>VHA295 (VHA295Q) /CSM/VH/VHA2-D/VHA295Q.Seq.d/
XXXXXXXXXXAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAA
GATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCG
TTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGT
GGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTC
CATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACAT
CCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATAT
CAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAA
ACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTAT
CAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATC
TTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTA
TCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAATAACTGCTCCTCAGT

---PTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRG
VGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQ
GVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIF
STITITSATEMNLTGVQKLSNLSRLKWKTVDGK*llls

Frame A:
---tnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddassyfer*wsw
cwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefsis
rclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfnyl
fnnynhfcn*nefnwcskiikfikikmenc*w*itapq


Frame B:
---PTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRG
VGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQ
GVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIF
STITITSATEMNLTGVQKLSNLSRLKWKTVDGK*llls


Frame C:
---qpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi**civvl*kmmvvv
lvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigilnik
vfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifqlsf
qqlqsllqlk*i*lvfknyqiyqd*ngkllmvnncss

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA295 (VHA295Q) /CSM/VH/VHA2-D/VHA295Q.Seq.d/                       1283   0.0
VHB657 (VHB657Q) /CSM/VH/VHB6-C/VHB657Q.Seq.d/                       1277   0.0
AHO594 (AHO594Q) /CSM/AH/AHO5-D/AHO594Q.Seq.d/                       1265   0.0
AHL213 (AHL213Q) /CSM/AH/AHL2-A/AHL213Q.Seq.d/                       1263   0.0
VHO223 (VHO223Q) /CSM/VH/VHO2-A/VHO223Q.Seq.d/                       1261   0.0
SHL810 (SHL810Q) /CSM/SH/SHL8-A/SHL810Q.Seq.d/                       1259   0.0
SHL768 (SHL768Q) /CSM/SH/SHL7-C/SHL768Q.Seq.d/                       1259   0.0
CHL231 (CHL231Q) /CSM/CH/CHL2-B/CHL231Q.Seq.d/                       1259   0.0
AHK685 (AHK685Q) /CSM/AH/AHK6-D/AHK685Q.Seq.d/                       1259   0.0
AHF895 (AHF895Q) /CSM/AH/AHF8-D/AHF895Q.Seq.d/                       1259   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1229   0.0    1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   0.079  8
AP007341|AP007341.1 Lotus japonicus genomic DNA, chromosome 2, clone:LjT35G04, TM0425.    40   0.10   2
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   1.2    1
CT485998|CT485998.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-104K5.    32   2.7    6
AM025232|AM025232.1 Bos indicus 3' EST, clone C0005915l21.         34   3.1    2
BH703301|BH703301.1 BOMNG55TR BO_2_3_KB Brassica oleracea genomic clone BOMNG55, DNA sequence.    40   3.3    2
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   3.9    2
EH243429|EH243429.1 JGI_ACAC13551.fwd ACAC Phakopsora pachyrhizi TW72-1 germinating urediniospores on water surface (20C/16 hours/dark) Phakopsora pachyrhizi cDNA clone ACAC13551 5', mRNA sequence.    44   4.8    1
AV262947|AV262947.1 Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930432P20, 3' end partial sequence.    44   4.8    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   353   2e-96
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   353   2e-96
BT036325_1(BT036325|pid:none) Zea mays full-length cDNA clone ZM...    91   3e-17
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...    89   1e-16
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...    88   3e-16
EF146940_1(EF146940|pid:none) Populus trichocarpa clone WS01225_...    85   2e-15
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...    84   3e-15
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...    84   5e-15
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...    84   5e-15
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...    82   1e-14

 psg: 0.82  gvh: 0.45  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHA295 is cyt

>VHA295Z.Seq
NNNNNNNNNNAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAA
GATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCG
TTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGT
GGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTC
CATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACAT
CCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATAT
CAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAA
ACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTAT
CAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATC
TTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTA
TCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAATAACTGCTCCTCAGT