>VFO833 (VFO833Q) /CSM/VF/VFO8-B/VFO833Q.Seq.d/
XXXXXXXXXXCAATCAAACTTCATTCCAACAAAACCTTGCCAATCGTTCAGTTGAAGCCG
AAACCGTCTCCAAAACAGGTAAATCTTATGCTTTCAGTGGTTCAACTTCAACCTTACCAG
CCTTCATTCGTGTTCCAGGTGCCCTTAAAACTGATACCATTGTTTTTGATTCTGTCAATG
GATGGCAACAAGTCGATGTAGTAGGTGCATCACCAACCCCAAGAGCTCATCATCATTTAC
ACTATAATGAAGAATTAGATTCAATTTTCACCTATGGCGGCTATACCAATTGTAGTACCT
ACACCACCAATGAATGTAATTATCTTGGCGATTTATGGAGTCTTGATTTATCAACAAAAG
TTTGGAAGCAACATCTCTTTGATGTAACCAAAGGACCAGGAAATAGAGATAATGCCAAAT
TCATTTATTCCGATCAAAATCAACTTTTCTTAATTGGTGGAAGTAATTATCAATACACCA
ACTTTATGGATGTATGGCGTTTTGATTTAATTACAAATGAATGGACTCAATTAACCATTT
TACCATTAGTTGATCCAGAAACTTATATTCAAACTCCAATTCCAAGTACAATCGGTAGCT
TCTATTTCAAACATATCAGAAAATGTAGTGTAGAATTTACTTTAGCAGAGGTTCACTCAG
TAAATTTCAGGAAGTCCAT

---NQTSFQQNLANRSVEAETVSKTGKSYAFSGSTSTLPAFIRVPGALKTDTIVFDSVNG
WQQVDVVGASPTPRAHHHLHYNEELDSIFTYGGYTNCSTYTTNECNYLGDLWSLDLSTKV
WKQHLFDVTKGPGNRDNAKFIYSDQNQLFLIGGSNYQYTNFMDVWRFDLITNEWTQLTIL
PLVDPETYIQTPIPSTIGSFYFKHIRKCSVEFTLAEVHSVNFRKS

Frame A:
---NQTSFQQNLANRSVEAETVSKTGKSYAFSGSTSTLPAFIRVPGALKTDTIVFDSVNG
WQQVDVVGASPTPRAHHHLHYNEELDSIFTYGGYTNCSTYTTNECNYLGDLWSLDLSTKV
WKQHLFDVTKGPGNRDNAKFIYSDQNQLFLIGGSNYQYTNFMDVWRFDLITNEWTQLTIL
PLVDPETYIQTPIPSTIGSFYFKHIRKCSVEFTLAEVHSVNFRKS


Frame B:
---iklhsnktlpivqlkpkpspkqvnlmlsvvqlqpyqpsfvfqvplkliplflilsmd
gnksm**vhhqpqeliiiytimkn*iqfspmaaipivvptppmnviilaiygvliyqqkf
gsnislm*pkdqeieimpnsfipikinfs*lveviintptlwmygvli*lqmngln*pfy
h*liqklifklqfqvqsvasisnisenvv*nll*qrftq*isgsp


Frame C:
---snfiptkpcqsfs*srnrlqnr*ilcfqwfnfnltslhscsrcp*n*yhcf*fcqwm
atsrcsrcitnpkssssftl**rirfnfhlwrlyql*ylhhq*m*lswrfmes*finksl
eatsl*cnqrtrk*r*cqihlfrskstflnwwk*lsihqlygcmaf*fnyk*mdsinhft
is*srnlysnsnskynr*llfqtyqkm*criyfsrgslskfqevh

