>VHA110 (VHA110Q) /CSM/VH/VHA1-A/VHA110Q.Seq.d/
XXXXXXXXXXGGTATGGAAATGCAAAAGAGAATANCCAACTATCGTCCATCATGGAATTT
AACCGTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCA
AGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATC
GTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCG
TGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTT
CCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACA
TCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATA
TCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCA
AACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTA
TCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTAT
CTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATT
ATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACT

---MEMQKRIXNYRPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASL
RDGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHP
LLPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQ
IDGQDSQDPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKN

Frame A:
---vwkckrexptivhhgi*pwfnqpvvimyl*mllptfkilinhynsplslievvdvhr
*emvnwi**civvl*kmmvvvlvnq*msqlkslqlvn*fsmifqvmlnliivllhslyhi
hysqclqplnnlqtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifi
klmvkihkihklsl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvkt


Frame B:
---ygnakenxqlssimefnrgstnqw*lctcecyclhsrs*siitihhch**kswmciv
krwsigyddassyfer*wswcwstne*vnsnryn**idfp*yfklcsislsscctlfits
itpnvynhstifkrlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kyls
n*wsrftrstnyhfrsfnylfnnynhfcn*nefnwcskiikfikikmenc*w*k


Frame C:
---MEMQKRIXNYRPSWNLTVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASL
RDGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHP
LLPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQ
IDGQDSQDPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKN

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1376   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1376   0.0
VHC754 (VHC754Q) /CSM/VH/VHC7-C/VHC754Q.Seq.d/                       1376   0.0
VHB425 (VHB425Q) /CSM/VH/VHB4-B/VHB425Q.Seq.d/                       1376   0.0
VHA110 (VHA110Q) /CSM/VH/VHA1-A/VHA110Q.Seq.d/                       1376   0.0
VFM747 (VFM747Q) /CSM/VF/VFM7-B/VFM747Q.Seq.d/                       1376   0.0
SHD822 (SHD822Q) /CSM/SH/SHD8-A/SHD822Q.Seq.d/                       1376   0.0
SHD680 (SHD680Q) /CSM/SH/SHD6-D/SHD680Q.Seq.d/                       1376   0.0
SHD435 (SHD435Q) /CSM/SH/SHD4-B/SHD435Q.Seq.d/                       1376   0.0
SHC216 (SHC216Q) /CSM/SH/SHC2-A/SHC216Q.Seq.d/                       1376   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1348   0.0    1
AP007341|AP007341.1 Lotus japonicus genomic DNA, chromosome 2, clone:LjT35G04, TM0425.    40   0.12   2
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   0.12   8
EG619833|EG619833.1 EMBRYOF088227O8 POSSUM_01-POSSUM-C-EMBRYO-2KB Trichosurus vulpecula cDNA clone 1061028989863, mRNA sequence.    46   1.3    1
AM025232|AM025232.1 Bos indicus 3' EST, clone C0005915l21.         34   3.3    2
BH703301|BH703301.1 BOMNG55TR BO_2_3_KB Brassica oleracea genomic clone BOMNG55, DNA sequence.    40   3.7    2
CT485998|CT485998.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-104K5.    32   3.8    6
AA382162|AA382162.1 EST95325 Activated T-cells II Homo sapiens cDNA 5' end, mRNA sequence.    40   4.4    2
EH243429|EH243429.1 JGI_ACAC13551.fwd ACAC Phakopsora pachyrhizi TW72-1 germinating urediniospores on water surface (20C/16 hours/dark) Phakopsora pachyrhizi cDNA clone ACAC13551 5', mRNA sequence.    44   5.0    1
AV262947|AV262947.1 Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930432P20, 3' end partial sequence.    44   5.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   397   e-109
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   397   e-109
BT036325_1(BT036325|pid:none) Zea mays full-length cDNA clone ZM...   108   2e-22
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   107   3e-22
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   106   6e-22
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   102   1e-20
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   102   1e-20
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   101   2e-20
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   100   4e-20
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   100   6e-20

 psg: 0.82  gvh: 0.51  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHA110 is nuc

>VHA110Z.Seq
NNNNNNNNNNGGTATGGAAATGCAAAAGAGAATANCCAACTATCGTCCATCATGGAATTT
AACCGTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCA
AGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATC
GTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCG
TGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTT
CCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACA
TCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATA
TCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCA
AACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTA
TCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTAT
CTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATT
ATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACT