>VHA131 (VHA131Q) /CSM/VH/VHA1-B/VHA131Q.Seq.d/
ATTTGTATATTCACAACCATCAACTTTAACAATTCAAGGAACAATTTTTGATCAACATCC
AGATTATAATAACAATTTCGAACCAAAGGGAGGATCCATTAAAAAAAATTTAGTCTTAAA
AACTCTTGGAAGTAGCAAAGTACCAACTCTTGTTTCTTTAGATCCAGAGGATGATACAAA
TGAAGATGGTAGAATGATTACACCAAGCTTGTTCCAATACTTTTATCAAACATCATCAAA
ACCATTAACCAAAAATTCTGGTGCAAATGTCCCAATTCCAATTAACCTTGTCTTGAATTA
CGATTCCAAAAAACAAGTTTATGTTTACAATGATCAATATTTTTTCCCAATTGATTACCA
AGGATTTGATGTTGATTCAAAATATAGAACTTATAATAATGGTTCAGGATATCATAATTT
CCATTTTTGTTTAAAATTGAATACTAAATTCACCTATCAAGGTATTGAAGATTTTTACTT
TATTGGTGATGATGATGTTTGGGTATTCATTAATAACAAATTGGTTGTCGATTTAGGTGG
TTTACATAGTAGAGAXXXXXXXXXXTTTTCCACACACCAATTAACAAATGTTCAGGTTGT
ATGACTTGTAATCCAGTTTCTGGTAATTGTGATTTATCCGACACCGTTTGCCAAGATGGT
AATGAATGTACTACCAATGTTTGTGAATCAAGTGATAGTGTCCTTGGTGTATGTACAAAC
TCAACAGTTGAATGCGGTGCTACCAACACTGATAAATGTATCCAATTCTCTTGTAATGAA
GCCACCGGTTGCCAACAATCAGCTGTCGTTTGTCCAGATGTTGGTAGTTGTTTAGTTGGT
TATTGTGATAGTAAACAAGGTTGTCTCACCAAACCAAGATCTTGCGATACTGGTGTTTTC
TGTTTAACTGGTGAATGTATTGAAAATGCTGGTGGTTGTATCGTCTATGAAAAACGTTGT
GATGCTGATAATGGAAGATGTCAACAAGGTGTATGTGTAAATAATACCGCCACTGAAGAA
GGTTATTGTAAATCTGAAGACTATGATCCATTACCATTCATTTGTAAAACTGGTGCTGTC
GTTTCAACTGCTGTCATTGCTGGTGTCACTGTCGCTGGTGCTGTTGCACTTGGTGTTTTC
ATTTATGGTGGTAAAAGAGGTTATGACTATTGGGAAGAATCAAGAAATGTTCAATTCAGT
GGTTCAAATTCTAATCCACTTTATGAACAAAATCCAAATGGTTCTGGTGTAAATCCATTA
TATAATGATAATTACTGACATTATAAAATAAANTTATGTACNTATATAAAT

FVYSQPSTLTIQGTIFDQHPDYNNNFEPKGGSIKKNLVLKTLGSSKVPTLVSLDPEDDTN
EDGRMITPSLFQYFYQTSSKPLTKNSGANVPIPINLVLNYDSKKQVYVYNDQYFFPIDYQ
GFDVDSKYRTYNNGSGYHNFHFCLKLNTKFTYQGIEDFYFIGDDDVWVFINNKLVVDLGG
LHSR---

---FHTPINKCSGCMTCNPVSGNCDLSDTVCQDGNECTTNVCESSDSVLGVCTNSTVECG
ATNTDKCIQFSCNEATGCQQSAVVCPDVGSCLVGYCDSKQGCLTKPRSCDTGVFCLTGEC
IENAGGCIVYEKRCDADNGRCQQGVCVNNTATEEGYCKSEDYDPLPFICKTGAVVSTAVI
AGVTVAGAVALGVFIYGGKRGYDYWEESRNVQFSGSNSNPLYEQNPNGSGVNPLYNDNY*
hykixlctyin

