>VHA296 (VHA296Q) /CSM/VH/VHA2-D/VHA296Q.Seq.d/
GTATCTCTATAATAGACAAGTAATAAATGGTTAACTTAAATCCACAAACAATNGCAGAGA
TCGAAGAATGTCTTCGTGCATTACTCCAACCAAACAATGATATTATTAAAGCTGCAACTG
ATAAATTAAATAAATTATTAAAAAAGAGTGAATATTCATTATATCTTTTCCATTTATTAG
AAAGTTCACCATATGATGAGATTAAACAATTATCAGCAGTATTATTAAGACAAAAATTAG
TTGCACATTGGACTAAATTTTCAGTTGAAAGTAGAAAATATATTAAAGATTCAATCCTTA
AATTAGTTATCTCTCAACCAAGTCAATTAGTTAGACGTTCGATCTCTGAGGTTATTATTA
TCATTGCTAGATTAGAAGTTGCAACTGGTACTTGGGGCGATTTATTCCCATTCCTTCTTC
AATCATCTTCAAGTCCTGATACTATCGTTAGACAAATTCAAATTCATATTTTAGATTCAT
TAATTTCAAATGTCGATGTTTTCTTAAAATATTTCCCACAACTTCCAACTGTACTCTCAC
AAGCTGTTATTGATCCACAATTATCAGTTAGAGCATTGGCAGTAAAAGCAATTGGTTCTT
CAATTTATGCTGTTCAAACTGATTCANAACTXXXXXXXXXXTTAGATGCTTCATTACCAT
TCCTCTTTAAATTATCACGTTCCTCAACAATCCATCATTCAATTAAAGCTGTTGTAATTG
GTACAATCGCAGAATGTTTCAAATTGATTGGATCCGGACTACAGTGCTTATTTACCAAGA
ATACTTANATTCACTTGGAAAGCATTAAAGAATCAAGAAGAGTNTGCAAAAGTTCAAAGA
GTTNGTTGTTTCCTCTTGGGTGTAACATTACAAAATTCAATTAAAGCCACTCAAGATCAA
TATCTTCAAACTTTACAATTGAGTTCACCAATCATTATGAAACAAGATCAAGATCCATTG
GTATTAGATAATGCAATTGGTTGTATTTGTCGTATGATCGNTGGTAATCAATCATTTGTA
CCAGTTGNTACAGCTTTACCAGTTTTAATCTCAAAATTNCCAATTCAAAAAGATCATGAA
GAAATTGATCCAGTTTTAAATGCTCTCTTGGTATTATTCAATANTCAATTCGATTTAATC
GCTCCACATACTCAAACTATTGTTCAATCTTTAGCTCCATACATGAATANATCAAAATAT
CCTTTAAAACAAACGACTTNTGATANAATTCAACAAATCATTCATAGTTTAATTGTTAAA
TTCCCAGATCAAATGAAACAAATTTTATCAAATATTCCAGAATTTCAGAAATATTAAATT
TTAATTTTNTAAAATAATAAAAATAAAAATTAAAAGTTNT

isiidk**MVNLNPQTXAEIEECLRALLQPNNDIIKAATDKLNKLLKKSEYSLYLFHLLE
SSPYDEIKQLSAVLLRQKLVAHWTKFSVESRKYIKDSILKLVISQPSQLVRRSISEVIII
IARLEVATGTWGDLFPFLLQSSSSPDTIVRQIQIHILDSLISNVDVFLKYFPQLPTVLSQ
AVIDPQLSVRALAVKAIGSSIYAVQTDSX---

---LDASLPFLFKLSRSSTIHHSIKAVVIGTIAECFKLIGSGLQCLFTKNTXIHLESIKE
SRRVCKSSKSXLFPLGCNITKFN*shsrsissnftieftnhyetrsrsigir*cnwlyls
ydrw*siictsxysftsfnlkixnskrs*rn*ssfkcslgiiqxsirfnrstysnycsif
ssihexikisfktndx*xnstnhs*fnc*iprsnetnfikysriseilnfnfxk**k*kl
kv

