>VHA155 (VHA155Q) /CSM/VH/VHA1-C/VHA155Q.Seq.d/
TAATATTTAAAAAGATNGATAAAAATAAATAATTAATCANTTAATAAAAAAAAANTANTA
AAAAAAAAAAAAGGTAAANNAAAAGGTAAAANNNGTAATTTANNANTANNAAGGCCAAAT
TTAANNANNAAAXXXXXXXXXXANGCATCAAGCTCNCCAATTTCAAGTGGTGGNTNTGAT
CCAATGCAAATTAAGATTTTAGCATCATACAAGAGATTATGTTTAGTTAGTGAGGGGTGT
ACTCTATGAGGATAGTATGTTACAAGTTGGTTTGAAATCAGAATATCAATCAGGTCAAGG
TAGATTAATGTTATACTATGGAAATAGTTCAGCATTCCCATTGACCAATTTCAATGTTAC
CCTTAATTCAATCGCTGGTTTAACATTACAACCACAATCGATTGCACCAGTTATTCAACC
AAAGGCACAATTACAACAACCAGTCACTTTTTCATGTACATCAGAGTTTACTGAATCACC
AGTTATCACCATTAATTTCCTTACACCAGGTAAACCAATCACTATCACCCTTCGTCTTCC
AATTGTTATCAGTAAATTCTTTGAACCATTACGTTTATCAAGTGGTGACTTTTTCGCTCG
TTGGAAGACAATCTCTGGTAAACCATTGGAAATTCAAGAAATCTTTAAATCAACTAAACC
AATCGATATTCAATCATACAATAGAGTCATTCAAGAAGGTTTAAATATTACCGTACTCAA
ACAGGTAGATCCAAATCCAAATAATATTGTAGCATCTTGTCTCTTCCCATTCGGTTCAAA
TGGTCAACCAATAAACTNTTATATTAGAATTGAAACCAACCCACAAGCAAATATGTGTCG
TCTTACTATTAG

*ylkrxikinn*SXNKKKXXKKKKGKXKGKXXNLXXXRPNLXXK---

---ASSSPISSGGXDPMQIKILASYKRLCLVSEGCTL*g*yvtswfeirisirsr*invi
lwk*fsipidqfqcyp*fnrwfnitttidctsystkgtitttshffmyirvy*itsyhh*
fpytr*tnhyhpsssncyq*il*titfikw*lfrslednlw*tignsrnl*in*tnrysi
iq*shsrrfkyyrtqtgrsksk*ycsilslpirfkwstnklly*n*nqptskyvssyy*

Frame A:
*ylkrxikinn*SXNKKKXXKKKKGKXKGKXXNLXXXRPNLXXK---

---xhqaxqfqvvxxiqcklrf*hhtrdyv*lvrgvlyedsmlqvglkseyqsgqgrlml
yygnssafpltnfnvtlnsiagltlqpqsiapviqpkaqlqqpvtfsctseftespviti
nfltpgkpititlrlpiviskffeplrlssgdffarwktisgkpleiqeifkstkpidiq
synrviqeglnitvlkqvdpnpnnivasclfpfgsngqpinxyirietnpqanmcrlti

Frame B:
ni*kdx*k*iinxlikkxx*kkkkvxxkvkxvixxxxgqi*xx---

---xiklxnfkwwx*snan*dfsiiqeimfs**gvysmrivcyklv*nqninqvkvd*cy
tmeivqhsh*pismlpliqslv*hynhnrlhqlfnqrhnynnqslfhvhqsllnhqlspl
islhqvnqslspfvfqllsvnslnhyvyqvvtfslvgrqslvnhwkfkkslnqlnqsifn
htiesfkkv*ilpysnr*iqiqiil*hlvsshsvqmvnq*txilelkpthkqicvvlll

