>VHA293 (VHA293Q) /CSM/VH/VHA2-D/VHA293Q.Seq.d/
TATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCA
TACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATAT
GTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGC
AAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGA
ACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATT
CATAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGA
CCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGG
CTGGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGG
ATTCGATGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAA
TAAGCAAATGGAGTTCATGTGGAGATCGACCCAAAGTACACCAXXXXXXXXXXAACCAAC
CAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATT
ACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATT
GGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAAT
GAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTA
TGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTT
TACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACC
ATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGA
TAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGA
TTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAAT
CACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATT
AAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGA
AGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTATAATT
ACTAC

IKKSKPKLSSTLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLA
NPERKFIYVEIAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTID
QMTLGHQFLWENFGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGRMDYQDIEARLEN
KQMEFMWRSTQSTP---

---PTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRG
VGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQ
GVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIF
STITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQCKS
ELL*ll

Frame A:
y*kiktktiiniikcsyccsys**cwlvkns**illwikyvncicrcsiyfgyshylfig
ksrkkvylc*ncifpkmvg*tkynnakyc*gfg*kwsigihkwwvlye**gyyll*ryy*
pndigpsiplgefrsyakdrlahrsiwp*cntssylwsirircihyrsyglsryrssfre
*angvhveidpkyt---

---nqpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi**civvl*kmmvv
vlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigilni
kvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifqls
fqqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqlkkilqrfcfyffsnan
qnfynyy

Frame B:
IKKSKPKLSSTLLNVHIVAHTHDDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLA
NPERKFIYVEIAFFQRWWDEQSTTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTID
QMTLGHQFLWENFGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGRMDYQDIEARLEN
KQMEFMWRSTQSTP---

---tnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddassyfer*wsw
cwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefsis
rclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfnyl
fnnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiin*rrffkgfvftfspmqi
rtfiit

Frame C:
lknqnqnyhqhy*mfilllilmmmlvg*kqlmniimdqicqlhlqvfnilwiqplpvywq
iqkeslfmlklhfskdggmnkvqqckillrvwlkvvnwns*mvgiv*mmrllptmtillt
k*hwainsfgriselcqr*agt*ihlaivqhklvslvn*dsmhslsvvwiikisklv*ri
skwsscgdrpkvh---

---PTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDGRG
VGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQYQ
GVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIF
STITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQCKS
ELL*ll

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA293 (VHA293Q) /CSM/VH/VHA2-D/VHA293Q.Seq.d/                       2567   0.0
VHI312 (VHI312Q) /CSM/VH/VHI3-A/VHI312Q.Seq.d/                       1451   0.0
VHE264 (VHE264Q) /CSM/VH/VHE2-C/VHE264Q.Seq.d/                       1451   0.0
AHL203 (AHL203Q) /CSM/AH/AHL2-A/AHL203Q.Seq.d/                       1451   0.0
AHF510 (AHF510Q) /CSM/AH/AHF5-A/AHF510Q.Seq.d/                       1451   0.0
AHE154 (AHE154Q) /CSM/AH/AHE1-C/AHE154Q.Seq.d/                       1449   0.0
AHC524 (AHC524Q) /CSM/AH/AHC5-A/AHC524Q.Seq.d/                       1449   0.0
SHG288 (SHG288Q) /CSM/SH/SHG2-D/SHG288Q.Seq.d/                       1447   0.0
AHK566 (AHK566Q) /CSM/AH/AHK5-C/AHK566Q.Seq.d/                       1447   0.0
VHD387 (VHD387Q) /CSM/VH/VHD3-D/VHD387Q.Seq.d/                       1441   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1314   0.0    3
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   1e-23  3
DN814237|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-16  4
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-16  4
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   3e-16  4
DN816232| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence.    52   3e-16  4
DN811862| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   3e-16  4
DT613126|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   3e-16  4
DN815247| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   3e-16  4
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   2e-15  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   385   e-105
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   375   e-102
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   232   2e-59
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   231   3e-59
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   231   3e-59
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   231   4e-59
BC091843_1(BC091843|pid:none) Danio rerio zgc:110815, mRNA (cDNA...   230   7e-59
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   230   1e-58
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   230   1e-58
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   230   1e-58

 psg: 0.84  gvh: 0.43  alm: 0.41  top: 0.33  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VHA293 is cyt

>VHA293F.Seq
TATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCA
TACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATAT
GTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGC
AAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGA
ACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATT
CATAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGA
CCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGG
CTGGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGG
ATTCGATGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAA
TAAGCAAATGGAGTTCATGTGGAGATCGACCCAAAGTACACCANNNNNNNNNN

>VHA293Z.Seq
NNNNNNNNNNAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAA
GATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCG
TTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGT
GGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTC
CATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACAT
CCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATAT
CAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAA
ACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTAT
CAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATC
TTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTA
TCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCA
TCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAA
TCAGAACTTTTATAATTACTAC

>VHA293P.Seq
TATTAAAAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCA
TACTCATGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATAT
GTCAATTGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGC
AAATCCAGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGA
ACAAAGTACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATT
CATAAATGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGA
CCAAATGACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGG
CTGGCACATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGG
ATTCGATGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAA
TAAGCAAATGGAGTTCATGTGGAGATCGACCCAAAGTACACCA----------AACCAAC
CAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATT
ACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATT
GGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAAT
GAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTA
TGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTT
TACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACC
ATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGA
TAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGA
TTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAAT
CACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATT
AAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGA
AGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTTTATAATT
ACTAC