Frame A:
icifttinfnnsrnnf*stsrl**qfrtkgrih*kkfslknswk*qstnscffrsrg*yk
*rw*ndytklvpillsniiktinqkfwckcpnsn*pclelrfqktslclq*siffpn*lp
ri*c*fki*nl**wfris*fpflfkiey*ihlsry*rfllyw***clgih**qigcrfrw
ft**r---

---fsthqltnvqvv*lviqflviviyptpfakmvmnvlpmfvnqvivslvyvqtqqlna
vlptlinvsnslvmkppvannqlsfvqmlvvv*lvivivnkvvspnqdlailvfsv*lvn
vlkmlvvvssmknvvmlimedvnkvyv*iipplkkvivnlktmihyhsfvklvlsfqlls
llvslslvllhlvfsfmvvkevmtigknqemfnsvvqilihfmnkiqmvlv*ihyimiit
diik*xyvxi*

Frame B:
FVYSQPSTLTIQGTIFDQHPDYNNNFEPKGGSIKKNLVLKTLGSSKVPTLVSLDPEDDTN
EDGRMITPSLFQYFYQTSSKPLTKNSGANVPIPINLVLNYDSKKQVYVYNDQYFFPIDYQ
GFDVDSKYRTYNNGSGYHNFHFCLKLNTKFTYQGIEDFYFIGDDDVWVFINNKLVVDLGG
LHSR---

---fphtn*qmfrlydl*ssfw*l*firhrlprw**myyqcl*ik**cpwcmyklns*mr
cyqh**mypill**shrlptiscrlsrcw*lfswll***trlshqtkilrywcflfnw*m
y*kcwwlyrl*ktl*c**wkmstrcmck*yrh*rrll*i*rl*sitihl*nwccrfncch
cwchcrwccctwcfhlww*krl*llgrikkcsiqwfkf*stl*tkskwfwcksii***ll
tl*nkxmyxyk

Frame C:
lyihnhql*qfkeqfliniqiiitisnqredplkki*s*kllevakyqllfl*iqrmiqm
kmve*lhqacsntfikhhqnh*pkilvqmsqfqltls*itipknkfmftminifsqlitk
dlmliqnieliimvqdiiisifv*n*ilnspikvlkiftllvmmmfgyslitnwlsi*vv
yive---

---FHTPINKCSGCMTCNPVSGNCDLSDTVCQDGNECTTNVCESSDSVLGVCTNSTVECG
ATNTDKCIQFSCNEATGCQQSAVVCPDVGSCLVGYCDSKQGCLTKPRSCDTGVFCLTGEC
IENAGGCIVYEKRCDADNGRCQQGVCVNNTATEEGYCKSEDYDPLPFICKTGAVVSTAVI
AGVTVAGAVALGVFIYGGKRGYDYWEESRNVQFSGSNSNPLYEQNPNGSGVNPLYNDNY*
hykixlctyin

