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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFO725
Library VF
(Link to library)
Clone ID VFO725
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFO725 (VFO725Q) /CSM/VF/VFO7-B/VFO725Q.Seq.d/
CAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCGTTTCCGATCAAGCT
GCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCC
AACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTA
CCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCA
TTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATG
ATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCGTATGGGTTCAGAAGTTTAT
CATAACTTAAAGAATGTCGTCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGAT
GAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTT
GCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCT
GAATTCAAAGTTGAAAATGGTTACGATCTTGACXXXXXXXXXX
sequence update 2001.11.22
Translated Amino Acid sequence 
qkhsrshstsrhw*IVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSLAVCRAGAADRNL
PLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLAMQEFMILPVGAKDFNEAYRMGSEVY
HNLKNVVSGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAAS
EFKVENGYDL---
Translated Amino Acid sequence (All Frames) 
Frame A:
qkhsrshstsrhw*IVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSLAVCRAGAADRNL
PLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLAMQEFMILPVGAKDFNEAYRMGSEVY
HNLKNVVSGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAAS
EFKVENGYDL---


Frame B:
knileviqpavigksfpikllsin**sismvpqikvn*vptlslqsv*lfaelvllieiy
hftnisqklqvlk*dyqfqhsmlsmvvhmqvin*lcknl*fyqlvlkismkpivwvqkfi
it*rmssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlpl
nsklkmvtil---


Frame C:
ktfsksfnqpslvnrfrsscyr*indrsrwypk*r*ircqrypcsqfsclqswcc**kft
tlqiylrncry*neitsssiqcyqwwftcr**isyariydftswc*rfq*slsygfrsls
*lkecrqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl*
iqs*kwlrs*---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO725 (VFO725Q) /CSM/VF/VFO7-B/VFO725Q.Seq.d/                       1136   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1100   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                        817   0.0
AFD204 (AFD204Q) /CSM/AF/AFD2-A/AFD204Q.Seq.d/                        813   0.0
SFC211 (SFC211Q) /CSM/SF/SFC2-A/SFC211Q.Seq.d/                        805   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                        761   0.0
AFE445 (AFE445Q) /CSM/AF/AFE4-B/AFE445Q.Seq.d/                        761   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                        759   0.0
AFO860 (AFO860Q) /CSM/AF/AFO8-C/AFO860Q.Seq.d/                        759   0.0
AFG235 (AFG235Q) /CSM/AF/AFG2-B/AFG235Q.Seq.d/                        759   0.0
own update 2003. 6.23
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ417329) Dictyostelium discoideum cDNA clone:ddv30b07, 5' ...  1136   0.0    1
(AU060264) Dictyostelium discoideum slug cDNA, clone SLA770.      783   0.0    3
(BJ324596) Dictyostelium discoideum cDNA clone:dda7g02, 5' e...   737   0.0    2
(BJ387795) Dictyostelium discoideum cDNA clone:dds5e04, 5' e...   729   0.0    2
(BJ325039) Dictyostelium discoideum cDNA clone:dda9j12, 5' e...   686   0.0    2
(BJ391172) Dictyostelium discoideum cDNA clone:dds15n09, 5' ...   686   0.0    2
(BJ329054) Dictyostelium discoideum cDNA clone:dda30h16, 5' ...   684   0.0    2
(BJ411466) Dictyostelium discoideum cDNA clone:ddv4f14, 5' e...   684   0.0    2
(BJ325213) Dictyostelium discoideum cDNA clone:dda13e10, 5' ...   684   0.0    2
(BJ329066) Dictyostelium discoideum cDNA clone:dda30j18, 5' ...   682   0.0    2
dna update 2009. 6. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   351   1e-98
A53665(A53665) phosphopyruvate hydratase (EC 4.2.1.11) - liver f...   264   1e-69
AM279157_1(AM279157|pid:none) Echinostoma caproni mRNA for enola...   254   9e-67
DQ869009_1(DQ869009|pid:none) Echinostoma caproni enolase mRNA, ...   254   9e-67
CP000581_159(CP000581|pid:none) Ostreococcus lucimarinus CCE9901...   250   1e-65
AF258668_1(AF258668|pid:none) Polyxenus fasciculatus enolase mRN...   250   2e-65
AY522931_1(AY522931|pid:none) Diplopoda sp. SBH266145 2-phospho-...   250   2e-65
DQ994681_1(DQ994681|pid:none) Echiurus echiurus alpha enolase ge...   249   4e-65
CR954201_155(CR954201|pid:none) Ostreococcus tauri strain OTTH05...   248   4e-65
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   249   5e-65
protein update 2009. 6.26
PSORT 

 psg: 0.75  gvh: 0.72  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFO725 is cyt
5' end seq. ID VFO725F
5' end seq. 
>VFO725F.Seq
CAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAATCGTTTCCGATCAAGCT
GCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCC
AACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTA
CCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCA
TTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATTAGCTATGCAAGAATTTATG
ATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCGTATGGGTTCAGAAGTTTAT
CATAACTTAAAGAATGTCGTCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGAT
GAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTT
GCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCT
GAATTCAAAGTTGAAAATGGTTACGATCTTGACNNNNNNNNNN
Length of 5' end seq. 583
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -