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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFO589
Library VF
(Link to library)
Clone ID VFO589
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFO589P
(Link to Original site)
Representative DNA sequence 
>VFO589 (VFO589Q) /CSM/VF/VFO5-D/VFO589Q.Seq.d/
AGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGCXXXXXXXXXXTTTCCGTATTGTCACTCCATTCCAA
ACTCGTTCATGGTATCATAAATATCAACACATTTACGCTCCACTCCTCTATGGTATTTAC
ACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCA
ATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCT
TTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGT
TATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGT
CATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAA
ATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCA
CTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCA
TCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAA
CATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAAT
TACCTTTACAAAATGGGTAATNATCCAGATTATGTAAAAAACCATAGCCTCAAAAGA
sequence update 2001.11.22
Translated Amino Acid sequence 
yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKEV
LLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRKH
FKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHM
MHDSCHA---

---FRIVTPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNF
DKAAYVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFF
ATPERPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQ
LVPIVKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNXPDYVKNHSLKR
Translated Amino Acid sequence (All Frames) 
Frame A:
sifctyinrivikcqk*lqesntlgqs*pnitlkmivglqsmvkfmilqdgyhyiqvvkk
fyf*qqvemlqiylkviiqcqinqhqf*rimkldifhhmniqnlfknqistrh*krelen
ilkqpikihkwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswqsfthfathysqci
*cmihvml---

---fpychsipnsfmvs*isthlrstplwylhtqisysrlgsfckgw*kwcnsc*crhkf
r*grlrhw*ivfcflpfhpstpls*lyrfnllfphc*irlwlvshn*fps*scr*rsqil
cyp*ktr*tisnq*rlgnpst*nysrlwswftplylf*wffkssscssfipincsrflpt
tctnckrsl*rt*hylph*tklh*syyvth*lplqng*xsrlckkp*pqk

Frame B:
vfsvhiliel**nvksnyrkaillvrvsqt*h*k*llgcsrw*sl*yykmgtitsrw*rs
ftfssr*rcykfi*klssnvr*tninfkel*nwiyfii*tskicskirflqdikres*kt
f*snrsrstngs*yfltfsiglsfsvcnllfstlyq*qflfklflgnrlrtlqliilnay
da*fmscc---

---FRIVTPFQTRSWYHKYQHIYAPLLYGIYTLKYRTQDWEAFVKDGKNGAIRVSVATNF
DKAAYVIGKLSFVFFRFILPLRYHSFTDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFF
ATPERPDEPSQINEDWAILQLKTTQDYGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQ
LVPIVKEVCKEHNITYHIKPNFTEAIMSHINYLYKMGNXPDYVKNHSLKR

Frame C:
yflyiy**NCNKMSKVITGKQYSWSELAKHNTENDCWVAVDGKVYDITRWVPLHPGGKEV
LLLAAGRDVTNLFESYHPMSDKPTSILKNYEIGYISSYEHPKFVQKSDFYKTLKERVRKH
FKATDQDPQMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHM
MHDSCHA---

---svlslhsklvhgiinintftlhssmvfthsnivlkigkll*rmvkmvqfvlvspqis
irpltslvncllfssvssfhsviialqi*fvissllnsslvgisqlisklvmslkisnsl
lplkdqmnhlksmkigqsfnlkllkimvmvhsfvpflvvl*iiklfiiyshqllkisthn
lyql*kkfvknitllttlnqtslkllchtlitftkwvxiqim*ktiask
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO589 (VFO589Q) /CSM/VF/VFO5-D/VFO589Q.Seq.d/                       2426   0.0
VFA388 (VFA388Q) /CSM/VF/VFA3-D/VFA388Q.Seq.d/                       1332   0.0
VFA219 (VFA219Q) /CSM/VF/VFA2-A/VFA219Q.Seq.d/                       1328   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1318   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1318   0.0
VFB864 (VFB864Q) /CSM/VF/VFB8-C/VFB864Q.Seq.d/                       1316   0.0
VFF269 (VFF269Q) /CSM/VF/VFF2-C/VFF269Q.Seq.d/                       1306   0.0
VFA320 (VFA320Q) /CSM/VF/VFA3-A/VFA320Q.Seq.d/                       1306   0.0
VFI746 (VFI746Q) /CSM/VF/VFI7-B/VFI746Q.Seq.d/                       1302   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1302   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ436303) Dictyostelium discoideum cDNA clone:ddv30a23, 3' ...  1346   0.0    1
(BJ428985) Dictyostelium discoideum cDNA clone:ddv1p21, 3' e...  1332   0.0    1
(BJ431124) Dictyostelium discoideum cDNA clone:ddv9o21, 3' e...  1291   0.0    1
(BJ429738) Dictyostelium discoideum cDNA clone:ddv4p16, 3' e...  1291   0.0    2
(CS204284) Sequence 45 from Patent EP1598422.                    1289   0.0    1
(BD092935) Methods and compositions for synthesis of long ch...  1289   0.0    1
(BD082645) Methods and compositions for synthesis of long ch...  1289   0.0    1
(BD082630) Methods and compositions for synthesis of long ch...  1289   0.0    1
(C25549) Dictyostelium discoideum slug cDNA, clone SLA256.       1289   0.0    1
(BJ435057) Dictyostelium discoideum cDNA clone:ddv25m24, 3' ...  1287   0.0    1
dna update 2009. 5.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   465   e-129
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   321   3e-86
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   270   6e-71
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   171   5e-41
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   162   2e-38
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   158   4e-37
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   156   1e-36
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   147   1e-33
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   144   7e-33
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    143   1e-32
protein update 2009. 6.25
PSORT 

