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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFO635
Library VF
(Link to library)
Clone ID VFO635
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U10300-1
Original site URL
Representative seq. ID VFO635E
(Link to Original site)
Representative DNA sequence 
>VFO635 (VFO635Q) /CSM/VF/VFO6-B/VFO635Q.Seq.d/
AAAAAAAATAAAAAATAATAAGAATGGGAGTCTTAACTGATATATCATTATTGTTGACCA
ATATAATTGGTTCAATAATTCTATTAATATTTTATCCTATATTAGGTTTTTTAAATTATA
AAAAAAAAGAAAACAAATTTCCCAACACCAAAAGCAATAGTTATCACGGGTGCATCATCT
GGTATTGGTAGAGGAATAGCACTTGAATATGCAAAAAGAAAAAAAAATAACTCTTTGGTT
TTAGGTTTAACTGGAAGAAACATTGAAAAATTAGAAGAAATTCAAAAAGAATGTTTGGGT
CTTGGNGTTCAAGTANAAATTGAATCAATCGATGTCACTGNCAAAGAAAAATTAAATGAT
TGGTTAATTAAATTTGACAATAAATATAAAATTGATATTTTAATTGCAAATGCTGGAACT
ATTGAAACAATTTTACCAAAAGAATTAGATTTTACTGAAAGAATATTAACAGTTGCAAAC
ACAAATGTAATTGGAACATTGAATACAGTTTTACCAATGGTACCAATCTTTGAATCAAGA
GGATCTGGTCAATTGGTTTTGATGTCAAGTATTACACCATATTTTGATTATGTAATGGCA
GGATATTCATCAAGTAAAGGTTACATAAAGTCATTTGGGTTAATACTTAGAAATGGTTTA
GCATCAAGAGGCGTTGGCGTTTCAGTAATAACACCAGGTTTTATTTATACTCCATTAGTT
GATTCTTTGGATTATAAGGATATACCTATGTTACTACCTATAACAACTGACAAAGCTTCA
AAATACATTGTCGATGGCATTAGTAGAAATGATGCAATAATTTCATTCCCACCTTTAATT
TATTGTATATCTCATTTCGTTGCCACAATTCCACCAACTTTAAGAGATGCCACTAATTTC
ATTTCAAATTCTCTTTTGAAATATCCAGATTATAGTAAACCAGTAACTCATTATTCTCAT
CACTCTCAATATTCAACAAATTAATCAAAAAGAGAGGATTGTCCCTCCTCACCAGCTANA
ATTCAAATTCTCAAAAAAATAAAGAGGCA
sequence update 2001.11.24
Translated Amino Acid sequence 
kknkk**ewes*liyhyc*pi*lvq*fy*yfily*vf*IIKKKKTNFPTPKAIVITGASS
GIGRGIALEYAKRKKNNSLVLGLTGRNIEKLEEIQKECLGLGVQVXIESIDVTXKEKLND
WLIKFDNKYKIDILIANAGTIETILPKELDFTERILTVANTNVIGTLNTVLPMVPIFESR
GSGQLVLMSSITPYFDYVMAGYSSSKGYIKSFGLILRNGLASRGVGVSVITPGFIYTPLV
DSLDYKDIPMLLPITTDKASKYIVDGISRNDAIISFPPLIYCISHFVATIPPTLRDATNF
ISNSLLKYPDYSKPVTHYSHHSQYSTN*skredcpsspaxiqilkkikr
Translated Amino Acid sequence (All Frames) 
Frame A:
kknkk**ewes*liyhyc*pi*lvq*fy*yfily*vf*IIKKKKTNFPTPKAIVITGASS
GIGRGIALEYAKRKKNNSLVLGLTGRNIEKLEEIQKECLGLGVQVXIESIDVTXKEKLND
WLIKFDNKYKIDILIANAGTIETILPKELDFTERILTVANTNVIGTLNTVLPMVPIFESR
GSGQLVLMSSITPYFDYVMAGYSSSKGYIKSFGLILRNGLASRGVGVSVITPGFIYTPLV
DSLDYKDIPMLLPITTDKASKYIVDGISRNDAIISFPPLIYCISHFVATIPPTLRDATNF
ISNSLLKYPDYSKPVTHYSHHSQYSTN*skredcpsspaxiqilkkikr


Frame B:
kkiknnkngsln*yiiivdqynwfnnsinilsyirffkl*kkrkqisqhqkq*lsrvhhl
vlvee*hlnmqkekkitlwf*v*leetlkn*kkfkknvwvlxfkxklnqsmslxkkn*mi
g*lnltiniklif*lqmlellkqfyqkn*illkey*qlqtqm*leh*iqfyqwyqslnqe
dlvnwf*cqvlhhilim*wqdihqvkvt*shlg*ylemv*hqealafq**hqvlfilh*l
ilwiiriylcyyl*qltklqntlsmalvemmq*fhshl*fivylislpqfhql*emplis
fqilf*niqiivnq*liilitlniqqinqkerivpphqlxfkfskk*rg


Frame C:
kk*kiirmgvltdisllltniigsiillifypilgflnykkkenkfpntksnsyhgciiw
yw*rnst*ickkkkk*lfgfrfnwkkh*kirrnskrmfgswxssxn*inrchxqrkik*l
vn*i*q*i*n*yfnckcwny*nnftkrirfy*kninsckhkcnwnieysftngtnl*ikr
iwsigfdvkyytif*lcngrifik*rlhkviwvnt*kwfsikrrwrfsnntrfylysis*
ffgl*gytyvttynn*qsfkihcrwh**k*cnnfiptfnllyisfrchnstnfkrch*fh
