>VHA227 (VHA227Q) /CSM/VH/VHA2-B/VHA227Q.Seq.d/
XXXXXXXXXXTATAATTTCAAATAGTAGTGTACAAGGTAGTGGTGTAATTAATTCATCAA
TTTCATCAAATGGTGGATCAAGTTATGGTAACGATACAATCACAAACTTGGATATTAATG
GTAATTTAGAATCAAATGAAGATGAATTTATATTTACATTGGATGGTAAAAACTCTACAA
CTATTAATATTGGTAATAATGCAACCATTACAAATGCTACTTTAATCATTAGAGTTAGTA
ATGATATTGTAAATTCAACCCAAAATACAACATTGGTAACAAGTTCAAATTTGGATGCAA
CCTTTTCAAAAATTGTTTTTCAATCATATGATCCAGAAACTGGAATTCAAAAACAATTGG
ATGATTGTGCTCATAAAATTAAAAATGATAAAGGTACTCTTGGATTATTATTAAATTCAA
ATGCTAAACAATGTGTAATAGTTTCCACTACAAGTAGTGGTACAGGTGGTGGCACAGGTG
GTGGTACAGGTGGTAGTACAGGTGGTGGTACAAGTGGTAGTACAGGTGGAGGTACAGGTG
GTACAGGTGGAGGTACAGGCCAAACTGTTGTGGTTGTTGAAGAAGATCAAAAGAGCTCAT
TAAGTGCTGGTGCAATTGCTGGTATTATTGTAGGTATCGTTTGTGTTGCTGGTATTGTTG
GTGTAAT

---IISNSSVQGSGVINSSISSNGGSSYGNDTITNLDINGNLESNEDEFIFTLDGKNSTT
INIGNNATITNATLIIRVSNDIVNSTQNTTLVTSSNLDATFSKIVFQSYDPETGIQKQLD
DCAHKIKNDKGTLGLLLNSNAKQCVIVSTTSSGTGGGTGGGTGGSTGGGTSGSTGGGTGG
TGGGTGQTVVVVEEDQKSSLSAGAIAGIIVGIVCVAGIVGV

Frame A:
---IISNSSVQGSGVINSSISSNGGSSYGNDTITNLDINGNLESNEDEFIFTLDGKNSTT
INIGNNATITNATLIIRVSNDIVNSTQNTTLVTSSNLDATFSKIVFQSYDPETGIQKQLD
DCAHKIKNDKGTLGLLLNSNAKQCVIVSTTSSGTGGGTGGGTGGSTGGGTSGSTGGGTGG
TGGGTGQTVVVVEEDQKSSLSAGAIAGIIVGIVCVAGIVGV


Frame B:
---*fqivvykvvv*lihqfhqmvdqvmvtiqsqtwilmvi*nqmkmnlylhwmvktlql
lilvimqplqmll*slelvmil*iqpkiqhw*qvqiwmqpfqklffnhmiqklefknnwm
ivliklkmikvlldyy*iqmlnnv**fplqvvvqvvaqvvvqvvvqvvvqvvvqvevqvv
qvevqakllwllkkikrah*vlvqllvll*vsfvllvllv*