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO833 (VFO833Q) /CSM/VF/VFO8-B/VFO833Q.Seq.d/                       1326   0.0
VFB257 (VFB257Q) /CSM/VF/VFB2-C/VFB257Q.Seq.d/                       1320   0.0
VFA714 (VFA714Q) /CSM/VF/VFA7-A/VFA714Q.Seq.d/                       1314   0.0
VFJ880 (VFJ880Q) /CSM/VF/VFJ8-D/VFJ880Q.Seq.d/                       1312   0.0
VFG609 (VFG609Q) /CSM/VF/VFG6-A/VFG609Q.Seq.d/                       1312   0.0
VFF560 (VFF560Q) /CSM/VF/VFF5-C/VFF560Q.Seq.d/                       1312   0.0
VFD390 (VFD390Q) /CSM/VF/VFD3-D/VFD390Q.Seq.d/                       1312   0.0
VFD781 (VFD781Q) /CSM/VF/VFD7-D/VFD781Q.Seq.d/                       1298   0.0
AFM105 (AFM105Q) /CSM/AF/AFM1-A/AFM105Q.Seq.d/                       1296   0.0
VFL455 (VFL455Q) /CSM/VF/VFL4-C/VFL455Q.Seq.d/                       1292   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ436176) Dictyostelium discoideum cDNA clone:ddv30b10, 3' ...  1326   0.0    1
(BJ429439) Dictyostelium discoideum cDNA clone:ddv3a16, 3' e...  1320   0.0    1
(BJ429036) Dictyostelium discoideum cDNA clone:ddv2l03, 3' e...  1314   0.0    1
(BJ433163) Dictyostelium discoideum cDNA clone:ddv20p20, 3' ...  1312   0.0    1
(BJ433317) Dictyostelium discoideum cDNA clone:ddv21n07, 3' ...  1287   0.0    1
(BJ432741) Dictyostelium discoideum cDNA clone:ddv19k14, 3' ...  1245   0.0    3
(BJ431381) Dictyostelium discoideum cDNA clone:ddv14a04, 3' ...  1245   0.0    2
(BJ430580) Dictyostelium discoideum cDNA clone:ddv7d23, 3' e...  1245   0.0    2
(BJ428604) Dictyostelium discoideum cDNA clone:ddv12g15, 3' ...  1245   0.0    2
(AC116551) Dictyostelium discoideum chromosome 2 map complem...  1237   0.0    3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116551_43(AC116551|pid:none) Dictyostelium discoideum chromoso...   437   e-121
AM746676_5484(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...   156   5e-37
BC012312_1(BC012312|pid:none) Mus musculus kelch domain containi...    54   6e-06
BC058359_1(BC058359|pid:none) Mus musculus kelch domain containi...    54   6e-06
(Q921I2) RecName: Full=Kelch domain-containing protein 4;  &BC02...    54   6e-06
BC062489_1(BC062489|pid:none) Xenopus tropicalis kelch domain co...    52   1e-05
AB055250_1(AB055250|pid:none) Macaca fascicularis brain cDNA, cl...    52   2e-05
AK023986_1(AK023986|pid:none) Homo sapiens cDNA FLJ13924 fis, cl...    51   3e-05
(Q8TBB5) RecName: Full=Kelch domain-containing protein 4;  &BC02...    51   3e-05
BC001044_1(BC001044|pid:none) Homo sapiens kelch domain containi...    51   3e-05

 psg: 0.72  gvh: 0.42  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VFO833 is nuc

>VFO833Z.Seq
NNNNNNNNNNCAATCAAACTTCATTCCAACAAAACCTTGCCAATCGTTCAGTTGAAGCCG
AAACCGTCTCCAAAACAGGTAAATCTTATGCTTTCAGTGGTTCAACTTCAACCTTACCAG
CCTTCATTCGTGTTCCAGGTGCCCTTAAAACTGATACCATTGTTTTTGATTCTGTCAATG
GATGGCAACAAGTCGATGTAGTAGGTGCATCACCAACCCCAAGAGCTCATCATCATTTAC
ACTATAATGAAGAATTAGATTCAATTTTCACCTATGGCGGCTATACCAATTGTAGTACCT
ACACCACCAATGAATGTAATTATCTTGGCGATTTATGGAGTCTTGATTTATCAACAAAAG
TTTGGAAGCAACATCTCTTTGATGTAACCAAAGGACCAGGAAATAGAGATAATGCCAAAT
TCATTTATTCCGATCAAAATCAACTTTTCTTAATTGGTGGAAGTAATTATCAATACACCA
ACTTTATGGATGTATGGCGTTTTGATTTAATTACAAATGAATGGACTCAATTAACCATTT
TACCATTAGTTGATCCAGAAACTTATATTCAAACTCCAATTCCAAGTACAATCGGTAGCT
TCTATTTCAAACATATCAGAAAATGTAGTGTAGAATTTACTTTAGCAGAGGTTCACTCAG
TAAATTTCAGGAAGTCCAT