fkfsfeisrl**tsnslfsslsifnklikkrglslltsxnsnsqknkea
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO635 (VFO635Q) /CSM/VF/VFO6-B/VFO635Q.Seq.d/                       1572   0.0
VFL652 (VFL652Q) /CSM/VF/VFL6-C/VFL652Q.Seq.d/                       1273   0.0
VFL532 (VFL532Q) /CSM/VF/VFL5-B/VFL532Q.Seq.d/                       1251   0.0
SSF546 (SSF546Q) /CSM/SS/SSF5-B/SSF546Q.Seq.d/                        940   0.0
SSD456 (SSD456Q) /CSM/SS/SSD4-C/SSD456Q.Seq.d/                        769   0.0
VFH209 (VFH209Q) /CSM/VF/VFH2-A/VFH209Q.Seq.d/                        704   0.0
VFD335 (VFD335Q) /CSM/VF/VFD3-B/VFD335Q.Seq.d/                         40   0.067
SFD858 (SFD858Q) /CSM/SF/SFD8-C/SFD858Q.Seq.d/                         40   0.067
CHL686 (CHL686Q) /CSM/CH/CHL6-D/CHL686Q.Seq.d/                         38   0.26
AFD888 (AFD888Q) /CSM/AF/AFD8-D/AFD888Q.Seq.d/                         38   0.26
own update 2002.12.20
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ436189) Dictyostelium discoideum cDNA clone:ddv30e10, 3' ...  1225   0.0    1
(BJ434656) Dictyostelium discoideum cDNA clone:ddv24g14, 3' ...  1172   0.0    1
(AC117072) Dictyostelium discoideum chromosome 2 map 3879572...  1039   0.0    7
(BJ434617) Dictyostelium discoideum cDNA clone:ddv24o07, 3' ...   928   0.0    1
(BJ413296) Dictyostelium discoideum cDNA clone:ddv15a04, 5' ...   357   0.0    4
(BJ415986) Dictyostelium discoideum cDNA clone:ddv24o07, 5' ...   410   0.0    4
(BJ417346) Dictyostelium discoideum cDNA clone:ddv30e10, 5' ...   236   0.0    4
(C84761) Dictyostelium discoideum slug cDNA, clone SSF546.        531   e-146  1
(AU072116) Dictyostelium discoideum slug cDNA, clone SSD456.      420   e-113  1
(AU072959) Dictyostelium discoideum slug cDNA, clone SSF546.      383   e-101  1
dna update 2009. 5.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BX571965_2817(BX571965|pid:none) Burkholderia pseudomallei strai...   144   4e-33
CP000010_1992(CP000010|pid:none) Burkholderia mallei ATCC 23344 ...   144   4e-33
CP001016_281(CP001016|pid:none) Beijerinckia indica subsp. indic...   144   7e-33
AF285636_24(AF285636|pid:none) Burkholderia mallei capsule gene ...   144   7e-33
CP000958_746(CP000958|pid:none) Burkholderia cenocepacia MC0-3 c...   143   9e-33
CP000613_1515(CP000613|pid:none) Rhodospirillum centenum SW, com...   143   1e-32
CP000086_1326(CP000086|pid:none) Burkholderia thailandensis E264...   136   1e-30
AM747720_3230(AM747720|pid:none) Burkholderia cenocepacia J2315 ...   136   1e-30
CP000458_779(CP000458|pid:none) Burkholderia cenocepacia HI2424 ...   132   2e-29
CP001510_2312(CP001510|pid:none) Methylobacterium extorquens AM1...   128   3e-28
protein update 2009. 7.11
PSORT 

 psg: 0.69  gvh: 0.40  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.09
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VFO635 is nuc
5' end seq. ID VFO635F
5' end seq. 
