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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHA116
Library VH
(Link to library)
Clone ID VHA116
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10794-1
Original site URL
Representative seq. ID VHA116P
(Link to Original site)
Representative DNA sequence 
>VHA116 (VHA116Q) /CSM/VH/VHA1-A/VHA116Q.Seq.d/
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAANG
GTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCANAAAT
ATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCAA
AGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCCA
NATGAACAATTACGNGGTATTACCATAAAAGTCATCATCTGTTTCACTTCACTTTGAAAT
GCCAAAAGATGANAAATTACCAACTGGTTGTAGCTCACACNAATTCTTGATCAATCTTAT
TGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAGXXXXXXXXXXCCTAACTGA
TGATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCT
CTATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGA
ATATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGTGTTCAATATCTTCATGAAAT
TAAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGA
GAAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCA
TAGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATC
AGCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGG
TATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACG
TCGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGG
TCTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGA
TCATTGGAGTTCAATTGGTATCGTTGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATC
AATTAGAAAGAGAAAAGGTTTAAATCCAAAATTACCAACT
sequence update 2002.10.25
Translated Amino Acid sequence 
yq**illhiqnkinkt*tlffpyigqtliclctiilfkllllkfilktliskktktkkkx
silqxikfvqlwivxkisxicllllmlimvkllllih*fkelvllxirylvmqdichvvx
mnnyxvlp*KSSSVSLHFEMPKDXKLPTGCSSHXFLINLIDSPGHVDFSSEVT---

---LTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNKTKGV
QYLHEIKDSMVSAFQWISNEGVLCGEKMRSIRFNVYDVMLHADAIHRGSGQFIPTTRRVM
YASQLSAQPTLLEPMYLVDISTPESGISGIYSVLNRRRGVVIGEERRIGTPLFNIKAYLP
VMESFGLTADLRSSTAGQAFPQCVFDHWSSIGIVGQDKRSTEVASSIRKRKGLNPKLPT
Translated Amino Acid sequence (All Frames) 
Frame A:
isiiniitytkqn**nlnfifplhwpnfnlplynysl*tliikihfknshf*kn*nkkkx
vnftixqirsimdrrenixnmsviahvdhgkttltdsliqragiiaxkvsgnagymscrp
xeqlrgitikviicftsl*nakr*xitnwl*ltxildqsy*ftrsc*f*f*sn---

---pn**y**wfggf*src*kscqlsl**tflg*e*cs*hlviwsri*gskcfs*qnqrc
siss*n*rlygfsipmdlk*rcimw*eneidsfqrl*cnascrcns*r*wsiysnnsscn
vclptisstntfgtnvfg*y*ys*kwy*wyllctqs*krcchw*rtsywysiiqyqslss
cngiiwshc*ftfkycwssfpsmcf*slefnwyrwsr*kin*ssfin*kekrfkskitn

Frame B:
yq**illhiqnkinkt*tlffpyigqtliclctiilfkllllkfilktliskktktkkkx
silqxikfvqlwivxkisxicllllmlimvkllllih*fkelvllxirylvmqdichvvx
mnnyxvlp*KSSSVSLHFEMPKDXKLPTGCSSHXFLINLIDSPGHVDFSSEVT---

---LTDDIDNGLVAFNQDVKSRANYLYDKHSWDKNDAVNIWSFGPEYKGPNVLVNKTKGV
QYLHEIKDSMVSAFQWISNEGVLCGEKMRSIRFNVYDVMLHADAIHRGSGQFIPTTRRVM
YASQLSAQPTLLEPMYLVDISTPESGISGIYSVLNRRRGVVIGEERRIGTPLFNIKAYLP
VMESFGLTADLRSSTAGQAFPQCVFDHWSSIGIVGQDKRSTEVASSIRKRKGLNPKLPT

Frame C:
innkyyyiyktkliklklyfsptlakl*fafvqlfslnsyy*nsf*klsflkklkqkkxg
qfynxsnsfnygssxkyxkyvcycsc*sw*nysy*finskswyycx*giw*criyvmssx
*titxyyhkshhlfhftlkcqkmxnyqlvvahtns*sillihqvmlilvlk*q---

