>VHA386 (VHA386Q) /CSM/VH/VHA3-D/VHA386Q.Seq.d/
XXXXXXXXXXGTCATCACCAACATTGATATTCAATCTGTTGAACCAGTCGATCAACGTAC
TCGTGATTCACTCCAAAAATCTGTCCAATTGGCCATTGAAATCACCACCAAATCACAAGA
AGCTGCTGCTCGTCATGAAGCTGAACGTCTTGAACAAGGTGCTCGTGGTAGACTCGAACG
TCAAAAGATTCACGATGAAGCTCAAGCAGAGTTAGCTCGTAAAGATTTATTACAACTCCA
AGCTCAATCAGCTGCAGTCGAGTCAACTGGTCAAGCTACCGCTGAAGCTCAAGCTACTGC
TGAAGCTGCTAACATTGCTGCTGAAGCCAATGTTAAACAAGCTGAATTAAAAGCTCAAGC
CACCAAGATTCGTTCAGAATCTGAAATTTCTCTCTTAAAAGCCAAGAGAGAAAATGAACT
CTCATACCAAAAATCAATTGATGAACTCGAACTCACCAAACAATCTGATCTCGCTGAAAT
TGAAGCCTCCAAATTCAAAGCAATCGTTGAATCCATTGGTAGAGATACCCTCAAATCAAT
TGCTTGTGCTGGTAATGAAATGCAAGCTAAATTATTACAAGGTTTGGGTCTTAAATCATT
TATGATCACCGACGGTAAATCACCACTCAACCTTTTCGATACTGCCAACGGTATCATTGG
TAATAACAATCAAATGGTAAACATGTCAAATGCGCCAAACAATCGGTAGAAGAAATTAAA
AAAAAATAAT

---ITNIDIQSVEPVDQRTRDSLQKSVQLAIEITTKSQEAAARHEAERLEQGARGRLERQ
KIHDEAQAELARKDLLQLQAQSAAVESTGQATAEAQATAEAANIAAEANVKQAELKAQAT
KIRSESEISLLKAKRENELSYQKSIDELELTKQSDLAEIEASKFKAIVESIGRDTLKSIA
CAGNEMQAKLLQGLGLKSFMITDGKSPLNLFDTANGIIGNNNQMVNMSNAPNNR*kklkk
nn

Frame A:
---ssptlifnllnqsinvlvihsknlsnwplksppnhkklllvmklnvlnkvlvvdsnv
krftmklkqs*lvkiyynsklnqlqssqlvklplklklllklltlllkpmlnkln*klkp
prfvqnlkfls*kprekmnshtknqlmnsnspnnlislklkppnskqslnplveipsnql
lvlvmkcklnyykvwvlnhl*sptvnhhstfsilptvslvitikw*tcqmrqtigrrn*k
ki


Frame B:
---hhqh*ysic*tsrstys*ftpkicpigh*nhhqitrsccss*s*ts*trcsw*trts
kdsr*sssrvss*rfittpssiscsrvnwssyr*sssyc*sc*hcc*sqc*ts*iksssh
qdsfri*nfslksqerk*tlipkin**trthqti*sr*n*slqiqsnr*ihw*rypqinc
lcw**nas*iitrfgs*iiydhrr*ittqpfrycqryhw**qsngkhvkcakqsveeikk
k*


