>VHA196 (VHA196Q) /CSM/VH/VHA1-D/VHA196Q.Seq.d/
GGCCTACTGGGATGAAGTTAATTCAATTTTAGATTTATGTTCACATGGTAGTGGTAGTGG
TGGTAGTGATGGTAATACTAGAGGTAGTGCTAATGAAAAGATTAAAGTATCACCAGAATT
AGGTAATGTTATTTTTGCAAGTTCAGAAATGGGTTGGTGTTTTAGTTTAGAATCATTCGC
AAATATTTATATTGAAACTTATGGTGGTGGTTTTAAGAGTAGTGAATTTGCTAAAAGATT
ATGGGGTGATCTTTATTTTAATGAAAGTACACGTATGTTTAGAAAACAAGCATCAGGAAA
TGGTGAACATGTAAGATCATTTGTACACTTTATTTTAAATCCAATTTATAAGATTTATTC
AACTGTAATTAGTGAAGAGAAACCAGTGGTTGAAGCAACCCTAAGAGAATTGGGTATTAA
ACTTTCAAAAGAAACTTATAATTTAGATATTAGACCATTGTTAAGAGTTGTATTGGGTTC
ATTTTTCGGTGGTAAATCAAATGCATTTGTAGATATGTTGGCAGCCTTACCATCACCAAT
TGATAATXXXXXXXXXXTCCAATTCGATTTGGGCATTTGGTCCAGATATCAATGGTCCAA
ATACCCTATTGAACGATACCCTCCCAACTGAAGTGAACAGATCCCTTCTATTATCCATCT
CTGATTCCGTGGTTAGAGGCTTCCAATGGGCCACTAAAGAAGGTCCATTGGTTGATGAAC
CAATTAGAAATGTAAAATTCAAACTATTGGATGCCACCATTGCCTCTGAACCAATTCAAA
GATCCAGTGGCCATATTGTACCTGCCGCACGTTCTGTAACACATTCATCATTTTTAGTAT
CAACACCACGTTTAATGGAGCCTGTTTATCTAGTTGAAGTAATCTCACCAATCGATTGTC
TCAATGCAATCGAAAATGTTTTAACACGTCGTCGTGGTCATATCATTCATGACTTGCCAA
AACCTGGTACACCACTTCACATTACAAAAGCTTTAATACCCGTATTGGATTCATATGGTT
TTGAAACCGATCTTAGAGTTCATACTCAAGGTCAAGCTTTCTGTCTTTCAACATTTGATC
ATTGGCAAGTGGTTCCAGGTGATCCTTTAGATAAATCAATTAAACTTCGTCCATTAGAAC
CTTCTCCAAAACTTCATTTGGCTCGTGAACTTTTAATCAAAACTCGTAAGAGAAAAGGTC
TATCTGAAGATGTTAATTTTGGTAAACATTTCGAGAACAACTTTTATTATCTTTATCTCA
AAANAAAATCAAT

AYWDEVNSILDLCSHGSGSGGSDGNTRGSANEKIKVSPELGNVIFASSEMGWCFSLESFA
NIYIETYGGGFKSSEFAKRLWGDLYFNESTRMFRKQASGNGEHVRSFVHFILNPIYKIYS
TVISEEKPVVEATLRELGIKLSKETYNLDIRPLLRVVLGSFFGGKSNAFVDMLAALPSPI
DN---

---SNSIWAFGPDINGPNTLLNDTLPTEVNRSLLLSISDSVVRGFQWATKEGPLVDEPIR
NVKFKLLDATIASEPIQRSSGHIVPAARSVTHSSFLVSTPRLMEPVYLVEVISPIDCLNA
IENVLTRRRGHIIHDLPKPGTPLHITKALIPVLDSYGFETDLRVHTQGQAFCLSTFDHWQ
VVPGDPLDKSIKLRPLEPSPKLHLARELLIKTRKRKGLSEDVNFGKHFENNFYYLYLKXK
S

Frame A:
gllg*s*fnfrfmftw*w*ww**w*y*r*c**kd*sitrir*cyfckfrnglvf*friir
kyly*nlwwwf*e**ic*kimg*slf**kytyv*ktsirkw*tckiictlyfksnl*dlf
ncn**retsg*snpkrigy*tfkrnl*fry*tivkscigfifrw*ikcicryvgsltitn
**---