Frame A:
vsl**tsnkwlt*ihkqxqrsknvfvhysnqtmillklqlin*iny*krvnihyifsiy*
kvhhmmrlnnyqqyy*dkn*lhiglnfqlkvenilkiqsln*lslnqvn*ldvrslrlll
slld*klqlvlgaiyshsffnhlqvlilsldkfkfif*ih*fqmsmfs*nishnfqlysh
klllihnyqlehwq*kqlvlqfmlfklixn---

---LDASLPFLFKLSRSSTIHHSIKAVVIGTIAECFKLIGSGLQCLFTKNTXIHLESIKE
SRRVCKSSKSXLFPLGCNITKFN*shsrsissnftieftnhyetrsrsigir*cnwlyls
ydrw*siictsxysftsfnlkixnskrs*rn*ssfkcslgiiqxsirfnrstysnycsif
ssihexikisfktndx*xnstnhs*fnc*iprsnetnfikysriseilnfnfxk**k*kl
kv

Frame B:
ylynrqving*lkstnnxrdrrmsscitptkq*yy*scn**ik*iikke*ifiisfpfir
kfti**d*tiissiiktkisctld*ifs*k*kiy*rfnp*isylstksis*tfdl*gyyy
hc*irscnwylgrfipipssiifks*yyr*tnsnsyfrfinfkcrcflkifpttsnctlt
scy*stiis*sigsksnwffnlccsn*fxt---

---*mlhyhsslnyhvpqqsiiqlkll*lvqsqnvsn*ldpdysaylprilxftwkalkn
qeexakvqrvxcfllgvtlqnsikatqdqylqtlqlsspiimkqdqdplvldnaigcicr
mixgnqsfvpvxtalpvliskxpiqkdheeidpvlnallvlfnxqfdliaphtqtivqsl
apymnxskyplkqttxdxiqqiihslivkfpdqmkqilsnipefqky*ilix*nnknkn*
kx

Frame C:
isiidk**MVNLNPQTXAEIEECLRALLQPNNDIIKAATDKLNKLLKKSEYSLYLFHLLE
SSPYDEIKQLSAVLLRQKLVAHWTKFSVESRKYIKDSILKLVISQPSQLVRRSISEVIII
IARLEVATGTWGDLFPFLLQSSSSPDTIVRQIQIHILDSLISNVDVFLKYFPQLPTVLSQ
AVIDPQLSVRALAVKAIGSSIYAVQTDSX---

---rcfitipl*iitflnnpsfn*sccnwynrrmfqidwirttvliyqeylxslgkh*ri
kksxqkfkexvvsswv*hykiqlkplkinifklyn*vhqsl*nkikihwy*imqlvvfvv
*sxvinhlyqlxqlyqf*sqnxqfkkimkkliqf*mlswyysixnsi*slhilkllfnl*
lht*ixqnil*nkrlxixfnksfiv*llnsqik*nkfyqifqnfrnikf*fxkiikikik
sx

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA296 (VHA296Q) /CSM/VH/VHA2-D/VHA296Q.Seq.d/                       2407   0.0
VHL596 (VHL596Q) /CSM/VH/VHL5-D/VHL596Q.Seq.d/                       1227   0.0
VHH290 (VHH290Q) /CSM/VH/VHH2-D/VHH290Q.Seq.d/                       1193   0.0
VHI716 (VHI716Q) /CSM/VH/VHI7-A/VHI716Q.Seq.d/                       1170   0.0
VHD333 (VHD333Q) /CSM/VH/VHD3-B/VHD333Q.Seq.d/                       1168   0.0
VHL167 (VHL167Q) /CSM/VH/VHL1-C/VHL167Q.Seq.d/                       1154   0.0
VSB470 (VSB470Q) /CSM/VS/VSB4-C/VSB470Q.Seq.d/                         44   0.006
VHE725 (VHE725Q) /CSM/VH/VHE7-B/VHE725Q.Seq.d/                         44   0.006
SLK512 (SLK512Q) /CSM/SL/SLK5-A/SLK512Q.Seq.d/                         44   0.006
SHF650 (SHF650Q) /CSM/SH/SHF6-C/SHF650Q.Seq.d/                         44   0.006

