>VHA358 (VHA358Q) /CSM/VH/VHA3-C/VHA358Q.Seq.d/
XXXXXXXXXXCCATACGATGATGAACATACTCCAAAGGATTCAGTTGTGACTGCTATATT
AACATTTGGTGAAGGTTATCATAACTTCCATCATGAATTCCCAAATGACTATAGAAACGC
TTATAAATTCTATCAATATGATCCAACTAAATGGTTGATCTNTGCAATGTATTATTTAGG
CTTGGCTTATGATTTGAAAACTTTNTCCAAAAATGAAATTGAAAAAGGTATGATTCAAAT
GCACCAAAAACATTTAGACGCAAAGAAACAATCAATNTATTGGGGTATCAATAAAGAAGA
TTTACCATCAATGACAATCGATGAATTTAATAATTTGGTTGAAAAAGAAAATAAAAAGTT
AATGATAATCAACAAAACCGTATTAGATGTCGAATCTTTTGTTAATGATCACCCAGGTGG
TTTAGCTTACATTAAAATGGGTATTGGTAAAGATGCAACTTCAATGTTTACTGGTGAAGT
TTATGCTCATTCAAATGCTGCTAAAAATTTATTATGTCAATTTTCAATTGCTAAAATTGT
TGATAATCATGATAAAAAACAACAATAATCAAAAAAACAAACAACTTT

---YDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPTKWLIXAMYYLGL
AYDLKTXSKNEIEKGMIQMHQKHLDAKKQSXYWGINKEDLPSMTIDEFNNLVEKENKKLM
IINKTVLDVESFVNDHPGGLAYIKMGIGKDATSMFTGEVYAHSNAAKNLLCQFSIAKIVD
NHDKKQQ*skkqtt

Frame A:
---htmmnilqriql*lly*hlvkviitsimnsqmtietlinsinmiqlng*sxqcii*a
wlmi*klxpkmklkkv*fkctkni*tqrnnqxigvsikkiyhq*qsmnliiwlkkkiks*
**stkpy*msnlllmitqvv*ltlkwvlvkmqlqcllvkfmliqmllkiyyvnfqllkll
iimiknnnnqknkql


Frame B:
---ir**tyskgfscdcyiniw*rls*lps*ipk*l*krl*ilsi*sn*mvdlcnvlfrl
gl*fenfxqk*n*krydsnapktfrrketinxlgyq*rrftindnr*i**fg*krk*kvn
dnqqnrircrifc**sprwfslh*ngyw*rcnfnvyw*slcsfkcc*kfimsifnc*nc*
*s**kttiikktnnf


Frame C:
---YDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPTKWLIXAMYYLGL
AYDLKTXSKNEIEKGMIQMHQKHLDAKKQSXYWGINKEDLPSMTIDEFNNLVEKENKKLM
IINKTVLDVESFVNDHPGGLAYIKMGIGKDATSMFTGEVYAHSNAAKNLLCQFSIAKIVD
NHDKKQQ*skkqtt

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA271 (VSA271Q) /CSM/VS/VSA2-C/VSA271Q.Seq.d/                        638   0.0
VHQ458 (VHQ458Q) /CSM/VH/VHQ4-C/VHQ458Q.Seq.d/                        638   0.0
VHQ401 (VHQ401Q) /CSM/VH/VHQ4-A/VHQ401Q.Seq.d/                        638   0.0
VHP465 (VHP465Q) /CSM/VH/VHP4-C/VHP465Q.Seq.d/                        638   0.0
VHP295 (VHP295Q) /CSM/VH/VHP2-D/VHP295Q.Seq.d/                        638   0.0
VHP289 (VHP289Q) /CSM/VH/VHP2-D/VHP289Q.Seq.d/                        638   0.0
VHN236 (VHN236Q) /CSM/VH/VHN2-B/VHN236Q.Seq.d/                        638   0.0
VHM566 (VHM566Q) /CSM/VH/VHM5-C/VHM566Q.Seq.d/                        638   0.0
VHK171 (VHK171Q) /CSM/VH/VHK1-C/VHK171Q.Seq.d/                        638   0.0
VHI146 (VHI146Q) /CSM/VH/VHI1-B/VHI146Q.Seq.d/                        638   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   638   0.0    2
AL033501|AL033501.1 C.albicans cosmid Ca41C10.                     72   4e-12  2
AL396412|AL396412.1 T3 end of clone AR0AA030B05 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    54   2e-08  2
DY893926|DY893926.1 CeleSEQ13391 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ13391, mRNA sequence.    48   2e-08  4
BM634131|BM634131.1 17000668813253 A.Gam.ad.cDNA1 Anopheles gambiae cDNA clone 19600448419669 5', mRNA sequence.    54   1e-06  2
DY885896|DY885896.1 CeleSEQ991 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ991, mRNA sequence.    48   5e-06  2
DY886077|DY886077.1 CeleSEQ1581 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ1581, mRNA sequence.    48   5e-06  2
DY895351|DY895351.1 CeleSEQ15152 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ15152, mRNA sequence.    48   5e-06  2
CR382138|CR382138.1 Debaryomyces hansenii chromosome F of strain CBS767 of Debaryomyces hansenii.    46   1e-05  2
AL392980|AL392980.1 T3 end of clone AR0AA008A11 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    44   2e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   374   e-102
AF085500_1(AF085500|pid:none) Mortierella alpina delta nine desa...   200   3e-50
Y18553_1(Y18553|pid:none) Mortierella alpina ole1 gene for stear...   200   3e-50
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   199   5e-50
AL033501_2(AL033501|pid:none) C.albicans cosmid Ca41C10.  &T1822...   197   1e-49
FM992688_770(FM992688|pid:none) Candida dubliniensis CD36 chromo...   197   2e-49
AB196774_1(AB196774|pid:none) Mortierella alpina gene for omega9...   197   2e-49
AY576801_1(AY576801|pid:none) Hortaea werneckii putative delta-9...   197   2e-49
AP007162_283(AP007162|pid:none) Aspergillus oryzae RIB40 genomic...   196   3e-49
CR382138_167(CR382138|pid:none) Debaryomyces hansenii strain CBS...   196   5e-49

 psg: 0.75  gvh: 0.34  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHA358 is nuc

>VHA358Z.Seq
NNNNNNNNNNCCATACGATGATGAACATACTCCAAAGGATTCAGTTGTGACTGCTATATT
AACATTTGGTGAAGGTTATCATAACTTCCATCATGAATTCCCAAATGACTATAGAAACGC
TTATAAATTCTATCAATATGATCCAACTAAATGGTTGATCTNTGCAATGTATTATTTAGG
CTTGGCTTATGATTTGAAAACTTTNTCCAAAAATGAAATTGAAAAAGGTATGATTCAAAT
GCACCAAAAACATTTAGACGCAAAGAAACAATCAATNTATTGGGGTATCAATAAAGAAGA
TTTACCATCAATGACAATCGATGAATTTAATAATTTGGTTGAAAAAGAAAATAAAAAGTT
AATGATAATCAACAAAACCGTATTAGATGTCGAATCTTTTGTTAATGATCACCCAGGTGG
TTTAGCTTACATTAAAATGGGTATTGGTAAAGATGCAACTTCAATGTTTACTGGTGAAGT
TTATGCTCATTCAAATGCTGCTAAAAATTTATTATGTCAATTTTCAATTGCTAAAATTGT
TGATAATCATGATAAAAAACAACAATAATCAAAAAAACAAACAACTTT