---SNSIWAFGPDINGPNTLLNDTLPTEVNRSLLLSISDSVVRGFQWATKEGPLVDEPIR
NVKFKLLDATIASEPIQRSSGHIVPAARSVTHSSFLVSTPRLMEPVYLVEVISPIDCLNA
IENVLTRRRGHIIHDLPKPGTPLHITKALIPVLDSYGFETDLRVHTQGQAFCLSTFDHWQ
VVPGDPLDKSIKLRPLEPSPKLHLARELLIKTRKRKGLSEDVNFGKHFENNFYYLYLKXK
S

Frame B:
AYWDEVNSILDLCSHGSGSGGSDGNTRGSANEKIKVSPELGNVIFASSEMGWCFSLESFA
NIYIETYGGGFKSSEFAKRLWGDLYFNESTRMFRKQASGNGEHVRSFVHFILNPIYKIYS
TVISEEKPVVEATLRELGIKLSKETYNLDIRPLLRVVLGSFFGGKSNAFVDMLAALPSPI
DN---

---pirfghlvqismvqipy*tipsqlk*tdpfyypslipwleasngplkkvhwlmnqle
m*nsnywmpplplnqfkdpvailylphvl*hihhf*yqhhv*wslfi*lk*shqsivsmq
skmf*hvvvvisfmtcqnlvhhftlqkl*ypywihmvlkpilefilkvklsvfqhliigk
wfqvil*inqlnfvh*nllqnfiwlvnf*sklvrekvylkmlilvnisrttfiifiskxn
q

Frame C:
ptgmkliqf*iyvhmvvvvvvvmvilevvlmkrlkyhqn*vmlflqvqkwvgvlv*nhsq
ifilklmvvvlrvvnllkdygvifilmkvhvclenkhqemvnm*dhlytlf*iqfirfiq
l*lvkrnqwlkqp*enwvlnfqkklii*ildhc*elywvhfsvvnqmhl*icwqpyhhql
i---

---qfdlgiwsryqwskypieryppn*seqipsiihl*frg*rlpmgh*rrsig**tn*k
ckiqtigchhcl*tnskiqwpyctcrtfcntfiifsinttfngaclss*snltnrlsqcn
rkcfntsswsyhs*laktwyttshyksfntrigfiwf*nrs*ssysrssflsfni*slas
gsr*sfr*in*tssirtfsktsfgs*tfnqns*ekrsi*rc*fw*tfreqlllslsqxki
n

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA196 (VHA196Q) /CSM/VH/VHA1-D/VHA196Q.Seq.d/                       2458   0.0
SHC595 (SHC595Q) /CSM/SH/SHC5-D/SHC595Q.Seq.d/                       1402   0.0
AHA520 (AHA520Q) /CSM/AH/AHA5-A/AHA520Q.Seq.d/                       1402   0.0
CHM739 (CHM739Q) /CSM/CH/CHM7-B/CHM739Q.Seq.d/                       1390   0.0
CHM277 (CHM277Q) /CSM/CH/CHM2-D/CHM277Q.Seq.d/                       1390   0.0
AHB170 (AHB170Q) /CSM/AH/AHB1-C/AHB170Q.Seq.d/                       1384   0.0
VHK512 (VHK512Q) /CSM/VH/VHK5-A/VHK512Q.Seq.d/                       1382   0.0
AHK120 (AHK120Q) /CSM/AH/AHK1-A/AHK120Q.Seq.d/                       1378   0.0
AHL644 (AHL644Q) /CSM/AH/AHL6-B/AHL644Q.Seq.d/                       1376   0.0
AHK366 (AHK366Q) /CSM/AH/AHK3-C/AHK366Q.Seq.d/                       1364   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

