>VHA130 (VHA130Q) /CSM/VH/VHA1-B/VHA130Q.Seq.d/
AATATTTTTTGATTATTCATATTCATATATATAGAATTTCAATAGGATACGAATATGAGT
AGTGGAATATCATTTGCAAGAAATCTACATATTAGAATTAAAAATGGTGTTACATCAGCA
CCATTTGGTTATGCATCAGTATTGAAATTTGGTATACCAGTGATACCACCAAGAACTCCA
GGTAAAATTGAAGTTATTGAATCACCAATTTTAAAATATGCACAAGAATTTGCTGATAGT
CATCGTGAAATTGGCTATTTAACAGATAATCGTTTATCACAAGATGCATACAATTATGCA
AGTTTAAAACTTTCATACATGAAAAAAGGTTTAAGTGAATCACAAGCTAATACACAAGCA
AAAAAAGATGTTATAAAAAATTTAGAAGTTCGTTCATTAGAGTTGGATCTCATTCAAAAT
CAAGCTCTTAAAATTGGTATTCCATCTTTTCAACCAAAATCTCATTTAGAACAAATTAAT
ACTTTAACAAAAAAAGTTGAAGATCGTAGAGCAGAATATGAAATTATTGAAGCAAGAGAA
AAATTAGAAGGTACATTGGCACTTAAATTTCAATATGGTGATATGACACCATTAACATTA
TCAAATGTATCATTATTATCACCAATTCAATATTTTATTTTTGTTXXXXXXXXXXACAGA
TATTGAAATGGAAATTGTGGAAATCTTGGAAAGTTTAACAAATCAATCATTGACCGCCAG
AAAGAATGCTCATAATCATCTATACCCAGAGTATAAGAATGCCTCAAAGTCAATCATCAA
TGAAAAGAAGATCTCTGAAGATTTAATTAGATATGTTATCGATTCTGATCTCATCTCTCA
TAAATATAATGTTGCCCCATCCATCGATAAAGAAGACAAGGTCGATTTTAGAACTTTGAA
ATTGGATCCAGAAACTGAGCATCAATATGCCCACCACATTAAAGAGTTTAGAGATAAAGA
TGCCAAAGGTCTCATCTCTCAACAAGATGCCTTTGAATTTACAAATTACATCAACTACTT
AAAGGAAAGTTCTGCTACTTCTCCAATCATTCAATCTCTTAAATCTTCAATTCATATGAA
ATCAAATAACAATGAACTCATCGATCAATTCAATGCTGAATCTTTAGAAAAAGATACCAC
CACCACCGATAAACTCTCCCCTGAAGGTTTGTTCTTTAAGCAACTCGGAGAAATGTTCCC
TTCCGAAGCTGATAAAGAAATTGCTATCAATACTCTCTTATATCAAAATAATAAAAATCA
AAAGATTCAAAAACTTGAAAATGATTTGGTTAATGCAATCTCTAAGGATTCCACCATTCG
TATTCCTTNTCCAAAAGAA

nif*lfifiyiefq*DTNMSSGISFARNLHIRIKNGVTSAPFGYASVLKFGIPVIPPRTP
GKIEVIESPILKYAQEFADSHREIGYLTDNRLSQDAYNYASLKLSYMKKGLSESQANTQA
KKDVIKNLEVRSLELDLIQNQALKIGIPSFQPKSHLEQINTLTKKVEDRRAEYEIIEARE
KLEGTLALKFQYGDMTPLTLSNVSLLSPIQYFIFV---

---TDIEMEIVEILESLTNQSLTARKNAHNHLYPEYKNASKSIINEKKISEDLIRYVIDS
DLISHKYNVAPSIDKEDKVDFRTLKLDPETEHQYAHHIKEFRDKDAKGLISQQDAFEFTN
YINYLKESSATSPIIQSLKSSIHMKSNNNELIDQFNAESLEKDTTTTDKLSPEGLFFKQL
GEMFPSEADKEIAINTLLYQNNKNQKIQKLENDLVNAISKDSTIRIPXPKE

