>VHA341 (VHA341Q) /CSM/VH/VHA3-B/VHA341Q.Seq.d/
GTGATATTACAAACATATAATTTTTTAAAATGCCTTTAAACCTTGTTGCTAGAAAATCAT
TAAGAGATAATGAAGAACACTTAAATAAAGCACATGAAGAAATCAAAAATGCTTTAAATG
GTGAAGAATGGATTATTGAATTTGATTGGGATGCCATTTTCGATAAAGTTGATGAACATG
TTAAAAAACAATTAGGTGAAGTATTTTATAAAAATTTATGTCCAAATATTAGTAAATGTA
TTGTAAATGCATCAAAAGATGAATTAACTAAAGAATCAATCATTAATGCAATACTGCTAA
AAAGATTGTATTAATTGTTAATGAAGATGCCAAGAATTCATCCTATTGGAAGTATGCATT
TAATGGTGGTCAATTAAATCTTTTATTCAAGAAAGGTTGTAATAATATTACTGAAGCTGG
TAATTTCGAATTATATAAAGTGATTCCAAGTGAAGGTGCTTATAGTTTACCAACTCGTCT
TAATATGAAAAAGAATCAAGAAAGATTTGAACTTGCATTCGAAAGAATTAAAGTAGTTAC
CAGTAGAGATTGGTCATTTGATGAAGCATCAATGGAAACTGTTTATCCAACTGCTTTTGA
ACATGAAAGTCAACGTGAACAATTTGGTACTACATTXXXXXXXXXXGATTTCATTAAAGT
CCTTCCAAGACCCGGGGTTTTNTCATTAGCTGCTAAAATCAATTTGAAAGAGANCCAAGA
GAAATTCGATACNGCTTTTGAAAGAATTAAAGAGGTCACTAAAGTAGATTGGTCCTATGA
TGAGCAATCATTGGAAACTGTTTATCCAGCACTTGAAGAAAGAAATAAAGAAAGAATTGG
TGACTTATTCCAAGAGATTATTAAATATGTCGCTGATAATATCGTTAAACGTTGTAAAGA
GGATATGGTTTTAGAAAGTTTCCTCGAAGCAACTTCAAATGCAAAGATTGTTTTCCGTCA
CAATGCCAAAGCAAACGGTTATTGGAATTGGAGTTTTGAAAACAATGATTTAGTAATCAC
CTTCAAATCAATTTGCAATGTTAGTGATAATGCAAACTTTGATTTCATTAAAATTTTACC
ATCACCAGGTGTACTCACTTTAGCCTCTAGAATCAATTTGAGAGAGAATCAAGAGAAAAT
CAATGAATCTTTCGAAAAGATTAAAGAAATCTTGGGTAATGATTGGACTTATGGTGAATC
TTCAATCGAACAAGTTTATCCAAAATTAGAAGAAAATAATAAACCACGTGTTGGTGATGT
CTTTGCTGAAATCATTCGTTACATCAGTCAAAATATCGTTAAAAGATGTAAAGATGATGA
TATGGTCAAAGAAGGTTTCGTTGAAGCAACTCAAAATTGTAAAATCGTCTTCCAATTTGT
TGAAAAACAAAGTTCTTATTGGGTTTGAAAATTCGATGGTGGTAATTTAGTCGTATCATT
CAAATCAATTTGTAATACTAGTGATAATGCAAATTTCAATTTTGAAGCTCTCCTTTAAAA
TTAAGAAAAAAATAAAAATAAAAATAAAAAAAGNATAATAGAAAATAGTATTNCTCNCTT
TT

*yykhiif*nafkpcc*kiikr**rtlk*st*rnqkcfkw*rmdy*i*lgchfr*s**tc
*ktir*sil*kfmsky**myckcikr*in*rinh*CNTAKKIVLIVNEDAKNSSYWKYAF
NGGQLNLLFKKGCNNITEAGNFELYKVIPSEGAYSLPTRLNMKKNQERFELAFERIKVVT
SRDWSFDEASMETVYPTAFEHESQREQFGTT---

---DFIKVLPRPGVXSLAAKINLKEXQEKFDTAFERIKEVTKVDWSYDEQSLETVYPALE
ERNKERIGDLFQEIIKYVADNIVKRCKEDMVLESFLEATSNAKIVFRHNAKANGYWNWSF
ENNDLVITFKSICNVSDNANFDFIKILPSPGVLTLASRINLRENQEKINESFEKIKEILG
NDWTYGESSIEQVYPKLEENNKPRVGDVFAEIIRYISQNIVKRCKDDDMVKEGFVEATQN
CKIVFQFVEKQSSYWV*kfdggnlvvsfksicntsdnanfnfeall*n*eknknknkkxi
iensixxf

