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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFO324
Library VF
(Link to library)
Clone ID VFO324
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16430-1
Original site URL
Representative seq. ID VFO324P
(Link to Original site)
Representative DNA sequence 
>VFO324 (VFO324Q) /CSM/VF/VFO3-A/VFO324Q.Seq.d/
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAN
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTNTTTTTAAGANAAT
TGATTTCAAATGCATCTGATGCCTTANATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAANACTGCCAAAA
CTNTTACACTCATTGATAGNGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTNCTATTGCTCGTTCAGGTNCTAAAAACTTTATGGAACAATTACAAAGNGGXXXXXXXX
XXGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCAACAATTAAAAGAAT
ATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATTAGATGAAACCGAAG
ATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTA
AAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTCCTCTCAACTCGTCTTGCCAACTCAC
CATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAG
CTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAAACTTTAGAAT
TAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAA
CTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCAT
TAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCA
TTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACAACCAGTGATGATA
TTCCACCATTAGAAGAAAACGATGAACCATCTGAAATGGAAAAAGTCGACTAGATTTAAT
TACTAGCCCTTTTGCTATCAATTTTTTGGAATATTGTTATATTATTATAATAAAATAAAT
TTTAAAAAAAA
sequence update 2001.11.22
Translated Amino Acid sequence 
ftfrsyilihn*FYLFFFVXHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLRXL
ISNASDALXKIRYQSLTDASVLESKTELEIKIIPDXTAKTXTLIDXGIGMTKTDMVKNLG
XIARSGXKNFMEQLQX---

---EVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQDKAANEELLK
QVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSSMSSYMSSKKT
LELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFASRIHRMIKLG
LSIQDDSSATTEESTNTTTSDDIPPLEENDEPSEMEKVD*i*llalllsifwnivill**
nkf*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
tshlgpif*fiinficfflxnikkwlnhklnvshsklklislcplllilfiqikkxf*xn
*fqmhlmpxikfvtnh*lmhqfwnqklnsklklfqixlpkllhslixvlv*pkpiw*ri*
vlllvqvlktlwnnykx---

---EVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQDKAANEELLK
QVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSSMSSYMSSKKT
LELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFASRIHRMIKLG
LSIQDDSSATTEESTNTTTSDDIPPLEENDEPSEMEKVD*i*llalllsifwnivill**
nkf*kk

Frame B:
lhi*vlysns*lilfvffcxt*kng*its*tfhips*n*sayvpyy*yflfk*rsxfkxi
dfkci*clx*nslpiin*cisfgikn*trn*nysr*xcqnxyth**xywydqnrygkefr
xycsfrx*klygtitkx---

---ksftcvtqlmnmlfnn*knmtvrn*yqlpkkv*n*mkpkmkrkklnkiklpmknysn
klkmywviklkr*ssqlvlpthhvf*lplnmvgqqtwnvs*klklsvtlqchhtchqrkl
*n*iqitqslei*pkkppkkvklsktlftyymklls*lqvsh*mnhhhllhvfiv*ln*v
*afkmihqqqlknqqtqqpvmifhh*kktmnhlkwkkstrfny*pfcyqffgillyyynk
infkk

Frame C:
ftfrsyilihn*FYLFFFVXHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLRXL
ISNASDALXKIRYQSLTDASVLESKTELEIKIIPDXTAKTXTLIDXGIGMTKTDMVKNLG
XIARSGXKNFMEQLQX---