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BV679697|BV679697.1 TMB1897 Upland cotton TM-1 Gossypium hirsutum STS genomic clone C47-F12-SP10_A04_02.ab1, sequence tagged site.    48   0.17   2
AC118708|AC118708.4 Mus musculus chromosome 7, clone RP24-118K11, complete sequence.    38   0.20   7
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   0.41   11
AE014817|AE014817.1 Plasmodium falciparum 3D7 chromosome 14 section 2 of 13 of the complete sequence.    38   0.61   9
EK387273|EK387273.1 1095469488219 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005840883 3', genomic survey sequence.    32   0.64   4
AC189191|AC189191.1 Brassica rapa subsp. pekinensis clone KBrB002K01, *** SEQUENCING IN PROGRESS ***, 4 ordered pieces.    48   0.64   1
EI344508|EI344508.1 GM_WBc0089K23.r GM_WBc Glycine max genomic clone GM_WBc0089K23 3', genomic survey sequence.    48   0.64   1
ED522671|ED522671.1 KBrB110J15F KBrB, Brassica rapa BamHI BAC library Brassica rapa subsp. pekinensis genomic clone KBrB110J15, genomic survey sequence.    48   0.64   1
DX039929|DX039929.1 KBrB039I13R KBrB, Brassica rapa BamHI BAC library Brassica rapa subsp. pekinensis genomic clone KBrB039I13, genomic survey sequence.    48   0.64   1
CT963076|CT963076.3 M.truncatula DNA sequence from clone MTH2-22P7 on chromosome 3.    48   0.64   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM437344_1(AM437344|pid:none) Vitis vinifera contig VV78X175554....    80   2e-13
AC135206_1(AC135206|pid:none) Oryza sativa (japonica cultivar-gr...    76   3e-12
AL035602_13(AL035602|pid:none) Arabidopsis thaliana DNA chromoso...    75   5e-12
CR380953_142(CR380953|pid:none) Candida glabrata strain CBS138 c...    73   3e-11
AE016820_73(AE016820|pid:none) Ashbya gossypii (= Eremothecium g...    67   1e-09
CU928167_261(CU928167|pid:none) Kluyveromyces thermotolerans str...    64   2e-08
BC158356_1(BC158356|pid:none) Xenopus tropicalis hypothetical pr...    63   2e-08
(P40069) RecName: Full=Importin subunit beta-4; AltName: Full=Ka...    62   4e-08
AK100569_1(AK100569|pid:none) Oryza sativa Japonica Group cDNA c...    57   1e-06
AP008209_981(AP008209|pid:none) Oryza sativa (japonica cultivar-...    57   1e-06

 psg: 0.75  gvh: 0.49  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: peroxisomal
	  4.0 %: mitochondrial

>> prediction for VHA296 is cyt

>VHA296F.Seq
GTATCTCTATAATAGACAAGTAATAAATGGTTAACTTAAATCCACAAACAATNGCAGAGA
TCGAAGAATGTCTTCGTGCATTACTCCAACCAAACAATGATATTATTAAAGCTGCAACTG
ATAAATTAAATAAATTATTAAAAAAGAGTGAATATTCATTATATCTTTTCCATTTATTAG
AAAGTTCACCATATGATGAGATTAAACAATTATCAGCAGTATTATTAAGACAAAAATTAG
TTGCACATTGGACTAAATTTTCAGTTGAAAGTAGAAAATATATTAAAGATTCAATCCTTA
AATTAGTTATCTCTCAACCAAGTCAATTAGTTAGACGTTCGATCTCTGAGGTTATTATTA
TCATTGCTAGATTAGAAGTTGCAACTGGTACTTGGGGCGATTTATTCCCATTCCTTCTTC
AATCATCTTCAAGTCCTGATACTATCGTTAGACAAATTCAAATTCATATTTTAGATTCAT
TAATTTCAAATGTCGATGTTTTCTTAAAATATTTCCCACAACTTCCAACTGTACTCTCAC
AAGCTGTTATTGATCCACAATTATCAGTTAGAGCATTGGCAGTAAAAGCAATTGGTTCTT
CAATTTATGCTGTTCAAACTGATTCANAACTNNNNNNNNNN