Frame A:
vilqtynflkcl*tlllenh*eimknt*ikhmkkskml*mvkngllnligmpfsiklmnm
lknn*vkyfikiyvqilvnvl*mhqkmn*lknqslmqyc*kdcinc**rcqefillevci
*wwsiksfiqerl**yy*sw*frii*sdsk*rcl*ftnss*yekesrki*tcirkn*ssy
q*rlvi**singnclsncf*t*kst*tiwyyi---

---DFIKVLPRPGVXSLAAKINLKEXQEKFDTAFERIKEVTKVDWSYDEQSLETVYPALE
ERNKERIGDLFQEIIKYVADNIVKRCKEDMVLESFLEATSNAKIVFRHNAKANGYWNWSF
ENNDLVITFKSICNVSDNANFDFIKILPSPGVLTLASRINLRENQEKINESFEKIKEILG
NDWTYGESSIEQVYPKLEENNKPRVGDVFAEIIRYISQNIVKRCKDDDMVKEGFVEATQN
CKIVFQFVEKQSSYWV*kfdggnlvvsfksicntsdnanfnfeall*n*eknknknkkxi
iensixxf

Frame B:
*yykhiif*nafkpcc*kiikr**rtlk*st*rnqkcfkw*rmdy*i*lgchfr*s**tc
*ktir*sil*kfmsky**myckcikr*in*rinh*CNTAKKIVLIVNEDAKNSSYWKYAF
NGGQLNLLFKKGCNNITEAGNFELYKVIPSEGAYSLPTRLNMKKNQERFELAFERIKVVT
SRDWSFDEASMETVYPTAFEHESQREQFGTT---

---islksfqdpgfxh*llksi*krxkrnsixllkelkrslk*igpmmsnhwklfiqhlk
keikkelvtyskrllnmsliislnvvkriwf*kvsskqlqmqrlfsvtmpkqtvigigvl
ktmi**spsnqfamlvimqtlislkfyhhqvysl*plesi*erikrksmnlskrlkkswv
miglmvnlqsnkfiqn*kkiinhvlvmsllksfvtsvkislkdvkmmiwskkvslkqlki
vksssnllknkvligfensmvvi*syhsnqfvilvimqisilklsfkikkkikikikkx*
*kivxlxf

Frame C:
ditni*ffkmplnlvarkslrdneehlnkaheeiknalngeewiiefdwdaifdkvdehv
kkqlgevfyknlcpniskcivnaskdeltkesiinaillkrly*llmkmprihpigsmhl
mvvn*ifysrkvviillklvisnyik*fqvkvlivyqlvli*krikkdlnlhskelk*lp
veighlmkhqwklfiqlllnmkvnvnnlvlh---