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA131 (VHA131Q) /CSM/VH/VHA1-B/VHA131Q.Seq.d/                       2480   0.0
VHB544 (VHB544Q) /CSM/VH/VHB5-B/VHB544Q.Seq.d/                       1409   0.0
VFG436 (VFG436Q) /CSM/VF/VFG4-B/VFG436Q.Seq.d/                       1409   0.0
VHI823 (VHI823Q) /CSM/VH/VHI8-A/VHI823Q.Seq.d/                       1403   0.0
VHJ217 (VHJ217Q) /CSM/VH/VHJ2-A/VHJ217Q.Seq.d/                       1398   0.0
VHP731 (VHP731Q) /CSM/VH/VHP7-B/VHP731Q.Seq.d/                       1396   0.0
VFM306 (VFM306Q) /CSM/VF/VFM3-A/VFM306Q.Seq.d/                       1396   0.0
VHO544 (VHO544Q) /CSM/VH/VHO5-B/VHO544Q.Seq.d/                       1390   0.0
VHA180 (VHA180Q) /CSM/VH/VHA1-D/VHA180Q.Seq.d/                       1390   0.0
VFO330 (VFO330Q) /CSM/VF/VFO3-B/VFO330Q.Seq.d/                       1390   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U67940|U67940.1 Dictyostelium discoideum ORFveg106 mRNA, complete cds.  1396   0.0    2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   113   2e-23  11
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    70   2e-10  5
AB053203|AB053203.1 Dictyostelium discoideum psiA mRNA for Psi facor, complete cds.    52   4e-07  4
AJ548837|AJ548837.1 Dictyostelium discoideum mRNA for extracellular signalling molecule  DicA (dicA gene).    46   0.017  3
AJ548838|AJ548838.1 Dictyostelium discoideum dicA gene for extracellular signalling  molecule DicA, exons 1-2.    46   0.024  3
EJ381894|EJ381894.1 1092963764900 Global-Ocean-Sampling_GS-28-01-01-1P6-2P0KB marine metagenome genomic clone 1061002345598 5', genomic survey sequence.    50   0.15   1
AE014308|AE014308.1 Homo sapiens chromosome 13q34 schizophrenia region contig 1 section 5 of 11 of the complete sequence.    42   0.35   6
H71387|H71387.1 yu55f03.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:230045 5', mRNA sequence.    48   0.61   1
AC153032|AC153032.2 Bos taurus clone CH240-22E7, WORKING DRAFT SEQUENCE, 15 unordered pieces.    48   0.61   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   461   e-128
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   229   2e-58
(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                223   9e-57
(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                194   4e-48
(Q55FQ4) RecName: Full=Protein psiB; Flags: Precursor;                185   4e-45
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   184   8e-45
(Q968Z6) RecName: Full=Protein psiA; AltName: Full=Prespore-cell...   168   5e-40
(Q55FQ5) RecName: Full=Protein psiS; Flags: Precursor;                164   9e-39
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                123   3e-33
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                139   2e-31

 psg: 0.88  gvh: 0.44  alm: 0.22  top: 0.60  tms: 0.07  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHA131 is cyt

>VHA131F.Seq
ATTTGTATATTCACAACCATCAACTTTAACAATTCAAGGAACAATTTTTGATCAACATCC
AGATTATAATAACAATTTCGAACCAAAGGGAGGATCCATTAAAAAAAATTTAGTCTTAAA
AACTCTTGGAAGTAGCAAAGTACCAACTCTTGTTTCTTTAGATCCAGAGGATGATACAAA
TGAAGATGGTAGAATGATTACACCAAGCTTGTTCCAATACTTTTATCAAACATCATCAAA
ACCATTAACCAAAAATTCTGGTGCAAATGTCCCAATTCCAATTAACCTTGTCTTGAATTA
CGATTCCAAAAAACAAGTTTATGTTTACAATGATCAATATTTTTTCCCAATTGATTACCA
AGGATTTGATGTTGATTCAAAATATAGAACTTATAATAATGGTTCAGGATATCATAATTT
CCATTTTTGTTTAAAATTGAATACTAAATTCACCTATCAAGGTATTGAAGATTTTTACTT
TATTGGTGATGATGATGTTTGGGTATTCATTAATAACAAATTGGTTGTCGATTTAGGTGG
TTTACATAGTAGAGANNNNNNNNNN