DY895165|DY895165.1 CeleSEQ14922 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ14922, mRNA sequence.    50   1e-15  5
AJ821374|AJ821374.1 Theileria annulata EST, clone C0005205b23.     48   3e-13  5
DY890501|DY890501.1 CeleSEQ9529 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ9529, mRNA sequence.    50   6e-11  2
DY891922|DY891922.1 CeleSEQ8678 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ8678, mRNA sequence.    50   6e-11  2
EE117765|EE117765.1 G709P5182RF12.T0 Acorn worm normalized neurula pExpress1 library Saccoglossus kowalevskii cDNA 5' end, mRNA sequence.    40   1e-09  4
DV618751|DV618751.1 EST1221747 Glossina morsitans morsitans Fat body cDNA Glossina morsitans morsitans cDNA clone GLAET57 3' end similar to (Q9VAX8) CG4849-PA (LD28793p), mRNA sequence.    74   6e-09  2
BI742991|BI742991.1 kx37e02.y1 Parastrongyloides trichosuri IL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to TR:Q19070 Q19070 ELONGATION FACTOR 2-LIKE ;, mRNA sequence.    70   9e-09  2
BI743756|BI743756.1 kx52f02.y1 Parastrongyloides trichosuri PA pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to TR:O08810 O08810 U5-116KD. ;, mRNA sequence.    70   9e-09  2
BI501287|BI501287.1 kx30d02.y1 Parastrongyloides trichosuri PA pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to TR:O08810 O08810 U5-116KD. ;, mRNA sequence.    70   1e-08  2
BI743844|BI743844.1 kx53f10.y1 Parastrongyloides trichosuri PA pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to TR:O08810 O08810 U5-116KD. ;, mRNA sequence.    70   1e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK221352_1(AK221352|pid:none) Arabidopsis thaliana mRNA for elon...   333   8e-90
AK102908_1(AK102908|pid:none) Oryza sativa Japonica Group cDNA c...   323   6e-87
BC090572_1(BC090572|pid:none) Xenopus tropicalis eftud2 protein,...   321   3e-86
BC044041_1(BC044041|pid:none) Xenopus laevis similar to U5 snRNP...   321   3e-86
BC041724_1(BC041724|pid:none) Xenopus laevis U5 snRNP-specific p...   321   4e-86
(Q15029) RecName: Full=116 kDa U5 small nuclear ribonucleoprotei...   320   5e-86
CR456774_1(CR456774|pid:none) Homo sapiens full open reading fra...   320   5e-86
AK172875_1(AK172875|pid:none) Mus musculus mRNA for mKIAA0031 pr...   320   5e-86
BC002360_1(BC002360|pid:none) Homo sapiens elongation factor Tu ...   320   5e-86
(A4FUD3) RecName: Full=116 kDa U5 small nuclear ribonucleoprotei...   320   5e-86

 psg: 0.75  gvh: 0.57  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VHA196 is cyt

>VHA196F.Seq
GGCCTACTGGGATGAAGTTAATTCAATTTTAGATTTATGTTCACATGGTAGTGGTAGTGG
TGGTAGTGATGGTAATACTAGAGGTAGTGCTAATGAAAAGATTAAAGTATCACCAGAATT
AGGTAATGTTATTTTTGCAAGTTCAGAAATGGGTTGGTGTTTTAGTTTAGAATCATTCGC
AAATATTTATATTGAAACTTATGGTGGTGGTTTTAAGAGTAGTGAATTTGCTAAAAGATT
ATGGGGTGATCTTTATTTTAATGAAAGTACACGTATGTTTAGAAAACAAGCATCAGGAAA
TGGTGAACATGTAAGATCATTTGTACACTTTATTTTAAATCCAATTTATAAGATTTATTC
AACTGTAATTAGTGAAGAGAAACCAGTGGTTGAAGCAACCCTAAGAGAATTGGGTATTAA
ACTTTCAAAAGAAACTTATAATTTAGATATTAGACCATTGTTAAGAGTTGTATTGGGTTC
ATTTTTCGGTGGTAAATCAAATGCATTTGTAGATATGTTGGCAGCCTTACCATCACCAAT
TGATAATNNNNNNNNNN