Frame A:
nif*lfifiyiefq*DTNMSSGISFARNLHIRIKNGVTSAPFGYASVLKFGIPVIPPRTP
GKIEVIESPILKYAQEFADSHREIGYLTDNRLSQDAYNYASLKLSYMKKGLSESQANTQA
KKDVIKNLEVRSLELDLIQNQALKIGIPSFQPKSHLEQINTLTKKVEDRRAEYEIIEARE
KLEGTLALKFQYGDMTPLTLSNVSLLSPIQYFIFV---

---TDIEMEIVEILESLTNQSLTARKNAHNHLYPEYKNASKSIINEKKISEDLIRYVIDS
DLISHKYNVAPSIDKEDKVDFRTLKLDPETEHQYAHHIKEFRDKDAKGLISQQDAFEFTN
YINYLKESSATSPIIQSLKSSIHMKSNNNELIDQFNAESLEKDTTTTDKLSPEGLFFKQL
GEMFPSEADKEIAINTLLYQNNKNQKIQKLENDLVNAISKDSTIRIPXPKE

Frame B:
iffdysysyi*nfnriri*vveyhlqeiyilelkmvlhqhhlvmhqy*nlvyq*yhqelq
vklkllnhqf*nmhknllivivklai*qiivyhkmhtimqv*nfht*kkv*vnhklihkq
kkml*ki*kfvh*swisfkikllklvfhlfnqnli*nklil*qkklkiveqnmkllkqek
n*kvhwhlnfnmvi*hh*hyqmyhyyhqfnilfl---

---qilkwklwkswkv*qinh*ppermliiiytqsirmpqsqssmkrrslki*ldmlsil
isslinimlphpsikktrsilel*nwiqklsinmpttlksleikmpkvsslnkmplnlqi
tstt*rkvllllqsfnllnlqfi*nqitmnssinsmlnl*kkippppinsplkvcslsns
ekcslpklikkllsilsyikiikikrfknlkmiwlmqslrippfvflxqk

Frame C:
yfliihihiyrisigyeye*wniickksty*n*kwcyistiwlcisieiwytsdttknsr
*n*sy*itnfkictric**ss*nwlfnr*sfitrciqlckfktfihekrfk*its*ytsk
krcykkfrssfirvgshsksss*nwysifstkisfrtn*yfnkks*rs*sri*ny*skrk
irryigt*isiw*ydtiniikciiiitnsifyfc---

---ry*ngncgnlgkfnksiidrqkecs*ssiprv*eclkvnhq*kedl*rfn*icyrf*
shls*i*ccpihr*rrqgrf*nfeigsrn*asicpph*rv*r*rcqrshlstrcl*iykl
hqllkgkfcyfsnhsis*ifnsyeik*q*thrsiqc*ifrkryhhhr*tlp*rfvl*atr
rnvpfrs**rncyqyslisk**kskdskt*k*fg*cnl*gfhhsysxskr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA130 (VHA130Q) /CSM/VH/VHA1-B/VHA130Q.Seq.d/                       2317   0.0
VHG269 (VHG269Q) /CSM/VH/VHG2-C/VHG269Q.Seq.d/                       1195   0.0
VHM828 (VHM828Q) /CSM/VH/VHM8-B/VHM828Q.Seq.d/                       1178   0.0
SHK605 (SHK605Q) /CSM/SH/SHK6-A/SHK605Q.Seq.d/                       1178   0.0
VHN206 (VHN206Q) /CSM/VH/VHN2-A/VHN206Q.Seq.d/                       1162   0.0
VHA418 (VHA418Q) /CSM/VH/VHA4-A/VHA418Q.Seq.d/                       1162   0.0
VHN232 (VHN232Q) /CSM/VH/VHN2-B/VHN232Q.Seq.d/                       1144   0.0
AHL671 (AHL671Q) /CSM/AH/AHL6-C/AHL671Q.Seq.d/                       1098   0.0
VHC224 (VHC224Q) /CSM/VH/VHC2-A/VHC224Q.Seq.d/                       1096   0.0
VHO315 (VHO315Q) /CSM/VH/VHO3-A/VHO315Q.Seq.d/                       1092   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

