAGSTVAGA
VALGIFLYGGKKGYDYWKDSRNISMGSSNSNPLYEEQQTEEVSTQ

Frame A:
xlkttnkk*eslky*knkkniynkikkqnvhsknifkssysfeysnsfgpwtnstkr*ny
iegrnl*stqvl*skfptkk*gricfn*gncqiry*srkknssfk*ygcq*fyk*kgrn*
larwf*vffcx*psw*fxicqiw*kssnskryrvklewrsi*i**fkllpn*rtrfq*ck
l---

---vhtlpvnvnqivhfvmitifvplifvsmkvfsmvyhkvivqilqlivvpmmkinvkl
glviqlkvavnqpqsfvkikvnv*lvlvnhplvnvntliefvimvvlsvllanvikdlvv
*fsiefvhliivdvkkvfv*mvlnlkkvivnllnmthyhsivilvllfqlllllvllslv
llllvfsfmvvkkvmtigktqeifqwahqivihcmknnkrkrcqpn

Frame B:
x*kqqiknkkv*nikrikkiytik*KNKMFIQRTFLKVLTLLSIVTVLVHGQTQPKDKIT
LKAVIYDQHKFYNPNFQPKNEGEFVLTKGIVKSDIDQEKRIPVLNSMDANDSINKKAGIS
WPDGFKYFFVXNQAGDSXSAKSGKNLPIQRDIELNWNGEAYEYSNSNYFPINGQGFNDVS
---

---firyr*m*tr*ftl***qylyh*ylcp*rysrwsttr*lfkyss*lwcq**r*m*nl
vl*sn*rwlsinpsrl*r*r*mfswylstihwsm*il**sl**wwcflcywpm*sktwlf
sfr*slfi****m*rrclckwy*i*rrsl*ic*i*pitiql*ywcccfnccycwfycrwc
ccswyfplww*krl*llerlkkyfnglik**siv*rttngrgvnp

Frame C:
xknnk*kirkskilke*kkyiq*nkktkcsfkehf*kfllf*v**qfwsmdklnqkiklh
*rp*fmintsfiiqisnqkmrenlf*lrelsnqilikkkefqf*ivwmpmil*ikrqelv
gqmvlsiflxitklvixnlpnlvkifqfkeiss*igmekhmniviqitsqltdkvsmm*a
---

---SYATGKCEPDSSLCNDNNICTIDICVHEGILDGLPQGNCSNTPVDCGANDEDKCKTW
SCDPTKGGCQSTPVVCEDKGKCLVGTCQPSTGQCEYSDRVCDNGGAFCVIGQCDQRLGCL
VFDRVCSSDNSRCEEGVCVNGTESEEGHCKSVKYDPLPFNCNTGAVVSTAVIAGSTVAGA
VALGIFLYGGKKGYDYWKDSRNISMGSSNSNPLYEEQQTEEVSTQ

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA421 (VHA421Q) /CSM/VH/VHA4-A/VHA421Q.Seq.d/                       2139   0.0
VHQ393 (VHQ393Q) /CSM/VH/VHQ3-D/VHQ393Q.Seq.d/                       1326   0.0
VHP613 (VHP613Q) /CSM/VH/VHP6-A/VHP613Q.Seq.d/                       1326   0.0
VHJ737 (VHJ737Q) /CSM/VH/VHJ7-B/VHJ737Q.Seq.d/                       1326   0.0
VHI218 (VHI218Q) /CSM/VH/VHI2-A/VHI218Q.Seq.d/                       1326   0.0
VHA622 (VHA622Q) /CSM/VH/VHA6-A/VHA622Q.Seq.d/                       1326   0.0
AHL481 (AHL481Q) /CSM/AH/AHL4-D/AHL481Q.Seq.d/                       1326   0.0
VHF730 (VHF730Q) /CSM/VH/VHF7-B/VHF730Q.Seq.d/                       1320   0.0
VHN636 (VHN636Q) /CSM/VH/VHN6-B/VHN636Q.Seq.d/                       1318   0.0
VHC809 (VHC809Q) /CSM/VH/VHC8-A/VHC809Q.Seq.d/                       1318   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U67940|U67940.1 Dictyostelium discoideum ORFveg106 mRNA, complete cds.   206   1e-48  1
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    92   3e-19  2
EJ898976|EJ898976.1 1093018479520 Global-Ocean-Sampling_GS-30-02-01-1P0-1P3KB marine metagenome genomic clone 1061002740139 3', genomic survey sequence.    38   2.3    2
CT851768|CT851768.1 Oryza sativa (indica cultivar-group) EST sequence:OSIGCSN079I08.    46   2.3    1
CI344920|CI344920.1 Oryza sativa (japonica cultivar-group) cDNA, clone: J02B3034O04M3, 3'end of J023034O04.    46   2.3    1
BI982983|BI982983.1 fu57e12.x1 zebrafish adult brain Danio rerio cDNA clone 5333495 3', mRNA sequence.    46   2.3    1
BI428715|BI428715.1 fr72c02.x1 zebrafish adult brain Danio rerio cDNA clone 4966251 3', mRNA sequence.    46   2.3    1
BI536739|BI536739.1 396094 MARC 4BOV Bos taurus cDNA 5', mRNA sequence.    46   2.3    1
AP008213|AP008213.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 7.    46   2.3    1
CT828696|CT828696.1 Oryza sativa (indica cultivar-group) cDNA clone:OSIGCSN079I08, full  insert sequence.    46   2.3    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                472   e-131
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   241   4e-62
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   235   2e-60
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   193   1e-47
(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                190   1e-46
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                144   8e-33
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                132   3e-29
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    85   5e-15
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...    82   3e-14
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    78   7e-13