>VFO635F.Seq
AAAAAAAATAAAAAATAATAAGAATGGGAGTCTTAACTGATATATCATTATTGTTGACCA
ATATAATTGGTTCAATAATTCTATTAATATTTTATCCTATATTAGGTTTTTTAAATTATA
AAAAAAAAGAAAACAAATTTCCCAACACCAAAAGCAATAGTTATCACGGGTGCATCATCT
GGTATTGGTAGAGGAATAGCACTTGAATATGCAAAAAGAAAAAAAAATAACTCTTTGGTT
TTAGGTTTAACTGGAAGAAACATTGAAAAATTAGAAGAAATTCAAAAAGAATGTTTGGGT
CTTGGNGTTCAAGTANAAATTGAATCAATCGATGTCACTGNCAAAGAAAAATTAAATGAT
TGGTTAATTAAATTTGACAATAAATATAAAATTGATATTTTAATTGCAAATGCTGGAACT
ATTGAAACAATTTTACCAAAAGAATTANATTTTACTGAAAGAATATTAACAGTTGCAAAC
ACAAATGTAATTGGAACATTGAATACAGTTTTACCAATGGTNCCAATCTTTGAATCNNNN
NNNNNN
Length of 5' end seq. 546
3' end seq. ID VFO635Z
3' end seq. 
>VFO635Z.Seq
NNNNNNNNNNGGTTAATTAAATTTGACAATAAATATAAAATTGATATTTTAATTGCAAAT
GCTGGAACTATTGAAACAATTTTACCAAAAGAATTAGATTTTACTGAAAGAATATTAACA
GTTGCAAACACAAATGTAATTGGAACATTGAATACAGTTTTACCAATGGTACCAATCTTT
GAATCAAGAGGATCTGGTCAATTGGTTTTGATGTCAAGTATTACACCATATTTTGATTAT
GTAATGGCAGGATATTCATCAAGTAAAGGTTACATAAAGTCATTTGGGTTAATACTTAGA
AATGGTTTAGCATCAAGAGGCGTTGGCGTTTCAGTAATAACACCAGGTTTTATTTATACT
CCATTAGTTGATTCTTTGGATTATAAGGATATACCTATGTTACTACCTATAACAACTGAC
AAAGCTTCAAAATACATTGTCGATGGCATTAGTAGAAATGATGCAATAATTTCATTCCCA
CCTTTAATTTATTGTATATCTCATTTCGTTGCCACAATTCCACCAACTTTAAGAGATGCC
ACTAATTTCATTTCAAATTCTCTTTTGAAATATCCAGATTATAGTAAACCAGTAACTCAT
TATTCTCATCACTCTCAATATTCAACAAATTAATCAAAAAGAGAGGATTGTCCCTCCTCA
CCAGCTANAATTCAAATTCTCAAAAAAATAAAGAGGCA
Length of 3' end seq. 698
Connected seq. ID VFO635P
Connected seq. 
