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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHA192
Library VH
(Link to library)
Clone ID VHA192
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHA192P
(Link to Original site)
Representative DNA sequence 
>VHA192 (VHA192Q) /CSM/VH/VHA1-D/VHA192Q.Seq.d/
ATCATTATTGTTAAAATAAGAGTAGTAAGTTAACTTCATTAATATTAAATAATTAAATAT
TAAAAAATGTCATCAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGG
CAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTAT
GTTGCATTTACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATG
AATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAA
GAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAG
CCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCT
TCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAAT
AATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGAT
GCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCT
ATTGCTTATGGTTXXXXXXXXXXAGTGTACTCCAAATGGGTTAGAATCCAAATCAAACCG
CAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCA
TGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGT
CAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTT
CAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAAATAATAAGTTAGTAAAATCAA
TAAAGCTTCTCATATA
sequence update 2002.10.25
Translated Amino Acid sequence 
iiivkirvvs*lh*y*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSY
VAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDK
PHIQVEFKGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKD
AGTISKLNVQRIINEPTAAAIAYG---

---VYSKWVRIQIKPQKRMNMKIR*kp*kqlsiqs*vnsikkevchkvvvcqvvcqmihq
rvqttk*tn*idgifqrktslsnrflkkiis**nq*sfsy
Translated Amino Acid sequence (All Frames) 
Frame A:
iiivkirvvs*lh*y*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSY
VAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDK
PHIQVEFKGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKD
AGTISKLNVQRIINEPTAAAIAYG---

---svlqmg*npnqtaekdeyedkmkaleavvnpimsklyqeggmpqgggmpggmsndsp
kssnnkvdeld*rnfseenisik*is*knnklvksiklli

Frame B:
slllk*e**vnfinik*lnikkchqlvli*vqhihvlvfgkmivlkllqmikvieplhlm
lhlliqnv*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvis
hifklnskvklkfshqkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkm
lvqfqn*mfnvllmnqllllllmv---

---VYSKWVRIQIKPQKRMNMKIR*kp*kqlsiqs*vnsikkevchkvvvcqvvcqmihq
rvqttk*tn*idgifqrktslsnrflkkiis**nq*sfsy

Frame C:
hyc*nksskltslilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsilc
ciy*yrtfnw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**a
tyss*iqr*n*sfltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrc
wynfkikcstyy**tncccyclw---

---ctpnglesksnrrkg*i*r*deslrsscqsnhe*tlsrrryatrwwyarwyvk*ftk
efkqqsrrirlteffrgkhlyqidflkk**vskinkashi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA192 (VHA192Q) /CSM/VH/VHA1-D/VHA192Q.Seq.d/                       1715   0.0
VHF339 (VHF339Q) /CSM/VH/VHF3-B/VHF339Q.Seq.d/                       1203   0.0
CFJ332 (CFJ332Q) /CSM/CF/CFJ3-B/CFJ332Q.Seq.d/                       1193   0.0
VHP558 (VHP558Q) /CSM/VH/VHP5-C/VHP558Q.Seq.d/                       1191   0.0
CHI510 (CHI510Q) /CSM/CH/CHI5-A/CHI510Q.Seq.d/                       1191   0.0
VHO460 (VHO460Q) /CSM/VH/VHO4-C/VHO460Q.Seq.d/                       1187   0.0
VHB518 (VHB518Q) /CSM/VH/VHB5-A/VHB518Q.Seq.d/                       1181   0.0
CFA811 (CFA811Q) /CSM/CF/CFA8-A/CFA811Q.Seq.d/                       1166   0.0
SFG485 (SFG485Q) /CSM/SF/SFG4-D/SFG485Q.Seq.d/                       1162   0.0
VHP603 (VHP603Q) /CSM/VH/VHP6-A/VHP603Q.Seq.d/                       1160   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1057   0.0    3
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.   900   0.0    3
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   285   e-142  2
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   285   e-142  2
EH431862|EH431862.1 NPE00000311 Neocallimastix patriciarum ZAP II cDNA library Neocallimastix patriciarum cDNA, mRNA sequence.   236   8e-99  5
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   145   1e-83  8
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 123   1e-78  7
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   123   3e-78  7
DY886418|DY886418.1 CeleSEQ2066 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ2066, mRNA sequence.   153   4e-78  7
DY886204|DY886204.1 CeleSEQ1763 Cunninghamella elegans pBluescript (EcoRI-XhoI) Cunninghamella elegans cDNA clone CeleSEQ1763, mRNA sequence.   153   4e-78  7
dna update 2007. 3.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   365   1e-99
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   363   6e-99
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   341   2e-92
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   341   2e-92
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           323   4e-87
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   315   1e-84
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   315   1e-84
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   315   1e-84
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   314   3e-84
EF442767_1(EF442767|pid:none) Trypanosoma rangeli isolate H14 cl...   313   5e-84
protein update 2009. 6.26
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHA192 is cyt
5' end seq. ID VHA192F
5' end seq. 
>VHA192F.Seq
ATCATTATTGTTAAAATAAGAGTAGTAAGTTAACTTCATTAATATTAAATAATTAAATAT
TAAAAAATGTCATCAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGG
CAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTAT
GTTGCATTTACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATG
AATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAA
GAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAG
CCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCT
TCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAAT
AATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGAT
GCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCT
ATTGCTTATGGTTNNNNNNNNNN
Length of 5' end seq. 623
3' end seq. ID VHA192Z
3' end seq. 
>VHA192Z.Seq
NNNNNNNNNNAGTGTACTCCAAATGGGTTAGAATCCAAATCAAACCGCAGAAAAGGATGA
ATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTA
TCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACC
AAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACAT
CTCTATCAAATAGATTTCTTAAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCAT
ATA
Length of 3' end seq. 303
Connected seq. ID VHA192P
Connected seq. 
>VHA192P.Seq
ATCATTATTGTTAAAATAAGAGTAGTAAGTTAACTTCATTAATATTAAATAATTAAATAT
TAAAAAATGTCATCAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGG
CAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTAT
GTTGCATTTACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATG
AATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAA
GAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAG
CCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCT
TCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAAT
AATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGAT
GCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCT
ATTGCTTATGGTT----------AGTGTACTCCAAATGGGTTAGAATCCAAATCAAACCG
CAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCA
TGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGT
CAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTT
CAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAAATAATAAGTTAGTAAAATCAA
TAAAGCTTCTCATATA
Length of connected seq. 906
Full length Seq ID -
Full length Seq. -
Length of full length seq. -