 psg: 0.84  gvh: 0.70  alm: 0.27  top: 0.37  tms: 0.13  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: mitochondrial
	 32.0 %: endoplasmic reticulum
	 12.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for VHA421 is mit

>VHA421F.Seq
ATNCTAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAAGAATAAAAAAAAT
ATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTTTTAAAAGTTCTTAC
TCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCAAAAGATAAAATTAC
ATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAATTTCCAACCAAAAAA
TGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATTGATCAAGAAAAAAG
AATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAAAAGGCAGGAATTAG
TTGGCCAGATGGTTTTAAGTATTTTTTTGTANATAACCAAGCTGGTGATTCANAATCTGC
CAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAATTGGAATGGAGAAGC
ATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAAGGTTTCAATGATGTAAG
CTANNNNNNNNNN

>VHA421Z.Seq
NNNNNNNNNNGTTCATACGCTACCGGTAAATGTGAACCAGATAGTTCACTTTGTAATGAT
AACAATATTTGTACCATTGATATTTGTGTCCATGAAGGTATTCTCGATGGTCTACCACAA
GGTAATTGTTCAAATACTCCAGTTGATTGTGGTGCCAATGATGAAGATAAATGTAAAACT
TGGTCTTGTGATCCAACTAAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGAAGATAAA
GGTAAATGTTTAGTTGGTACTTGTCAACCATCCACTGGTCAATGTGAATACTCTGATAGA
GTTTGTGATAATGGTGGTGCTTTCTGTGTTATTGGCCAATGTGATCAAAGACTTGGTTGT
TTAGTTTTCGATAGAGTTTGTTCATCTGATAATAGTAGATGTGAAGAAGGTGTTTGTGTA
AATGGTACTGAATCTGAAGAAGGTCATTGTAAATCTGTTAAATATGACCCATTACCATTC
AATTGTAATACTGGTGCTGTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGTCGCTGGT
GCTGTTGCTCTTGGTATTTTCCTTTATGGTGGTAAAAAAGGTTATGACTATTGGAAAGAC
TCAAGAAATATTTCAATGGGCTCATCAAATAGTAATCCATTGTATGAAGAACAACAAACG
GAAGAGGTGTCAACCCAAT

>VHA421P.Seq
ATNCTAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAAGAATAAAAAAAAT
ATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTTTTAAAAGTTCTTAC
TCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCAAAAGATAAAATTAC
ATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAATTTCCAACCAAAAAA
TGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATTGATCAAGAAAAAAG
AATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAAAAGGCAGGAATTAG
TTGGCCAGATGGTTTTAAGTATTTTTTTGTANATAACCAAGCTGGTGATTCANAATCTGC
CAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAATTGGAATGGAGAAGC
ATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAAGGTTTCAATGATGTAAG
CTA----------GTTCATACGCTACCGGTAAATGTGAACCAGATAGTTCACTTTGTAAT
GATAACAATATTTGTACCATTGATATTTGTGTCCATGAAGGTATTCTCGATGGTCTACCA
CAAGGTAATTGTTCAAATACTCCAGTTGATTGTGGTGCCAATGATGAAGATAAATGTAAA
ACTTGGTCTTGTGATCCAACTAAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGAAGAT
AAAGGTAAATGTTTAGTTGGTACTTGTCAACCATCCACTGGTCAATGTGAATACTCTGAT
AGAGTTTGTGATAATGGTGGTGCTTTCTGTGTTATTGGCCAATGTGATCAAAGACTTGGT
TGTTTAGTTTTCGATAGAGTTTGTTCATCTGATAATAGTAGATGTGAAGAAGGTGTTTGT
GTAAATGGTACTGAATCTGAAGAAGGTCATTGTAAATCTGTTAAATATGACCCATTACCA
TTCAATTGTAATACTGGTGCTGTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGTCGCT
GGTGCTGTTGCTCTTGGTATTTTCCTTTATGGTGGTAAAAAAGGTTATGACTATTGGAAA
GACTCAAGAAATATTTCAATGGGCTCATCAAATAGTAATCCATTGTATGAAGAACAACAA
ACGGAAGAGGTGTCAACCCAAT