Frame C:
ifkkxdknk*lix**kkxxkkkkr*xkr*xx*fxxxkakfxxx---

---ASSSPISSGGXDPMQIKILASYKRLCLVSEGCTL*g*yvtswfeirisirsr*invi
lwk*fsipidqfqcyp*fnrwfnitttidctsystkgtitttshffmyirvy*itsyhh*
fpytr*tnhyhpsssncyq*il*titfikw*lfrslednlw*tignsrnl*in*tnrysi
iq*shsrrfkyyrtqtgrsksk*ycsilslpirfkwstnklly*n*nqptskyvssyy*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA155 (VHA155Q) /CSM/VH/VHA1-C/VHA155Q.Seq.d/                       1380   0.0
VHP865 (VHP865Q) /CSM/VH/VHP8-C/VHP865Q.Seq.d/                       1364   0.0
CHR358 (CHR358Q) /CSM/CH/CHR3-C/CHR358Q.Seq.d/                       1364   0.0
CHL833 (CHL833Q) /CSM/CH/CHL8-B/CHL833Q.Seq.d/                       1364   0.0
AHM450 (AHM450Q) /CSM/AH/AHM4-C/AHM450Q.Seq.d/                       1364   0.0
VHJ566 (VHJ566Q) /CSM/VH/VHJ5-C/VHJ566Q.Seq.d/                       1358   0.0
CHM383 (CHM383Q) /CSM/CH/CHM3-D/CHM383Q.Seq.d/                       1358   0.0
CHL210 (CHL210Q) /CSM/CH/CHL2-A/CHL210Q.Seq.d/                       1350   0.0
AFF737 (AFF737Q) /CSM/AF/AFF7-B/AFF737Q.Seq.d/                       1350   0.0
AHM744 (AHM744Q) /CSM/AH/AHM7-B/AHM744Q.Seq.d/                       1336   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.  1219   0.0    2
EK364072|EK364072.1 1095469390160 Global-Ocean-Sampling_GS-31-01-01-1P3-1P8KB marine metagenome genomic clone 1061005864011 3', genomic survey sequence.    46   1.6    1
DQ767612|DQ767612.1 Triticum aestivum clone BAC TA1057C05, *** SEQUENCING IN PROGRESS ***, 14 unordered pieces.    32   3.1    2
DX770216|DX770216.1 2689995 VV07 Ustilago maydis genomic clone 1204168, genomic survey sequence.    44   6.2    1
DX756422|DX756422.1 2365937 VV03 Ustilago maydis genomic clone 1025158, genomic survey sequence.    44   6.2    1
DX733440|DX733440.1 2315736 VV03 Ustilago maydis genomic clone 1006812, genomic survey sequence.    44   6.2    1
DX722040|DX722040.1 2310775 VV03 Ustilago maydis genomic clone 1002065, genomic survey sequence.    44   6.2    1
DX660090|DX660090.1 2021272 VV02 Ustilago maydis genomic clone 817908, genomic survey sequence.    44   6.2    1
DX392359|DX392359.1 GE__Sa0078E03.b1 Gossypium exiguum WGS library Gossypium exiguum genomic similar to Gossypium exiguum repetitive sequence of unknown type, genomic survey sequence.    44   6.2    1
U88590|U88590.2 Lacerta portschinskii ATP synthase subunit 6 gene, partial cds; mitochondrial gene for mitochondrial product.    44   6.2    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86KI1) RecName: Full=AP-2 complex subunit alpha-2; AltName: Fu...   408   e-120
AE014134_101(AE014134|pid:none) Drosophila melanogaster chromoso...   161   3e-38
(P91926) RecName: Full=AP-2 complex subunit alpha; AltName: Full...   161   3e-38
(Q29N38) RecName: Full=AP-2 complex subunit alpha; AltName: Full...   159   1e-37
AK293763_1(AK293763|pid:none) Homo sapiens cDNA FLJ57955 complet...   151   2e-35
AK223558_1(AK223558|pid:none) Homo sapiens mRNA for adaptor-rela...   151   2e-35
AK088500_1(AK088500|pid:none) Mus musculus 2 days neonate thymus...   151   3e-35
PDBN(1QTS)A MOL_ID: 1;MOL_ID: 1; MOLECULE: AP-2 CLATHRIN ADAPTOR...   151   3e-35
PDBN(1B9K)A MOL_ID: 1;MOL_ID: 1; MOLECULE: ALPHA-ADAPTIN APPENDA...   150   4e-35
CR857879_1(CR857879|pid:none) Pongo abelii mRNA; cDNA DKFZp459P0...   150   4e-35

 psg: 0.41  gvh: 0.49  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.16  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHA155 is nuc

