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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHA104
Library VH
(Link to library)
Clone ID VHA104
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHA104P
(Link to Original site)
Representative DNA sequence 
>VHA104 (VHA104Q) /CSM/VH/VHA1-A/VHA104Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAGTGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAXXXXXXXXXXGTTGGTGCTTTCCGATGGGCCACCAAAGAAGGTGTTGTTTGTG
ATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACGTGATGCC
ATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAA
TTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAA
AACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAA
GAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCC
TTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTC
TTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCT
TTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCAC
GAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATCAAATCTAA
AAAAAATTTTAAAAAAAGAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEVPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---VGAFRWATKEGVVCDENMRGIRFNLYDVTLHT*chpqrwwsnhpncssctlcc*inc
lpnslrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrf
hr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprkt
lnnlsrtlsfqx**snlkkilkke
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---VGAFRWATKEGVVCDENMRGIRFNLYDVTLHT*chpqrwwsnhpncssctlcc*inc
lpnslrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrf
hr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprkt
lnnlsrtlsfqx**snlkkilkke

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrsakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---lvlsdgppkkvlfvmkt*evsvsifmmspstrdaihrgggqiiptarrvlyaaelta
sptllepiylveitapenaiggiysvlnrrrgivigeerrigsplfsvkahlpvlesfgf
tadlrshtagqafpqcvfdhwasigvvnkdkkatevalatrkrkglapeipdldkfhekl
*ti*val*vfkxsnqi*kkf*kk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEVPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---wcfpmghqrrccl**kherypfqsl*chpphvmpstevvvkssqllvvysmlln*lp
pqls*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvs
pliyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstkns
kqfkshfefsxivikskknfkkr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHA104 (VHA104Q) /CSM/VH/VHA1-A/VHA104Q.Seq.d/                       2270   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1156   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1156   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1156   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1156   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1156   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1156   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1156   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1156   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.  1154   0.0    1
AF107291|AF107291.1 Candida parapsilosis elongation factor 2 (EFT2) gene, partial cds.    70   1e-57  12
AF107286|AF107286.1 Candida albicans elongation factor 2 (EFT2) gene, partial cds.    78   2e-54  8
Y09664|Y09664.1 C.albicans EFT2 gene.                              78   2e-53  8
AF107289|AF107289.1 Candida tropicalis elongation factor 2 (EFT2) gene, partial cds.    62   3e-44  8
AF534908|AF534908.2 Tetrahymena thermophila elongation factor 2 gene, complete cds.    84   2e-43  5
AF107290|AF107290.1 Candida lusitaniae elongation factor 2 (EFT2) gene, partial cds.    62   6e-35  6
BW270068|BW270068.1 Ciona intestinalis cDNA, clone:cign055h19, 5' end, single read.   101   2e-33  4
AV986603|AV986603.1 Ciona intestinalis cDNA, clone:cilv42g15, 5' end, single read.   101   3e-33  4
AV842050|AV842050.1 Ciona intestinalis cDNA, clone:rcieg07b05, 3' end, single read.   101   3e-33  4
dna update 2007. 3.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   263   9e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   258   3e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   257   5e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   1e-66
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   256   1e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   255   2e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   3e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   3e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   4e-66
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   254   4e-66
protein update 2009. 6.26
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHA104 is cyt
5' end seq. ID VHA104F
5' end seq. 
>VHA104F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAGTGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGANNNNNNNNNN
Length of 5' end seq. 617
3' end seq. ID VHA104Z
3' end seq. 
>VHA104Z.Seq
NNNNNNNNNNGTTGGTGCTTTCCGATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACGTGATGCCATCCACA
GAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATCAAATCTAAAAAAAAT
TTTAAAAAAAGAA
Length of 3' end seq. 613
Connected seq. ID VHA104P
Connected seq. 
>VHA104P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAGTGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGA----------GTTGGTGCTTTCCGATGGGCCACCAAAGAAGGTGTTGTTTGTG
ATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACGTGATGCC
ATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAA
TTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAA
AACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAA
GAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCC
TTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTC
TTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCT
TTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCAC
GAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATCAAATCTAA
AAAAAATTTTAAAAAAAGAA
Length of connected seq. 1210
Full length Seq ID -
Full length Seq. -
Length of full length seq. -