>VHA112 (VHA112Q) /CSM/VH/VHA1-A/VHA112Q.Seq.d/
ATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCAACAC
AAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGATTATC
AAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGTTCATGTGGAGATCGACCCAAA
GTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCAGATG
GTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGACCCAAATTTATTCGATAATA
ACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTTGAATATGCAACTCATTATC
GTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCGCTTACCTTAACGCTCAAATGT
ATTACAAAAACATTGACAAACTAATCGCACATATCAATTCAAATCCGGATAAATATGGTT
TAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTAAATGATGCCAATCTAG
TATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAATGAATTTAGTTATTGGA
CTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGTCAAAATAATGCTCTAT
TACATGTAGTTXXXXXXXXXXGTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAAT
GCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGA
AGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACT
TTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACA
ACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCT
GCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAAC
GATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCA
AATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTA
CGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACT
TTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTA
ACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAA
AACTATGATCATAAATCATCATCATCAACGAAGAAGATTCTTCAAAGGTTTTGTTTTTAC
TTTTTCTCCAATGCAAATCAGAACTTT

FGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGRMDYQDIEARLENKQMEFMWRSTQS
TPENQVFTSVLRAMYCTPDGFNFEQGDDPIQDDPNLFDNNVDSRAEQFTQVALEYATHYR
TNNVLIPFGCDFAYLNAQMYYKNIDKLIAHINSNPDKYGLNLLYSTPSIYIDAVNDANLV
WEVKTDDLFPYADNEFSYWTGYFVSRPALKGYVRQNNALLHVV---

---VVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDD
GRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNS
QYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLS
TIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKKILQRFCFYFFSN
ANQNF

Frame A:
iselcqr*agt*ihlaivqhklvslvn*dsmhslsvvwiikisklv*riskwsscgdrpk
vhqrikfshqyyvqcivlqmvstlskvmtqfkmtqiysiit*tqelnsslkwhlnmqlii
vpimy*fhlvvisltltlkcitktltn*shisiqirinmv*icyiqlhqfi*ml*mmpi*
ygk*klmisshmlimnlviglvislvvqh*katfvkimlyym*---

---VVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDD
GRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNS
QYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLS
TIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKKILQRFCFYFFSN
ANQNF

Frame B:
frsyakdrlahrsiwp*cntssylwsirircihyrsyglsryrssfre*angvhveidpk
ytressfhisttcnvlysrwfql*ar**pnsr*pkfir**rrlks*tvhssgt*icnsls
yq*ctnsiwl*frlp*rsnvlqkh*qtnrtyqfksg*iwfkfvifnsinlyrcck*cqss
mgsen**slpic***i*lldwlfr*sssierlrssk*csitcs---

---wfnqpvvimyl*mllptfkilinhynsplslievvdvhr*emvnwi**civvl*kmm
vvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigil
nikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifq
lsfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqrrrffkgfvftfspm
qirt

Frame C:
FGVMPKIGWHIDPFGHSATQARIFGQLGFDAFIIGRMDYQDIEARLENKQMEFMWRSTQS
TPENQVFTSVLRAMYCTPDGFNFEQGDDPIQDDPNLFDNNVDSRAEQFTQVALEYATHYR
TNNVLIPFGCDFAYLNAQMYYKNIDKLIAHINSNPDKYGLNLLYSTPSIYIDAVNDANLV
WEVKTDDLFPYADNEFSYWTGYFVSRPALKGYVRQNNALLHVV---

---gstnqw*lctcecyclhsrs*siitihhch**kswmcivkrwsigyddassyfer*w
swcwstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefs
isrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfn
ylfnnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiineedsskvlfllflqc
ksel