>VHA296Z.Seq
NNNNNNNNNNTTAGATGCTTCATTACCATTCCTCTTTAAATTATCACGTTCCTCAACAAT
CCATCATTCAATTAAAGCTGTTGTAATTGGTACAATCGCAGAATGTTTCAAATTGATTGG
ATCCGGACTACAGTGCTTATTTACCAAGAATACTTANATTCACTTGGAAAGCATTAAAGA
ATCAAGAAGAGTNTGCAAAAGTTCAAAGAGTTNGTTGTTTCCTCTTGGGTGTAACATTAC
AAAATTCAATTAAAGCCACTCAAGATCAATATCTTCAAACTTTACAATTGAGTTCACCAA
TCATTATGAAACAAGATCAAGATCCATTGGTATTAGATAATGCAATTGGTTGTATTTGTC
GTATGATCGNTGGTAATCAATCATTTGTACCAGTTGNTACAGCTTTACCAGTTTTAATCT
CAAAATTNCCAATTCAAAAAGATCATGAAGAAATTGATCCAGTTTTAAATGCTCTCTTGG
TATTATTCAATANTCAATTCGATTTAATCGCTCCACATACTCAAACTATTGTTCAATCTT
TAGCTCCATACATGAATANATCAAAATATCCTTTAAAACAAACGACTTNTGATANAATTC
AACAAATCATTCATAGTTTAATTGTTAAATTCCCAGATCAAATGAAACAAATTTTATCAA
ATATTCCAGAATTTCAGAAATATTAAATTTTAATTTTNTAAAATAATAAAAATAAAAATT
AAAAGTTNT

>VHA296P.Seq
GTATCTCTATAATAGACAAGTAATAAATGGTTAACTTAAATCCACAAACAATNGCAGAGA
TCGAAGAATGTCTTCGTGCATTACTCCAACCAAACAATGATATTATTAAAGCTGCAACTG
ATAAATTAAATAAATTATTAAAAAAGAGTGAATATTCATTATATCTTTTCCATTTATTAG
AAAGTTCACCATATGATGAGATTAAACAATTATCAGCAGTATTATTAAGACAAAAATTAG
TTGCACATTGGACTAAATTTTCAGTTGAAAGTAGAAAATATATTAAAGATTCAATCCTTA
AATTAGTTATCTCTCAACCAAGTCAATTAGTTAGACGTTCGATCTCTGAGGTTATTATTA
TCATTGCTAGATTAGAAGTTGCAACTGGTACTTGGGGCGATTTATTCCCATTCCTTCTTC
AATCATCTTCAAGTCCTGATACTATCGTTAGACAAATTCAAATTCATATTTTAGATTCAT
TAATTTCAAATGTCGATGTTTTCTTAAAATATTTCCCACAACTTCCAACTGTACTCTCAC
AAGCTGTTATTGATCCACAATTATCAGTTAGAGCATTGGCAGTAAAAGCAATTGGTTCTT
CAATTTATGCTGTTCAAACTGATTCANAACT----------TTAGATGCTTCATTACCAT
TCCTCTTTAAATTATCACGTTCCTCAACAATCCATCATTCAATTAAAGCTGTTGTAATTG
GTACAATCGCAGAATGTTTCAAATTGATTGGATCCGGACTACAGTGCTTATTTACCAAGA
ATACTTANATTCACTTGGAAAGCATTAAAGAATCAAGAAGAGTNTGCAAAAGTTCAAAGA
GTTNGTTGTTTCCTCTTGGGTGTAACATTACAAAATTCAATTAAAGCCACTCAAGATCAA
TATCTTCAAACTTTACAATTGAGTTCACCAATCATTATGAAACAAGATCAAGATCCATTG
GTATTAGATAATGCAATTGGTTGTATTTGTCGTATGATCGNTGGTAATCAATCATTTGTA
CCAGTTGNTACAGCTTTACCAGTTTTAATCTCAAAATTNCCAATTCAAAAAGATCATGAA
GAAATTGATCCAGTTTTAAATGCTCTCTTGGTATTATTCAATANTCAATTCGATTTAATC
GCTCCACATACTCAAACTATTGTTCAATCTTTAGCTCCATACATGAATANATCAAAATAT
CCTTTAAAACAAACGACTTNTGATANAATTCAACAAATCATTCATAGTTTAATTGTTAAA
TTCCCAGATCAAATGAAACAAATTTTATCAAATATTCCAGAATTTCAGAAATATTAAATT
TTAATTTTNTAAAATAATAAAAATAAAAATTAAAAGTTNT