EJ232326|EJ232326.1 1092404030385 Global-Ocean-Sampling_GS-27-01-01-1P8-2P0KB marine metagenome genomic clone 1061001533792 5', genomic survey sequence.    44   0.004  2
AC167710|AC167710.1 Medicago truncatula chromosome 7 clone mth2-13n1, *** SEQUENCING IN PROGRESS ***, 5 ordered pieces.    36   0.65   7
AE014851|AE014851.1 Plasmodium falciparum 3D7 chromosome 12 section 8 of 9 of the complete sequence.    32   2.0    10
AL627386|AL627386.8 Mouse DNA sequence from clone RP23-278A10 on chromosome X.    38   2.2    6
DU934199|DU934199.1 360919 Tomato EcoRI BAC Library Solanum lycopersicum genomic clone SL_EcoRI0074I21 5, genomic survey sequence.    46   2.5    1
AM433708|AM433708.1 Vitis vinifera, whole genome shotgun sequence, contig VV78X218793.1, clone ENTAV 115.    46   2.5    1
DQ481665|DQ481665.1 Takifugu rubripes HoxBa gene cluster, complete sequence.    46   2.5    1
AY277967|AY277967.1 Takifugu rubripes transcription factor Sox10b (Sox10b) gene, complete cds.    46   2.5    1
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    32   2.6    12
AC143329|AC143329.3 Mus musculus BAC clone RP23-347I6 from chromosome 7, complete sequence.    44   2.9    4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF202180_1(AF202180|pid:none) Plasmodium falciparum erythrocyte ...    47   0.001
AE014186_473(AE014186|pid:none) Plasmodium falciparum 3D7 chromo...    47   0.001
CU179680_105(CU179680|pid:none) Mycoplasma agalactiae PG2 chromo...    46   0.003
BT044549_1(BT044549|pid:none) Drosophila melanogaster SD15969 fu...    40   0.19
T13829(T13829) Tpr homolog - fruit fly (Drosophila melanogaster)...    40   0.19
AF130119_2(AF130119|pid:none) Mycoplasma bovis oligopeptide perm...    40   0.19
CP001130_776(CP001130|pid:none) Hydrogenobaculum sp. Y04AAS1, co...    39   0.42
CP000764_1309(CP000764|pid:none) Bacillus cereus subsp. cytotoxi...    38   0.72
CP000383_40(CP000383|pid:none) Cytophaga hutchinsonii ATCC 33406...    38   0.72
AE001362_41(AE001362|pid:none) Plasmodium falciparum 3D7 chromos...    37   1.2

 psg: 0.75  gvh: 0.40  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHA130 is nuc

>VHA130F.Seq
AATATTTTTTGATTATTCATATTCATATATATAGAATTTCAATAGGATACGAATATGAGT
AGTGGAATATCATTTGCAAGAAATCTACATATTAGAATTAAAAATGGTGTTACATCAGCA
CCATTTGGTTATGCATCAGTATTGAAATTTGGTATACCAGTGATACCACCAAGAACTCCA
GGTAAAATTGAAGTTATTGAATCACCAATTTTAAAATATGCACAAGAATTTGCTGATAGT
CATCGTGAAATTGGCTATTTAACAGATAATCGTTTATCACAAGATGCATACAATTATGCA
AGTTTAAAACTTTCATACATGAAAAAAGGTTTAAGTGAATCACAAGCTAATACACAAGCA
AAAAAAGATGTTATAAAAAATTTAGAAGTTCGTTCATTAGAGTTGGATCTCATTCAAAAT
CAAGCTCTTAAAATTGGTATTCCATCTTTTCAACCAAAATCTCATTTAGAACAAATTAAT
ACTTTAACAAAAAAAGTTGAAGATCGTAGAGCAGAATATGAAATTATTGAAGCAAGAGAA
AAATTAGAAGGTACATTGGCACTTAAATTTCAATATGGTGATATGACACCATTAACATTA
TCAAATGTATCATTATTATCACCAATTCAATATTTTATTTTTGTTNNNNNNNNNN