Frame C:
---ITNIDIQSVEPVDQRTRDSLQKSVQLAIEITTKSQEAAARHEAERLEQGARGRLERQ
KIHDEAQAELARKDLLQLQAQSAAVESTGQATAEAQATAEAANIAAEANVKQAELKAQAT
KIRSESEISLLKAKRENELSYQKSIDELELTKQSDLAEIEASKFKAIVESIGRDTLKSIA
CAGNEMQAKLLQGLGLKSFMITDGKSPLNLFDTANGIIGNNNQMVNMSNAPNNR*kklkk
nn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA386 (VHA386Q) /CSM/VH/VHA3-D/VHA386Q.Seq.d/                       1402   0.0
VHQ344 (VHQ344Q) /CSM/VH/VHQ3-B/VHQ344Q.Seq.d/                       1388   0.0
CHF884 (CHF884Q) /CSM/CH/CHF8-D/CHF884Q.Seq.d/                       1388   0.0
AHE895 (AHE895Q) /CSM/AH/AHE8-D/AHE895Q.Seq.d/                       1388   0.0
AHI488 (AHI488Q) /CSM/AH/AHI4-D/AHI488Q.Seq.d/                       1368   0.0
VHP312 (VHP312Q) /CSM/VH/VHP3-A/VHP312Q.Seq.d/                       1366   0.0
VHH682 (VHH682Q) /CSM/VH/VHH6-D/VHH682Q.Seq.d/                       1366   0.0
AHB516 (AHB516Q) /CSM/AH/AHB5-A/AHB516Q.Seq.d/                       1360   0.0
VHB605 (VHB605Q) /CSM/VH/VHB6-A/VHB605Q.Seq.d/                       1350   0.0
VHO860 (VHO860Q) /CSM/VH/VHO8-C/VHO860Q.Seq.d/                       1336   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L08646|L08646.1 Dictyostelium discoideum major vault protein alpha (MVPalpha) mRNA, complete cds.  1352   0.0    1
Z37109|Z37109.1 D.discoideum mRNA for major vault protein B.      194   1e-76  3
EG016576|EG016576.1 EST01313_0906 Sporophyte cDNA Library Porphyra haitanensis cDNA 5', mRNA sequence.   127   5e-29  2
EC132986|EC132986.1 HVE00006496 Hartmannella vermiformis Regular Large Hartmannella vermiformis cDNA, mRNA sequence.    92   6e-23  2
AT009029|AT009029.1 Clonorchis sinensis mRNA, clone: CSAD-25-B10, 5' EST.   109   1e-19  1
CX835130|AF255664_1 major vault protein [Ictalurus punctatus], mRNA sequence.    60   3e-13  3
CK740021|CK740021.1 USDA-FP_6515 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE64E02 5', mRNA sequence.    52   2e-12  2
DN137794| MVP100 [Torpedo marmorata], mRNA sequence.    52   5e-11  3
CT836653|CT836653.1 Oryza sativa (indica cultivar-group) cDNA clone:OSIGCRA216N21, full  insert sequence.    56   7e-10  3
EC761254|EC761254.1 PSE00001282 rw_mgpallid Polysphondylium pallidum cDNA, mRNA sequence.    62   5e-09  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54659) RecName: Full=Major vault protein beta;          Short=...   220   3e-56
AK167899_1(AK167899|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   211   2e-53
AK088046_1(AK088046|pid:none) Mus musculus 2 days neonate thymus...   211   2e-53
(Q9EQK5) RecName: Full=Major vault protein;          Short=MVP; ...   211   2e-53
(Q62667) RecName: Full=Major vault protein;          Short=MVP; ...   209   5e-53
AK298238_1(AK298238|pid:none) Homo sapiens cDNA FLJ53437 complet...   207   2e-52
AK293302_1(AK293302|pid:none) Homo sapiens cDNA FLJ54588 complet...   207   2e-52
AK302052_1(AK302052|pid:none) Homo sapiens cDNA FLJ53371 complet...   207   2e-52
S57723(S57723)lrp protein - human                                     207   2e-52
(Q14764) RecName: Full=Major vault protein;          Short=MVP; ...   207   2e-52

 psg: 0.75  gvh: 0.37  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHA386 is cyt

>VHA386Z.Seq
NNNNNNNNNNGTCATCACCAACATTGATATTCAATCTGTTGAACCAGTCGATCAACGTAC
TCGTGATTCACTCCAAAAATCTGTCCAATTGGCCATTGAAATCACCACCAAATCACAAGA
AGCTGCTGCTCGTCATGAAGCTGAACGTCTTGAACAAGGTGCTCGTGGTAGACTCGAACG
TCAAAAGATTCACGATGAAGCTCAAGCAGAGTTAGCTCGTAAAGATTTATTACAACTCCA
AGCTCAATCAGCTGCAGTCGAGTCAACTGGTCAAGCTACCGCTGAAGCTCAAGCTACTGC
TGAAGCTGCTAACATTGCTGCTGAAGCCAATGTTAAACAAGCTGAATTAAAAGCTCAAGC
CACCAAGATTCGTTCAGAATCTGAAATTTCTCTCTTAAAAGCCAAGAGAGAAAATGAACT
CTCATACCAAAAATCAATTGATGAACTCGAACTCACCAAACAATCTGATCTCGCTGAAAT
TGAAGCCTCCAAATTCAAAGCAATCGTTGAATCCATTGGTAGAGATACCCTCAAATCAAT
TGCTTGTGCTGGTAATGAAATGCAAGCTAAATTATTACAAGGTTTGGGTCTTAAATCATT
TATGATCACCGACGGTAAATCACCACTCAACCTTTTCGATACTGCCAACGGTATCATTGG
TAATAACAATCAAATGGTAAACATGTCAAATGCGCCAAACAATCGGTAGAAGAAATTAAA
AAAAAATAAT