Frame C:
---nfk**ctr*wcn*finfikwwiklw*rynhklgy*w*frik*r*iyiyigw*klyny
*yw**cnhykcyfnh*s***yckfnpkynignkfkfgcnlfkncfsii*srnwnsktig*
lcs*n*k**ryswiiikfkc*tmcnsfhyk*wyrwwhrwwyrw*yrwwykw*yrwryrwy
rwryrpnccgc*rrskelikcwcncwyycryrlccwycwcn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL427 (VHL427Q) /CSM/VH/VHL4-B/VHL427Q.Seq.d/                        884   0.0
VHK596 (VHK596Q) /CSM/VH/VHK5-D/VHK596Q.Seq.d/                        884   0.0
VHH794 (VHH794Q) /CSM/VH/VHH7-D/VHH794Q.Seq.d/                        884   0.0
VHH521 (VHH521Q) /CSM/VH/VHH5-A/VHH521Q.Seq.d/                        884   0.0
VHE339 (VHE339Q) /CSM/VH/VHE3-B/VHE339Q.Seq.d/                        884   0.0
VHD329 (VHD329Q) /CSM/VH/VHD3-B/VHD329Q.Seq.d/                        884   0.0
VHC580 (VHC580Q) /CSM/VH/VHC5-D/VHC580Q.Seq.d/                        884   0.0
VHA340 (VHA340Q) /CSM/VH/VHA3-B/VHA340Q.Seq.d/                        884   0.0
VHA227 (VHA227Q) /CSM/VH/VHA2-B/VHA227Q.Seq.d/                        884   0.0
VHJ262 (VHJ262Q) /CSM/VH/VHJ2-C/VHJ262Q.Seq.d/                        876   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ682951|AZ682951.1 ENTHL32TR Entamoeba histolytica Sheared DNA Entamoeba histolyticagenomic, genomic survey sequence.    54   0.005  1
AZ541475|AZ541475.1 ENTGK77TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    54   0.005  1
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    32   0.013  9
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    38   0.057  10
AM479829|AM479829.1 Vitis vinifera, whole genome shotgun sequence, contig  VV78X007751.19, clone ENTAV 115.    42   0.18   5
AZ679768|AZ679768.1 ENTJB51TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38   0.23   2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    48   0.31   1
AC124970|AC124970.21 Medicago truncatula clone mth2-7j19, complete sequence.    38   0.56   5
AB032076|AB032076.1 Dictyostelium discoideum mRNA for lagC2, complete cds.    40   0.72   3
DR777729|DR777729.1 BAAC-PNP1255A15.g1 C.remanei EST SB146 Caenorhabditis remanei cDNA 5, mRNA sequence.    46   1.2    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000568_3082(CP000568|pid:none) Clostridium thermocellum ATCC 2...    34   4.4
CP000647_1382(CP000647|pid:none) Klebsiella pneumoniae subsp. pn...    34   4.4
AC116957_68(AC116957|pid:none) Dictyostelium discoideum chromoso...    33   7.5
AY698035_1(AY698035|pid:none) Desmognathus marmoratus isolate 69...    33   7.5
AY612348_1(AY612348|pid:none) Desmognathus quadramaculatus vouch...    33   9.9
AY698038_1(AY698038|pid:none) Desmognathus marmoratus isolate T0...    33   9.9
AY612344_1(AY612344|pid:none) Desmognathus marmoratus voucher KH...    33   9.9
AY698041_1(AY698041|pid:none) Desmognathus marmoratus isolate T0...    33   9.9
DQ018668_1(DQ018668|pid:none) Plethodon hubrichti isolate 75144 ...    33   9.9

 psg: 0.83  gvh: 0.39  alm: 0.16  top: 0.67  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHA227 is cyt

>VHA227Z.Seq
NNNNNNNNNNTATAATTTCAAATAGTAGTGTACAAGGTAGTGGTGTAATTAATTCATCAA
TTTCATCAAATGGTGGATCAAGTTATGGTAACGATACAATCACAAACTTGGATATTAATG
GTAATTTAGAATCAAATGAAGATGAATTTATATTTACATTGGATGGTAAAAACTCTACAA
CTATTAATATTGGTAATAATGCAACCATTACAAATGCTACTTTAATCATTAGAGTTAGTA
ATGATATTGTAAATTCAACCCAAAATACAACATTGGTAACAAGTTCAAATTTGGATGCAA
CCTTTTCAAAAATTGTTTTTCAATCATATGATCCAGAAACTGGAATTCAAAAACAATTGG
ATGATTGTGCTCATAAAATTAAAAATGATAAAGGTACTCTTGGATTATTATTAAATTCAA
ATGCTAAACAATGTGTAATAGTTTCCACTACAAGTAGTGGTACAGGTGGTGGCACAGGTG
GTGGTACAGGTGGTAGTACAGGTGGTGGTACAAGTGGTAGTACAGGTGGAGGTACAGGTG
GTACAGGTGGAGGTACAGGCCAAACTGTTGTGGTTGTTGAAGAAGATCAAAAGAGCTCAT
TAAGTGCTGGTGCAATTGCTGGTATTATTGTAGGTATCGTTTGTGTTGCTGGTATTGTTG
GTGTAAT