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA112 (VHA112Q) /CSM/VH/VHA1-A/VHA112Q.Seq.d/                       2730   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1417   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1417   0.0
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                       1417   0.0
VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/                       1417   0.0
VHI312 (VHI312Q) /CSM/VH/VHI3-A/VHI312Q.Seq.d/                       1417   0.0
VHH791 (VHH791Q) /CSM/VH/VHH7-D/VHH791Q.Seq.d/                       1417   0.0
VHE461 (VHE461Q) /CSM/VH/VHE4-C/VHE461Q.Seq.d/                       1417   0.0
VHE264 (VHE264Q) /CSM/VH/VHE2-C/VHE264Q.Seq.d/                       1417   0.0
VHE212 (VHE212Q) /CSM/VH/VHE2-A/VHE212Q.Seq.d/                       1417   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1330   0.0    1
AL672129|AL672129.4 Zebrafish DNA sequence from clone BUSM1-104F18 in linkage group 9  Contains a novel gene for a protein similar to KIAA1548, a novel  gene and a CpG island.    46   0.34   3
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   0.34   3
CE529546|CE529546.1 tigr-gss-dog-17000365859317 Dog Library Canis familiaris genomic, genomic survey sequence.    48   0.65   1
CR853293|CR853293.9 Zebrafish DNA sequence from clone CH211-81J7 in linkage group 9.    46   0.77   3
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   1.1    9
CR853293|CR853293.9 Zebrafish DNA sequence from clone CH211-81J7 in linkage group 9.    46   1.9    4
AM379990|AM379990.1 Oryzias latipes EST, clone FOE002-P00123-DPE-R_C22.    46   2.6    1
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   2.6    1
DD329793|DD329793.1 P-REX1, A PTDINS(3,4,5)P3-G-BETA-GAMMA-REGULATED GUANINE-NUCLEOTIDE EXCHANGE FACTOR FOR RAC.    46   2.6    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   470   e-131
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   470   e-131
BC091843_1(BC091843|pid:none) Danio rerio zgc:110815, mRNA (cDNA...   225   3e-57
BC121882_1(BC121882|pid:none) Xenopus tropicalis lysosomal alpha...   211   4e-53
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   206   1e-51
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   206   2e-51
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   204   5e-51
AY119107_1(AY119107|pid:none) Drosophila melanogaster RE08556 fu...   202   2e-50
AE014134_1633(AE014134|pid:none) Drosophila melanogaster chromos...   202   2e-50
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   201   5e-50

 psg: 0.76  gvh: 0.47  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHA112 is cyt

>VHA112F.Seq
ATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCAACAC
AAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGATTATC
AAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGTTCATGTGGAGATCGACCCAAA
GTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCAGATG
GTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGACCCAAATTTATTCGATAATA
ACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTTGAATATGCAACTCATTATC
GTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCGCTTACCTTAACGCTCAAATGT
ATTACAAAAACATTGACAAACTAATCGCACATATCAATTCAAATCCGGATAAATATGGTT
TAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTAAATGATGCCAATCTAG
TATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAATGAATTTAGTTATTGGA
CTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGTCAAAATAATGCTCTAT
TACATGTAGTTNNNNNNNNNN

>VHA112Z.Seq
NNNNNNNNNNGTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTA
CATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATG
TGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGA
TGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATT
GATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTT
ATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTC
TCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAA
GATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAA
TATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTC
AACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCA
AAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCA
TAAATCATCATCATCAACGAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAA
TGCAAATCAGAACTTT

>VHA112P.Seq
ATTTCGGAGTTATGCCAAAGATAGGCTGGCACATAGATCCATTTGGCCATAGTGCAACAC
AAGCTCGTATCTTTGGTCAATTAGGATTCGATGCATTCATTATCGGTCGTATGGATTATC
AAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGTTCATGTGGAGATCGACCCAAA
GTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTGCAATGTATTGTACTCCAGATG
GTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATGACCCAAATTTATTCGATAATA
ACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCACTTGAATATGCAACTCATTATC
GTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCGCTTACCTTAACGCTCAAATGT
ATTACAAAAACATTGACAAACTAATCGCACATATCAATTCAAATCCGGATAAATATGGTT
TAAATTTGTTATATTCAACTCCATCAATTTATATAGATGCTGTAAATGATGCCAATCTAG
TATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTGATAATGAATTTAGTTATTGGA
CTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACGTTCGTCAAAATAATGCTCTAT
TACATGTAGTT----------GTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAAT
GCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGA
AGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACT
TTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACA
ACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCT
GCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAAC
GATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCA
AATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTA
CGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACT
TTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTA
ACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAA
AACTATGATCATAAATCATCATCATCAACGAAGAAGATTCTTCAAAGGTTTTGTTTTTAC
TTTTTCTCCAATGCAAATCAGAACTTT