>VHA326 (VHA326Q) /CSM/VH/VHA3-B/VHA326Q.Seq.d/
XXXXXXXXXXAAAAGAGAATACCCAACTATCGTCCATCATGGAATTTAACCGTGGTTCAA
CCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAA
TCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGT
CAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAA
CCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCA
AGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCA
ATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTAC
TCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGG
TTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGT
CAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATT
ACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCA
AGATTAAAATGGAAAACTGTT

---KRIPNYRPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQ
LDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPM
FTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQ
DSQDPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTV

Frame A:
---kentqlssimefnrgstnqw*lctcecyclhsrs*siitihhch**kswmcivkrws
igyddassyfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpn
vynhstifkrlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*ws
rftrstnyhfrsfnylfnnynhfcn*nefnwcskiikfikikmenc


Frame B:
---KRIPNYRPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQ
LDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPM
FTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQ
DSQDPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTV


Frame C:
---reyptivhhgi*pwfnqpvvimyl*mllptfkilinhynsplslievvdvhr*emvn
wi**civvl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqc
lqplnnlqtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvk
ihkihklsl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngkl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA326 (VHA326Q) /CSM/VH/VHA3-B/VHA326Q.Seq.d/                       1330   0.0
VHM887 (VHM887Q) /CSM/VH/VHM8-D/VHM887Q.Seq.d/                       1324   0.0
VHM790 (VHM790Q) /CSM/VH/VHM7-D/VHM790Q.Seq.d/                       1324   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1324   0.0
SHB338 (SHB338Q) /CSM/SH/SHB3-B/SHB338Q.Seq.d/                       1324   0.0
AHC569 (AHC569Q) /CSM/AH/AHC5-C/AHC569Q.Seq.d/                       1324   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1322   0.0
VHP677 (VHP677Q) /CSM/VH/VHP6-D/VHP677Q.Seq.d/                       1322   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1322   0.0
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                       1322   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1298   0.0    1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   0.100  8
AP007341|AP007341.1 Lotus japonicus genomic DNA, chromosome 2, clone:LjT35G04, TM0425.    40   0.11   2
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   1.3    1
AM025232|AM025232.1 Bos indicus 3' EST, clone C0005915l21.         34   3.2    2
BH703301|BH703301.1 BOMNG55TR BO_2_3_KB Brassica oleracea genomic clone BOMNG55, DNA sequence.    40   3.5    2
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   4.2    2
EH243429|EH243429.1 JGI_ACAC13551.fwd ACAC Phakopsora pachyrhizi TW72-1 germinating urediniospores on water surface (20C/16 hours/dark) Phakopsora pachyrhizi cDNA clone ACAC13551 5', mRNA sequence.    44   5.0    1
AV262947|AV262947.1 Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930432P20, 3' end partial sequence.    44   5.0    1
EH220383|EH220383.1 JGI_ABWZ1277.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA clone ABWZ1277 3', mRNA sequence.    44   5.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   378   e-104
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   378   e-104
BT036325_1(BT036325|pid:none) Zea mays full-length cDNA clone ZM...   105   1e-21
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   104   2e-21
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   103   6e-21
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   100   5e-20
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   100   5e-20
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...    99   1e-19
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...    99   2e-19
EF146940_1(EF146940|pid:none) Populus trichocarpa clone WS01225_...    98   2e-19

 psg: 0.82  gvh: 0.51  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.43  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: mitochondrial
	 28.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VHA326 is mit

>VHA326Z.Seq
NNNNNNNNNNAAAAGAGAATACCCAACTATCGTCCATCATGGAATTTAACCGTGGTTCAA
CCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAA
TCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGT
CAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAA
CCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCA
AGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCA
ATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTAC
TCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGG
TTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGT
CAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATT
ACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCA
AGATTAAAATGGAAAACTGTT