>VHA155F.Seq
TAATATTTAAAAAGATNGATAAAAATAAATAATTAATCANTTAATAAAAAAAAANTANTA
AAAAAAAAAAAAGGTAAANNAAAAGGTAAAANNNGTAATTTANNANTANNAAGGCCAAAT
TTAANNANNAAANNNNNNNNNN

>VHA155Z.Seq
NNNNNNNNNNANGCATCAAGCTCNCCAATTTCAAGTGGTGGNTNTGATCCAATGCAAATT
AAGATTTTAGCATCATACAAGAGATTATGTTTAGTTAGTGAGGGGTGTACTCTATGAGGA
TAGTATGTTACAAGTTGGTTTGAAATCAGAATATCAATCAGGTCAAGGTAGATTAATGTT
ATACTATGGAAATAGTTCAGCATTCCCATTGACCAATTTCAATGTTACCCTTAATTCAAT
CGCTGGTTTAACATTACAACCACAATCGATTGCACCAGTTATTCAACCAAAGGCACAATT
ACAACAACCAGTCACTTTTTCATGTACATCAGAGTTTACTGAATCACCAGTTATCACCAT
TAATTTCCTTACACCAGGTAAACCAATCACTATCACCCTTCGTCTTCCAATTGTTATCAG
TAAATTCTTTGAACCATTACGTTTATCAAGTGGTGACTTTTTCGCTCGTTGGAAGACAAT
CTCTGGTAAACCATTGGAAATTCAAGAAATCTTTAAATCAACTAAACCAATCGATATTCA
ATCATACAATAGAGTCATTCAAGAAGGTTTAAATATTACCGTACTCAAACAGGTAGATCC
AAATCCAAATAATATTGTAGCATCTTGTCTCTTCCCATTCGGTTCAAATGGTCAACCAAT
AAACTNTTATATTAGAATTGAAACCAACCCACAAGCAAATATGTGTCGTCTTACTATTAG

>VHA155P.Seq
TAATATTTAAAAAGATNGATAAAAATAAATAATTAATCANTTAATAAAAAAAAANTANTA
AAAAAAAAAAAAGGTAAANNAAAAGGTAAAANNNGTAATTTANNANTANNAAGGCCAAAT
TTAANNANNAAA----------ANGCATCAAGCTCNCCAATTTCAAGTGGTGGNTNTGAT
CCAATGCAAATTAAGATTTTAGCATCATACAAGAGATTATGTTTAGTTAGTGAGGGGTGT
ACTCTATGAGGATAGTATGTTACAAGTTGGTTTGAAATCAGAATATCAATCAGGTCAAGG
TAGATTAATGTTATACTATGGAAATAGTTCAGCATTCCCATTGACCAATTTCAATGTTAC
CCTTAATTCAATCGCTGGTTTAACATTACAACCACAATCGATTGCACCAGTTATTCAACC
AAAGGCACAATTACAACAACCAGTCACTTTTTCATGTACATCAGAGTTTACTGAATCACC
AGTTATCACCATTAATTTCCTTACACCAGGTAAACCAATCACTATCACCCTTCGTCTTCC
AATTGTTATCAGTAAATTCTTTGAACCATTACGTTTATCAAGTGGTGACTTTTTCGCTCG
TTGGAAGACAATCTCTGGTAAACCATTGGAAATTCAAGAAATCTTTAAATCAACTAAACC
AATCGATATTCAATCATACAATAGAGTCATTCAAGAAGGTTTAAATATTACCGTACTCAA
ACAGGTAGATCCAAATCCAAATAATATTGTAGCATCTTGTCTCTTCCCATTCGGTTCAAA
TGGTCAACCAATAAACTNTTATATTAGAATTGAAACCAACCCACAAGCAAATATGTGTCG
TCTTACTATTAG