---fh*spsktrgfxisc*nqferxpreiryxf*kn*rgh*srlvl**aiignclsst*r
kk*rknw*liprdy*icr**yr*tl*rgygfrkfprsnfkckdcfpsqcqskrllelef*
kq*fsnhlqinlqc***ckl*fh*nftitrcthfsl*nqferesrenq*ifrkd*rnlg*
*ldlw*ifnrtslskirrk**ttcw*clc*nhslhqskyr*km*r**ygqrrfr*snskl
*nrlpic*ktkfllglkirww*fsriiqinl*y***ckfqf*ssplklrkk*k*k*kkxn
rk*yxsl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA341 (VHA341Q) /CSM/VH/VHA3-B/VHA341Q.Seq.d/                       2906   0.0
VHA337 (VHA337Q) /CSM/VH/VHA3-B/VHA337Q.Seq.d/                       1622   0.0
VHO859 (VHO859Q) /CSM/VH/VHO8-C/VHO859Q.Seq.d/                       1620   0.0
VHK896 (VHK896Q) /CSM/VH/VHK8-D/VHK896Q.Seq.d/                       1614   0.0
VHJ523 (VHJ523Q) /CSM/VH/VHJ5-A/VHJ523Q.Seq.d/                       1612   0.0
VHD261 (VHD261Q) /CSM/VH/VHD2-C/VHD261Q.Seq.d/                       1610   0.0
VHO667 (VHO667Q) /CSM/VH/VHO6-C/VHO667Q.Seq.d/                       1606   0.0
VHJ560 (VHJ560Q) /CSM/VH/VHJ5-C/VHJ560Q.Seq.d/                       1604   0.0
VHH740 (VHH740Q) /CSM/VH/VHH7-B/VHH740Q.Seq.d/                       1602   0.0
VHC827 (VHC827Q) /CSM/VH/VHC8-B/VHC827Q.Seq.d/                       1596   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117081|AC117081.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    56   4e-10  9
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          36   0.010  22
AE014817|AE014817.1 Plasmodium falciparum 3D7 chromosome 14 section 2 of 13 of the complete sequence.    36   0.020  11
AC098590|AC098590.3 Homo sapiens BAC clone RP11-324J13 from 4, complete sequence.    42   0.045  6
Z54142|Z54142.2 S.pombe chromosome I cosmid c24H6.                 38   0.12   5
AC133975|AC133975.2 Rattus norvegicus clone CH230-10P19, WORKING DRAFT SEQUENCE.    50   0.19   1
AC122629|AC122629.4 Rattus norvegicus clone CH230-102A4, WORKING DRAFT SEQUENCE, 2 unordered pieces.    50   0.19   1
AC121167|AC121167.4 Rattus norvegicus clone CH230-108M4, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    50   0.19   1
AC114143|AC114143.4 Rattus norvegicus clone CH230-141A22, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    50   0.19   1
CT458730|CT458730.1 Sus scrofa genomic clone CH242-523I4, genomic survey sequence.    50   0.19   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117081_29(AC117081|pid:none) Dictyostelium discoideum chromoso...   154   1e-35
AC117081_21(AC117081|pid:none) Dictyostelium discoideum chromoso...   153   1e-35
AJ344328_29(AJ344328|pid:none) Laminaria digitata complete mitoc...    40   0.23
(P43040) RecName: Full=Uncharacterized protein MCAP_0005; AltNam...    39   0.68
AL844502_159(AL844502|pid:none) Plasmodium falciparum 3D7 chromo...    38   1.2
(P47166) RecName: Full=Protein SGM1; AltName: Full=Slow growth o...    37   2.0
AE017245_484(AE017245|pid:none) Mycoplasma synoviae 53, complete...    37   2.6
AY587609_1(AY587609|pid:none) Ascaridia galli lipid binding prot...    37   2.6
AM180355_1608(AM180355|pid:none) Clostridium difficile 630 compl...    37   2.6
CP000382_1316(CP000382|pid:none) Clostridium novyi NT, complete ...    36   3.4

 psg: 0.67  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VHA341 is cyt

>VHA341F.Seq
GTGATATTACAAACATATAATTTTTTAAAATGCCTTTAAACCTTGTTGCTAGAAAATCAT
TAAGAGATAATGAAGAACACTTAAATAAAGCACATGAAGAAATCAAAAATGCTTTAAATG
GTGAAGAATGGATTATTGAATTTGATTGGGATGCCATTTTCGATAAAGTTGATGAACATG
TTAAAAAACAATTAGGTGAAGTATTTTATAAAAATTTATGTCCAAATATTAGTAAATGTA
TTGTAAATGCATCAAAAGATGAATTAACTAAAGAATCAATCATTAATGCAATACTGCTAA
AAAGATTGTATTAATTGTTAATGAAGATGCCAAGAATTCATCCTATTGGAAGTATGCATT
TAATGGTGGTCAATTAAATCTTTTATTCAAGAAAGGTTGTAATAATATTACTGAAGCTGG
TAATTTCGAATTATATAAAGTGATTCCAAGTGAAGGTGCTTATAGTTTACCAACTCGTCT
TAATATGAAAAAGAATCAAGAAAGATTTGAACTTGCATTCGAAAGAATTAAAGTAGTTAC
CAGTAGAGATTGGTCATTTGATGAAGCATCAATGGAAACTGTTTATCCAACTGCTTTTGA
ACATGAAAGTCAACGTGAACAATTTGGTACTACATTNNNNNNNNNN

>VHA341Z.Seq
NNNNNNNNNNGATTTCATTAAAGTCCTTCCAAGACCCGGGGTTTTNTCATTAGCTGCTAA
AATCAATTTGAAAGAGANCCAAGAGAAATTCGATACNGCTTTTGAAAGAATTAAAGAGGT
CACTAAAGTAGATTGGTCCTATGATGAGCAATCATTGGAAACTGTTTATCCAGCACTTGA
AGAAAGAAATAAAGAAAGAATTGGTGACTTATTCCAAGAGATTATTAAATATGTCGCTGA
TAATATCGTTAAACGTTGTAAAGAGGATATGGTTTTAGAAAGTTTCCTCGAAGCAACTTC
AAATGCAAAGATTGTTTTCCGTCACAATGCCAAAGCAAACGGTTATTGGAATTGGAGTTT
TGAAAACAATGATTTAGTAATCACCTTCAAATCAATTTGCAATGTTAGTGATAATGCAAA
CTTTGATTTCATTAAAATTTTACCATCACCAGGTGTACTCACTTTAGCCTCTAGAATCAA
TTTGAGAGAGAATCAAGAGAAAATCAATGAATCTTTCGAAAAGATTAAAGAAATCTTGGG
TAATGATTGGACTTATGGTGAATCTTCAATCGAACAAGTTTATCCAAAATTAGAAGAAAA
TAATAAACCACGTGTTGGTGATGTCTTTGCTGAAATCATTCGTTACATCAGTCAAAATAT
CGTTAAAAGATGTAAAGATGATGATATGGTCAAAGAAGGTTTCGTTGAAGCAACTCAAAA
TTGTAAAATCGTCTTCCAATTTGTTGAAAAACAAAGTTCTTATTGGGTTTGAAAATTCGA
TGGTGGTAATTTAGTCGTATCATTCAAATCAATTTGTAATACTAGTGATAATGCAAATTT
CAATTTTGAAGCTCTCCTTTAAAATTAAGAAAAAAATAAAAATAAAAATAAAAAAAGNAT
AATAGAAAATAGTATTNCTCNCTTTT

>VHA341P.Seq
GTGATATTACAAACATATAATTTTTTAAAATGCCTTTAAACCTTGTTGCTAGAAAATCAT
TAAGAGATAATGAAGAACACTTAAATAAAGCACATGAAGAAATCAAAAATGCTTTAAATG
GTGAAGAATGGATTATTGAATTTGATTGGGATGCCATTTTCGATAAAGTTGATGAACATG
TTAAAAAACAATTAGGTGAAGTATTTTATAAAAATTTATGTCCAAATATTAGTAAATGTA
TTGTAAATGCATCAAAAGATGAATTAACTAAAGAATCAATCATTAATGCAATACTGCTAA
AAAGATTGTATTAATTGTTAATGAAGATGCCAAGAATTCATCCTATTGGAAGTATGCATT
TAATGGTGGTCAATTAAATCTTTTATTCAAGAAAGGTTGTAATAATATTACTGAAGCTGG
TAATTTCGAATTATATAAAGTGATTCCAAGTGAAGGTGCTTATAGTTTACCAACTCGTCT
TAATATGAAAAAGAATCAAGAAAGATTTGAACTTGCATTCGAAAGAATTAAAGTAGTTAC
CAGTAGAGATTGGTCATTTGATGAAGCATCAATGGAAACTGTTTATCCAACTGCTTTTGA
ACATGAAAGTCAACGTGAACAATTTGGTACTACATT----------GATTTCATTAAAGT
CCTTCCAAGACCCGGGGTTTTNTCATTAGCTGCTAAAATCAATTTGAAAGAGANCCAAGA
GAAATTCGATACNGCTTTTGAAAGAATTAAAGAGGTCACTAAAGTAGATTGGTCCTATGA
TGAGCAATCATTGGAAACTGTTTATCCAGCACTTGAAGAAAGAAATAAAGAAAGAATTGG
TGACTTATTCCAAGAGATTATTAAATATGTCGCTGATAATATCGTTAAACGTTGTAAAGA
GGATATGGTTTTAGAAAGTTTCCTCGAAGCAACTTCAAATGCAAAGATTGTTTTCCGTCA
CAATGCCAAAGCAAACGGTTATTGGAATTGGAGTTTTGAAAACAATGATTTAGTAATCAC
CTTCAAATCAATTTGCAATGTTAGTGATAATGCAAACTTTGATTTCATTAAAATTTTACC
ATCACCAGGTGTACTCACTTTAGCCTCTAGAATCAATTTGAGAGAGAATCAAGAGAAAAT
CAATGAATCTTTCGAAAAGATTAAAGAAATCTTGGGTAATGATTGGACTTATGGTGAATC
TTCAATCGAACAAGTTTATCCAAAATTAGAAGAAAATAATAAACCACGTGTTGGTGATGT
CTTTGCTGAAATCATTCGTTACATCAGTCAAAATATCGTTAAAAGATGTAAAGATGATGA
TATGGTCAAAGAAGGTTTCGTTGAAGCAACTCAAAATTGTAAAATCGTCTTCCAATTTGT
TGAAAAACAAAGTTCTTATTGGGTTTGAAAATTCGATGGTGGTAATTTAGTCGTATCATT
CAAATCAATTTGTAATACTAGTGATAATGCAAATTTCAATTTTGAAGCTCTCCTTTAAAA
TTAAGAAAAAAATAAAAATAAAAATAAAAAAAGNATAATAGAAAATAGTATTNCTCNCTT
TT