>VHA131Z.Seq
NNNNNNNNNNTTTTCCACACACCAATTAACAAATGTTCAGGTTGTATGACTTGTAATCCA
GTTTCTGGTAATTGTGATTTATCCGACACCGTTTGCCAAGATGGTAATGAATGTACTACC
AATGTTTGTGAATCAAGTGATAGTGTCCTTGGTGTATGTACAAACTCAACAGTTGAATGC
GGTGCTACCAACACTGATAAATGTATCCAATTCTCTTGTAATGAAGCCACCGGTTGCCAA
CAATCAGCTGTCGTTTGTCCAGATGTTGGTAGTTGTTTAGTTGGTTATTGTGATAGTAAA
CAAGGTTGTCTCACCAAACCAAGATCTTGCGATACTGGTGTTTTCTGTTTAACTGGTGAA
TGTATTGAAAATGCTGGTGGTTGTATCGTCTATGAAAAACGTTGTGATGCTGATAATGGA
AGATGTCAACAAGGTGTATGTGTAAATAATACCGCCACTGAAGAAGGTTATTGTAAATCT
GAAGACTATGATCCATTACCATTCATTTGTAAAACTGGTGCTGTCGTTTCAACTGCTGTC
ATTGCTGGTGTCACTGTCGCTGGTGCTGTTGCACTTGGTGTTTTCATTTATGGTGGTAAA
AGAGGTTATGACTATTGGGAAGAATCAAGAAATGTTCAATTCAGTGGTTCAAATTCTAAT
CCACTTTATGAACAAAATCCAAATGGTTCTGGTGTAAATCCATTATATAATGATAATTAC
TGACATTATAAAATAAANTTATGTACNTATATAAAT

>VHA131P.Seq
ATTTGTATATTCACAACCATCAACTTTAACAATTCAAGGAACAATTTTTGATCAACATCC
AGATTATAATAACAATTTCGAACCAAAGGGAGGATCCATTAAAAAAAATTTAGTCTTAAA
AACTCTTGGAAGTAGCAAAGTACCAACTCTTGTTTCTTTAGATCCAGAGGATGATACAAA
TGAAGATGGTAGAATGATTACACCAAGCTTGTTCCAATACTTTTATCAAACATCATCAAA
ACCATTAACCAAAAATTCTGGTGCAAATGTCCCAATTCCAATTAACCTTGTCTTGAATTA
CGATTCCAAAAAACAAGTTTATGTTTACAATGATCAATATTTTTTCCCAATTGATTACCA
AGGATTTGATGTTGATTCAAAATATAGAACTTATAATAATGGTTCAGGATATCATAATTT
CCATTTTTGTTTAAAATTGAATACTAAATTCACCTATCAAGGTATTGAAGATTTTTACTT
TATTGGTGATGATGATGTTTGGGTATTCATTAATAACAAATTGGTTGTCGATTTAGGTGG
TTTACATAGTAGAGA----------TTTTCCACACACCAATTAACAAATGTTCAGGTTGT
ATGACTTGTAATCCAGTTTCTGGTAATTGTGATTTATCCGACACCGTTTGCCAAGATGGT
AATGAATGTACTACCAATGTTTGTGAATCAAGTGATAGTGTCCTTGGTGTATGTACAAAC
TCAACAGTTGAATGCGGTGCTACCAACACTGATAAATGTATCCAATTCTCTTGTAATGAA
GCCACCGGTTGCCAACAATCAGCTGTCGTTTGTCCAGATGTTGGTAGTTGTTTAGTTGGT
TATTGTGATAGTAAACAAGGTTGTCTCACCAAACCAAGATCTTGCGATACTGGTGTTTTC
TGTTTAACTGGTGAATGTATTGAAAATGCTGGTGGTTGTATCGTCTATGAAAAACGTTGT
GATGCTGATAATGGAAGATGTCAACAAGGTGTATGTGTAAATAATACCGCCACTGAAGAA
GGTTATTGTAAATCTGAAGACTATGATCCATTACCATTCATTTGTAAAACTGGTGCTGTC
GTTTCAACTGCTGTCATTGCTGGTGTCACTGTCGCTGGTGCTGTTGCACTTGGTGTTTTC
ATTTATGGTGGTAAAAGAGGTTATGACTATTGGGAAGAATCAAGAAATGTTCAATTCAGT
GGTTCAAATTCTAATCCACTTTATGAACAAAATCCAAATGGTTCTGGTGTAAATCCATTA
TATAATGATAATTACTGACATTATAAAATAAANTTATGTACNTATATAAAT