>VHA237 (VHA237Q) /CSM/VH/VHA2-B/VHA237Q.Seq.d/
XXXXXXXXXXTCGTCCATCATGGAATTTAACCGTGGTTCAACCAACCAGTGGTAATTATG
TACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTG
TCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGC
ATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTC
AAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATT
ATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAAC
AATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCT
CACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATA
CCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCAC
AAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTG
AAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTG
TTGACTGCTCCTCAGTG

---RPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMH
RRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQ
SSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQ
TITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDCSSV

Frame A:
---RPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMH
RRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQ
SSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQ
TITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDCSSV


Frame B:
---vhhgi*pwfnqpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi**ci
vvl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnn
lqtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihk
lsl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngklltapq


Frame C:
---simefnrgstnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddas
syfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhsti
fkrlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstn
yhfrsfnylfnnynhfcn*nefnwcskiikfikikmenc*llls

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA237 (VHA237Q) /CSM/VH/VHA2-B/VHA237Q.Seq.d/                       1322   0.0
VHM887 (VHM887Q) /CSM/VH/VHM8-D/VHM887Q.Seq.d/                       1298   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1296   0.0
VHP677 (VHP677Q) /CSM/VH/VHP6-D/VHP677Q.Seq.d/                       1296   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1296   0.0
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                       1296   0.0
VHO880 (VHO880Q) /CSM/VH/VHO8-D/VHO880Q.Seq.d/                       1296   0.0
VHO790 (VHO790Q) /CSM/VH/VHO7-D/VHO790Q.Seq.d/                       1296   0.0
VHO689 (VHO689Q) /CSM/VH/VHO6-D/VHO689Q.Seq.d/                       1296   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1296   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1273   0.0    1
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   0.096  8
AP007341|AP007341.1 Lotus japonicus genomic DNA, chromosome 2, clone:LjT35G04, TM0425.    40   0.11   2
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   1.2    1
AM025232|AM025232.1 Bos indicus 3' EST, clone C0005915l21.         34   3.2    2
AC147846|AC147846.2 Otolemur garnettii clone CH256-361F16, WORKING DRAFT SEQUENCE, 3 ordered pieces.    38   3.3    6
BH703301|BH703301.1 BOMNG55TR BO_2_3_KB Brassica oleracea genomic clone BOMNG55, DNA sequence.    40   3.5    2
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   4.2    2
EH243429|EH243429.1 JGI_ACAC13551.fwd ACAC Phakopsora pachyrhizi TW72-1 germinating urediniospores on water surface (20C/16 hours/dark) Phakopsora pachyrhizi cDNA clone ACAC13551 5', mRNA sequence.    44   4.9    1
AV262947|AV262947.1 Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930432P20, 3' end partial sequence.    44   4.9    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   370   e-101
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   370   e-101
BT036325_1(BT036325|pid:none) Zea mays full-length cDNA clone ZM...   100   4e-20
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...    98   3e-19
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...    97   4e-19
EF146940_1(EF146940|pid:none) Populus trichocarpa clone WS01225_...    94   3e-18
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...    94   5e-18
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...    93   8e-18
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...    93   8e-18
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...    93   8e-18

 psg: 0.82  gvh: 0.51  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VHA237 is nuc

>VHA237Z.Seq
NNNNNNNNNNTCGTCCATCATGGAATTTAACCGTGGTTCAACCAACCAGTGGTAATTATG
TACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTG
TCACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGC
ATCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTC
AAATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATT
ATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAAC
AATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCT
CACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATA
CCATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCAC
AAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTG
AAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTG
TTGACTGCTCCTCAGTG