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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHA176
Library VH
(Link to library)
Clone ID VHA176
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15241-1
Original site URL
Representative seq. ID VHA176P
(Link to Original site)
Representative DNA sequence 
>VHA176 (VHA176Q) /CSM/VH/VHA1-D/VHA176Q.Seq.d/
GTTCAAGACATATGGAATGCTGATCAAACCTCAACCAACAATTACAAACTCCCAAGTTGG
TTAGGTGAAATCCCTGTAAATGGTGCTCACCAATTTGGTTACATCCAAAGATCAACATCA
CTCACTCCAATAACTGGCACTCCATCTTGTTAAAACAACACAGTAAATAAAATTTTAATT
TACAACTCGCCAAAAAAAAAAXXXXXXXXXXGTTCAAGACATATGGAATGCTGATCAAAC
CTCAACCAACAATTACAAACTCCCAAGTTGGTTAGGTGAAATCCCTGTAAATGGTGCTCA
CCAATTTGGTTACATCCAAAGATCAACATCACTCACTCCAATAACTGCACTCCATCTTGT
TAAAACAACACAGTAAATAAAATTTTAATTTACAACTCG
sequence update 2002.10.25
Translated Amino Acid sequence 
vqdiwnadqtstnnyklpswlgeipvngahqfgyiqrstsltpitgtpsc*NNTVNKILI
YNSPKKK---

---VQDIWNADQTSTNNYKLPSWLGEIPVNGAHQFGYIQRSTSLTPITALHLVKTTQ*ik
f*ftt
Translated Amino Acid sequence (All Frames) 
Frame A:
vqdiwnadqtstnnyklpswlgeipvngahqfgyiqrstsltpitgtpsc*NNTVNKILI
YNSPKKK---