>VHA130Z.Seq
NNNNNNNNNNACAGATATTGAAATGGAAATTGTGGAAATCTTGGAAAGTTTAACAAATCA
ATCATTGACCGCCAGAAAGAATGCTCATAATCATCTATACCCAGAGTATAAGAATGCCTC
AAAGTCAATCATCAATGAAAAGAAGATCTCTGAAGATTTAATTAGATATGTTATCGATTC
TGATCTCATCTCTCATAAATATAATGTTGCCCCATCCATCGATAAAGAAGACAAGGTCGA
TTTTAGAACTTTGAAATTGGATCCAGAAACTGAGCATCAATATGCCCACCACATTAAAGA
GTTTAGAGATAAAGATGCCAAAGGTCTCATCTCTCAACAAGATGCCTTTGAATTTACAAA
TTACATCAACTACTTAAAGGAAAGTTCTGCTACTTCTCCAATCATTCAATCTCTTAAATC
TTCAATTCATATGAAATCAAATAACAATGAACTCATCGATCAATTCAATGCTGAATCTTT
AGAAAAAGATACCACCACCACCGATAAACTCTCCCCTGAAGGTTTGTTCTTTAAGCAACT
CGGAGAAATGTTCCCTTCCGAAGCTGATAAAGAAATTGCTATCAATACTCTCTTATATCA
AAATAATAAAAATCAAAAGATTCAAAAACTTGAAAATGATTTGGTTAATGCAATCTCTAA
GGATTCCACCATTCGTATTCCTTNTCCAAAAGAA

>VHA130P.Seq
AATATTTTTTGATTATTCATATTCATATATATAGAATTTCAATAGGATACGAATATGAGT
AGTGGAATATCATTTGCAAGAAATCTACATATTAGAATTAAAAATGGTGTTACATCAGCA
CCATTTGGTTATGCATCAGTATTGAAATTTGGTATACCAGTGATACCACCAAGAACTCCA
GGTAAAATTGAAGTTATTGAATCACCAATTTTAAAATATGCACAAGAATTTGCTGATAGT
CATCGTGAAATTGGCTATTTAACAGATAATCGTTTATCACAAGATGCATACAATTATGCA
AGTTTAAAACTTTCATACATGAAAAAAGGTTTAAGTGAATCACAAGCTAATACACAAGCA
AAAAAAGATGTTATAAAAAATTTAGAAGTTCGTTCATTAGAGTTGGATCTCATTCAAAAT
CAAGCTCTTAAAATTGGTATTCCATCTTTTCAACCAAAATCTCATTTAGAACAAATTAAT
ACTTTAACAAAAAAAGTTGAAGATCGTAGAGCAGAATATGAAATTATTGAAGCAAGAGAA
AAATTAGAAGGTACATTGGCACTTAAATTTCAATATGGTGATATGACACCATTAACATTA
TCAAATGTATCATTATTATCACCAATTCAATATTTTATTTTTGTT----------ACAGA
TATTGAAATGGAAATTGTGGAAATCTTGGAAAGTTTAACAAATCAATCATTGACCGCCAG
AAAGAATGCTCATAATCATCTATACCCAGAGTATAAGAATGCCTCAAAGTCAATCATCAA
TGAAAAGAAGATCTCTGAAGATTTAATTAGATATGTTATCGATTCTGATCTCATCTCTCA
TAAATATAATGTTGCCCCATCCATCGATAAAGAAGACAAGGTCGATTTTAGAACTTTGAA
ATTGGATCCAGAAACTGAGCATCAATATGCCCACCACATTAAAGAGTTTAGAGATAAAGA
TGCCAAAGGTCTCATCTCTCAACAAGATGCCTTTGAATTTACAAATTACATCAACTACTT
AAAGGAAAGTTCTGCTACTTCTCCAATCATTCAATCTCTTAAATCTTCAATTCATATGAA
ATCAAATAACAATGAACTCATCGATCAATTCAATGCTGAATCTTTAGAAAAAGATACCAC
CACCACCGATAAACTCTCCCCTGAAGGTTTGTTCTTTAAGCAACTCGGAGAAATGTTCCC
TTCCGAAGCTGATAAAGAAATTGCTATCAATACTCTCTTATATCAAAATAATAAAAATCA
AAAGATTCAAAAACTTGAAAATGATTTGGTTAATGCAATCTCTAAGGATTCCACCATTCG
TATTCCTTNTCCAAAAGAA