>VHA196Z.Seq
NNNNNNNNNNTCCAATTCGATTTGGGCATTTGGTCCAGATATCAATGGTCCAAATACCCT
ATTGAACGATACCCTCCCAACTGAAGTGAACAGATCCCTTCTATTATCCATCTCTGATTC
CGTGGTTAGAGGCTTCCAATGGGCCACTAAAGAAGGTCCATTGGTTGATGAACCAATTAG
AAATGTAAAATTCAAACTATTGGATGCCACCATTGCCTCTGAACCAATTCAAAGATCCAG
TGGCCATATTGTACCTGCCGCACGTTCTGTAACACATTCATCATTTTTAGTATCAACACC
ACGTTTAATGGAGCCTGTTTATCTAGTTGAAGTAATCTCACCAATCGATTGTCTCAATGC
AATCGAAAATGTTTTAACACGTCGTCGTGGTCATATCATTCATGACTTGCCAAAACCTGG
TACACCACTTCACATTACAAAAGCTTTAATACCCGTATTGGATTCATATGGTTTTGAAAC
CGATCTTAGAGTTCATACTCAAGGTCAAGCTTTCTGTCTTTCAACATTTGATCATTGGCA
AGTGGTTCCAGGTGATCCTTTAGATAAATCAATTAAACTTCGTCCATTAGAACCTTCTCC
AAAACTTCATTTGGCTCGTGAACTTTTAATCAAAACTCGTAAGAGAAAAGGTCTATCTGA
AGATGTTAATTTTGGTAAACATTTCGAGAACAACTTTTATTATCTTTATCTCAAAANAAA
ATCAAT

>VHA196P.Seq
GGCCTACTGGGATGAAGTTAATTCAATTTTAGATTTATGTTCACATGGTAGTGGTAGTGG
TGGTAGTGATGGTAATACTAGAGGTAGTGCTAATGAAAAGATTAAAGTATCACCAGAATT
AGGTAATGTTATTTTTGCAAGTTCAGAAATGGGTTGGTGTTTTAGTTTAGAATCATTCGC
AAATATTTATATTGAAACTTATGGTGGTGGTTTTAAGAGTAGTGAATTTGCTAAAAGATT
ATGGGGTGATCTTTATTTTAATGAAAGTACACGTATGTTTAGAAAACAAGCATCAGGAAA
TGGTGAACATGTAAGATCATTTGTACACTTTATTTTAAATCCAATTTATAAGATTTATTC
AACTGTAATTAGTGAAGAGAAACCAGTGGTTGAAGCAACCCTAAGAGAATTGGGTATTAA
ACTTTCAAAAGAAACTTATAATTTAGATATTAGACCATTGTTAAGAGTTGTATTGGGTTC
ATTTTTCGGTGGTAAATCAAATGCATTTGTAGATATGTTGGCAGCCTTACCATCACCAAT
TGATAAT----------TCCAATTCGATTTGGGCATTTGGTCCAGATATCAATGGTCCAA
ATACCCTATTGAACGATACCCTCCCAACTGAAGTGAACAGATCCCTTCTATTATCCATCT
CTGATTCCGTGGTTAGAGGCTTCCAATGGGCCACTAAAGAAGGTCCATTGGTTGATGAAC
CAATTAGAAATGTAAAATTCAAACTATTGGATGCCACCATTGCCTCTGAACCAATTCAAA
GATCCAGTGGCCATATTGTACCTGCCGCACGTTCTGTAACACATTCATCATTTTTAGTAT
CAACACCACGTTTAATGGAGCCTGTTTATCTAGTTGAAGTAATCTCACCAATCGATTGTC
TCAATGCAATCGAAAATGTTTTAACACGTCGTCGTGGTCATATCATTCATGACTTGCCAA
AACCTGGTACACCACTTCACATTACAAAAGCTTTAATACCCGTATTGGATTCATATGGTT
TTGAAACCGATCTTAGAGTTCATACTCAAGGTCAAGCTTTCTGTCTTTCAACATTTGATC
ATTGGCAAGTGGTTCCAGGTGATCCTTTAGATAAATCAATTAAACTTCGTCCATTAGAAC
CTTCTCCAAAACTTCATTTGGCTCGTGAACTTTTAATCAAAACTCGTAAGAGAAAAGGTC
TATCTGAAGATGTTAATTTTGGTAAACATTTCGAGAACAACTTTTATTATCTTTATCTCA
AAANAAAATCAAT