 psg: 0.70  gvh: 0.24  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFO589 is cyt
5' end seq. ID VFO589F
5' end seq. 
>VFO589F.Seq
AGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGCNNNNNNNNNN
Length of 5' end seq. 575
3' end seq. ID VFO589Z
3' end seq. 
>VFO589Z.Seq
NNNNNNNNNNTTTCCGTATTGTCACTCCATTCCAAACTCGTTCATGGTATCATAAATATC
AACACATTTACGCTCCACTCCTCTATGGTATTTACACACTCAAATATCGTACTCAAGATT
GGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATT
TCGATAAGGCCGCTTACGTCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTC
CACTCCGTTATCATAGCTTTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCT
TTGGTTGGTATCTCACAATTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCT
TTGCTACCCCTGAAAGACCAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTC
AACTTAAAACTACTCAAGATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTT
CTTTAAATCATCAAGTTGTTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCAC
AACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAAC
CAAACTTCACTGAAGCTATTATGTCACACATTAATTACCTTTACAAAATGGGTAATNATC
CAGATTATGTAAAAAACCATAGCCTCAAAAGA
Length of 3' end seq. 692
Connected seq. ID VFO589P
Connected seq. 
>VFO589P.Seq
AGTATTTTCTGTACATATATTAATAGAATTGTAATAAAATGTCAAAAGTAATTACAGGAA
AGCAATACTCTTGGTCAGAGTTAGCCAAACATAACACTGAAAATGATTGTTGGGTTGCAG
TCGATGGTAAAGTTTATGATATTACAAGATGGGTACCATTACATCCAGGTGGTAAAGAAG
TTTTACTTTTAGCAGCAGGTAGAGATGTTACAAATTTATTTGAAAGTTATCATCCAATGT
CAGATAAACCAACATCAATTTTAAAGAATTATGAAATTGGATATATTTCATCATATGAAC
ATCCAAAATTTGTTCAAAAATCAGATTTCTACAAGACATTAAAAGAGAGAGTTAGAAAAC
ATTTTAAAGCAACCGATCAAGATCCACAAATGGCAGTTAGTATTTTCTCACGTTTAGCAT
TGGTTTATCTTTTAGTGTTTGTAACTTACTATTTAGCACATTATACCAGTAACAATTTTT
ATTTAAATTGTTTCTTGGCAATCGTTTACGCACTTTGCAACTCATTATTCTCAATGCATA
TGATGCATGATTCATGTCATGCTGC----------TTTCCGTATTGTCACTCCATTCCAA
ACTCGTTCATGGTATCATAAATATCAACACATTTACGCTCCACTCCTCTATGGTATTTAC
ACACTCAAATATCGTACTCAAGATTGGGAAGCTTTTGTAAAGGATGGTAAAAATGGTGCA
ATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAAATTGTCT
TTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTTAATTTGT
TATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCAAGTTAGT
CATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACCATCTCAA
ATCAATGAAGATTGGGCAATCCTTCAACTTAAAACTACTCAAGATTATGGTCATGGTTCA
CTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTTATTCCCA
TCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTGTAAAGAA
CATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACACATTAAT
TACCTTTACAAAATGGGTAATNATCCAGATTATGTAAAAAACCATAGCCTCAAAAGA
Length of connected seq. 1247
Full length Seq ID -
Full length Seq. -
Length of full length seq. -