---VQDIWNADQTSTNNYKLPSWLGEIPVNGAHQFGYIQRSTSLTPITALHLVKTTQ*ik
f*ftt

Frame B:
fktygmlikpqptitnsqvg*vksl*mvltnlvtskdqhhslq*lalhlvkttq*ikf*f
ttrqkk---

---fktygmlikpqptitnsqvg*vksl*mvltnlvtskdqhhslq*lhsillkqhsk*n
fnlql

Frame C:
srhmec*snlnqqlqtpklvr*npckwcspiwlhpkinithsnnwhsillkqhsk*nfnl
qlakkk---

---srhmec*snlnqqlqtpklvr*npckwcspiwlhpkinithsnnctpsc*nntvnki
liyns
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA176 (VHA176Q) /CSM/VH/VHA1-D/VHA176Q.Seq.d/                        379   e-104
VSC576 (VSC576Q) /CSM/VS/VSC5-D/VSC576Q.Seq.d/                        371   e-102
SFC154 (SFC154Q) /CSM/SF/SFC1-C/SFC154Q.Seq.d/                        367   e-101
VFA855 (VFA855Q) /CSM/VF/VFA8-C/VFA855Q.Seq.d/                         58   1e-07
VFA208 (VFA208Q) /CSM/VF/VFA2-A/VFA208Q.Seq.d/                         42   0.006
CFK257 (CFK257Q) /CSM/CF/CFK2-C/CFK257Q.Seq.d/                         40   0.025
CFJ136 (CFJ136Q) /CSM/CF/CFJ1-B/CFJ136Q.Seq.d/                         40   0.025
VSA772 (VSA772Q) /CSM/VS/VSA7-C/VSA772Q.Seq.d/                         38   0.098
VSI595 (VSI595Q) /CSM/VS/VSI5-D/VSI595Q.Seq.d/                         36   0.39
VSF263 (VSF263Q) /CSM/VS/VSF2-C/VSF263Q.Seq.d/                         36   0.39
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC129740|AC129740.3 Rattus norvegicus clone CH230-173O1, *** SEQUENCING IN PROGRESS ***, 10 unordered pieces.    36   0.007  4
AM426859|AM426859.1 Vitis vinifera, whole genome shotgun sequence, contig  VV78X184872.25, clone ENTAV 115.    38   0.035  2
CW957947|CW957947.1 TcB53.3_F04_T7 Tribolium BAC library Tribolium castaneum genomic, genomic survey sequence.    30   0.072  4
CW954836|CW954836.1 TcB44.3_D09_SP6 Tribolium BAC library Tribolium castaneum genomic, genomic survey sequence.    30   0.077  4
CG768735|CG768735.1 TcB47.3_H07_SP6 Tribolium BAC library Tribolium castaneum genomic, genomic survey sequence.    30   0.079  4
CW957442|CW957442.1 TcB53.1_A04_SP6 Tribolium BAC library Tribolium castaneum genomic, genomic survey sequence.    30   0.087  4
CG770886|CG770886.1 TcB40.4_B07_T7 Tribolium BAC library Tribolium castaneum genomic, genomic survey sequence.    30   0.090  4
CW947110|CW947110.1 TcB30.2_B10_SP6 Tribolium BAC library Tribolium castaneum genomic, genomic survey sequence.    30   0.095  4
CG771597|CG771597.1 TcB17.1_E12_T7 Tribolium BAC library Tribolium castaneum genomic, genomic survey sequence.    28   0.22   4
CW953789|CW953789.1 TcB40.1_A01_T7 Tribolium BAC library Tribolium castaneum genomic, genomic survey sequence.    28   0.23   4
dna update 2007. 3.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P22698) RecName: Full=Spore germination protein 270-11;  &B3562...    59   5e-08
T18270(T18270) hypothetical protein - slime mold (Dictyostelium ...    55   7e-07
protein update 2009. 2.21
PSORT 

 psg: 0.70  gvh: 0.38  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 20.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall

>> prediction for VHA176 is nuc
5' end seq. ID VHA176F
5' end seq. 
>VHA176F.Seq
GTTCAAGACATATGGAATGCTGATCAAACCTCAACCAACAATTACAAACTCCCAAGTTGG
TTAGGTGAAATCCCTGTAAATGGTGCTCACCAATTTGGTTACATCCAAAGATCAACATCA
CTCACTCCAATAACTGGCACTCCATCTTGTTAAAACAACACAGTAAATAAAATTTTAATT
TACAACTCGCCAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 211
3' end seq. ID VHA176Z
3' end seq. 
>VHA176Z.Seq
NNNNNNNNNNGTTCAAGACATATGGAATGCTGATCAAACCTCAACCAACAATTACAAACT
CCCAAGTTGGTTAGGTGAAATCCCTGTAAATGGTGCTCACCAATTTGGTTACATCCAAAG
ATCAACATCACTCACTCCAATAACTGCACTCCATCTTGTTAAAACAACACAGTAAATAAA
ATTTTAATTTACAACTCG
Length of 3' end seq. 198
Connected seq. ID VHA176P
Connected seq. 
>VHA176P.Seq
GTTCAAGACATATGGAATGCTGATCAAACCTCAACCAACAATTACAAACTCCCAAGTTGG
TTAGGTGAAATCCCTGTAAATGGTGCTCACCAATTTGGTTACATCCAAAGATCAACATCA
CTCACTCCAATAACTGGCACTCCATCTTGTTAAAACAACACAGTAAATAAAATTTTAATT
TACAACTCGCCAAAAAAAAAA----------GTTCAAGACATATGGAATGCTGATCAAAC
CTCAACCAACAATTACAAACTCCCAAGTTGGTTAGGTGAAATCCCTGTAAATGGTGCTCA
CCAATTTGGTTACATCCAAAGATCAACATCACTCACTCCAATAACTGCACTCCATCTTGT
TAAAACAACACAGTAAATAAAATTTTAATTTACAACTCG
Length of connected seq. 389
Full length Seq ID -
Full length Seq. -
Length of full length seq. -