>VHA111 (VHA111Q) /CSM/VH/VHA1-A/VHA111Q.Seq.d/
CTGTTGGCCTACTGGCACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTT
TTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAA
AAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCA
TGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAAT
TGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCC
AGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAG
TACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAA
TGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAAT
GACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCA
CATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGA
TGCATTCATTATCGGTCGTATGGAXXXXXXXXXXCAAGGTATGGAAATGCAAAAGAGAAT
AACCAACTATCGTCCATCATGGAATTTAACCGTGGTTCAACCAACCAGTGGTAATTATGT
ACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGT
CACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCA
TCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCA
AATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTA
TCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACA
ATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTC
ACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATAC
CATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACA
AACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACCTCTGCAACTGA
AATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGT
TGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGGT
TTTGTTTTTACTTTTCTCCAATGCAAATCA

CWPTGTHIKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTH
DDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWDEQS
TTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLWENFGVMPKIGWH
IDPFGHSATQARIFGQLGFDAFIIGRM---

---QGMEMQKRITNYRPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCA
SLRDGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLS
HPLLPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENI
YQIDGQDSQDPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHK
SSSSTKEDSSKVLFLLFSNAN

Frame A:
llaywhthkngnkkiiyinflfvfnyq*n*wkknknk*y*kiktktiiniikcsyccsys
**cwlvkns**illwikyvncicrcsiyfgyshylfigksrkkvylc*ncifpkmvg*tk
ynnakyc*gfg*kwsigihkwwvlye**gyyll*ryy*pndigpsiplgefrsyakdrla
hrsiwp*cntssylwsirircihyrsyg---

---QGMEMQKRITNYRPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCA
SLRDGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLS
HPLLPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENI
YQIDGQDSQDPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHK
SSSSTKEDSSKVLFLLFSNAN

Frame B:
CWPTGTHIKMVIKKLFILIFCLFLIINEINGKKTKINDIKKSKPKLSSTLLNVHIVAHTH
DDVGWLKTVDEYYYGSNMSIAFAGVQYTLDTAITCLLANPERKFIYVEIAFFQRWWDEQS
TTMQNIVKGLVESGQLEFINGGYCMNDEATTYYDDTIDQMTLGHQFLWENFGVMPKIGWH
IDPFGHSATQARIFGQLGFDAFIIGRM---

---kvwkckre*ptivhhgi*pwfnqpvvimyl*mllptfkilinhynsplslievvdvh
r*emvnwi**civvl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyh
ihysqclqplnnlqtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkif
iklmvkihkihklsl*ifqlsfqqlqsplqlk*i*lvfknyqiyqd*ngkllmvktmiin
hhhqlkkilqrfcfyfspmqi

Frame C:
vgllaht*kw**knyly*ffvcf*lsmklmekkqk*milknqnqnyhqhy*mfilllilm
mmlvg*kqlmniimdqicqlhlqvfnilwiqplpvywqiqkeslfmlklhfskdggmnkv
qqckillrvwlkvvnwns*mvgiv*mmrllptmtilltk*hwainsfgriselcqr*agt
*ihlaivqhklvslvn*dsmhslsvvw---

---rygnakennqlssimefnrgstnqw*lctcecyclhsrs*siitihhch**kswmci
vkrwsigyddassyfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfit
sitpnvynhstifkrlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kyl
sn*wsrftrstnyhfrsfnylfnnynhlcn*nefnwcskiikfikikmenc*w*kl*s*i
iiin*rrffkgfvftflqcks

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA111 (VHA111Q) /CSM/VH/VHA1-A/VHA111Q.Seq.d/                       2466   0.0
CHL350 (CHL350Q) /CSM/CH/CHL3-C/CHL350Q.Seq.d/                       1530   0.0
VHC754 (VHC754Q) /CSM/VH/VHC7-C/VHC754Q.Seq.d/                       1524   0.0
VHC576 (VHC576Q) /CSM/VH/VHC5-D/VHC576Q.Seq.d/                       1520   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1516   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1516   0.0
SHD822 (SHD822Q) /CSM/SH/SHD8-A/SHD822Q.Seq.d/                       1516   0.0
SHD680 (SHD680Q) /CSM/SH/SHD6-D/SHD680Q.Seq.d/                       1516   0.0
SHA621 (SHA621Q) /CSM/SH/SHA6-A/SHA621Q.Seq.d/                       1516   0.0
AHM441 (AHM441Q) /CSM/AH/AHM4-B/AHM441Q.Seq.d/                       1516   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1433   0.0    2
CK149800|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.   117   2e-23  3
DN814237|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-16  4
DN816506|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   2e-16  4
CX633815|CX633815.1 taj44a03.y3 Hydra EST UCI 5 Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   3e-16  4
DN816232| truncated lysosomal acid alpha-mannosidase [Homo sapiens], mRNA sequence.    52   4e-16  4
DN811862| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   4e-16  4
DT613126|A Chain A, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation, mRNA sequence.    52   4e-16  4
DN815247| lysosomal alpha-mannosidase [Cavia porcellus], mRNA sequence.    52   4e-16  4
CB073433|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence.    52   3e-15  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   409   e-112
M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   406   e-111
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   213   2e-53
U05572_1(U05572|pid:none) Human lysosomal alpha-mannosidase (MAN...   213   2e-53
AB209921_1(AB209921|pid:none) Homo sapiens mRNA for mannosidase,...   213   2e-53
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   211   4e-53
JC2200(JC2200)alpha-mannosidase (EC 3.2.1.24) precursor - human       211   5e-53
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   209   2e-52
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   207   7e-52
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   207   7e-52

 psg: 0.77  gvh: 0.62  alm: 0.17  top: 0.43  tms: 0.07  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 12.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHA111 is end

>VHA111F.Seq
CTGTTGGCCTACTGGCACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTT
TTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAA
AAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCA
TGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAAT
TGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCC
AGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAG
TACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAA
TGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAAT
GACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCA
CATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGA
TGCATTCATTATCGGTCGTATGGANNNNNNNNNN

>VHA111Z.Seq
NNNNNNNNNNCAAGGTATGGAAATGCAAAAGAGAATAACCAACTATCGTCCATCATGGAA
TTTAACCGTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACAT
TCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGC
ATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGG
TCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGAT
TTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATC
ACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCA
ATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGAT
TCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATAT
TTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAAC
TATCTTTTCAACAATTACAATCACCTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAA
ATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAA
ATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTCTCCAATGC
AAATCA

>VHA111P.Seq
CTGTTGGCCTACTGGCACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTT
TTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAA
AAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCA
TGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAAT
TGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCC
AGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAG
TACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAA
TGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAAT
GACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCA
CATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGA
TGCATTCATTATCGGTCGTATGGA----------CAAGGTATGGAAATGCAAAAGAGAAT
AACCAACTATCGTCCATCATGGAATTTAACCGTGGTTCAACCAACCAGTGGTAATTATGT
ACCTGTGAATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGT
CACTGATAGAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCA
TCGTCGTACTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCA
AATCGTTACAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTA
TCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACA
ATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTC
ACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATAC
CATACTTTTACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACA
AACTATCACTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACCTCTGCAACTGA
AATGAATTTAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGT
TGATGGTAAAAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGGT
TTTGTTTTTACTTTTCTCCAATGCAAATCA