>VFO635P.Seq
AAAAAAAATAAAAAATAATAAGAATGGGAGTCTTAACTGATATATCATTATTGTTGACCA
ATATAATTGGTTCAATAATTCTATTAATATTTTATCCTATATTAGGTTTTTTAAATTATA
AAAAAAAAGAAAACAAATTTCCCAACACCAAAAGCAATAGTTATCACGGGTGCATCATCT
GGTATTGGTAGAGGAATAGCACTTGAATATGCAAAAAGAAAAAAAAATAACTCTTTGGTT
TTAGGTTTAACTGGAAGAAACATTGAAAAATTAGAAGAAATTCAAAAAGAATGTTTGGGT
CTTGGNGTTCAAGTANAAATTGAATCAATCGATGTCACTGNCAAAGAAAAATTAAATGAT
TGGTTAATTAAATTTGACAATAAATATAAAATTGATATTTTAATTGCAAATGCTGGAACT
ATTGAAACAATTTTACCAAAAGAATTANATTTTACTGAAAGAATATTAACAGTTGCAAAC
ACAAATGTAATTGGAACATTGAATACAGTTTTACCAATGGTNCCAATCTTTGAATC----
------GGTTAATTAAATTTGACAATAAATATAAAATTGATATTTTAATTGCAAATGCTG
GAACTATTGAAACAATTTTACCAAAAGAATTAGATTTTACTGAAAGAATATTAACAGTTG
CAAACACAAATGTAATTGGAACATTGAATACAGTTTTACCAATGGTACCAATCTTTGAAT
CAAGAGGATCTGGTCAATTGGTTTTGATGTCAAGTATTACACCATATTTTGATTATGTAA
TGGCAGGATATTCATCAAGTAAAGGTTACATAAAGTCATTTGGGTTAATACTTAGAAATG
GTTTAGCATCAAGAGGCGTTGGCGTTTCAGTAATAACACCAGGTTTTATTTATACTCCAT
TAGTTGATTCTTTGGATTATAAGGATATACCTATGTTACTACCTATAACAACTGACAAAG
CTTCAAAATACATTGTCGATGGCATTAGTAGAAATGATGCAATAATTTCATTCCCACCTT
TAATTTATTGTATATCTCATTTCGTTGCCACAATTCCACCAACTTTAAGAGATGCCACTA
ATTTCATTTCAAATTCTCTTTTGAAATATCCAGATTATAGTAAACCAGTAACTCATTATT
CTCATCACTCTCAATATTCAACAAATTAATCAAAAAGAGAGGATTGTCCCTCCTCACCAG
CTANAATTCAAATTCTCAAAAAAATAAAGAGGCA
Length of connected seq. 1224
Full length Seq ID VFO635E
Full length Seq. 
>VFO635E.Seq
AAAAAAAATAAAAAATAATAAGAATGGGAGTCTTAACTGATATATCATTATTGTTGACCA
ATATAATTGGTTCAATAATTCTATTAATATTTTATCCTATATTAGGTTTTTTAAATTATA
AAAAAAAAGAAAACAAATTTCCCAACACCAAAAGCAATAGTTATCACGGGTGCATCATCT
GGTATTGGTAGAGGAATAGCACTTGAATATGCAAAAAGAAAAAAAAATAACTCTTTGGTT
TTAGGTTTAACTGGAAGAAACATTGAAAAATTAGAAGAAATTCAAAAAGAATGTTTGGGT
CTTGGNGTTCAAGTANAAATTGAATCAATCGATGTCACTGNCAAAGAAAAATTAAATGAT
TGGTTAATTAAATTTGACAATAAATATAAAATTGATATTTTAATTGCAAATGCTGGAACT
ATTGAAACAATTTTACCAAAAGAATTAGATTTTACTGAAAGAATATTAACAGTTGCAAAC
ACAAATGTAATTGGAACATTGAATACAGTTTTACCAATGGTACCAATCTTTGAATCAAGA
GGATCTGGTCAATTGGTTTTGATGTCAAGTATTACACCATATTTTGATTATGTAATGGCA
GGATATTCATCAAGTAAAGGTTACATAAAGTCATTTGGGTTAATACTTAGAAATGGTTTA
GCATCAAGAGGCGTTGGCGTTTCAGTAATAACACCAGGTTTTATTTATACTCCATTAGTT
GATTCTTTGGATTATAAGGATATACCTATGTTACTACCTATAACAACTGACAAAGCTTCA
AAATACATTGTCGATGGCATTAGTAGAAATGATGCAATAATTTCATTCCCACCTTTAATT
TATTGTATATCTCATTTCGTTGCCACAATTCCACCAACTTTAAGAGATGCCACTAATTTC
ATTTCAAATTCTCTTTTGAAATATCCAGATTATAGTAAACCAGTAACTCATTATTCTCAT
CACTCTCAATATTCAACAAATTAATCAAAAAGAGAGGATTGTCCCTCCTCACCAGCTANA
ATTCAAATTCTCAAAAAAATAAAGAGGCA
Length of full length seq. 1049