---*lmilimvwwllikmlkvvpiisminilgirmmqltfghlvqnirvqmf*ltkpkvf
nifmklktlwfqhsngsqmkvyyvvrk*drfvstfmm*cfmpmqfievvvnlfqqlvv*c
mppnyqlnqhfwnqciwlilvllkvvlvvstlysiveevlslvknvvlvlhysisklifl
*wnhlvslliyvqvllvklslnvfliigvqlvslvkikdqlk*lhqlerekv*iqnyq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA116 (VHA116Q) /CSM/VH/VHA1-A/VHA116Q.Seq.d/                       1996   0.0
VHN741 (VHN741Q) /CSM/VH/VHN7-B/VHN741Q.Seq.d/                       1405   0.0
VHK348 (VHK348Q) /CSM/VH/VHK3-B/VHK348Q.Seq.d/                       1405   0.0
VHD358 (VHD358Q) /CSM/VH/VHD3-C/VHD358Q.Seq.d/                       1405   0.0
VHB815 (VHB815Q) /CSM/VH/VHB8-A/VHB815Q.Seq.d/                       1405   0.0
VHB107 (VHB107Q) /CSM/VH/VHB1-A/VHB107Q.Seq.d/                       1405   0.0
SHG365 (SHG365Q) /CSM/SH/SHG3-C/SHG365Q.Seq.d/                       1405   0.0
CHS180 (CHS180Q) /CSM/CH/CHS1-D/CHS180Q.Seq.d/                       1405   0.0
AHN643 (AHN643Q) /CSM/AH/AHN6-B/AHN643Q.Seq.d/                       1405   0.0
AHM404 (AHM404Q) /CSM/AH/AHM4-A/AHM404Q.Seq.d/                       1405   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   139   e-110  10
X15386|X15386.1 Dictyostelium discoideum H6 gene mRNA.            230   2e-60  2
BI863585|BI863585.1 kx46a06.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   3e-21  4
BI863575|BI863575.1 kx45h07.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CHICK Q90705 ELONGATION FACTOR 2 ;, mRNA sequence.    66   5e-18  3
BI451383|BI451383.1 kx23e09.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:EF2_CAEEL P29691 ELONGATION FACTOR 2 ;, mRNA sequence.    66   5e-18  3
AY310285|AY310285.1 Scutigera coleoptrata voucher Scol elongation factor 2 mRNA, partial cds.    60   1e-14  3
BM056853|BM056853.1 2154-85 hindgut and Malpighian tubule subtracted cDNA library Ctenocephalides felis cDNA clone 2154-85, mRNA sequence.    92   4e-14  1
AF240831|AF240831.1 Tanystylum orbiculare elongation factor-2 mRNA, partial cds.    60   5e-14  5
DR447725|DR447725.1 AR1047E10 A. gomesiana hemocytes normalized library Acanthoscurria gomesiana cDNA clone AR1047E10 5', mRNA sequence.    88   9e-14  2
DR443579|DR443579.1 AR2018A03 A. gomesiana hemocytes library Acanthoscurria gomesiana cDNA clone AR2018A03 5', mRNA sequence.    88   9e-14  2
dna update 2007. 3.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   275   2e-72
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   272   2e-71
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   271   3e-71
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   271   4e-71
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   271   4e-71
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   271   4e-71
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   271   4e-71
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   271   4e-71
AK159514_1(AK159514|pid:none) Mus musculus osteoclast-like cell ...   271   4e-71
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   271   5e-71
protein update 2009. 6.26
PSORT 

 psg: 0.54  gvh: 0.41  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHA116 is cyt
5' end seq. ID VHA116F
5' end seq. 
>VHA116F.Seq
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAANG
GTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCANAAAT
ATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCAA
AGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCCA
NATGAACAATTACGNGGTATTACCATAAAAGTCATCATCTGTTTCACTTCACTTTGAAAT
GCCAAAAGATGANAAATTACCAACTGGTTGTAGCTCACACNAATTCTTGATCAATCTTAT
TGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAGNNNNNNNNNN
Length of 5' end seq. 531
3' end seq. ID VHA116Z
3' end seq. 
>VHA116Z.Seq
NNNNNNNNNNCCTAACTGATGATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTA
AAAGTCGTGCCAATTATCTCTATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACA
TTTGGTCATTTGGTCCAGAATATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGTG
TTCAATATCTTCATGAAATTAAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATG
AAGGTGTATTATGTGGTGAGAAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGC
TTCATGCCGATGCAATTCATAGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAA
TGTATGCCTCCCAACTATCAGCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATA
TTAGTACTCCTGAAAGTGGTATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTG
TTGTCATTGGTGAAGAACGTCGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTC
CTGTAATGGAATCATTTGGTCTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTT
TCCCTCAATGTGTTTTTGATCATTGGAGTTCAATTGGTATCGTTGGTCAAGATAAAAGAT
CAACTGAAGTAGCTTCATCAATTAGAAAGAGAAAAGGTTTAAATCCAAAATTACCAACT
Length of 3' end seq. 719
Connected seq. ID VHA116P
Connected seq. 
>VHA116P.Seq
ATATCAATAATAAATATTATTACATATACAAAACAAAATTAATAAAACTTAAACTTTATT
TTTCCCCTACATTGGCCAAACTTTAATTTGCCTTTGTACAATTATTCTCTTTAAACTCTT
ATTATTAAAATTCATTTTAAAAACTCTCATTTCTAAAAAAACTAAAACAAAAAAAAAANG
GTCAATTTTACAATANATCAAATTCGTTCAATTATGGATCGTCGNGAAAATATCANAAAT
ATGTCTGTTATTGCTCATGTTGATCATGGTAAAACTACTCTTACTGATTCATTAATTCAA
AGAGCTGGTATTATTGCANATAAGGTATCTGGTAATGCAGGATATATGTCATGTCGTCCA
NATGAACAATTACGNGGTATTACCATAAAAGTCATCATCTGTTTCACTTCACTTTGAAAT
GCCAAAAGATGANAAATTACCAACTGGTTGTAGCTCACACNAATTCTTGATCAATCTTAT
TGATTCACCAGGTCATGTTGATTTTAGTTCTGAAGTAACAG----------CCTAACTGA
TGATATTGATAATGGTTTGGTGGCTTTTAATCAAGATGTTAAAAGTCGTGCCAATTATCT
CTATGATAAACATTCTTGGGATAAGAATGATGCAGTTAACATTTGGTCATTTGGTCCAGA
ATATAAGGGTCCAAATGTTTTAGTTAACAAAACCAAAGGTGTTCAATATCTTCATGAAAT
TAAAGACTCTATGGTTTCAGCATTCCAATGGATCTCAAATGAAGGTGTATTATGTGGTGA
GAAAATGAGATCGATTCGTTTCAACGTTTATGATGTAATGCTTCATGCCGATGCAATTCA
TAGAGGTAGTGGTCAATTTATTCCAACAACTCGTCGTGTAATGTATGCCTCCCAACTATC
AGCTCAACCAACACTTTTGGAACCAATGTATTTGGTTGATATTAGTACTCCTGAAAGTGG
TATTAGTGGTATCTACTCTGTACTCAATCGTAGAAGAGGTGTTGTCATTGGTGAAGAACG
TCGTATTGGTACTCCATTATTCAATATCAAAGCTTATCTTCCTGTAATGGAATCATTTGG
TCTCACTGCTGATTTACGTTCAAGTACTGCTGGTCAAGCTTTCCCTCAATGTGTTTTTGA
TCATTGGAGTTCAATTGGTATCGTTGGTCAAGATAAAAGATCAACTGAAGTAGCTTCATC
AATTAGAAAGAGAAAAGGTTTAAATCCAAAATTACCAACT
Length of connected seq. 1230
Full length Seq ID -
Full length Seq. -
Length of full length seq. -