---shlhv*pn**iccstikri*r*eisinyqrrfeir*nrr*keks*tr*scq*ritqt
s*rcig**s*kgsplnsscqltmcfsyl*iwlvskhgtyhessssp*lfnviihvikenf
riksrspnr*rfsqksrrkk*nfqrlcllii*ncslnfrflir*tiiicftyssyd*irf
khsr*fisnn*rinkhnnq**ystirrkr*ti*ngksrldlitspfainfleycyiiiik
*ilkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO324 (VFO324Q) /CSM/VF/VFO3-A/VFO324Q.Seq.d/                       1324   0.0
VHQ442 (VHQ442Q) /CSM/VH/VHQ4-B/VHQ442Q.Seq.d/                        771   0.0
VHQ333 (VHQ333Q) /CSM/VH/VHQ3-B/VHQ333Q.Seq.d/                        771   0.0
VHP779 (VHP779Q) /CSM/VH/VHP7-D/VHP779Q.Seq.d/                        771   0.0
VHP202 (VHP202Q) /CSM/VH/VHP2-A/VHP202Q.Seq.d/                        771   0.0
VHP148 (VHP148Q) /CSM/VH/VHP1-B/VHP148Q.Seq.d/                        771   0.0
VHO658 (VHO658Q) /CSM/VH/VHO6-C/VHO658Q.Seq.d/                        771   0.0
VHN866 (VHN866Q) /CSM/VH/VHN8-C/VHN866Q.Seq.d/                        771   0.0
VHN670 (VHN670Q) /CSM/VH/VHN6-C/VHN670Q.Seq.d/                        771   0.0
VHN633 (VHN633Q) /CSM/VH/VHN6-B/VHN633Q.Seq.d/                        771   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ435978) Dictyostelium discoideum cDNA clone:ddv29p05, 3' ...   777   0.0    2
(L43591) Dictyostelium discoideum (clone C 6.1.1) heat shock...   769   0.0    2
(BJ446614) Dictyostelium discoideum cDNA clone:ddv63d12, 3' ...   769   0.0    4
(BJ446603) Dictyostelium discoideum cDNA clone:ddv63b09, 3' ...   769   0.0    2
(BJ446503) Dictyostelium discoideum cDNA clone:ddv62n19, 3' ...   769   0.0    2
(BJ446041) Dictyostelium discoideum cDNA clone:ddv61o11, 3' ...   769   0.0    3
(BJ445907) Dictyostelium discoideum cDNA clone:ddv61c02, 3' ...   769   0.0    2
(BJ445748) Dictyostelium discoideum cDNA clone:ddv60c16, 3' ...   769   0.0    2
(BJ445166) Dictyostelium discoideum cDNA clone:ddv58k18, 3' ...   769   0.0    4
(BJ445137) Dictyostelium discoideum cDNA clone:ddv58e17, 3' ...   769   0.0    2
dna update 2009. 5.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             303   7e-81
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   303   7e-81
AB092411_1(AB092411|pid:none) Entamoeba histolytica gene for cyt...   213   1e-53
DQ884433_1(DQ884433|pid:none) Karlodinium micrum clone Kmi-5p-cD...   211   4e-53
AY292370_1(AY292370|pid:none) Toxoplasma gondii HSP90 mRNA, comp...   209   1e-52
DQ062086_1(DQ062086|pid:none) Pseudourostyla cristata heat shock...   209   2e-52
AY831450_1(AY831450|pid:none) Monocercomonoides sp. PA203 heat s...   209   2e-52
AF402101_1(AF402101|pid:none) Achlya ambisexualis isolate JS855 ...   205   3e-51
AF402100_1(AF402100|pid:none) Achlya ambisexualis isolate JS854 ...   205   3e-51
EU780016_1(EU780016|pid:none) Fucus serratus isolate Fse_00015 h...   204   4e-51
protein update 2009. 6.25
PSORT 

 psg: 0.50  gvh: 0.53  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFO324 is cyt
5' end seq. ID VFO324F
5' end seq. 
>VFO324F.Seq
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAN
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTNTTTTTAAGANAAT
TGATTTCAAATGCATCTGATGCCTTANATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAANACTGCCAAAA
CTNTTACACTCATTGATAGNGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTNCTATTGCTCGTTCAGGTNCTAAAAACTTTATGGAACAATTACAAAGNGGNNNNNNNN
NN
Length of 5' end seq. 422
3' end seq. ID VFO324Z
3' end seq. 
>VFO324Z.Seq
NNNNNNNNNNGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCAACAATT
AAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATTAGATGA
AACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTCAA
ACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTCCTCTCAACTCGTCTTGC
CAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGTAT
CATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAAAC
TTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAAA
AAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCAGG
TTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTAGG
TTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACAACCAG
TGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAATGGAAAAAGTCGACTA
GATTTAATTACTAGCCCTTTTGCTATCAATTTTTTGGAATATTGTTATATTATTATAATA
AAATAAATTTTAAAAAAAA
Length of 3' end seq. 739
Connected seq. ID VFO324P
Connected seq. 
>VFO324P.Seq
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAN
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTNTTTTTAAGANAAT
TGATTTCAAATGCATCTGATGCCTTANATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAANACTGCCAAAA
CTNTTACACTCATTGATAGNGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTNCTATTGCTCGTTCAGGTNCTAAAAACTTTATGGAACAATTACAAAGNGG--------
--GAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCAACAATTAAAAGAAT
ATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATTAGATGAAACCGAAG
ATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTACTCAAACAAGTTA
AAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTCCTCTCAACTCGTCTTGCCAACTCAC
CATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAG
CTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAGAAAACTTTAGAAT
TAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAA
CTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCAT
TAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCA
TTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACAACCAGTGATGATA
TTCCACCATTAGAAGAAAACGATGAACCATCTGAAATGGAAAAAGTCGACTAGATTTAAT
TACTAGCCCTTTTGCTATCAATTTTTTGGAATATTGTTATATTATTATAATAAAATAAAT
TTTAAAAAAAA
Length of connected seq. 1141
Full length Seq ID -
Full length Seq. -
Length of full length seq. -